目的探讨脑小血管病(CSVD)患者外周血G蛋白耦联雌激素受体30(GPER30)、神经元PAS结构域蛋白4(NPAS4)、FK506结合蛋白5(FKBP5)表达与认知功能障碍(CD)的相关性。方法前瞻性选取2022年1月至2023年12月郑州大学第二附属医院收治的227例CSV...目的探讨脑小血管病(CSVD)患者外周血G蛋白耦联雌激素受体30(GPER30)、神经元PAS结构域蛋白4(NPAS4)、FK506结合蛋白5(FKBP5)表达与认知功能障碍(CD)的相关性。方法前瞻性选取2022年1月至2023年12月郑州大学第二附属医院收治的227例CSVD患者,根据有无CD分为障碍组(n=66)与无障碍组(n=161)。比较两组患者的一般资料及外周血GPER30、NPAS4、FKBP5 m RNA表达水平,Logistic回归分析CSVD患者CD的影响因素,比较不同程度CD患者外周血GPER30、NPAS4、FKBP5 m RNA表达水平,采用Pearson法分析外周血GPER30、NPAS4、FKBP5 m RNA表达与蒙特利尔认知评估量表(Mo CA)评分的相关性。结果障碍组患者的年龄、病程分别为(72.49±5.68)岁、(2.69±0.78)年,明显高(长)于无障碍组的(67.51±7.04)岁、(2.31±0.62)年,差异均有统计学意义(P<0.05);障碍组患者的外周血GPER30 m RNA表达水平为1.02±0.17,明显低于无障碍组的1.66±0.31,NPAS4m RNA、FKBP5 m RNA表达水平分别为2.79±0.60、3.88±1.12,明显高于无障碍组的1.55±0.51、2.10±0.59,差异均有统计学意义(P<0.05);Logistic回归分析结果显示,年龄、病程、GPER30 m RNA、NPAS4 m RNA及FKBP5 m RNA均为CSVD患者CD的独立影响因素(P<0.05)。轻度组患者的外周血GPER30 m RNA表达水平为1.27±0.25,明显高于中重度组的0.70±0.12,NPAS4 m RNA、FKBP5 m RNA表达水平分别为2.31±0.58、3.19±1.07,明显低于中重度组的3.40±0.72、4.76±1.39,差异均有统计学意义(P<0.05);Pearson法分析结果显示,外周血GPER30 m RNA表达与CSVD患者Mo CA评分呈正相关(r=0.704,P<0.05),NPAS4 m RNA、FKBP5 m RNA与Mo CA评分呈负相关(r=-0.572、-0.542,P<0.05)。结论外周血GPER30、NPAS4、FKBP5是CSVD患者CD的独立相关因素,各指标表达水平与CD病情严重程度均具有一定相关性,可为临床判断CD、评估CD病情严重程度提供参考,以指导后续临床工作。展开更多
Dear Editor,In dynamic environments,the memory system of the brain must be able to perceive and process conflicting experiences to reach an adaptive decision.In Drosophila,in contrast to consistent experiences,conflic...Dear Editor,In dynamic environments,the memory system of the brain must be able to perceive and process conflicting experiences to reach an adaptive decision.In Drosophila,in contrast to consistent experiences,conflicting experiences trigger significantly increased Rac1 activity which mediates active forgetting [1].The ability to cope with conflicting experiences but not simple learning experiences is impaired in mutants of multiple autism-risk genes [2].展开更多
Purpose:Post-stroke depression(PSD)is a frequent neuropsychiatric disorder following stroke which is associated with poor outcome.Neuronal Per-Arnt-Sim(PAS)domain protein 4(Npas4)is associated with cognitive function....Purpose:Post-stroke depression(PSD)is a frequent neuropsychiatric disorder following stroke which is associated with poor outcome.Neuronal Per-Arnt-Sim(PAS)domain protein 4(Npas4)is associated with cognitive function.Npas4 expression in peripheral blood mononuclear cells(PBMCs)from patients with PSD was measured to find new therapeutic strategy.Patients and methods:Ischemic stroke patients(n=152)within 1 week of stroke onset were recruited.At 3 months follow-up,the patients were divided into a PSD group(n=77)and a stroke group(n=75)using the Hamilton Rating Scale.Healthy subjects(n=75)were also recruited in the study.The PSD group received 12 weeks of duloxetine treatment.Cognitive function was evaluated using the P300 test.Npas4 expression in PBMCs was measured by quantitative RT-PCR(qPCR).Results:Before treatment,P300 latencies in the PSD group were prolonged and the P300 amplitudes were lower than the control group(P<0.01).Npas4 expression in the PSD group was also lower than the control group(P<0.01).After treatment,the P300 latencies were reduced and the amplitudes were significantly elevated in the PSD group compared to that before treatment(P<0.01).Meanwhile,Npas4 levels were significantly higher than that before treatment(P<0.01).Npas4 expression was positively correlated to the P300 amplitudes(P<0.05).Conclusion:Changes of Npas4 expression in PBMCs are associated with cognitive impairment in PSD patients and new therapeutic options applying Npas4-related transcript mechanism could be considered in the future.展开更多
文摘目的探讨脑小血管病(CSVD)患者外周血G蛋白耦联雌激素受体30(GPER30)、神经元PAS结构域蛋白4(NPAS4)、FK506结合蛋白5(FKBP5)表达与认知功能障碍(CD)的相关性。方法前瞻性选取2022年1月至2023年12月郑州大学第二附属医院收治的227例CSVD患者,根据有无CD分为障碍组(n=66)与无障碍组(n=161)。比较两组患者的一般资料及外周血GPER30、NPAS4、FKBP5 m RNA表达水平,Logistic回归分析CSVD患者CD的影响因素,比较不同程度CD患者外周血GPER30、NPAS4、FKBP5 m RNA表达水平,采用Pearson法分析外周血GPER30、NPAS4、FKBP5 m RNA表达与蒙特利尔认知评估量表(Mo CA)评分的相关性。结果障碍组患者的年龄、病程分别为(72.49±5.68)岁、(2.69±0.78)年,明显高(长)于无障碍组的(67.51±7.04)岁、(2.31±0.62)年,差异均有统计学意义(P<0.05);障碍组患者的外周血GPER30 m RNA表达水平为1.02±0.17,明显低于无障碍组的1.66±0.31,NPAS4m RNA、FKBP5 m RNA表达水平分别为2.79±0.60、3.88±1.12,明显高于无障碍组的1.55±0.51、2.10±0.59,差异均有统计学意义(P<0.05);Logistic回归分析结果显示,年龄、病程、GPER30 m RNA、NPAS4 m RNA及FKBP5 m RNA均为CSVD患者CD的独立影响因素(P<0.05)。轻度组患者的外周血GPER30 m RNA表达水平为1.27±0.25,明显高于中重度组的0.70±0.12,NPAS4 m RNA、FKBP5 m RNA表达水平分别为2.31±0.58、3.19±1.07,明显低于中重度组的3.40±0.72、4.76±1.39,差异均有统计学意义(P<0.05);Pearson法分析结果显示,外周血GPER30 m RNA表达与CSVD患者Mo CA评分呈正相关(r=0.704,P<0.05),NPAS4 m RNA、FKBP5 m RNA与Mo CA评分呈负相关(r=-0.572、-0.542,P<0.05)。结论外周血GPER30、NPAS4、FKBP5是CSVD患者CD的独立相关因素,各指标表达水平与CD病情严重程度均具有一定相关性,可为临床判断CD、评估CD病情严重程度提供参考,以指导后续临床工作。
基金supported by grants from the National Natural Science Foundation of China (31970955 and 31700912)。
文摘Dear Editor,In dynamic environments,the memory system of the brain must be able to perceive and process conflicting experiences to reach an adaptive decision.In Drosophila,in contrast to consistent experiences,conflicting experiences trigger significantly increased Rac1 activity which mediates active forgetting [1].The ability to cope with conflicting experiences but not simple learning experiences is impaired in mutants of multiple autism-risk genes [2].
基金supported by Natural Science Foundation of Henan Province(Grant No.162300410224)Open Issue in Key Biological Psychiatry Laboratory of Henan Province(Grant No.ZDSYS2016004)+5 种基金Medical Science Research Projects in Henan Province in 2018(Grant No.2018010016)Key Research Projects(Grant No.14B320016)Foundation and Research in Cuttingedge Technologies Plan Projects of Henan Province(Grant No.132300410162)Key Scientific Research Projects of Universities in Henan Province(Grant No.16A320044)Science and Technology Plan Projects of Henan Province(Grant No.162102310489)Natural Science Foundation of Henan Provience(Grant No.81471349).
文摘Purpose:Post-stroke depression(PSD)is a frequent neuropsychiatric disorder following stroke which is associated with poor outcome.Neuronal Per-Arnt-Sim(PAS)domain protein 4(Npas4)is associated with cognitive function.Npas4 expression in peripheral blood mononuclear cells(PBMCs)from patients with PSD was measured to find new therapeutic strategy.Patients and methods:Ischemic stroke patients(n=152)within 1 week of stroke onset were recruited.At 3 months follow-up,the patients were divided into a PSD group(n=77)and a stroke group(n=75)using the Hamilton Rating Scale.Healthy subjects(n=75)were also recruited in the study.The PSD group received 12 weeks of duloxetine treatment.Cognitive function was evaluated using the P300 test.Npas4 expression in PBMCs was measured by quantitative RT-PCR(qPCR).Results:Before treatment,P300 latencies in the PSD group were prolonged and the P300 amplitudes were lower than the control group(P<0.01).Npas4 expression in the PSD group was also lower than the control group(P<0.01).After treatment,the P300 latencies were reduced and the amplitudes were significantly elevated in the PSD group compared to that before treatment(P<0.01).Meanwhile,Npas4 levels were significantly higher than that before treatment(P<0.01).Npas4 expression was positively correlated to the P300 amplitudes(P<0.05).Conclusion:Changes of Npas4 expression in PBMCs are associated with cognitive impairment in PSD patients and new therapeutic options applying Npas4-related transcript mechanism could be considered in the future.
