目的确定NP9基因的表达对鼻咽癌细胞裸鼠成瘤的影响,并探讨其机制。方法构建NP9基因的真核表达载体,转染重组pR c/CMV2-NP9质粒于鼻咽癌细胞系CNE1和SUNE1,筛选并获得稳定表达NP9基因的细胞克隆CEN1/NP9和SUNE1/NP9。以裸鼠致瘤实验确定...目的确定NP9基因的表达对鼻咽癌细胞裸鼠成瘤的影响,并探讨其机制。方法构建NP9基因的真核表达载体,转染重组pR c/CMV2-NP9质粒于鼻咽癌细胞系CNE1和SUNE1,筛选并获得稳定表达NP9基因的细胞克隆CEN1/NP9和SUNE1/NP9。以裸鼠致瘤实验确定NP9基因对鼻咽癌细胞成瘤特性的影响;通过免疫组化检测移植瘤中增殖细胞核抗原(PCNA)和细胞周期素D1(cyc lin D1)的表达。结果大部分转基因的G418抗性克隆能够检测到NP9基因的表达。CNE1/NP9细胞接种裸鼠后的成瘤率为43.8%,明显低于CNE1细胞(66.7%)和CNE1/空载体组(75.0%,P均<0.05)。SUNE1/NP9细胞接种裸鼠后移植瘤的生长速度减慢,第30天时肿瘤体积为(3.88±0.81)cm3,与SUNE1细胞和SUNE1/空载体组差异均有统计学意义(P均<0.05)。CNE1/NP9细胞的移植瘤显示出相对较低的PCNA和cyc lin D1表达水平。结论NP9基因通过下调PCNA和cyc linD1的表达,抑制鼻咽癌细胞的裸鼠成瘤或移植瘤的生长。展开更多
Human endogenous retrovirus(HERV)gene sequences are remnants of retroviruses that infected the ancestors of humans millions of years ago and were integrated into human chromosomes,accounting for approximately 8%-9%of ...Human endogenous retrovirus(HERV)gene sequences are remnants of retroviruses that infected the ancestors of humans millions of years ago and were integrated into human chromosomes,accounting for approximately 8%-9%of the human genome.Most integrated HERVs have lost their coding capacity and remain silent due to frame shifts,mutations,and sequence deletions or insertions over the millions of years,but their expression is highly regulated by epigenetic and host defense mechanisms.However,there are still some HERV genes that have intact open reading frames due to recent integration into the human genome or positive selective pressure.The abnormal activation of HERVs may contribute to diseases or their pathology,such as malignant tumors,autoimmune diseases,and nervous system diseases.The occurrence and development of hematological malignant tumors(HMTs)is a complex process involving interactions of multiple genetic and environmental factors.The abnormal activation of HERVs may contribute to the pathology of HMTs via indirect mechanisms.In this review,we address the discovery of endogenous retroviruses in vertebrates,and the classification and genomic structure of HERVs.Among HERV family members,HERV-K is the latest type of HERV integrated into the human genome and it has the strongest transcriptional activity.We explore the currently known expression of HERV-K proto-oncogenes in HMTs and further address potential research and therapeutic approaches.However,much remains to be learned about not only the impact of HERVs on the occurrence of HMTs,but also the potential value of HERVs as diagnostic and therapeutic targets for HMTs.展开更多
文摘目的确定NP9基因的表达对鼻咽癌细胞裸鼠成瘤的影响,并探讨其机制。方法构建NP9基因的真核表达载体,转染重组pR c/CMV2-NP9质粒于鼻咽癌细胞系CNE1和SUNE1,筛选并获得稳定表达NP9基因的细胞克隆CEN1/NP9和SUNE1/NP9。以裸鼠致瘤实验确定NP9基因对鼻咽癌细胞成瘤特性的影响;通过免疫组化检测移植瘤中增殖细胞核抗原(PCNA)和细胞周期素D1(cyc lin D1)的表达。结果大部分转基因的G418抗性克隆能够检测到NP9基因的表达。CNE1/NP9细胞接种裸鼠后的成瘤率为43.8%,明显低于CNE1细胞(66.7%)和CNE1/空载体组(75.0%,P均<0.05)。SUNE1/NP9细胞接种裸鼠后移植瘤的生长速度减慢,第30天时肿瘤体积为(3.88±0.81)cm3,与SUNE1细胞和SUNE1/空载体组差异均有统计学意义(P均<0.05)。CNE1/NP9细胞的移植瘤显示出相对较低的PCNA和cyc lin D1表达水平。结论NP9基因通过下调PCNA和cyc linD1的表达,抑制鼻咽癌细胞的裸鼠成瘤或移植瘤的生长。
文摘Human endogenous retrovirus(HERV)gene sequences are remnants of retroviruses that infected the ancestors of humans millions of years ago and were integrated into human chromosomes,accounting for approximately 8%-9%of the human genome.Most integrated HERVs have lost their coding capacity and remain silent due to frame shifts,mutations,and sequence deletions or insertions over the millions of years,but their expression is highly regulated by epigenetic and host defense mechanisms.However,there are still some HERV genes that have intact open reading frames due to recent integration into the human genome or positive selective pressure.The abnormal activation of HERVs may contribute to diseases or their pathology,such as malignant tumors,autoimmune diseases,and nervous system diseases.The occurrence and development of hematological malignant tumors(HMTs)is a complex process involving interactions of multiple genetic and environmental factors.The abnormal activation of HERVs may contribute to the pathology of HMTs via indirect mechanisms.In this review,we address the discovery of endogenous retroviruses in vertebrates,and the classification and genomic structure of HERVs.Among HERV family members,HERV-K is the latest type of HERV integrated into the human genome and it has the strongest transcriptional activity.We explore the currently known expression of HERV-K proto-oncogenes in HMTs and further address potential research and therapeutic approaches.However,much remains to be learned about not only the impact of HERVs on the occurrence of HMTs,but also the potential value of HERVs as diagnostic and therapeutic targets for HMTs.
