[Objectives]To make full use of crop rhizosphere microbial resources.[Methods]Illumina NovaSeq sequencing platform was used to analyze the richness and diversity of microbial community structure in rhizosphere soil of...[Objectives]To make full use of crop rhizosphere microbial resources.[Methods]Illumina NovaSeq sequencing platform was used to analyze the richness and diversity of microbial community structure in rhizosphere soil of rice and maize crops in Baitu Town,Gaoyao District,Zhaoqing City.[Results]A total of 14936 OTUs of bacteria and 1905 OTUs of fungi were obtained from three samples of rice rhizosphere soil,and 13437 OTUs of bacteria and 1413 OTUs of fungi were obtained from three samples of maize rhizosphere soil.The diversity and richness of bacterial communities were higher than those of fungi.There are differences in soil bacterial and fungal communities among different crop samples.The analysis of species with bacteria difference at genus level among crop rhizosphere soil samples showed that 18 genera with significant differences were obtained from 6 samples;species analysis of fungi at the genus level showed that 3 genera with significant differences were obtained from 6 samples.[Conclusions]The research results of this paper have positive significance for the development and utilization of soil resources in Zhaoqing City and the full exploitation of rice and maize rhizosphere microbial resources.展开更多
In environmental biosafety assessments of glyphosate-tolerant crops,it is essential to evaluate the effects of cultivating these crops and applying glyphosate on the microbial community in the rhizosphere soil,which p...In environmental biosafety assessments of glyphosate-tolerant crops,it is essential to evaluate the effects of cultivating these crops and applying glyphosate on the microbial community in the rhizosphere soil,which play a critical role in maintaining soil health,plant growth,and crop productivity.Maize(Zea mays)line GG2 was previously generated by transforming wild-type maize with the gat and gr79-epsps genes,endowing GG2 with both active and passive resistance to glyphosate.However,the ecological risk of introducing these two new glyphosate-tolerance genes into maize,as well as glyphosate treatment,to rhizosphere microorganisms remain unclear.In this study,we used high-throughput sequencing to analyze the diversity and composition of the bacterial and fungal communities in the rhizosphere soil around biotech maize GG2,with(GG2-H)and without glyphosate treatment(GG2-N),compared with the near-isogenic,non-biotech maize line ZD958 at seven stages of growth.The structure and diversity of the bacterial and fungal communities of GG2-H were similar to those of ZD958,whereas glyphosate treatment had temporary effects on bacterial and fungal diversity and richness.The differences in the bacterial and fungal communities were associated with changes in soil properties such as pH,available phosphorus and organic matter,and seasonal changes.These factors,rather than maize lines,made the greatest contributions to the shifts in bacterial and fungal community structure.This study provides a comprehensive analysis of the effects of biotech crop cultivation,glyphosate treatment,soil physicochemical properties of soil,and maize growth stages on soil microbial communities,offering valuable insights for the large-scale adoption of biotech crops in China.展开更多
基金Supported by Guangdong Province Rural Science and Technology Commissioner Project(KTP20240693)Zhaoqing University Project(QN202329)+3 种基金Science and Technology Innovation Guidance Project of Zhaoqing(202304038001)Undergraduate Innovation and Entrepreneurship Training Program(202410580011&X202310580120)The Third Batch of Innovation Research Team of Zhaoqing University(05)Quality Engineering and Teaching Reform Project of Zhaoqing University(zlgc202229,zlgc202261).
文摘[Objectives]To make full use of crop rhizosphere microbial resources.[Methods]Illumina NovaSeq sequencing platform was used to analyze the richness and diversity of microbial community structure in rhizosphere soil of rice and maize crops in Baitu Town,Gaoyao District,Zhaoqing City.[Results]A total of 14936 OTUs of bacteria and 1905 OTUs of fungi were obtained from three samples of rice rhizosphere soil,and 13437 OTUs of bacteria and 1413 OTUs of fungi were obtained from three samples of maize rhizosphere soil.The diversity and richness of bacterial communities were higher than those of fungi.There are differences in soil bacterial and fungal communities among different crop samples.The analysis of species with bacteria difference at genus level among crop rhizosphere soil samples showed that 18 genera with significant differences were obtained from 6 samples;species analysis of fungi at the genus level showed that 3 genera with significant differences were obtained from 6 samples.[Conclusions]The research results of this paper have positive significance for the development and utilization of soil resources in Zhaoqing City and the full exploitation of rice and maize rhizosphere microbial resources.
基金supported by the Nanfan Special Project(Grant No.YBXM2318)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202402)the Biological Breeding-National Science and Technology Major Project(2022ZD0400602).
文摘In environmental biosafety assessments of glyphosate-tolerant crops,it is essential to evaluate the effects of cultivating these crops and applying glyphosate on the microbial community in the rhizosphere soil,which play a critical role in maintaining soil health,plant growth,and crop productivity.Maize(Zea mays)line GG2 was previously generated by transforming wild-type maize with the gat and gr79-epsps genes,endowing GG2 with both active and passive resistance to glyphosate.However,the ecological risk of introducing these two new glyphosate-tolerance genes into maize,as well as glyphosate treatment,to rhizosphere microorganisms remain unclear.In this study,we used high-throughput sequencing to analyze the diversity and composition of the bacterial and fungal communities in the rhizosphere soil around biotech maize GG2,with(GG2-H)and without glyphosate treatment(GG2-N),compared with the near-isogenic,non-biotech maize line ZD958 at seven stages of growth.The structure and diversity of the bacterial and fungal communities of GG2-H were similar to those of ZD958,whereas glyphosate treatment had temporary effects on bacterial and fungal diversity and richness.The differences in the bacterial and fungal communities were associated with changes in soil properties such as pH,available phosphorus and organic matter,and seasonal changes.These factors,rather than maize lines,made the greatest contributions to the shifts in bacterial and fungal community structure.This study provides a comprehensive analysis of the effects of biotech crop cultivation,glyphosate treatment,soil physicochemical properties of soil,and maize growth stages on soil microbial communities,offering valuable insights for the large-scale adoption of biotech crops in China.
