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Norming Subspaces Isomorphic to l_1
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作者 Sofiya OSTROVSKA 《Acta Mathematica Sinica,English Series》 SCIE CSCD 2015年第5期767-771,共5页
Norming subspaces are studied widely in the duality theory of Banach spaces. These subspaces are applied to the Borel and Baire classifications of the inverse operators. The main result of this article asserts that th... Norming subspaces are studied widely in the duality theory of Banach spaces. These subspaces are applied to the Borel and Baire classifications of the inverse operators. The main result of this article asserts that the dual of a Banach space X contains a norming subspace isomorphic to l1 provided that the following two conditions are satisfied: (1) X* contains a subspace isomorphic to l1; and (2) X* contains a separable norming subspace. 展开更多
关键词 Banach space norming subspace dual space weak* convergence free ultrafilter
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Real Renormings on Complex Banach Spaces
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作者 Francisco J GARC■A-PACHECO Alejandro MIRALLES 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2008年第3期239-246,共8页
The authors prove that every complex Banach space admits an equivalent real norm that is far away from being a complex norm. Furthermore, this real norm can be chosen to share many properties with complex norms, but i... The authors prove that every complex Banach space admits an equivalent real norm that is far away from being a complex norm. Furthermore, this real norm can be chosen to share many properties with complex norms, but it is still not a complex norm. 展开更多
关键词 Complex norms Real norms Hilbert space
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有限群的幂自同构与子群格
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作者 郭秀云 王俊新 《数学进展》 北大核心 2025年第2期357-378,共22页
群与格是现代数学中两个最基本的研究对象.本文主要介绍群的幂自同构与群的子群格之间的相互关联,以及近年来围绕群的幂自同构与群的子群格方面人们所取得的一些新的研究成果.我们将从有限群的幂自同构与有限群的结构,有限群的norm、Wie... 群与格是现代数学中两个最基本的研究对象.本文主要介绍群的幂自同构与群的子群格之间的相互关联,以及近年来围绕群的幂自同构与群的子群格方面人们所取得的一些新的研究成果.我们将从有限群的幂自同构与有限群的结构,有限群的norm、Wielandt-子群以及Wielandt-子群链与有限群的结构,有限群的子群格与有限群的结构,有限群的二极大子群、弱二极大子群与有限群的结构等四个方面来阐述.同时,一些可以进一步研究的问题被提出. 展开更多
关键词 幂自同构 子群格 NORM Wielandt-子群链 极大子群 二极大子群 弱二极大子群 Pálfy-Pudlák问题
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Infrared small target detection algorithm via partial sum of the tensor nuclear norm and direction residual weighting
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作者 SUN Bin XIA Xing-Ling +1 位作者 FU Rong-Guo SHI Liang 《红外与毫米波学报》 北大核心 2025年第2期277-288,共12页
Aiming at the problem that infrared small target detection faces low contrast between the background and the target and insufficient noise suppression ability under the complex cloud background,an infrared small targe... Aiming at the problem that infrared small target detection faces low contrast between the background and the target and insufficient noise suppression ability under the complex cloud background,an infrared small target detection method based on the tensor nuclear norm and direction residual weighting was proposed.Based on converting the infrared image into an infrared patch tensor model,from the perspective of the low-rank nature of the background tensor,and taking advantage of the difference in contrast between the background and the target in different directions,we designed a double-neighborhood local contrast based on direction residual weighting method(DNLCDRW)combined with the partial sum of tensor nuclear norm(PSTNN)to achieve effective background suppression and recovery of infrared small targets.Experiments show that the algorithm is effective in suppressing the background and improving the detection ability of the target. 展开更多
关键词 infrared small target detection infrared patch tensor model partial sum of the tensor nuclear norm direction residual weighting
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Ecological Assessment of Physicochemical Pollution of Wadi Sebou(Gharb,Morocco)
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作者 Habiby El Moctar Fatimetou Salma +9 位作者 Brahim Ahmed Dick Ilyass Laazar Ettaki Jalal Aalama Fatima Hraira Thami Hammouali Kamal Maaroufi Manal Echayeb Hajar Fariri Khalid Belghyti Driss 《Journal of Environmental & Earth Sciences》 2025年第6期351-369,共19页
In Morocco,hydrological and hydrobiological surveys of wadis,river and stream are rare.In this research,we plan to attempt to characterize the physico-chemistry of surface waters of sixteen stations sampled at the lev... In Morocco,hydrological and hydrobiological surveys of wadis,river and stream are rare.In this research,we plan to attempt to characterize the physico-chemistry of surface waters of sixteen stations sampled at the level of the Sebou basin during the period 2013-2023.The diagnosis showed that this natural watercourse is highly enriched in oce-anic salts.A large fluctuation in the chemical nature of the waters was recorded,as well as electrical conductivity(EC),which oscillates between 629 and 22,766μS/cm.Hydrogen Potential ranges from 8.01 to 8.79 while remaining basic.Nitrate(NO3-)concentrations range from a maximum of 886.9 mg/l to a minimum of 0.24 mg/l.Similarly,the am-monium concentration varies from 0.04 to 15.34 mg/l.It was also noted that the waters were very rich in chloride ions(860.27 to 145.55 mg/l),in sodium Na+ion(51 to 2,530 mg/l),in sulfate ions SO4--(441.4 to 37.62 mg/l),in calcium ions Ca2+(97.6 to 1,072.8 mg/l)and in magnesium ions Mg2+(631.2 to 17.28 mg/l)which explains the high hardness of these waters.The concentrations of potassium ions(K+)range from 2.54 to 17.55 mg/l.The high alkalinity is due to the high concentrations of bicarbonate ions(75.64 to 362.34 mg/l).Our study concludes that the waters of the Sebou re-main below the irrigation threshold authorized by Moroccan law when moving away from its Atlantic estuary.They are too degraded and we suggest that urban and industrial wastewater be pre-treated as a priority to reduce natural environment. 展开更多
关键词 Watercourse Physicochemistry Norme QUALITY Sebou Kenitra Morocco
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Bayesian Network Reconstruction and Iterative Divergence Problem Solving Method Based on Norm Minimization
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作者 Kuo Li Aimin Wang +2 位作者 Limin Wang Yuetan Zhao Xinyu Zhu 《Computer Modeling in Engineering & Sciences》 2025年第4期617-637,共21页
A Bayesian network reconstruction method based on norm minimization is proposed to address the sparsity and iterative divergence issues in network reconstruction caused by noise and missing values.This method achieves... A Bayesian network reconstruction method based on norm minimization is proposed to address the sparsity and iterative divergence issues in network reconstruction caused by noise and missing values.This method achieves precise adjustment of the network structure by constructing a preliminary random network model and introducing small-world network characteristics and combines L1 norm minimization regularization techniques to control model complexity and optimize the inference process of variable dependencies.In the experiment of game network reconstruction,when the success rate of the L1 norm minimization model’s existence connection reconstruction reaches 100%,the minimum data required is about 40%,while the minimum data required for a sparse Bayesian learning network is about 45%.In terms of operational efficiency,the running time for minimizing the L1 normis basically maintained at 1.0 s,while the success rate of connection reconstruction increases significantly with an increase in data volume,reaching a maximum of 13.2 s.Meanwhile,in the case of a signal-to-noise ratio of 10 dB,the L1 model achieves a 100% success rate in the reconstruction of existing connections,while the sparse Bayesian network had the highest success rate of 90% in the reconstruction of non-existent connections.In the analysis of actual cases,the maximum lift and drop track of the research method is 0.08 m.The mean square error is 5.74 cm^(2).The results indicate that this norm minimization-based method has good performance in data efficiency and model stability,effectively reducing the impact of outliers on the reconstruction results to more accurately reflect the actual situation. 展开更多
关键词 Bayesian norm minimization network reconstruction iterative divergence SPARSITY
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Generative Artificial Intelligence in Judicial Applications:Functions,Risks,and Controls
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作者 Hong Wenxiao 《Contemporary Social Sciences》 2025年第1期142-155,共14页
The application of generative artificial intelligence(GAI)in the judicial domain represents an innovative approach to achieving digital justice.GAI’s functionalities are primarily exhibited in the enhancement of judi... The application of generative artificial intelligence(GAI)in the judicial domain represents an innovative approach to achieving digital justice.GAI’s functionalities are primarily exhibited in the enhancement of judicial efficiency,improving the accuracy and fairness of adjudications,promoting the uniform application of the law,and enhancing transparency and engagement in judicial processes.However,these advancements are accompanied by potential risks and challenges in technology,adjudication,and ethics.It is thus essential to strengthen privacy protection during the processing and transmission of data,ensure data security and confidentiality,and establish and refine clear legal norms regarding the roles,responsibilities,and legal obligations of GAI in judicial settings.In addition,ethical guidelines that delineate the behavioral boundaries and value orientations of GAI need to be formulated.Collectively,these elements can form a trinary model of technical controls,legal norms,and ethical constraints,to facilitate the modernization and intelligent development of the judicial system. 展开更多
关键词 GAI application functions RISKS technical controls legal norms ethical constraints
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Commentary on“International norms for adult handgrip strength:A systematic review of data on 2.4 million adults aged 20 to 100+years from 69 countries and regions”
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作者 Barbara E.Ainsworth Zhining Zhou 《Journal of Sport and Health Science》 2025年第4期40-42,共3页
In the December 2025 issue of the Journal of Sport and Health Science,Tomkinson et al.^(1)present international norms for adult handgrip strength(HGS)developed from a systematic review of 100 studies with 2.4 million ... In the December 2025 issue of the Journal of Sport and Health Science,Tomkinson et al.^(1)present international norms for adult handgrip strength(HGS)developed from a systematic review of 100 studies with 2.4 million adults aged 20 to 100+years from 69 countries and regions.Twenty-eight interna-tional handGRIP Strength(iGRIPS)researchers contributed to the study. 展开更多
关键词 handgrip strength systematic review ADULTS international norms
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Schweizer-Sklar T-Norm Operators for Picture Fuzzy Hypersoft Sets: Advancing Suistainable Technology in Social Healthy Environments
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作者 Xingsi Xue Himanshu Dhumras +2 位作者 Garima Thakur Rakesh Kumar Bajaj Varun Shukla 《Computers, Materials & Continua》 2025年第7期583-606,共24页
Ensuring a sustainable and eco-friendly environment is essential for promoting a healthy and balanced social life.However,decision-making in such contexts often involves handling vague,imprecise,and uncertain informat... Ensuring a sustainable and eco-friendly environment is essential for promoting a healthy and balanced social life.However,decision-making in such contexts often involves handling vague,imprecise,and uncertain information.To address this challenge,this study presents a novel multi-criteria decision-making(MCDM)approach based on picture fuzzy hypersoft sets(PFHSS),integrating the flexibility of Schweizer-Sklar triangular norm-based aggregation operators.The proposed aggregation mechanisms—weighted average and weighted geometric operators—are formulated using newly defined operational laws under the PFHSS framework and are proven to satisfy essential mathematical properties,such as idempotency,monotonicity,and boundedness.The decision-making model system-atically incorporates both benefit and cost-type criteria,enabling more nuanced evaluations in complex social or environmental decision problems.To enhance interpretability and practical relevance,the study conducts a sensitivity analysis on the Schweizer-Sklar parameter(Δ).The results show that varyingΔaffects the strictness of aggregation,thereby influencing the ranking stability of alternatives.A comparative analysis with existing fuzzy and hypersoft-based MCDM methods confirms the robustness,expressiveness,and adaptability of the proposed approach.Notably,the use of picture fuzzy sets allows for the inclusion of positive,neutral,and negative memberships,offering a richer representation of expert opinions compared to traditional models.A case study focused on green technology adoption for environmental sustainability illustrates the real-world applicability of the proposed method.The analysis confirms that the approach yields consistent and interpretable results,even under varying degrees of decision uncertainty.Overall,this work contributes an efficient and flexible MCDM tool that can support decision-makers in formulating policies aligned with sustainable and socially responsible outcomes. 展开更多
关键词 Hypersoft set picture fuzzy set Schweizer-Sklar norms aggregation operators DECISION-MAKING green technology adoption
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International Norms for Lethal Autonomous Weapon Systems:Divergences among Major Countries
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作者 Chen Genfeng Sun Xuefeng 《Contemporary International Relations》 2025年第2期19-36,共18页
Lethal autonomous weapon systems(LAWS)have become a prominent issue in global security governance.However,significant divergences remain among the world's major countries regarding their regulation:some support re... Lethal autonomous weapon systems(LAWS)have become a prominent issue in global security governance.However,significant divergences remain among the world's major countries regarding their regulation:some support restrictions,while others oppose any binding rules,and there are also countries adopting a neutral stance,though their choices are subject to external pressure.In the foreseeable future,the deployment of LAWS will increase further,raising the risk of arms races,with international support for regulating these weapons gaining strength.Yet,establishing legally binding international rules within the United Nations(UN)framework remains a distant prospect.Given this backdrop,China should continue to actively participate in international norms setting concerning LAWS under UN auspices and deepen coordination and cooperation with other countries towards more applicable norms and global governance frameworks. 展开更多
关键词 international norm international security lethal autonomous weapon system major country
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Table manners
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作者 Maria Chiu 《空中英语教室(初级版.大家说英语)》 2025年第10期21-24,52,53,56,共7页
Table manners are important in manycultures.Inmost countries,youwait untileveryone has food before eating.Youalso don't put your elbows on the table.It's not polite to eat too fast.And youshouldn't talk wi... Table manners are important in manycultures.Inmost countries,youwait untileveryone has food before eating.Youalso don't put your elbows on the table.It's not polite to eat too fast.And youshouldn't talk with food in yourmouth orchew with your mouth open. 展开更多
关键词 eating etiquette cultural norms table manners
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Qualitative systematic review of the socioeconomic factors affecting type 2 diabetes management in Pakistan
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作者 Affan Faisal Muhammad Awais +5 位作者 Zain Tariq Abdul Basit Tahleel Abbas Fnu Farzeela Abdullah Iftikhar Abdul M Basil 《World Journal of Diabetes》 2025年第10期363-369,共7页
BACKGROUND Type 2 diabetes mellitus(T2DM)is increasing rapidly in Pakistan,especially among socioeconomically disadvantaged populations.While clinical care remains central,social determinants such as poverty,gender no... BACKGROUND Type 2 diabetes mellitus(T2DM)is increasing rapidly in Pakistan,especially among socioeconomically disadvantaged populations.While clinical care remains central,social determinants such as poverty,gender norms,and mistrust in healthcare critically shape disease outcomes.AIM To synthesize qualitative evidence on how these factors influence the experience and management of T2DM in Pakistan.METHODS Following PRISMA guidelines,a systematic review of qualitative studies published between 2000 and 2025 was conducted on February 25,2025 using PubMed,CINAHL,MEDLINE Plus,and PakMediNet.Eleven studies exploring socioeconomic influences on T2DM care and self-management in Pakistan were included.Thematic synthesis was used to identify key patterns.Quality was appraised using the Joanna Briggs Institute Checklist for Qualitative Research.RESULTS Three major themes were identified:(1)Economic insecurity.High cost of treatment,poor rural infrastructure,and food insecurity hinder access and adherence;(2)Sociocultural and gender norms.Restricted mobility of females,family control over health decisions,and fatalistic beliefs delay care;and(3)Knowledge gaps and mistrust.A lack of culturally appropriate education,reliance on traditional remedies,and distrust in public health systems reduce compliance.These intersecting barriers collectively impede effective diabetes management.CONCLUSION T2DM in Pakistan is driven by entrenched social and economic barriers.Addressing it requires culturally sensitive,equity-oriented strategies that go beyond biomedical models.Policy reforms should focus on affordability,rural outreach,and inclusive health education.Future research should engage marginalized voices through participatory methods. 展开更多
关键词 Type 2 diabetes mellitus Socioeconomic determinants Gender norms Health-seeking behavior Health equity Cultural barriers Health literacy
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A Total Variational Image Denoising Model Coupled with a New Non-local Edge Extractor and a Level Set Curvature Gradient
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作者 Zhou Han Xianchun Zhou +1 位作者 Binxin Tang Siqi Lu 《Instrumentation》 2025年第2期47-59,共13页
To solve the problem of false edges in a flat region of l_(1)norm total variational TV model,an edge extractor based on non-local idea is proposed in this paper.The new edge extractor can effectively suppress the infl... To solve the problem of false edges in a flat region of l_(1)norm total variational TV model,an edge extractor based on non-local idea is proposed in this paper.The new edge extractor can effectively suppress the influence of noise and extract the edge information of the image.The new edge extractor is used as the adaptive function and the weighting function of the l_(p) norm variational model to control the noise reduction ability of the model,and a new model 1 is obtained.Considering that the new model 1 only uses the gradient mode as the image feature operator,which is insufficient to express the image texture information,a new level set curvature gradient variational model 2 combined with the edge extractor is proposed.The new model 2 uses the idea of minimum curvature of the level set of clear images to obtain noise reduction images.By coupling new model 1 and new model 2 to smooth the noise and protect more textures,a new Non-local level set denoising model(NLSDM)for image noise reduction is obtained.The experimental results show that compared with the noise reduction model,the new model has significantly improved the peak signal-to-noise ratio and structural similarity,and the effect of noise reduction and edge preservation is better. 展开更多
关键词 TV model the l_(p)norm NONLOCAL pfunction level set curvature term of regularization
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Sobolev space norm regularized full waveform inversion for ultrasound computed tomography
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作者 Panpan Li Yubing Li +2 位作者 Chang Su Zeyuan Dong Weijun Lin 《Chinese Physics B》 2025年第5期444-456,共13页
Full waveform inversion(FWI)is a complex data fitting process based on full wavefield modeling,aiming to quantitatively reconstruct unknown model parameters from partial waveform data with high-resolution.However,this... Full waveform inversion(FWI)is a complex data fitting process based on full wavefield modeling,aiming to quantitatively reconstruct unknown model parameters from partial waveform data with high-resolution.However,this process is highly nonlinear and ill-posed,therefore achieving high-resolution imaging of complex biological tissues within a limited number of iterations remains challenging.We propose a multiscale frequency–domain full waveform inversion(FDFWI)framework for ultrasound computed tomography(USCT)imaging of biological tissues,which innovatively incorporates Sobolev space norm regularization for enhancement of prior information.Specifically,we investigate the effect of different types of hyperparameter on the imaging quality,during which the regularization weight is dynamically adapted based on the ratio of the regularization term to the data fidelity term.This strategy reduces reliance on predefined hyperparameters,ensuring robust inversion performance.The inversion results from both numerical and experimental tests(i.e.,numerical breast,thigh,and ex vivo pork-belly tissue)demonstrate the effectiveness of our regularized FWI strategy.These findings will contribute to the application of the FWI technique in quantitative imaging based on USCT and make USCT possible to be another high-resolution imaging method after x-ray computed tomography and magnetic resonance imaging. 展开更多
关键词 full waveform inversion Sobolev space norm regularization ultrasound computed tomography
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APPLICATION OF THE MINIMAL NORM TENSOR PRINCIPLE IN MOBIUS GEOMETRY
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作者 XING Jin-xiong GONG Yi-fan 《数学杂志》 2025年第3期205-212,共8页
This paper addresses Pinching problems in Möbius geometry for hypersurfaces with Möbius isotropy in the unit sphere.By implementing the minimum norm tensor principle,we rigorously estimate the squared norm o... This paper addresses Pinching problems in Möbius geometry for hypersurfaces with Möbius isotropy in the unit sphere.By implementing the minimum norm tensor principle,we rigorously estimate the squared norm of the quadratic gradient term associated with the Möbius second fundamental form.This analysis yields a critical inequality governing the geometric config-uration.Leveraging this inequality,we subsequently prove a Pinching theorem characterizing the eigenvalues of the Blaschke tensor. 展开更多
关键词 minimal norm tensor Möbius second fundamental form HYPERSURFACE
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我国部分地区居室氡浓度水平调查研究 被引量:37
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作者 王春红 潘自强 +12 位作者 刘森林 杨明理 尚兵 卓维海 任天山 肖德涛 杨维耿 李福生 周开建 吴锦海 宣志强 涂彧 葛良全 《辐射防护》 CAS CSCD 北大核心 2014年第2期65-73,共9页
介绍了2006-2010年我国部分地区居室氡浓度调查的方法和结果。本次调查采用固体核径迹探测器,基本上3个月为一个测量周期,分4个季节进行探测器的布放与回收,多数地区进行了为期1年的测量。共有1个省和7个城市参加了此次调查,测量房... 介绍了2006-2010年我国部分地区居室氡浓度调查的方法和结果。本次调查采用固体核径迹探测器,基本上3个月为一个测量周期,分4个季节进行探测器的布放与回收,多数地区进行了为期1年的测量。共有1个省和7个城市参加了此次调查,测量房间数共计2029个,涉及省市人口约占全国总人口的12%。氡浓度范围5.3—183.0Bq·m^-3,按样本量加权均值为(32.6±5.2)Bq·m^-3,按人口加权均值为(30.7±4.3)Bq·m^-3。氡浓度超过100Bq·^-3的房间(36间)约占总调查房间的1.8%。本次调查结果与20世纪八、九十年代测量结果的比值范围为1.00—3.12,按各城市人口加权均值为1.80。同时对调查地区城镇/农村、不同建筑年代、不同楼层、不同建筑类型、不同地面和墙体材料居室内氡浓度进行了比较分析,分析了调查地区居室内氡浓度季节变化。 展开更多
关键词 室内 调查 比较分析 NORM
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BIO-NORM与NORM耕地质量评价方法对比研究 被引量:22
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作者 于东升 张广星 +3 位作者 张忠启 王兴祥 史学正 杨浩 《土壤学报》 CAS CSCD 北大核心 2011年第2期238-245,共8页
耕地质量评价对指导农业生产具有极为重要的意义,耕地质量评价方法的优劣直接影响到耕地质量评价结果的可靠性。本研究以江西省余江县为研究区域,通过实际调查和样品采集与分析,获取了249个水田表层土壤属性数据和95个田块的水稻产量数... 耕地质量评价对指导农业生产具有极为重要的意义,耕地质量评价方法的优劣直接影响到耕地质量评价结果的可靠性。本研究以江西省余江县为研究区域,通过实际调查和样品采集与分析,获取了249个水田表层土壤属性数据和95个田块的水稻产量数据。利用Norm值法和新建立的Bio-Norm法,分别确定耕地质量评价指标、指标权重和隶属度函数,并对研究区水田耕地质量进行评价。两种评价方法的对比分析表明,Bio-Norm法选取的指标比Norm值法更具有代表性,得出的指标权重更能准确反映指标的重要性,确定的隶属度函数临界值更能反映水稻生长的实际要求。评价结果显示,利用Bio-Norm法得出的耕地质量综合指数(IFI值)与水稻产量回归方程的决定系数(R2)远高于Norm值法,且IFI值与水稻产量各等级的区域分布特征更为吻合。Bio-Norm法评价耕地质量更为科学合理。 展开更多
关键词 耕地质量评价 Norm值 水稻产量 土壤质量
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qRT-PCR分析鳜鱼内参基因的筛选 被引量:15
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作者 李迪 吴萍 +3 位作者 何美凤 李伟 肖调义 褚武英 《生命科学研究》 CAS CSCD 2016年第3期214-217,共4页
选择合适的内参基因是提高实时荧光定量PCR分析(quantitative real-time polymerase chain reaction,qRT-PCR)准确性的首要条件。本实验采取鳜鱼8个不同胚胎发育阶段、5个胚后发育时期和8个成鱼组织为研究对象,应用qRT-PCR技术,分析了RP... 选择合适的内参基因是提高实时荧光定量PCR分析(quantitative real-time polymerase chain reaction,qRT-PCR)准确性的首要条件。本实验采取鳜鱼8个不同胚胎发育阶段、5个胚后发育时期和8个成鱼组织为研究对象,应用qRT-PCR技术,分析了RPL13、RPL19、EF1a、RPL13a、B2M、hprt1和rps29七个内参基因的表达稳定情况。经GeNorm软件分析发现,B2M和RPL13a在鳜鱼成鱼不同组织中表达最稳定;在胚后不同时期中表达最稳定的是EF1a和RPL13a;EF1a和B2M是在不同胚胎发育阶段中表达最稳定的两个基因。根据内参基因标准化因子的配对差异分析V_(n/n+1),在鳜鱼不同组织和不同发育阶段中,均使用两个最稳定表达的内参基因即可达到准确校正的目的。因此,该实验结果为鳜鱼基因表达分析时内参基因的选择提供了参考。 展开更多
关键词 鳜鱼 内参基因 基因筛选 实时荧光定量 PCR Ge NORM 软件
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葡萄实时定量PCR中稳定内参基因的筛选 被引量:9
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作者 查倩 奚晓军 +2 位作者 蒋爱丽 田益华 王世平 《果树学报》 CAS CSCD 北大核心 2016年第3期268-274,共7页
【目的】通过ge Norm软件筛选实时荧光定量PCR稳定内参基因,基于多内参基因评价体系进行目的基因相对表达量的计算。【方法】获得不同葡萄品种叶片和果皮中6个候选内参基因的循环阈值(Ct),利用ge Norm软件计算内参基因平均表达稳定性数... 【目的】通过ge Norm软件筛选实时荧光定量PCR稳定内参基因,基于多内参基因评价体系进行目的基因相对表达量的计算。【方法】获得不同葡萄品种叶片和果皮中6个候选内参基因的循环阈值(Ct),利用ge Norm软件计算内参基因平均表达稳定性数值M和基因配对差异值V,从而判断内参基因最适组合。【结果】发现候选内参基因表达稳定性由高到低的排列顺序为Vv EF1r=Vv EF1-α>Vv GAPDH>Vv ACTIN>Vv ACT1>Vv UBQ,不同组织分别分析均发现Vv EF1-α和Vv EF1r的稳定性最高,且基因配对差异值V2/3为0.104,所以内参基因的最适组合为Vv EF1-α和Vv EF1r。利用ge Norm软件计算得到的多内参基因评价体系的标准化因子可应用于目的基因的相对表达量分析。【结论】ge Norm软件筛选实时荧光定量PCR稳定内参基因的方法可以应用到多条件和多组织的不同植物样本中,并用于目的基因表达谱的研究,具有重要的广泛应用价值。 展开更多
关键词 葡萄 ge NORM 内参基因 标准化因子 实时荧光定量PCR
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