Modulated electro-hyperthermia (mEHT) targets tissue’s natural electric and thermal heterogeneities to heat the cancer cells selectively. The applied 13.56 MHz radiofrequency (RF) is a carrier of the low-frequency mo...Modulated electro-hyperthermia (mEHT) targets tissue’s natural electric and thermal heterogeneities to heat the cancer cells selectively. The applied 13.56 MHz radiofrequency (RF) is a carrier of the low-frequency modulation. The high-frequency part was chosen to select the malignant lesion using the specialties of the tumor: the higher conductivity and dielectric constant of the tumor than its host. The electric field selects the tumor, and the low-frequency amplitude modulation polarizes and excites the transmembrane proteins of the malignant cells. The dominant absorption of the energy by the microscopic clusters of the membrane rafts acts like nanoparticle heating. Exciting the membrane produces various apoptotic signals. The processes were modeled using silico and phantom experiments, which proved the concept. The preclinical verification was made in vitro and in vivo, and in the end, clinical proofs validated the method. Our objective is to follow all the development steps from the laboratory to the clinics in a trilogy of articles. This present is the first part, which deals with in silico, phantom, and in vitro research.展开更多
Costus pictus D.,a medicinal herb renowned for its antidiabetic and antioxidant potential,remains underutilized due to its high perishability and the lack of effective post-harvest preservation technologies.This study...Costus pictus D.,a medicinal herb renowned for its antidiabetic and antioxidant potential,remains underutilized due to its high perishability and the lack of effective post-harvest preservation technologies.This study used ultraviolet-C(UV-C)radiation(254 nm,10 min,8 W;228.6 mJ/cm^(2))as a novel,non-thermal pretreatment to enhance drying efficiency and bioactive compound retention in C.pictus leaves.Post UV-C exposure,the leaves were subjected to three conventional drying techniques,such as hot air drying(HD),vacuum drying(VD),and freeze drying(FD),at temperatures of 40◦C,50◦C,and 60◦C.Drying behavior was analyzed through empirical kinetic modelling using six thin-layer models,with the Page model demonstrating superior predictive accuracy(R2=0.998).UV-C pre-treatment significantly enhanced effective moisture diffusivity,reduced activation energy requirements,and improved mass transfer coefficients,accelerating the drying process.Drying durations were notably shortened across all methods,for example,in HD,from 120-300 min to 90-120 min,and in VD,from 360-540 min to 300-360 min.Regarding phytochemical preservation,vacuum-dried UV-C pretreated samples exhibited the highest retention of total phenolics and flavonoids,while freeze-dried counterparts demonstrated superior antioxidant capacity and microstructural integrity.Scanning electron microscopy(SEM)revealed microstructural modifications induced by UV-C treatment that facilitated enhanced moisture migration.FTIR spectroscopy confirmed the preservation of key functional groups associated with antioxidant bioactivity.These findings support the potential of UV-C-assisted drying as a scalable,energy-efficient technique for valorizing C.pictus in functional food and nutraceutical applications targeting chronic disease prevention and management.展开更多
文摘Modulated electro-hyperthermia (mEHT) targets tissue’s natural electric and thermal heterogeneities to heat the cancer cells selectively. The applied 13.56 MHz radiofrequency (RF) is a carrier of the low-frequency modulation. The high-frequency part was chosen to select the malignant lesion using the specialties of the tumor: the higher conductivity and dielectric constant of the tumor than its host. The electric field selects the tumor, and the low-frequency amplitude modulation polarizes and excites the transmembrane proteins of the malignant cells. The dominant absorption of the energy by the microscopic clusters of the membrane rafts acts like nanoparticle heating. Exciting the membrane produces various apoptotic signals. The processes were modeled using silico and phantom experiments, which proved the concept. The preclinical verification was made in vitro and in vivo, and in the end, clinical proofs validated the method. Our objective is to follow all the development steps from the laboratory to the clinics in a trilogy of articles. This present is the first part, which deals with in silico, phantom, and in vitro research.
文摘Costus pictus D.,a medicinal herb renowned for its antidiabetic and antioxidant potential,remains underutilized due to its high perishability and the lack of effective post-harvest preservation technologies.This study used ultraviolet-C(UV-C)radiation(254 nm,10 min,8 W;228.6 mJ/cm^(2))as a novel,non-thermal pretreatment to enhance drying efficiency and bioactive compound retention in C.pictus leaves.Post UV-C exposure,the leaves were subjected to three conventional drying techniques,such as hot air drying(HD),vacuum drying(VD),and freeze drying(FD),at temperatures of 40◦C,50◦C,and 60◦C.Drying behavior was analyzed through empirical kinetic modelling using six thin-layer models,with the Page model demonstrating superior predictive accuracy(R2=0.998).UV-C pre-treatment significantly enhanced effective moisture diffusivity,reduced activation energy requirements,and improved mass transfer coefficients,accelerating the drying process.Drying durations were notably shortened across all methods,for example,in HD,from 120-300 min to 90-120 min,and in VD,from 360-540 min to 300-360 min.Regarding phytochemical preservation,vacuum-dried UV-C pretreated samples exhibited the highest retention of total phenolics and flavonoids,while freeze-dried counterparts demonstrated superior antioxidant capacity and microstructural integrity.Scanning electron microscopy(SEM)revealed microstructural modifications induced by UV-C treatment that facilitated enhanced moisture migration.FTIR spectroscopy confirmed the preservation of key functional groups associated with antioxidant bioactivity.These findings support the potential of UV-C-assisted drying as a scalable,energy-efficient technique for valorizing C.pictus in functional food and nutraceutical applications targeting chronic disease prevention and management.
