Hemoglobin is a vital protein in red blood cells responsible for transporting oxygen throughout the body.Its accurate measurement is crucial for diagnosing and managing conditions such as anemia and diabetes,where abn...Hemoglobin is a vital protein in red blood cells responsible for transporting oxygen throughout the body.Its accurate measurement is crucial for diagnosing and managing conditions such as anemia and diabetes,where abnormal hemoglobin levels can indicate significant health issues.Traditional methods for hemoglobin measurement are invasive,causing pain,risk of infection,and are less convenient for frequent monitoring.PPG is a transformative technology in wearable healthcare for noninvasive monitoring and widely explored for blood pressure,sleep,blood glucose,and stress analysis.In this work,we propose a hemoglobin estimation method using an adaptive lightweight convolutional neural network(HMALCNN)from PPG.The HMALCNN is designed to capture both fine-grained local waveform characteristics and global contextual patterns,ensuring robust performance across acquisition settings.We validated our approach on two multi-regional datasets containing 152 and 68 subjects,respectively,employing a subjectindependent 5-fold cross-validation strategy.The proposed method achieved root mean square errors(RMSE)of 0.90 and 1.20 g/dL for the two datasets,with strong Pearson correlations of 0.82 and 0.72.We conducted extensive posthoc analyses to assess clinical utility and interpretability.A±1 g/dL clinical error tolerance evaluation revealed that 91.3%and 86.7%of predictions for the two datasets fell within the acceptable clinical range.Hemoglobin range-wise analysis demonstrated consistently high accuracy in the normal and low hemoglobin categories.Statistical significance testing using the Wilcoxon signed-rank test confirmed the stability of performance across validation folds(p>0.05 for both RMSE and correlation).Furthermore,model interpretability was enhanced using Gradient-weighted Class Activation Mapping(Grad-CAM),supporting the model’s clinical trustworthiness.The proposed HMALCNN offers a computationally efficient,clinically interpretable,and generalizable framework for noninvasive hemoglobin monitoring,with strong potential for integration into wearable healthcare systems as a practical alternative to invasive measurement techniques.展开更多
In the last two decades,advances in immunosuppressive regimens have led to fewer complications of acute rejection crisis and consequently improved shortterm graft and patient survival.In parallel with this great succe...In the last two decades,advances in immunosuppressive regimens have led to fewer complications of acute rejection crisis and consequently improved shortterm graft and patient survival.In parallel with this great success,long-term posttransplantation complications have become a focus of interest of doctors engaged in transplant medicine.Metabolic syndrome(MetS)and its individual components,namely,obesity,dyslipidemia,diabetes,and hypertension,often develop in the post-transplant setting and are associated with immunosuppressive therapy.Nonalcoholic fatty liver disease(NAFLD)is closely related to MetS and its individual components and is the liver manifestation of MetS.Therefore,it is not surprising that MetS and its individual components are associated with recurrent or“de novo”NAFLD after liver transplantation(LT).Fibrosis of the graft is one of the main determinants of overall morbidity and mortality in the post-LT period.In the assessment of post-LT steatosis and fibrosis,we have biochemical markers,imaging methods and liver biopsy.Because of the significant economic burden of post-LT steatosis and fibrosis and its potential consequences,there is an unmet need for noninvasive methods that are efficient and cost-effective.Biochemical scores can overestimate fibrosis and are not a good method for fibrosis evaluation in liver transplant recipients due to frequent post-LT thrombocytopenia.Transient elastography with controlled attenuation parameter is a promising noninvasive method for steatosis and fibrosis.In this review,we will specifically focus on the evaluation of steatosis and fibrosis in the post-LT setting in the context of de novo or recurrent NAFLD.展开更多
Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted supply.Raman spectroscopy performances high specificity on HSA detection and it is even possi...Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted supply.Raman spectroscopy performances high specificity on HSA detection and it is even possible to determine HSA injectable products noninvasively.In this study,we developed a noninvasive rapid screening method for of HSA injectable products by using portable Raman spectrometer.Qualitative models were established by using principal component analysis combined with classical least squares(PCA-CLS)algorithm,while quanti-tative model was established by using partial least squares(PLS)algorithm.Model transfer in different instruments of both the same and different apparatus modules was further discussed in this paper.A total of 34 HSA injectable samples collected from markets were used for verification.The identification results showed 100%accuracy and the predicted concentrations of those identified as true HSA were consistent with their labeled concentrations.The quantitative results also indicated that model transfer was excellent in the same apparatus modules of Raman spectrometer at all concentration levels,and still good enough in the different apparatus modules although the relative standard deviation(RSD)value showed a little increasing trend at low HSA concentration level.In conclusion,the method was proved to be feasible and efficient for screening HSA injections,especially on its screening speed and the consideration of glass containers.Moreover,with inspiring results on the model transfer,the method could be used as a universal screening mean to different Raman instruments.展开更多
Microcracks are common in compact bone,but their continued propagation can lead to macroscopic fractures.These microcracks cannot be visualized radiographically,necessitating alternative noninvasive methods to identif...Microcracks are common in compact bone,but their continued propagation can lead to macroscopic fractures.These microcracks cannot be visualized radiographically,necessitating alternative noninvasive methods to identify excessive microcracking and prevent fractures.In this study,terahertz time-domain spectroscopy(THz-TDS)was used to examine bone interiors near cracks resulting from loading in bovine tibia samples.Various loading configurations,such as impact,quasi-static loading,and fatigue loading,known to induce different types of micro-scale damage,were applied.The values of refractive index and absorption coefficient of the bone samples were then determined from the THz-TDS spectra acquired before loading and after fracture.The study revealed that different loading configurations led to varying terahertz optical coefficients associated with various types of bone fractures.Specifically,the refractive index notably increased under fatigue loading but remained relatively stable during quasi-static bending.The absorption coefficient of bone decreased only under fatigue loading.Furthermore,samples were subjected to axial and radial impacts without sustaining damage.Results indicated that in the undamaged state,the change in refractive index was smaller compared to after impact failure,while the change in absorption coefficient remained consistent after failure.Under radial impact loading,changes in refractive index and absorption coefficient were significantly more pronounced than under axial loading.Prior to loading,the measured value of refractive index was 2.72±0.11,and the absorption coefficient was 6.33±0.09 mm^(−1)at 0.5 THz.展开更多
Objective and Impact Statement:High-intensity focused ultrasound(HIFU)therapy is a promising noninvasive method that induces coagulative necrosis in diseased tissues through thermal and cavitation effects,while avoidi...Objective and Impact Statement:High-intensity focused ultrasound(HIFU)therapy is a promising noninvasive method that induces coagulative necrosis in diseased tissues through thermal and cavitation effects,while avoiding surrounding damage to surrounding normal tissues.Introduction:Accurate and real-time acquisition of the focal region temperature field during HIFU treatment marked enhances therapeutic efficacy,holding paramount scientific and practical value in clinical cancer therapy.Methods:In this paper,we initially designed and assembled an integrated HIFU system incorporating diagnostic,therapeutic,and temperature measurement functionalities to collect ultrasound echo signals and temperature variations during HIFU therapy.Furthermore,we introduced a novel multimodal teacher-student model approach,which utilizes the shared self-expressive coefficients and the deep canonical correlation analysis layer to aggregate each modality data,then through knowledge distillation strategies,transfers the knowledge from the teacher model to the student model.Results:By investigating the relationship between the phantoms,in vitro,and in vivo ultrasound echo signals and temperatures,we successfully achieved real-time reconstruction of the HIFU focal 2D temperature field region with a maximum temperature error of less than 2.5℃.Conclusion:Our method effectively monitored the distribution of the HIFU temperature field in real time,providing scientifically precise predictive schemes for HIFU therapy,laying a theoretical foundation for subsequent personalized treatment dose planning,and providing efficient guidance for noninvasive,nonionizing cancer treatment.展开更多
Liver biopsy (LB) has traditionally been considered the gold standard for pretreatment evaluation of liver fibrosis in patients with chronic hepatitis C (CHC). However, LB is an invasive procedure with several shortco...Liver biopsy (LB) has traditionally been considered the gold standard for pretreatment evaluation of liver fibrosis in patients with chronic hepatitis C (CHC). However, LB is an invasive procedure with several shortcomings (intra-and interobserver variability of histopathological interpretation, sampling errors, high cost) and the risk of rare but potentially life-threatening complications. In addition, LB is poorly accepted by patients and it is not suitable for repeated evaluation. Further-more, the prevalence of CHC makes LB unrealistic to be performed in all patients with this disease who are candidates for antiviral therapy. The above-mentioned drawbacks of LB have led to the development of non-invasive methods for the assessment of liver fibrosis. Several noninvasive methods, ranging from serum marker assays to advanced imaging techniques, have proved to be excellent tools for the evaluation of liver fibrosis in patients with CHC, whereas the value of LB as a gold standard for staging fibrosis prior to antiviral therapy has become questionable for clinicians. Despite significant resistance from those in favor of LB, noninvasive methods for pretreatment assessment of liver fibrosis in patients with CHC have become part of routine clinical practice. With protease inhibitors-based triple therapy already available and substantial improvement in sustained virological response, the time has come to move forward to noninvasiveness, with no risks for the patient and, thus, no need for LB in the assessment of liver fibrosis in the decision making for antiviral therapy in CHC.展开更多
Ultrasound(US)has emerged as a noninvasive neurostimulation method for motor control in Parkinson’s disease(PD).Previous in vivo US neuromodulation studies for PD were single-target stimulation.However,the motor symp...Ultrasound(US)has emerged as a noninvasive neurostimulation method for motor control in Parkinson’s disease(PD).Previous in vivo US neuromodulation studies for PD were single-target stimulation.However,the motor symptoms of PD are linked with neural circuit dysfunction,and multi-target stimulation is conducted in clinical treatment for PD.Thus,in the present study,we achieved multi-target US stimulation using holographic lens transducer based on the Rayleigh–Sommerfeld diffraction integral and time-reversal methods.We demonstrated that holographic US stimulation of the bilateral dorsal striatum(DS)could improve the motor function in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced mouse model of PD.The holographic US wave(fundamental frequency:3 MHz,pulse repetition frequency:500 Hz,duty cycle:20%,tone-burst duration:0.4 ms,sonication duration:1 s,interstimulus interval:4 s,spatial-peak temporal-average intensity:180 mw/cm2)was delivered to the bilateral DS 20 min per day for consecutive 10 d after the last injection of MPTP.Immunohistochemical c-Fos staining demonstrated that holographic US significantly increased the c-Fos-positive neurons in the bilateral DS compared with the sham group(P=0.003).Moreover,our results suggested that holographic US stimulation of the bilateral DS ameliorated motor dysfunction(P<0.05)and protected the dopaminergic(DA)neurons(P<0.001).The neuroprotective effect of holographic US was associated with the prevention of axon degeneration and the reinforcement of postsynaptic densities[growth associated protein-43(P<0.001),phosphorylated Akt(P=0.001),β3-tubulin(P<0.001),phosphorylated CRMP2(P=0.037),postsynaptic density(P=0.023)].These data suggested that holographic US-induced acoustic radiation force has the potential to achieve multi-target neuromodulation and could serve as a reliable tool for the treatment of PD.展开更多
基金funded by the Deanship of Graduate Studies and Scientific Research at Qassim University for financial support(QU-APC-2025).
文摘Hemoglobin is a vital protein in red blood cells responsible for transporting oxygen throughout the body.Its accurate measurement is crucial for diagnosing and managing conditions such as anemia and diabetes,where abnormal hemoglobin levels can indicate significant health issues.Traditional methods for hemoglobin measurement are invasive,causing pain,risk of infection,and are less convenient for frequent monitoring.PPG is a transformative technology in wearable healthcare for noninvasive monitoring and widely explored for blood pressure,sleep,blood glucose,and stress analysis.In this work,we propose a hemoglobin estimation method using an adaptive lightweight convolutional neural network(HMALCNN)from PPG.The HMALCNN is designed to capture both fine-grained local waveform characteristics and global contextual patterns,ensuring robust performance across acquisition settings.We validated our approach on two multi-regional datasets containing 152 and 68 subjects,respectively,employing a subjectindependent 5-fold cross-validation strategy.The proposed method achieved root mean square errors(RMSE)of 0.90 and 1.20 g/dL for the two datasets,with strong Pearson correlations of 0.82 and 0.72.We conducted extensive posthoc analyses to assess clinical utility and interpretability.A±1 g/dL clinical error tolerance evaluation revealed that 91.3%and 86.7%of predictions for the two datasets fell within the acceptable clinical range.Hemoglobin range-wise analysis demonstrated consistently high accuracy in the normal and low hemoglobin categories.Statistical significance testing using the Wilcoxon signed-rank test confirmed the stability of performance across validation folds(p>0.05 for both RMSE and correlation).Furthermore,model interpretability was enhanced using Gradient-weighted Class Activation Mapping(Grad-CAM),supporting the model’s clinical trustworthiness.The proposed HMALCNN offers a computationally efficient,clinically interpretable,and generalizable framework for noninvasive hemoglobin monitoring,with strong potential for integration into wearable healthcare systems as a practical alternative to invasive measurement techniques.
文摘In the last two decades,advances in immunosuppressive regimens have led to fewer complications of acute rejection crisis and consequently improved shortterm graft and patient survival.In parallel with this great success,long-term posttransplantation complications have become a focus of interest of doctors engaged in transplant medicine.Metabolic syndrome(MetS)and its individual components,namely,obesity,dyslipidemia,diabetes,and hypertension,often develop in the post-transplant setting and are associated with immunosuppressive therapy.Nonalcoholic fatty liver disease(NAFLD)is closely related to MetS and its individual components and is the liver manifestation of MetS.Therefore,it is not surprising that MetS and its individual components are associated with recurrent or“de novo”NAFLD after liver transplantation(LT).Fibrosis of the graft is one of the main determinants of overall morbidity and mortality in the post-LT period.In the assessment of post-LT steatosis and fibrosis,we have biochemical markers,imaging methods and liver biopsy.Because of the significant economic burden of post-LT steatosis and fibrosis and its potential consequences,there is an unmet need for noninvasive methods that are efficient and cost-effective.Biochemical scores can overestimate fibrosis and are not a good method for fibrosis evaluation in liver transplant recipients due to frequent post-LT thrombocytopenia.Transient elastography with controlled attenuation parameter is a promising noninvasive method for steatosis and fibrosis.In this review,we will specifically focus on the evaluation of steatosis and fibrosis in the post-LT setting in the context of de novo or recurrent NAFLD.
基金Youth Develop-ment Research Foundation(No.2015C03)of Na-tional Institutes of Food and Drug Control,P.R.China.
文摘Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted supply.Raman spectroscopy performances high specificity on HSA detection and it is even possible to determine HSA injectable products noninvasively.In this study,we developed a noninvasive rapid screening method for of HSA injectable products by using portable Raman spectrometer.Qualitative models were established by using principal component analysis combined with classical least squares(PCA-CLS)algorithm,while quanti-tative model was established by using partial least squares(PLS)algorithm.Model transfer in different instruments of both the same and different apparatus modules was further discussed in this paper.A total of 34 HSA injectable samples collected from markets were used for verification.The identification results showed 100%accuracy and the predicted concentrations of those identified as true HSA were consistent with their labeled concentrations.The quantitative results also indicated that model transfer was excellent in the same apparatus modules of Raman spectrometer at all concentration levels,and still good enough in the different apparatus modules although the relative standard deviation(RSD)value showed a little increasing trend at low HSA concentration level.In conclusion,the method was proved to be feasible and efficient for screening HSA injections,especially on its screening speed and the consideration of glass containers.Moreover,with inspiring results on the model transfer,the method could be used as a universal screening mean to different Raman instruments.
基金supported by the National Natural Science Foundation of China(Grant Nos.11972247 and 12372080).
文摘Microcracks are common in compact bone,but their continued propagation can lead to macroscopic fractures.These microcracks cannot be visualized radiographically,necessitating alternative noninvasive methods to identify excessive microcracking and prevent fractures.In this study,terahertz time-domain spectroscopy(THz-TDS)was used to examine bone interiors near cracks resulting from loading in bovine tibia samples.Various loading configurations,such as impact,quasi-static loading,and fatigue loading,known to induce different types of micro-scale damage,were applied.The values of refractive index and absorption coefficient of the bone samples were then determined from the THz-TDS spectra acquired before loading and after fracture.The study revealed that different loading configurations led to varying terahertz optical coefficients associated with various types of bone fractures.Specifically,the refractive index notably increased under fatigue loading but remained relatively stable during quasi-static bending.The absorption coefficient of bone decreased only under fatigue loading.Furthermore,samples were subjected to axial and radial impacts without sustaining damage.Results indicated that in the undamaged state,the change in refractive index was smaller compared to after impact failure,while the change in absorption coefficient remained consistent after failure.Under radial impact loading,changes in refractive index and absorption coefficient were significantly more pronounced than under axial loading.Prior to loading,the measured value of refractive index was 2.72±0.11,and the absorption coefficient was 6.33±0.09 mm^(−1)at 0.5 THz.
基金supported in part by the Natural Science Foundation of China(No.U22A20259)the Shenzhen Basic Science Research(No.JCYJ20200109110006136)the Interdisciplinary Research Program of Hust 2023JCYJ043.
文摘Objective and Impact Statement:High-intensity focused ultrasound(HIFU)therapy is a promising noninvasive method that induces coagulative necrosis in diseased tissues through thermal and cavitation effects,while avoiding surrounding damage to surrounding normal tissues.Introduction:Accurate and real-time acquisition of the focal region temperature field during HIFU treatment marked enhances therapeutic efficacy,holding paramount scientific and practical value in clinical cancer therapy.Methods:In this paper,we initially designed and assembled an integrated HIFU system incorporating diagnostic,therapeutic,and temperature measurement functionalities to collect ultrasound echo signals and temperature variations during HIFU therapy.Furthermore,we introduced a novel multimodal teacher-student model approach,which utilizes the shared self-expressive coefficients and the deep canonical correlation analysis layer to aggregate each modality data,then through knowledge distillation strategies,transfers the knowledge from the teacher model to the student model.Results:By investigating the relationship between the phantoms,in vitro,and in vivo ultrasound echo signals and temperatures,we successfully achieved real-time reconstruction of the HIFU focal 2D temperature field region with a maximum temperature error of less than 2.5℃.Conclusion:Our method effectively monitored the distribution of the HIFU temperature field in real time,providing scientifically precise predictive schemes for HIFU therapy,laying a theoretical foundation for subsequent personalized treatment dose planning,and providing efficient guidance for noninvasive,nonionizing cancer treatment.
文摘Liver biopsy (LB) has traditionally been considered the gold standard for pretreatment evaluation of liver fibrosis in patients with chronic hepatitis C (CHC). However, LB is an invasive procedure with several shortcomings (intra-and interobserver variability of histopathological interpretation, sampling errors, high cost) and the risk of rare but potentially life-threatening complications. In addition, LB is poorly accepted by patients and it is not suitable for repeated evaluation. Further-more, the prevalence of CHC makes LB unrealistic to be performed in all patients with this disease who are candidates for antiviral therapy. The above-mentioned drawbacks of LB have led to the development of non-invasive methods for the assessment of liver fibrosis. Several noninvasive methods, ranging from serum marker assays to advanced imaging techniques, have proved to be excellent tools for the evaluation of liver fibrosis in patients with CHC, whereas the value of LB as a gold standard for staging fibrosis prior to antiviral therapy has become questionable for clinicians. Despite significant resistance from those in favor of LB, noninvasive methods for pretreatment assessment of liver fibrosis in patients with CHC have become part of routine clinical practice. With protease inhibitors-based triple therapy already available and substantial improvement in sustained virological response, the time has come to move forward to noninvasiveness, with no risks for the patient and, thus, no need for LB in the assessment of liver fibrosis in the decision making for antiviral therapy in CHC.
基金supported,in part,by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0930000)the National Natural Science Foundation of China(nos.T2122023,U23A20479,12227809,and 12104478)+1 种基金the Natural Science Foundation of Guangdong Province(no.202381515040008)Shenzhen Basic Science Research(nos.JCYJ20220818101608018,20231123154444001,and 20220812171109001).
文摘Ultrasound(US)has emerged as a noninvasive neurostimulation method for motor control in Parkinson’s disease(PD).Previous in vivo US neuromodulation studies for PD were single-target stimulation.However,the motor symptoms of PD are linked with neural circuit dysfunction,and multi-target stimulation is conducted in clinical treatment for PD.Thus,in the present study,we achieved multi-target US stimulation using holographic lens transducer based on the Rayleigh–Sommerfeld diffraction integral and time-reversal methods.We demonstrated that holographic US stimulation of the bilateral dorsal striatum(DS)could improve the motor function in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced mouse model of PD.The holographic US wave(fundamental frequency:3 MHz,pulse repetition frequency:500 Hz,duty cycle:20%,tone-burst duration:0.4 ms,sonication duration:1 s,interstimulus interval:4 s,spatial-peak temporal-average intensity:180 mw/cm2)was delivered to the bilateral DS 20 min per day for consecutive 10 d after the last injection of MPTP.Immunohistochemical c-Fos staining demonstrated that holographic US significantly increased the c-Fos-positive neurons in the bilateral DS compared with the sham group(P=0.003).Moreover,our results suggested that holographic US stimulation of the bilateral DS ameliorated motor dysfunction(P<0.05)and protected the dopaminergic(DA)neurons(P<0.001).The neuroprotective effect of holographic US was associated with the prevention of axon degeneration and the reinforcement of postsynaptic densities[growth associated protein-43(P<0.001),phosphorylated Akt(P=0.001),β3-tubulin(P<0.001),phosphorylated CRMP2(P=0.037),postsynaptic density(P=0.023)].These data suggested that holographic US-induced acoustic radiation force has the potential to achieve multi-target neuromodulation and could serve as a reliable tool for the treatment of PD.
