The S-RNase-based self-incompatibility locus(S-locus)in Petunia species contains 16-20 F-box genes,which collaboratively function in the recognition and subsequent degradation of non-self S-RNases,while distinguishing...The S-RNase-based self-incompatibility locus(S-locus)in Petunia species contains 16-20 F-box genes,which collaboratively function in the recognition and subsequent degradation of non-self S-RNases,while distinguishing them from self S-RNase.However,the number of S-locus F-box genes(SFBBs)physically interacted with non-self S-RNases remains uncertain in Pyrus species.Utilizing Pacbio long-read sequencing,we successfully assembled the genome of pear cultivar‘Yali’(Pyrus bretschneideri),and identified 19 SFBBs from the Pyrus S_(17)-locus spanning approximately 1.78 Mb.Additionally,we identified 17-21 SFBBs from other Pyrus and Malus S-loci spanning a range of 1.35 to 2.64 Mb.Based on the phylogenetic analysis,it was determined that Pyrus and Malus SFBBs could be classified into 22 groups,denoted as Ⅰ to ⅩⅫ.At amino acid level,SFBBs within a given group exhibited average identities ranged from 88.9%to 97.9%.Notably,all 19 SFBBs from the S_(17)-locus co-segregated with S_(17)-RNase,with 18 of them being specifically expressed in pollen.Consequently,these 18 pollen-specifically expressed SFBBs are considered potential candidates for the pollen-S determinant.Intriguingly,out of the 18 pollen-specifically expressed SFBBs,eight demonstrated interactions with at least one non-self S-RNase,while the remaining SFBBs failed to recognize any S-RNase.These findings provide compelling evidence supporting the existence of a collaborative non-self-recognition system governing self-incompatibility in pear species.展开更多
基金supported by the National Key Research and Development Program(2022YFD1200501)the National Science Foundation of China(32172511)+1 种基金the Key Research and Development Program(Modern Agriculture)of Jiangsu Province(BE2022381)the Earmarked Fund for the China Agriculture Research System(CARS-28).
文摘The S-RNase-based self-incompatibility locus(S-locus)in Petunia species contains 16-20 F-box genes,which collaboratively function in the recognition and subsequent degradation of non-self S-RNases,while distinguishing them from self S-RNase.However,the number of S-locus F-box genes(SFBBs)physically interacted with non-self S-RNases remains uncertain in Pyrus species.Utilizing Pacbio long-read sequencing,we successfully assembled the genome of pear cultivar‘Yali’(Pyrus bretschneideri),and identified 19 SFBBs from the Pyrus S_(17)-locus spanning approximately 1.78 Mb.Additionally,we identified 17-21 SFBBs from other Pyrus and Malus S-loci spanning a range of 1.35 to 2.64 Mb.Based on the phylogenetic analysis,it was determined that Pyrus and Malus SFBBs could be classified into 22 groups,denoted as Ⅰ to ⅩⅫ.At amino acid level,SFBBs within a given group exhibited average identities ranged from 88.9%to 97.9%.Notably,all 19 SFBBs from the S_(17)-locus co-segregated with S_(17)-RNase,with 18 of them being specifically expressed in pollen.Consequently,these 18 pollen-specifically expressed SFBBs are considered potential candidates for the pollen-S determinant.Intriguingly,out of the 18 pollen-specifically expressed SFBBs,eight demonstrated interactions with at least one non-self S-RNase,while the remaining SFBBs failed to recognize any S-RNase.These findings provide compelling evidence supporting the existence of a collaborative non-self-recognition system governing self-incompatibility in pear species.
