In this paper, we demonstrate a scheme for compensating distorted optical vortex beams carrying orbital angular momentum. By inputting the intensity profile into the Gerchberg–Saxton algorithm [Optik 35, 237(1972)], ...In this paper, we demonstrate a scheme for compensating distorted optical vortex beams carrying orbital angular momentum. By inputting the intensity profile into the Gerchberg–Saxton algorithm [Optik 35, 237(1972)], the pre-compensation phase mask can be acquired. No additional probe beams are introduced, and all the computing is aiming at the transmitted vortex beams. The distorted vortex beams are investigated experimentally before and after pre-compensation, showing favorable compensation performance. This scheme will find applications in the areas of rotation detection, optical communications, and so on.展开更多
A pair of primers created from information of PmNOBⅢ genome DNA Sal I fragment produced a 355bp band by using Penaeus chinensis non occluded baculovirus (PcNOBV),the WSBV isolate from P.hinensis in China's Ma...A pair of primers created from information of PmNOBⅢ genome DNA Sal I fragment produced a 355bp band by using Penaeus chinensis non occluded baculovirus (PcNOBV),the WSBV isolate from P.hinensis in China's Mainland,as the DNA template.The specific PCR product was cloned,sequenced and labeled with digoxigenin (DIG)DNA labeling kit(Boehringer Mannheim).The DIG labeled fragment was tested by dot blot hybridization for sensitivity and specificity with purified PcNOBV nucleocapsid,PcNOBV infected shrimp tissues and healthy shrimp tissues.The detection limit of the DNA probe is 6.8pg of purified PcNOBV DNA.No hybridization signals were observed using DNA from healthy shrimp as template.Healthy P.chinensis,artificially infected P.chinensis and pond reared adult P.chinensis were screened for PcNOBV infection by both PCR and the hybridization assay.The results showed a good relationship between PCR and the hybridization assay.These findings demonstrate that the DIG labeled probe can be used as a sensitive,specific and cost effective reagent for detection of PcNOBV.展开更多
基金National Basic Research Program of China(973 Program)(2014CB340002,2014CB340004)
文摘In this paper, we demonstrate a scheme for compensating distorted optical vortex beams carrying orbital angular momentum. By inputting the intensity profile into the Gerchberg–Saxton algorithm [Optik 35, 237(1972)], the pre-compensation phase mask can be acquired. No additional probe beams are introduced, and all the computing is aiming at the transmitted vortex beams. The distorted vortex beams are investigated experimentally before and after pre-compensation, showing favorable compensation performance. This scheme will find applications in the areas of rotation detection, optical communications, and so on.
文摘A pair of primers created from information of PmNOBⅢ genome DNA Sal I fragment produced a 355bp band by using Penaeus chinensis non occluded baculovirus (PcNOBV),the WSBV isolate from P.hinensis in China's Mainland,as the DNA template.The specific PCR product was cloned,sequenced and labeled with digoxigenin (DIG)DNA labeling kit(Boehringer Mannheim).The DIG labeled fragment was tested by dot blot hybridization for sensitivity and specificity with purified PcNOBV nucleocapsid,PcNOBV infected shrimp tissues and healthy shrimp tissues.The detection limit of the DNA probe is 6.8pg of purified PcNOBV DNA.No hybridization signals were observed using DNA from healthy shrimp as template.Healthy P.chinensis,artificially infected P.chinensis and pond reared adult P.chinensis were screened for PcNOBV infection by both PCR and the hybridization assay.The results showed a good relationship between PCR and the hybridization assay.These findings demonstrate that the DIG labeled probe can be used as a sensitive,specific and cost effective reagent for detection of PcNOBV.
