目的分析陕西省急性弛缓性麻痹(acute flaccid paralysis,AFP)病例及接触者病毒分离和鉴定情况,为继续维持无脊髓灰质炎状态提供理论依据。方法采用描述性流行病学方法进行指标描述,将2014—2021年陕西省报告的AFP病例及接触者粪便标本...目的分析陕西省急性弛缓性麻痹(acute flaccid paralysis,AFP)病例及接触者病毒分离和鉴定情况,为继续维持无脊髓灰质炎状态提供理论依据。方法采用描述性流行病学方法进行指标描述,将2014—2021年陕西省报告的AFP病例及接触者粪便标本,采用转人类脊髓灰质炎病毒受体小鼠肺细胞(mouse cell line expressing the gene for human cellular receptor for Poliovirus,L20B)系和人横纹肌肉瘤(rhabdomyosarcoma,RD)细胞,根据WHO《脊髓灰质炎实验室手册》开展脊髓灰质炎病毒(Poliovirus,PV)检测,对L20B阳性分离物采取逆转录-聚合酶链式反应(RT-PCR)法扩增VP1区片段,同时开展核苷酸序列测定分析。结果从1104份AFP病例及接触者粪便标本检出7份PV阳性,14株PV,分离率为0.63%,分离非脊髓灰质炎肠道病毒(non-polio Enteroviruses,NPEV)36株,分离率为3.26%。相比昆明株和Sabin株,脊髓灰质炎病毒VP1区核苷酸<6个变异的为13株,1株发生9个变异;NPEV阳性分离例数7月和8月较多,占总数的30.56%。结论陕西省AFP监测系统运行良好,2014—2021年未发现野生型脊髓灰质炎病毒(wild Poliovirus,WPV)及疫苗衍生脊髓灰质炎病毒(vaccine-derived Poliovirus,VDPV),继续保持无脊髓灰质炎状态。展开更多
As a basic functional unit,living cell with sophisticated structures play an indispensable role in life activities.Since the abnormality of important molecules inside cells is closely related to diseases,the dynamic a...As a basic functional unit,living cell with sophisticated structures play an indispensable role in life activities.Since the abnormality of important molecules inside cells is closely related to diseases,the dynamic analysis and spatio-temporal monitoring of specific molecules in living cells can provide precious information for the diagnosis and treatment of diseases.More recently,DNA has not only been recognized as the carrier of genetic information,but has also used as a robust building block for the assembly of multitudinous nanoscale structures due to the intrinsic advantages of high programmability of classic Watson–Crick base-pairing rule.Intensive study promotes the rapid progress of nanotechnology in various fields,such as bioimaging,diagnosis,and therapeutics.Among numerous well-defined DNA nanomaterials,DNA nanomachines have been widely exploited in cell imaging owing to their desirable ability to achieve high-resolution temporal and spatial images in response to endogenous or exogenous stimuli.In brief,elaborate DNA nanomachines can undergo structural changes upon the stimuli of target analytes or environmental factors,resulting in rapid increase or reduction of output signals and thereby indirectly reflecting the expression level of targets.DNA nanomachines with high sensitivity and specificity contribute to the recognition of diseased tissues.In this review,we introduce the basic assembly modules of DNA nanomachines and summarize the recent advances in dynamic DNA nanomachines for diseased-cell imaging.Finally,the current challenges and future directions of DNA nanomachines for bioimaging are discussed.展开更多
文摘目的分析陕西省急性弛缓性麻痹(acute flaccid paralysis,AFP)病例及接触者病毒分离和鉴定情况,为继续维持无脊髓灰质炎状态提供理论依据。方法采用描述性流行病学方法进行指标描述,将2014—2021年陕西省报告的AFP病例及接触者粪便标本,采用转人类脊髓灰质炎病毒受体小鼠肺细胞(mouse cell line expressing the gene for human cellular receptor for Poliovirus,L20B)系和人横纹肌肉瘤(rhabdomyosarcoma,RD)细胞,根据WHO《脊髓灰质炎实验室手册》开展脊髓灰质炎病毒(Poliovirus,PV)检测,对L20B阳性分离物采取逆转录-聚合酶链式反应(RT-PCR)法扩增VP1区片段,同时开展核苷酸序列测定分析。结果从1104份AFP病例及接触者粪便标本检出7份PV阳性,14株PV,分离率为0.63%,分离非脊髓灰质炎肠道病毒(non-polio Enteroviruses,NPEV)36株,分离率为3.26%。相比昆明株和Sabin株,脊髓灰质炎病毒VP1区核苷酸<6个变异的为13株,1株发生9个变异;NPEV阳性分离例数7月和8月较多,占总数的30.56%。结论陕西省AFP监测系统运行良好,2014—2021年未发现野生型脊髓灰质炎病毒(wild Poliovirus,WPV)及疫苗衍生脊髓灰质炎病毒(vaccine-derived Poliovirus,VDPV),继续保持无脊髓灰质炎状态。
基金supported by National Natural Science Foundation of China(NSFC)(grant NO:22174020).
文摘As a basic functional unit,living cell with sophisticated structures play an indispensable role in life activities.Since the abnormality of important molecules inside cells is closely related to diseases,the dynamic analysis and spatio-temporal monitoring of specific molecules in living cells can provide precious information for the diagnosis and treatment of diseases.More recently,DNA has not only been recognized as the carrier of genetic information,but has also used as a robust building block for the assembly of multitudinous nanoscale structures due to the intrinsic advantages of high programmability of classic Watson–Crick base-pairing rule.Intensive study promotes the rapid progress of nanotechnology in various fields,such as bioimaging,diagnosis,and therapeutics.Among numerous well-defined DNA nanomaterials,DNA nanomachines have been widely exploited in cell imaging owing to their desirable ability to achieve high-resolution temporal and spatial images in response to endogenous or exogenous stimuli.In brief,elaborate DNA nanomachines can undergo structural changes upon the stimuli of target analytes or environmental factors,resulting in rapid increase or reduction of output signals and thereby indirectly reflecting the expression level of targets.DNA nanomachines with high sensitivity and specificity contribute to the recognition of diseased tissues.In this review,we introduce the basic assembly modules of DNA nanomachines and summarize the recent advances in dynamic DNA nanomachines for diseased-cell imaging.Finally,the current challenges and future directions of DNA nanomachines for bioimaging are discussed.
