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鸭瘟病毒Cycleave荧光PCR检测方法的建立
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作者 于新友 李天芝 苗立中 《中国动物传染病学报》 北大核心 2025年第2期106-110,共5页
为建立一种敏感、快速和特异的鸭瘟病毒(DPV)检测方法,本研究根据NCBI收录的DPV UL6基因序列,设计了1对特异性引物和1条Cycling探针,通过优化反应条件,建立了检测DPV的Cycleave荧光PCR检测方法。结果显示:该方法特异性高,不与其他常见... 为建立一种敏感、快速和特异的鸭瘟病毒(DPV)检测方法,本研究根据NCBI收录的DPV UL6基因序列,设计了1对特异性引物和1条Cycling探针,通过优化反应条件,建立了检测DPV的Cycleave荧光PCR检测方法。结果显示:该方法特异性高,不与其他常见鸭病病原体发生交叉反应,检测DPV灵敏度可达6.5拷贝/μL,批内与批间的变异系数均小于2%。研究表明,建立的DPV Cycleave荧光定量PCR方法特异性高、敏感性高、重复性好,可用于临床样品检测,为鸭瘟的诊断和防控奠定了基础。 展开更多
关键词 鸭瘟病毒 cycling探针 cycleave荧光PCR 检测
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Study on time effect and prediction model of shear strength of root-soil complex under dry-wet cycle 被引量:1
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作者 Zhengjun Mao Xu Ma +4 位作者 Yuncen Liu Mimi Geng Yanshan Tian Jiewen Sun Zhijie Yang 《Biogeotechnics》 2024年第2期54-67,共14页
Triaxial compression tests were conducted on the alfalfa root-loess complex at different growthperiods obtained through artificial planting.The research focused on analyzing the time variation law of the shear strengt... Triaxial compression tests were conducted on the alfalfa root-loess complex at different growthperiods obtained through artificial planting.The research focused on analyzing the time variation law of the shear strength index and deformation index of the alfalfa root-loess complex under dry-wet cycles.Additionally,the time effect of the shear strength index of the alfalfa root-loess complex under dry-wet cycles was analyzed and its prediction model was proposed.The results show that the PG-DWC(dry-wet cycle caused by plant water management during plant growth period)causes the peak strength of plain soil to change in a"V"shape with the increase of growth period,and the peak strength of alfalfa root-loess complex is higher than that of plain soil at the same growth period.The deterioration of the peak strength of alfalfa root-loess complex in the same growth period is aggravated with the increase of drying and wetting cycles.Compared with the 0 days growth period,the effective cohesion of alfalfa root-loess complex under different dry-wet cycles maximum increase rate is at the 180 days,which are 33.88%,46.05%,30.12%and 216.02%,respectively.When the number of dry-wet cycles is constant,the effective cohesion of the alfalfa root-loess complex overall increases with the growth period.However,it gradually decreases comparedwith the previous growth period,and the minimum increase rate are all at the 180 days.For the same growth period,the effective cohesion of the alfalfa root-loess complex decreases with the increase of the number of dry-wet cycles.This indicates that EC-DWC(the dry-wet cycles caused by extreme natural conditions such as continuous rain)have a detrimental effect on the time effect of the shear strength of the alfalfa root-loess complex.Finally,based on the formula of total deterioration,a prediction model for the shear strength of the alfalfa root-loess complex under dry-wet cycles was proposed,which exhibits high prediction accuracy.The research results provide useful guidance for the understanding of mechanical behavior and structural damage evolution of root-soil composite. 展开更多
关键词 Dry-wet cycle Root-soil complex Shear strength time effect Prediction model AlfalfaLoess
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Precise Control of the Recollision Dynamics in Nonsequential Double Ionization by Spatially Inhomogeneous Few-Cycle Negatively Chirped Laser Pulses
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作者 Yingbin Li Fengrun Wu +10 位作者 Fanfei Liu Shuaijie Kang Zhengfa Li Ke Zhang Yifan Liu Kuo Li Chunyang Zhai Jingkun Xu Pu Wang Qiming Zhao Benhai Yu 《Chinese Physics Letters》 2025年第5期26-32,共7页
With a three-dimensional semiclassical ensemble method, we theoretically investigated the nonsequential double ionization of Ar driven by the spatially inhomogeneous few-cycle negatively chirped laser pulses. Our resu... With a three-dimensional semiclassical ensemble method, we theoretically investigated the nonsequential double ionization of Ar driven by the spatially inhomogeneous few-cycle negatively chirped laser pulses. Our results show that the recollision time window can be precisely controlled within an isolated time interval of several hundred attoseconds, which is useful for understanding the subcycle correlated electron dynamics. More interestingly, the correlated electron momentum distribution (CEMD) exhibits a strong dependence on laser intensity. That is, at lower laser intensity, CEMD is located in the first quadrant. As the laser intensity increases,CEMD shifts almost completely to the second and fourth quadrants, and then gradually to the third quadrant.The underlying physics governing the CEMD's dependence on laser intensity is explained. 展开更多
关键词 recollision time window recollision dynamics correlated electron momentum distribution nonsequential double ionization precise control understanding subcycle correlated electron dynamics three dimensional semiclassical ensemble method spatially inhomogeneous few cycle negatively chirped laser pulses
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Effects of Serum Concentration, Synchronization Time and Confluence on the Cell-Cycle Synchronization Efficiency of Goat Fibroblasts
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作者 Van Khanh Nguyen Huong Thu Thi Vu +4 位作者 Au Thi Hoang Yen Kim Thi Pham Giang Thi Thanh Nhan Hung Phu Lai Lan Doan Pham 《Open Journal of Animal Sciences》 2024年第3期194-203,共10页
This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference... This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference in the percentage of nucleated fibroblasts in the G0/G1 stage between serum concentrations of 0.3% and 0.4% (83.89% and 82.69%, respectively, P > 0.05) as well as between serum concentrations of 0.2% and 0.5% (76.95% and 75.46%, respectively, P > 0.05). The percentage of nucleated fibroblasts in the G0/G1 stage was highest at the concentration of 0.3% and lowest in the control group (83.89% vs. 62.67%, P 0.05). The beneficial effect of high confluence was confirmed by the large percentage of nucleated fibroblasts at the G0/G1 stage. The 60% confluency was significantly lower than the 80% and 100% confluency (73.44%, 86.63%, and 87.17%, respectively, P < 0.05). The results indicate that the goat fibroblast cycle synchronization is the most effective at the serum concentration of 0.3%, 72 hours of synchronization and 100% confluency. 展开更多
关键词 Goat Fibroblast cycle Synchronisation Serum Concentration Synchronization time CONFLUENCE
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CycleLLH:一种基于周期性整合的新型网络流量预测模型
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作者 唐文杰 肖一磊 +3 位作者 孔祥宇 齐恒 刘秀龙 李克秋 《计算机学报》 EI CAS CSCD 北大核心 2024年第12期2867-2888,共22页
精准的网络流量预测是实现网络精细化和智能化管理的关键,也是网络运营商、云服务提供商等实现网络智能运维及应用服务保障的重要支撑,属于当前业界研究的热点.网络流量预测问题一般可被视为一种时间序列预测问题,现有时间序列预测模型... 精准的网络流量预测是实现网络精细化和智能化管理的关键,也是网络运营商、云服务提供商等实现网络智能运维及应用服务保障的重要支撑,属于当前业界研究的热点.网络流量预测问题一般可被视为一种时间序列预测问题,现有时间序列预测模型虽然能起到一定作用,但这些通用模型很少考虑流量数据集本身特点,从而无法在网络流量预测性能上取得突破.为此,本文重点研究了网络流量数据集中的自然周期特征,提出了一种能有效利时间序列周期性特点的网络流量预测通用模型——Cycle Little Linear Head(CycleLLH).该模型主干为Transformer的编码器,其中两个关键设计在于:(1)周期整合.将流量序列按照一个特定周期划分步长划分为不同的周期块,然后将这些周期块对应相位的时间节点分别嵌入到不同输入令牌;(2)小线性层.由多个多层感知机组成,并且多层感知机单独作用于每个相位的时间特征.周期整合使得模型具有两个优点:更有利于模型提取数据集在一个周期内的特征;注意力矩阵的计算和内存复杂度可以看作是和周期划分步长二次方有关的常数,使得模型可以使用更大的回溯窗口而仅增加少量计算资源.通过在公共流量数据集上进行大量实验,本研究表明:与当前最先进的模型相比,CycleLLH在流量预测精度方面表现出显著优势,在六个数据集上的预测精度分别提升了12.3%、8.4%、29.9%、5.8%、8.3%和2.0%.代码可从https://github.com/wenjietang218/CycleLLH.git中获取. 展开更多
关键词 网络流量预测 时间序列预测 周期性 周期整合 小线性层
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A discrete-event model to simulate the effect of truck bunching due to payload variance on cycle time, hauled mine materials and fuel consumption 被引量:2
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作者 A.Soofastaei S.M.Aminossadati +1 位作者 M.S.Kizil P.Knights 《International Journal of Mining Science and Technology》 SCIE EI CSCD 2016年第5期745-752,共8页
Data collected from truck payload management systems at various surface mines shows that the payload variance is significant and must be considered in analysing the mine productivity,energy consumption,greenhouse gas ... Data collected from truck payload management systems at various surface mines shows that the payload variance is significant and must be considered in analysing the mine productivity,energy consumption,greenhouse gas emissions and associated cost.Payload variance causes significant differences in gross vehicle weights.Heavily loaded trucks travel slower up ramps than lightly loaded trucks.Faster trucks are slowed by the presence of slower trucks,resulting in‘bunching’,production losses and increasing fuel consumptions.This paper simulates the truck bunching phenomena in large surface mines to improve truck and shovel systems’efficiency and minimise fuel consumption.The study concentrated on completing a practical simulation model based on a discrete event method which is most commonly used in this field of research in other industries.The simulation model has been validated by a dataset collected from a large surface mine in Arizona state,USA.The results have shown that there is a good agreement between the actual and estimated values of investigated parameters. 展开更多
关键词 Discrete-event model Simulation Truck bunching Payload variance cycle time Fuel consumption
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Investigation of Excavator Performance Factors in an Open-Pit Mine Using Loading Cycle Time 被引量:1
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作者 Samwel Victor Manyele 《Engineering(科研)》 2017年第7期599-624,共26页
This study presents the effect of excavator model, loading operation location, shift availability and truck-shovel combination on loading cycle time and productivity of an open-pit mine. The loading cycle time was use... This study presents the effect of excavator model, loading operation location, shift availability and truck-shovel combination on loading cycle time and productivity of an open-pit mine. The loading cycle time was used to assess the material loading system performance which is one of the key components of the total cycle time for material transportation in an open-pit mine. Loading is among the components of cycle time during which material is being handled. The data analyzed?was?collected from a computerized dispatch system at GGM from which 62,000 loading dispatches per month involving several shifts, 14 excavators and 49 trucks were loaded. About 4465 dispatches per excavator and 1276 dispatches per truck were assessed using loading cycle time data for each dispatch for a period of four months (between August and December). Under fixed tonnage loaded and waste type (33 t of non-acid forming waste rock),?it was observed that loading cycle time depends on excavator model, location and truck being loaded. Average cycle times, PDFS?and CDFS of loading cycle time series were used to identify differences in performance under different situations. It was concluded that shift availability for excavators, loading location, excavator model and truck-shovel combinations strongly affect the productivity during loading process in an open-pit mine. 展开更多
关键词 Potentially Acid-Forming (PAF) ROCK Non-Acid FORMING (NAF) ROCK TRUCK Utilization TRUCK Fill-Factor Queuing time LOADING cycle time Full and Empty Haul Total cycle time Theoretical cycle time non-productive cycle time cycle time Performance Ratio
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Analysis of Waste-Rock Transportation Process Performance in an Open-Pit Mine Based on Statistical Analysis of Cycle Times Data 被引量:1
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作者 Samwel Victor Manyele 《Engineering(科研)》 2017年第7期649-679,共31页
In this paper, the performance of a waste rock transportation process in an open pit mine was assessed by using cycle time data. A computerized truck-excavator dispatch system was used to record the cycle times. The p... In this paper, the performance of a waste rock transportation process in an open pit mine was assessed by using cycle time data. A computerized truck-excavator dispatch system was used to record the cycle times. The process was broken into seven steps (or components of the total cycle), durations of which were recorded for a period of 1 month, leading to N = 60,690 data points or dispatches. The open pit mine studied consisted of 12 waste types loaded by 14 excavators and hauled by 49 trucks (at a trucks-to-excavator ratio of 3.5:1) in 75 changing locations. The string-type data was coded using integers to allow a FORTRAN code to extract process performance parameters using statistical analysis. The study established a wide range of parameters including: the waste material generation rate (about 1.73 million t/month, 81% comprising waste rock), truck fill factor, f, total cycle time (Tct), production capacity, theoretical cycle time, non-productive cycle time Tnp, and cycle time performance ratio (CTPR), denoted as Tpr. The factors affecting the process performance include: truck model, excavator model, location (haul distance and road conditions) and material type. For a fixed material type and tonnage, the PDFs of the cycle time components were logarithmic in nature, capable of differentiating performance variations under different factors. It was concluded that the performance of the waste material transportation system in this mine was determined to be acceptable due to mean value of Tpr = 2.432 being closer to unity. Reduction measures were suggested to minimize the cycle time for the process bottlenecks determined from Pareto analysis (that is, full haul, empty haul and loading processes). 展开更多
关键词 Potentially Acid-Forming (PAF) ROCK Non-Acid FORMING (NAF) ROCK TRUCK Utilization TRUCK Fill-Factor Queuing time Loading cycle time Full and EMPTY Haul Total cycle time Theoretical cycle time non-productive cycle time cycle time Performance Ratio
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Analysis of Medical Waste Incinerator Performance Based on Fuel Consumption and Cycle Times 被引量:2
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作者 Samwel Victor Manyele Ignatio Simon Kagonji 《Engineering(科研)》 2012年第10期625-635,共11页
A detailed assessment of an incinerator based on fuel consumption and cycle time data is presented in this paper. The study was conducted at Temeke district hospital for 22 months consecutively covering 654 days of da... A detailed assessment of an incinerator based on fuel consumption and cycle time data is presented in this paper. The study was conducted at Temeke district hospital for 22 months consecutively covering 654 days of daily data collection on fuel consumption and cycle times. The composition for the medical waste incinerated varied between 15% and 35% for sharps waste and between 65% and 85% for other waste, with mean values of 25% and 75%, respectively. The results revealed poor performance of the incinerator due to higher fuel consumption (above 30 L/cycle). The incineration cycle times were observed to range between 2 and 4 hours, all of which were too high for the loading rates observed (55 - 214 kg). A strong dependency of diesel oil consumption on cycle time was observed due to lack of temperature control leading to continuous fuel flow into the burners. The incineration capacity was very low compared to other incinerators in terms of tons per year. This paper gives an insight on the factors affecting incinerator performance assessed based on diesel oil consumption and cycle times. It can be generalized that the incinerator performance was poor due to several factors ranging from poor incinerator design, operator skills, waste management practices, waste storage practices, etc. The hospital was advised to install a new incinerator with short incineration cycle time (30 - 40 minutes) and lower fuel consumption (10 L/cycle) at a loading rate of 200 kg/cycle. 展开更多
关键词 WASTE Combustion INCINERATOR PERFORMANCE Medical WASTE INCINERATION FUEL CONSUMPTION INCINERATION cycle time Sharps WASTE INCINERATOR Capacity FUEL Effectiveness
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INFLUENCE OF DWELL TIME ON HIGH TEM-PERATURE LOW CYCLE FATIGUE (HTLCF) BEHAVIOR IN AN Nd-BEARING NEAR-α TITANIUM ALLOY
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作者 Zhu Zhishou, Cao Chunxiao, Ma Jimin, Gao Yang, Yan Minggao (Beijing Institute of Aeronautical Materials, Beijing, 100095, China) 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 1997年第3期42-47,共6页
The influences of strain amplitude ranges and dwell time at peak strains on the low cycle fatigue (LCF) properties at 600℃ of a new near α high temperature titanium alloy containing rare earth Nd are investigated. ... The influences of strain amplitude ranges and dwell time at peak strains on the low cycle fatigue (LCF) properties at 600℃ of a new near α high temperature titanium alloy containing rare earth Nd are investigated. The creep fatigue interaction behavior is discussed in this paper in terms of a creep fatigue interaction cumulative law and fatigue crack propagation model. The results show that the creep fatigue interaction is largely dependent on the strain amplitude range, and the tensile dwell periods, as well as compressive dwell periods, have a great influence on the LCF life of this alloy. 展开更多
关键词 creep analysis fatigue (materials) titanium alloys dwell time low cycle fatigue (LCF)
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Independent Cycle Time Assignment for Min-max Systems
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作者 Wen-De Chen Yue-Gang Tao Hong-Nian Yu 《International Journal of Automation and computing》 EI 2010年第2期254-260,共7页
A variety of problems in digital circuits, computer networks, automated manufacturing plants, etc., can be modeled as min-max systems. The cycle time is an important performance metric of such systems. In this paper, ... A variety of problems in digital circuits, computer networks, automated manufacturing plants, etc., can be modeled as min-max systems. The cycle time is an important performance metric of such systems. In this paper, we focus on the cycle time assignment of minimax systems which corresponds to the pole assignment problem in traditional linear control systems. For the min- max system with max-plus inputs and outputs, we show that the cycle time can be assigned disjointedly by a state feedback, if and only if the system is reachable. Furthermore, a necessary and sufficient condition for the cycle time to be assigned independently by a state feedback is given. The methods are constructive, and some numerical examples are given to illustrate how the methods work in practice. 展开更多
关键词 cycle time coloring graph independent assignment min-max systems state feedback.
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Real-time Life-cycle Modular Design Method Based on Game Theory for Jack-up
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作者 郑玄亮 王运龙 林焰 《Journal of Shanghai Jiaotong university(Science)》 EI 2010年第4期504-511,共8页
To study an entity the entire jack-up performs as a total system with close interdependency and high level of integration of a myriad of subsystems supporting each individual function. All subsystems should be arrange... To study an entity the entire jack-up performs as a total system with close interdependency and high level of integration of a myriad of subsystems supporting each individual function. All subsystems should be arranged in regular geometric space with clear regional division according to their respective functions. The "building block" such as arrangement makes it feasible to apply modular design method while the integration and conflicts among subsystems offer an arena for game theoretic analysis. All the contradictions between subsystems should be resolved bye the designer. Here we present a game theoretic analysis methodology, which based on the basic principles of game theory, through the establishment of matrix-mode game models to describe and analyze engineering design tasks. By using this method the optimization process in various fields of expertise can be effectively coordinated and controlled, and the parallel design patterns can be achieved to some extent. Taking account of the impact of commercial or technical activities in platform's life cycle, time line is considered in the early stage of design process which provides more convenience for efficient upgrade and reconfiguration. Some second-hand three-legged jack-up upgrade design process is used as an example to illustrate the utility of the method. 展开更多
关键词 jack up life cycle design modular design real time game model
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Closed and Open Metabolic Cycles: Transition Time
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作者 Antonio Sillero Víctor García-Herrero 《Journal of Biomedical Science and Engineering》 2016年第2期127-140,共14页
A metabolic cycle can be viewed as a central core and its branches. The central core is here firstly considered as a pre-closed metabolic cycle (CMC), with a unique first substrate, but with no input or output of othe... A metabolic cycle can be viewed as a central core and its branches. The central core is here firstly considered as a pre-closed metabolic cycle (CMC), with a unique first substrate, but with no input or output of other components. By contrast, the metabolic cycles in nature are open metabolic cycles (OMC) with output and input of external substrates (through “metabolic branches”), modulating continuously the enzyme activities and the total concentration of their substrates thorough complex regulatory phenomena. In this work, the transition from a Closed to an Open metabolic cycle has been simulated by a consecutive entry and exit of two components through the catalytic action of two enzymes. It is known that after any alteration of the initial conditions, the cycles need a time to reach new equilibrium. We have measured the changes of transition time (T.T.) values in 81 models of CMC differing in Km or Vmax values. In general, the T.T. tends to be shorter in cycles with preponderant lower Km and higher Vmax values. Further, Mathematica refinement for the estimation of transition time from the data previously calculated can be obtained with the use of the command Interpolating Function. 展开更多
关键词 Metabolic cycles Equilibration times Kinetic Constants Differential Equations MATHEMATICA
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Analysis of Temperature Profiles and Cycle Time in a Large-Scale Medical Waste Incinerator
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作者 Veilla E. Matee Samwel V. Manyele 《Engineering(科研)》 2015年第10期717-732,共16页
Temperature profiles and cycle times in a large-scale medical waste incinerator installed in a referral hospital were used to assess the performance and functionality of incinerator. The study was conducted using data... Temperature profiles and cycle times in a large-scale medical waste incinerator installed in a referral hospital were used to assess the performance and functionality of incinerator. The study was conducted using data collected from 8 cycles per days for 67 days. For proper combustion and destruction of toxic components in the primary chamber and destruction of pollutants and toxic components in the flue gas, it is desired to reach the maximum temperature in the chambers faster and maintain this maximum temperature for an extended time interval. The primary and secondary temperatures T1 and T2, respectively, were recorded at an interval of one minute for different cycles. Different amounts of wastes with varying proportions of sharps and other wastes were loaded into the incinerator and temperature profiles recorded. The analysis shows that the incinerator works at primary temperature less than the required recommended by manufacturer while the secondary chamber operates between 600 and above 950℃, although higher temperatures up to 1020℃ were observed. The average load preparation time was observed to be 14.6 minutes, while the chamber preheating time before daily initial loading was 25.45 minutes. Both temperature profiles were observed to have similar shapes for all combustion cycles studied, except when incinerator malfunctioning occurred. The average cycle time was established to be 32.7 minutes and 28.97 minutes based on time to drop to 550℃ after the maximum temperature and loading time intervals, respectively, although longer cycle times were observed. Temperature drop in both combustion chambers as a result of waste charging was observed in the interval of 5 minutes. The chamber heating rate was observed to decrease exponentially with time during both preheating and incineration operation. 展开更多
关键词 Medical WASTE Management INFECTIOUS WASTE PATHOLOGICAL WASTE Sharps WASTE INCINERATION Temperature Profile cycle time HEATING Rate
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Key Dolerite Dyke Swarms of Amazonia:U-Pb Constraints on Supercontinent Cycles and Geodynamic Connections with Global LIP Events Through Time
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作者 Wilson TEIXEIRA Mike A.HAMILTON +1 位作者 Vicente A.V.GIRARDI Frederico M.FALEIROS 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2016年第S1期84-85,共2页
High-resolution U–Pb(ID-TIMS,baddeleyite)ages are presented for mafic dykes from selected swarms in two important Amazonian regions:the Carajás Province in the east,and the Rio Apa block in the southwest–areas
关键词 Pb U-Pb Constraints on Supercontinent cycles and Geodynamic Connections with Global LIP Events Through time Key Dolerite Dyke Swarms of Amazonia LIP
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Cycle Time Analysis of Open Pit Mining Dump Trucks
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作者 Baatarchuluun Enkhchuluun Bat-Ochir Batgerel Cao Ping 《International Journal of Geosciences》 2023年第8期689-709,共21页
This study demonstrates a practical cycle time analysis of dump truck haulage system of “Ukhaa Khudag” open-pit coal mine located in Umnugobi Province, Mongolia. It examines the possibility of minimizing the cycle t... This study demonstrates a practical cycle time analysis of dump truck haulage system of “Ukhaa Khudag” open-pit coal mine located in Umnugobi Province, Mongolia. It examines the possibility of minimizing the cycle time of the haulage system as well as factors impacting the speed of the dump truck. The current study divides the open pit mine road for the dump trucks into five sections which are bench road, ramp, surface road, dump road uphill, and dump road. Meanwhile, it investigates the influence of the length, the grade, and the rolling resistance of the road section on the cycle time. The data is analyzed using mathematical regression methods via Microsoft Excel program. For each of the five road sections, we compare the statistical calculations of three regression models: linear, quadratic and exponential;thus, a total of thirty regression models are obtained in this research. Accordingly, the cycle time for each road section is predicted by the most accountable model. The loaded and empty direction of the movement is measured and calculated for each road section, and it appears that the difference between the calculated mean value and the actual cycle time of the models is 0.82 seconds with a relative error of 2.51 percent. 展开更多
关键词 Dump Truck cycle time Mine Haul Road Regression Analysis
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梨衰退植原体Cycleave实时荧光PCR检测方法的建立 被引量:1
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作者 李鑫 王有福 +3 位作者 刘卉秋 曹冬梅 胡强 王秀芬 《植物病理学报》 CAS CSCD 北大核心 2013年第4期350-353,共4页
根据植原体16S rDNA保守区设计Cycling探针LST2probe及引物,建立了梨衰退植原体Cycleave实时荧光PCR检测方法。结果表明,探针LST2probe能特异的检测梨衰退植原体,供试同一组内不同亚组植原体及参试病原细菌均为阴性,检测灵敏度可达0.5 ... 根据植原体16S rDNA保守区设计Cycling探针LST2probe及引物,建立了梨衰退植原体Cycleave实时荧光PCR检测方法。结果表明,探针LST2probe能特异的检测梨衰退植原体,供试同一组内不同亚组植原体及参试病原细菌均为阴性,检测灵敏度可达0.5 pg/μL。该Cycleave实时荧光PCR检测方法可用于梨衰退植原体的快速检测,并为其他有害生物鉴定提供借鉴依据。 展开更多
关键词 梨衰退植原体 实时荧光PCR cycling探针
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Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells 被引量:7
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作者 Zhi-Zhong Liu Wen-Ying Huang +3 位作者 Ju-Sheng Lin Xiao-Sheng Li Kuo-Huan Liang Jia-Long Huang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第44期7033-7035,共3页
AIM: To evaluate the change of growth characteristics and radiosensitivity of irradiated primary cultured human hepatocarcinoma cells. METHODS: All tumor tissue samples were obtained from 39 hepatocarcinoma patients... AIM: To evaluate the change of growth characteristics and radiosensitivity of irradiated primary cultured human hepatocarcinoma cells. METHODS: All tumor tissue samples were obtained from 39 hepatocarcinoma patients with a mean age of 49.6 years (range 22-76 years). We divided the samples into irradiated group and non-irradiated group and measured their plating efficiency (PE), population doubling time (PDT), radiosensitivity index SF2 and cell RESULTS: The PDT of primary culture of hepatocardnoma cells was 91.0±6.6 h, PE was 12.0±1.4%, SF2 was 0.41±0.05%. The PDT of their inadiated progeny was 124.8±5.8 h, PE was 5.0±0.7%, SF2 was 0.65±0.09%. The pdmary cultured human hepatocarcinoma cells showed significant S reduction and G^2 arrest in a dose-dependent manner. The progeny of irradiated primary cultured hepatocarcinoma cells grew more slowly and its radiosensitivity increased. CONCLUSION: The progeny of irradiated primary cultured human hepatocarcinoma cells grows more slowly and its radiosensitivity increases. 展开更多
关键词 HEPATOCARCINOMA Cell cycle Population doubling time RADIOSENSITIVITY
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Th–U cycle performance analysis based on molten chloride salt and molten fluoride salt fast reactors 被引量:4
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作者 Liao-Yuan He Shao-Peng Xia +4 位作者 Xue-Mei Zhou Jin-Gen Chen Gui-Min Liu Yang Zou Rui Yan 《Nuclear Science and Techniques》 SCIE CAS CSCD 2020年第8期116-128,共13页
The recent development of molten salt fast reactors has generated a renewed interest in them. As compared to traditional solid fuel fast neutron systems, it has many unique advantages, e.g., lower fissile inventory,no... The recent development of molten salt fast reactors has generated a renewed interest in them. As compared to traditional solid fuel fast neutron systems, it has many unique advantages, e.g., lower fissile inventory,no initial criticality reserve, waste reduction, and a simplified fuel cycle. It has been recognized as an ideal reactor for achieving a closed Th–U cycle. Based on the carrier salt, molten salt fast reactors could be divided into either a molten chloride salt fast reactor(MCFR) or a molten fluoride salt fast reactor(MFFR);to compare their Th–U cycle performance, the neutronic parameters in a breeding and burning(B&B) transition scenario were studied based on similar core geometry and power. The results demonstrated that the required reprocessing rate for an MCFR to achieve self-breeding was lower than that of an MFFR.Moreover, the breeding capability of an MCFR was better than that of an MFFR;at a reprocessing rate of 40 L/day,using LEU and Pu as start-up fissile materials, the doubling time(DT) of an MFFR and MCFR were 88.0 years and 48.0 years, and 16.5 years and 16.2 years, respectively.Besides, an MCFR has lower radio-toxicity due to lower buildup of fission products(FPs) and transuranium(TRU),while an MFFR has a larger, delayed neutron fraction with smaller changes during the entire operation. 展开更多
关键词 Th–U cycle Molten salt fast reactor Breeding capability Doubling time
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Relation between Solar Wind Parameter and Geomagnetic Storm Condition during Cycle-23 被引量:2
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作者 Balveer S. Rathore Dinesh C. Gupta K. K. Parashar 《International Journal of Geosciences》 2014年第13期1602-1608,共7页
In the present paper dependence of geomagnetic activity on the solar-wind plasma and interplanetary magnetic field (IMF) parameters has been studied. We have taken interplanetary solar wind data at the instant of Dst ... In the present paper dependence of geomagnetic activity on the solar-wind plasma and interplanetary magnetic field (IMF) parameters has been studied. We have taken interplanetary solar wind data at the instant of Dst minimum. Our study consists of 200 geomagnetic storms weighed by disturbance storm time (Dst) -50 nT, observed during solar cycle 23. The study suggests that the strength of the geomagnetic storm is strongly dependent on the total magnetic field Btotal. The correlation (-0.72) has been found reasonable. In perspective of previous studies, the strength of the geomagnetic storm is strongly dependent on the southward component (Bz) whereas in present study exposes that the correlation (0.22) is weak. This result indicates that solar wind southward magnetic field component Bz has significant growth particularly before the main phase of geomagnetic storm (not during the main phase). The present result implies that neither density nor temperature is significantly related to the variation of geomagnetic disturbance;rather the effects of the pressure and speed. However, a low plasma beta during highly geoeffective event seems to be an important criterion. 展开更多
关键词 GEOMAGNETIC STORM INTERPLANETARY Magnetic Field (IMF) DISTURBANCE STORM time (Dst) Solar cycle
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