DNA methylation at non-CG dinucleotides(mCH,H=A,C,T)widely occurs and plays an important role in specific cell types,including pluripotent,neural,and germ cells.However,the functions and regulatory mechanisms of mCH,p...DNA methylation at non-CG dinucleotides(mCH,H=A,C,T)widely occurs and plays an important role in specific cell types,including pluripotent,neural,and germ cells.However,the functions and regulatory mechanisms of mCH,particularly in species other than humans and mice,remain inadequately explored.In this study,we analyzed the distribution of mCH across different bovine tissues,identifying significantly elevated mCH levels in bovine embryonic stem cells(bESCs),as well as brain,spleen,and ileum tissues compared to other tissues.Marked differences in mCH patterns between somatic cells and bESCs were observed,reflecting distinct base preferences and the differential expression of DNA methyltransferases.We also identified exon methylation in both CG and nonCG contexts,resembling gene-associated methylation patterns observed in plants.To characterize tissue-specific variations in mCH,we developed a novel method for differential mCH analysis.Results indicated that mCH is not randomly distributed but tends to be enriched in tissuespecific functional regions.Furthermore,regression models demonstrated a positional correlation between CG methylation and mCH.This study enhances our understanding of mCH distribution and function in bovine somatic and stem cells,providing new insights into its potential roles across species and tissues.These findings advance knowledge of epigenetic mechanisms,shedding light on the potential involvement of mCH in development and disease processes.展开更多
A hybrid method combining simplified sub-entire domain basis function method of moment with finite element method( SSED-MoM /FEM) is accelerated for electromagnetic( EM) scattering analysis of large-scale periodic str...A hybrid method combining simplified sub-entire domain basis function method of moment with finite element method( SSED-MoM /FEM) is accelerated for electromagnetic( EM) scattering analysis of large-scale periodic structures.The unknowns are reduced sharply with non-uniform mesh in FEM. The computational complexity of the hybrid method is dramatically declined by applying conjugate gradient-fast Fourier transform( CG-FFT) to the integral equations of both electric field and magnetic field. The efficiency is further improved by using OpenMP technique. Numerical results demonstrate that the SSED-MoM /FEM method can be accelerated for more than three thousand times with large-scale periodic structures.展开更多
基金supported by the STI 2030-Major Projects(2023ZD0407504)of ChinaDevelopment Plan for Young Scientific and Technological Talents in Colleges and Universities of Inner Mongolia Autonomous Region of China(NMGIRT2204)+1 种基金National Natural Science Foundation of China(32160172)Science and Technology Major Project of the Inner Mongolia Autonomous Region of China to the State Key Laboratory of Reproductive Regulation(2021ZD0048&2023KYPT0010)。
文摘DNA methylation at non-CG dinucleotides(mCH,H=A,C,T)widely occurs and plays an important role in specific cell types,including pluripotent,neural,and germ cells.However,the functions and regulatory mechanisms of mCH,particularly in species other than humans and mice,remain inadequately explored.In this study,we analyzed the distribution of mCH across different bovine tissues,identifying significantly elevated mCH levels in bovine embryonic stem cells(bESCs),as well as brain,spleen,and ileum tissues compared to other tissues.Marked differences in mCH patterns between somatic cells and bESCs were observed,reflecting distinct base preferences and the differential expression of DNA methyltransferases.We also identified exon methylation in both CG and nonCG contexts,resembling gene-associated methylation patterns observed in plants.To characterize tissue-specific variations in mCH,we developed a novel method for differential mCH analysis.Results indicated that mCH is not randomly distributed but tends to be enriched in tissuespecific functional regions.Furthermore,regression models demonstrated a positional correlation between CG methylation and mCH.This study enhances our understanding of mCH distribution and function in bovine somatic and stem cells,providing new insights into its potential roles across species and tissues.These findings advance knowledge of epigenetic mechanisms,shedding light on the potential involvement of mCH in development and disease processes.
基金Supported by the Aeronautical Science Foundation of China(20121852031)
文摘A hybrid method combining simplified sub-entire domain basis function method of moment with finite element method( SSED-MoM /FEM) is accelerated for electromagnetic( EM) scattering analysis of large-scale periodic structures.The unknowns are reduced sharply with non-uniform mesh in FEM. The computational complexity of the hybrid method is dramatically declined by applying conjugate gradient-fast Fourier transform( CG-FFT) to the integral equations of both electric field and magnetic field. The efficiency is further improved by using OpenMP technique. Numerical results demonstrate that the SSED-MoM /FEM method can be accelerated for more than three thousand times with large-scale periodic structures.
