Structured illumination microscopy has been a useful method for achieving lateral super-resolution,but it typically requires at least three precise phase shifts per orientation.In this paper,we propose a super-resolut...Structured illumination microscopy has been a useful method for achieving lateral super-resolution,but it typically requires at least three precise phase shifts per orientation.In this paper,we propose a super-resolution method that utilizes structured illumination without phase shift.The reconstruction process requires only a conventionally illuminated image and an image with structured illumination.This method achieves the same effect as the traditional phase shift method,and more than doubles the resolution by synthesizing a few reconstructions at different illumination frequencies.We verify the resolution improvement process using a combination of theoretical derivations and diagrams,and demonstrate its effectiveness with numerical simulations.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.51775381)National Key Scientific Instrument and Equipment Development Project(Grant No.2017YFF0107001)
文摘Structured illumination microscopy has been a useful method for achieving lateral super-resolution,but it typically requires at least three precise phase shifts per orientation.In this paper,we propose a super-resolution method that utilizes structured illumination without phase shift.The reconstruction process requires only a conventionally illuminated image and an image with structured illumination.This method achieves the same effect as the traditional phase shift method,and more than doubles the resolution by synthesizing a few reconstructions at different illumination frequencies.We verify the resolution improvement process using a combination of theoretical derivations and diagrams,and demonstrate its effectiveness with numerical simulations.
