This work presents a promising clinical molecular diagnostics for early stage lung cancer.This novel diagnostic method utilized the loopmediated isothermal amplification(LAMP),micro fluidic chips and a confocal optica...This work presents a promising clinical molecular diagnostics for early stage lung cancer.This novel diagnostic method utilized the loopmediated isothermal amplification(LAMP),micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor.An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability.Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min.While the traditional intube RTPCR set consumed more than 25-μL of the assay and took more than 90 rain.展开更多
基金supported by the National Supporting Plan of China(2011BAZ03288,2012BAI23B00)the National Program on Key Basic Research Projects of China(2011CB707701)+2 种基金the National Natural Science Foundation of China(60978064)the Science Plan Foundation of Zhejiang(2012C31G3210006)the TSINGHUA-SIEMENS Collective Foundation(20103000273)
文摘This work presents a promising clinical molecular diagnostics for early stage lung cancer.This novel diagnostic method utilized the loopmediated isothermal amplification(LAMP),micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor.An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability.Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min.While the traditional intube RTPCR set consumed more than 25-μL of the assay and took more than 90 rain.
