Male infertility can result from impaired sperm motility caused by multiple morphological abnormalities of the flagella(MMAF).Distinct projections encircling the central microtubules of the spermatozoal axoneme play p...Male infertility can result from impaired sperm motility caused by multiple morphological abnormalities of the flagella(MMAF).Distinct projections encircling the central microtubules of the spermatozoal axoneme play pivotal roles in flagellar bending and spermatozoal movement.Mammalian sperm-associated antigen 17(SPAG17)encodes a conserved axonemal protein of cilia and flagella,forming part of the C1a projection of the central apparatus,with functions related to ciliary/flagellar motility,skeletal growth,and male fertility.This study investigated two novel homozygous SPAG17 mutations(M1:NM_206996.2,c.829+1G>T,p.Asp212_Glu276del;and M2:c.2120del,p.Leu707*)identified in four infertile patients from two consanguineous Pakistani families.These patients displayed the MMAF phenotype confirmed by Papanicolaou staining and scanning electron microscopy assays of spermatozoa.Quantitative real-time polymerase chain reaction(PCR)of patients’spermatozoa also revealed a significant decrease in SPAG17 mRNA expression,and immunofluorescence staining showed the absence of SPAG17 protein signals along the flagella.However,no apparent ciliary-related symptoms or skeletal malformations were observed in the chest X-rays of any of the patients.Transmission electron microscopy of axoneme cross-sections from the patients showed incomplete C1a projection and a higher frequency of missing microtubule doublets 1 and 9 compared with those from fertile controls.Immunofluorescence staining and Western blot analyses of spermatogenesis-associated protein 17(SPATA17),a component of the C1a projection,and sperm-associated antigen 6(SPAG6),a marker of the spring layer,revealed disrupted expression of both proteins in the patients’spermatozoa.Altogether,these findings demonstrated that SPAG17 maintains the integrity of spermatozoal flagellar axoneme,expanding the phenotypic spectrum of SPAG17 mutations in humans.展开更多
With the help of the variable-coefficient generalized projected Ricatti equation expansion method, we present exact solutions for the generalized （2＋1）-dimensional nonlinear SchrSdinger equation with variable coeff...With the help of the variable-coefficient generalized projected Ricatti equation expansion method, we present exact solutions for the generalized （2＋1）-dimensional nonlinear SchrSdinger equation with variable coefficients. These solutions include solitary wave solutions, soliton-like solutions and trigonometric function solutions. Among these solutions, some are found for the first time.展开更多
With a new projective equation, a series of solutions of the （2-J-1）-dimensional dispersive long-water wave system （LWW） is derived. Based on the derived solitary wave solution, we obtain some special fractal loca...With a new projective equation, a series of solutions of the （2-J-1）-dimensional dispersive long-water wave system （LWW） is derived. Based on the derived solitary wave solution, we obtain some special fractal localized structures and chaotic patterns.展开更多
Social dysfunction is a risk factor for several neuropsychiatric illnesses.Previous studies have shown that the lateral septum(LS)-related pathway plays a critical role in mediating social behaviors.Howeve r,the role ...Social dysfunction is a risk factor for several neuropsychiatric illnesses.Previous studies have shown that the lateral septum(LS)-related pathway plays a critical role in mediating social behaviors.Howeve r,the role of the connections between the LS and its downstream brain regions in social behavio rs remains unclea r.In this study,we conducted a three-chamber test using electrophysiological and chemogenetic approaches in mice to determine how LS projections to ventral CA1(vCA1)influence sociability.Our res ults showed that gamma-aminobutyric acid(GABA)-e rgic neuro ns were activated following social experience,and that social behavio rs were enhanced by chemogenetic modulation of these neurons.Moreover,LS GABAergic neurons extended their functional neural connections via vCA1 glutamatergic pyramidal neurons,and regulating LSGABA→vCA1Gluneural projections affected social behaviors,which were impeded by suppressing LSprojecting vCA1 neuronal activity or inhibiting GABAAreceptors in vCA1.These findings support the hypothesis that LS inputs to the vCA1 can control social prefe rences and social novelty behaviors.These findings provide new insights rega rding the neural circuits that regulate sociability.展开更多
In order to solve the problem of low accuracy of construction project duration prediction, this paper proposes a CNN attention BP combination model </span><span style="font-family:"white-space:...In order to solve the problem of low accuracy of construction project duration prediction, this paper proposes a CNN attention BP combination model </span><span style="font-family:"white-space:normal;">project risk prediction model based on attention mechanism, one-dimensional </span><span style="font-family:"white-space:normal;">convolutional neural network (1d-cnn) and BP neural network. Firstly, the literature analysis method is used to select the risk evaluation index value of construction project, and the attention mechanism is used to determine the weight of risk factors on construction period prediction;then, BP neural network is used to predict the project duration, and accuracy, cross entropy loss function and F1 score are selected to comprehensively evaluate the performance of 1d-cnn-attention-bp combined model. The experimental results show that the duration risk prediction accuracy of the risk prediction model proposed in this paper is more than 90%, which can meet the risk prediction of construction projects with high accuracy.展开更多
以蛹虫草子实体为材料,经水提醇沉后获得沉淀样品 P 和上清样品 L,初步抗 HIV-1蛋白酶检测显示活性成份集中在样品 L 中.进一步利用大孔吸附树脂和高压液相对 L 进行分离纯化,并对纯化样品的体外抗HIV-1蛋白酶活性和抗 HIV-1逆转录酶活...以蛹虫草子实体为材料,经水提醇沉后获得沉淀样品 P 和上清样品 L,初步抗 HIV-1蛋白酶检测显示活性成份集中在样品 L 中.进一步利用大孔吸附树脂和高压液相对 L 进行分离纯化,并对纯化样品的体外抗HIV-1蛋白酶活性和抗 HIV-1逆转录酶活性进行了测定.结果显示,L 经大孔吸附树脂和高压液相分离纯化后得到三种物质均具有抗 HIV-1蛋白酶活性 f1,f2和 f3,其中 f1对 HIV-1蛋白酶抑制作用最强,比 L 提高了约29%;三种物质对 HIV-1逆转录酶均有一定的抑制作用.展开更多
基金supported by the National Natural Science Foundation of China(No.82171599 and No.32270901)the National Key Research and Developmental Program of China(2022YFC2702601 and 2022YFA0806303)the Global Select Project(DJKLX-2022010)of the Institute of Health and Medicine,Hefei Comprehensive National Science Center.
文摘Male infertility can result from impaired sperm motility caused by multiple morphological abnormalities of the flagella(MMAF).Distinct projections encircling the central microtubules of the spermatozoal axoneme play pivotal roles in flagellar bending and spermatozoal movement.Mammalian sperm-associated antigen 17(SPAG17)encodes a conserved axonemal protein of cilia and flagella,forming part of the C1a projection of the central apparatus,with functions related to ciliary/flagellar motility,skeletal growth,and male fertility.This study investigated two novel homozygous SPAG17 mutations(M1:NM_206996.2,c.829+1G>T,p.Asp212_Glu276del;and M2:c.2120del,p.Leu707*)identified in four infertile patients from two consanguineous Pakistani families.These patients displayed the MMAF phenotype confirmed by Papanicolaou staining and scanning electron microscopy assays of spermatozoa.Quantitative real-time polymerase chain reaction(PCR)of patients’spermatozoa also revealed a significant decrease in SPAG17 mRNA expression,and immunofluorescence staining showed the absence of SPAG17 protein signals along the flagella.However,no apparent ciliary-related symptoms or skeletal malformations were observed in the chest X-rays of any of the patients.Transmission electron microscopy of axoneme cross-sections from the patients showed incomplete C1a projection and a higher frequency of missing microtubule doublets 1 and 9 compared with those from fertile controls.Immunofluorescence staining and Western blot analyses of spermatogenesis-associated protein 17(SPATA17),a component of the C1a projection,and sperm-associated antigen 6(SPAG6),a marker of the spring layer,revealed disrupted expression of both proteins in the patients’spermatozoa.Altogether,these findings demonstrated that SPAG17 maintains the integrity of spermatozoal flagellar axoneme,expanding the phenotypic spectrum of SPAG17 mutations in humans.
基金Supported by the Science Research Foundation of Zhanjiang Normal University(L0803)
文摘With the help of the variable-coefficient generalized projected Ricatti equation expansion method, we present exact solutions for the generalized （2＋1）-dimensional nonlinear SchrSdinger equation with variable coefficients. These solutions include solitary wave solutions, soliton-like solutions and trigonometric function solutions. Among these solutions, some are found for the first time.
基金Supported by the Natural Science Foundation of Zhejiang Province under Grant Nos.Y604106, Y606128the Scientific Research Fund of Zhejiang Provincial Education Department of China under Grant No.20070568the Natural Science Foundation of Zhejiang Lishui University under Grant No.KY08003
文摘With a new projective equation, a series of solutions of the （2-J-1）-dimensional dispersive long-water wave system （LWW） is derived. Based on the derived solitary wave solution, we obtain some special fractal localized structures and chaotic patterns.
基金supported by the National Natural Science Foundation of China,No.82171521(to CL)the Special Funds ofTaishan Scholars Project of Shandong Province,No.tsqn202211368(to CL)+2 种基金the Natural Science Foundation of Shandong Province,Nos.ZR2022YQ65(to CL),ZR2021MH073(to CL),ZR2019PH109(to WW)the Projects of Medical and Health Technology Development Program in Shandong Province,China,Nos.202003090720(to DZ),202003070728(to JL),2019 WS329(to DW)the Scientific Research Foundation of Binzhou Medical University,No.BY2018KJ21(to DW)。
文摘Social dysfunction is a risk factor for several neuropsychiatric illnesses.Previous studies have shown that the lateral septum(LS)-related pathway plays a critical role in mediating social behaviors.Howeve r,the role of the connections between the LS and its downstream brain regions in social behavio rs remains unclea r.In this study,we conducted a three-chamber test using electrophysiological and chemogenetic approaches in mice to determine how LS projections to ventral CA1(vCA1)influence sociability.Our res ults showed that gamma-aminobutyric acid(GABA)-e rgic neuro ns were activated following social experience,and that social behavio rs were enhanced by chemogenetic modulation of these neurons.Moreover,LS GABAergic neurons extended their functional neural connections via vCA1 glutamatergic pyramidal neurons,and regulating LSGABA→vCA1Gluneural projections affected social behaviors,which were impeded by suppressing LSprojecting vCA1 neuronal activity or inhibiting GABAAreceptors in vCA1.These findings support the hypothesis that LS inputs to the vCA1 can control social prefe rences and social novelty behaviors.These findings provide new insights rega rding the neural circuits that regulate sociability.
文摘In order to solve the problem of low accuracy of construction project duration prediction, this paper proposes a CNN attention BP combination model </span><span style="font-family:"white-space:normal;">project risk prediction model based on attention mechanism, one-dimensional </span><span style="font-family:"white-space:normal;">convolutional neural network (1d-cnn) and BP neural network. Firstly, the literature analysis method is used to select the risk evaluation index value of construction project, and the attention mechanism is used to determine the weight of risk factors on construction period prediction;then, BP neural network is used to predict the project duration, and accuracy, cross entropy loss function and F1 score are selected to comprehensively evaluate the performance of 1d-cnn-attention-bp combined model. The experimental results show that the duration risk prediction accuracy of the risk prediction model proposed in this paper is more than 90%, which can meet the risk prediction of construction projects with high accuracy.
文摘以蛹虫草子实体为材料,经水提醇沉后获得沉淀样品 P 和上清样品 L,初步抗 HIV-1蛋白酶检测显示活性成份集中在样品 L 中.进一步利用大孔吸附树脂和高压液相对 L 进行分离纯化,并对纯化样品的体外抗HIV-1蛋白酶活性和抗 HIV-1逆转录酶活性进行了测定.结果显示,L 经大孔吸附树脂和高压液相分离纯化后得到三种物质均具有抗 HIV-1蛋白酶活性 f1,f2和 f3,其中 f1对 HIV-1蛋白酶抑制作用最强,比 L 提高了约29%;三种物质对 HIV-1逆转录酶均有一定的抑制作用.
