Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupli...Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupling(ARC)microbial inoculants and found it can reduce A.flavus abundance in the soil and promote efficient nodulation in peanuts.However,the impact of ARC microbial inoculants on different resistant varieties of A.flavus remains unclear.In this study,we screened peanut varieties that were resistant and susceptible to A.flavus and evaluated their nodulation ability and growth performance after ARC microbial inoculants treatment in the field.The results demonstrated that the nodule number and nitrogenase activity of both varieties significantly increased after ARC microbial inoculants treatment,with the highly susceptible variety AH24 showing a greater increase.For photosynthetic parameters,both varieties also increased after ARC microbial inoculants treatment,but the increase was greater in the moderately resistant variety AH1 than in the highly susceptible variety AH24.Finally,we found that the yield and yield-related traits of the moderately resistant variety AH1 were better than those of the highly susceptible variety AH24.After ARC microbial inoculants treatment,the yield traits of both peanut varieties still increased significantly,but the degree of increase of the moderately resistant variety AH1 was smaller than that of the highly susceptible variety AH24.In addition,the abundance of A.flavus in the rhizosphere soil of the two varieties significantly decreased after ARC microbial inoculants treatment,with no significant difference between the varieties.These results indicated that ARC microbial inoculants exert differential effects on the nodulation and growth of different resistant peanut varieties and have a better effect on highly susceptible varieties.These results provide a solid theoretical basis for the efficient use of ARC microbial inoculants in the field of peanuts in the future.展开更多
The microbial agent ARC-BBBE demonstration trials were conducted in four provinces in the main peanutproducing areas of the Huang-huai-hai plain of China.The results revealed that the application of ARC-BBBE led to a ...The microbial agent ARC-BBBE demonstration trials were conducted in four provinces in the main peanutproducing areas of the Huang-huai-hai plain of China.The results revealed that the application of ARC-BBBE led to a 1.09–1.70 fold increase in the number of nodules in the treatment group at the demonstration site compared to the control group.Moreover,the nodule weight in the treatment group was 0.80–3.32 times higher than that of the control group,and nitrogenase activity per plant showed a significant enhancement by 1.00–2.83 fold compared to controls.Additionally,notable improvements were observed in terms of increased fresh weight of whole plants,well-filled pod numbers,and enhanced growth performance;ultimately resulting in a harvest yield increase ranging from 9.46%to 49.04%.The abundance of Aspergillus flavus in rhizosphere soil was determined by the dilution spread plate method,and the inhibition rate was up to 86.7%.The application of ARC-BBBE in the significant peanut-producing areas of Huang-huai-hai has effects of promoting growth,nodulation,and increasing production.At the same time,it has the effect of inhibiting and controlling soil Aspergillus flavus,which provides a new green and low-carbon way to promote the high-quality development of the peanut industry.展开更多
Morphology,biomass,nitrate reductase(NR)and nitrogenase activity in Albizia chinensis(Osb.)Merr.nodules were assessed on monthly and seasonal basis for 1 year.Average NR and nitrogenase activity was higher during the ...Morphology,biomass,nitrate reductase(NR)and nitrogenase activity in Albizia chinensis(Osb.)Merr.nodules were assessed on monthly and seasonal basis for 1 year.Average NR and nitrogenase activity was higher during the rainy season,reaching a maximum in August.Thereafter,activity decreased through autumn and reached a minimum value during winter.Fresh and dry biomass of nodules increased gradually from summer to the rainy season and then started decreasing with the onset of winter as nodules began to senesce.Among four developmental stages of the nodules that correspond to their increasing age,NR and nitrogenase activity remained low in stage 1 nodules and peaked in stage 2.The activity of both enzymes further decreased with increasing age(stage 2 to stage 4).Morphological features such as shape,diameter and color varied considerably among the developmental stages.Stage 1 nodules were cream-colored,oval to heartshaped with smallest average diameter whereas at stage 2,they became bilobed to tetralobed.On the other hand,stage 3 nodules had the largest average diameter and were multilobed in structure.Stage 4 nodules that correspond to the senescing stage were dark brown to black,multilobed,flattened and hollow due to degeneration of nodular tissue.展开更多
Dear Editor,Biological soil crusts(BSCs),a layered structure formed by associations of soil organisms and topsoil,dominate arid and semiarid areas and serve important ecological functions in these areas(Eldridge and G...Dear Editor,Biological soil crusts(BSCs),a layered structure formed by associations of soil organisms and topsoil,dominate arid and semiarid areas and serve important ecological functions in these areas(Eldridge and Greene,1994).Nitrogen fixation by BSCs is the main source of N in arid and semi-arid ecosystems.Desiccation is the most notable factor that influences BSCs,which recover physiological activity only after moistening.By influencing the amount of carbohydrates,展开更多
基金supported by the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202416)the Foundation of Hubei Hongshan Laboratory(2021hszd015)+1 种基金the Science and Technology Major Projects of Hubei Province(2023BBA002)the Knowledge Innovation Program of Wuhan-Basi Research(2023020201010126)。
文摘Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil,leading to serious economic losses.Previously,our research team developed the Aspergillus-Rihizobia coupling(ARC)microbial inoculants and found it can reduce A.flavus abundance in the soil and promote efficient nodulation in peanuts.However,the impact of ARC microbial inoculants on different resistant varieties of A.flavus remains unclear.In this study,we screened peanut varieties that were resistant and susceptible to A.flavus and evaluated their nodulation ability and growth performance after ARC microbial inoculants treatment in the field.The results demonstrated that the nodule number and nitrogenase activity of both varieties significantly increased after ARC microbial inoculants treatment,with the highly susceptible variety AH24 showing a greater increase.For photosynthetic parameters,both varieties also increased after ARC microbial inoculants treatment,but the increase was greater in the moderately resistant variety AH1 than in the highly susceptible variety AH24.Finally,we found that the yield and yield-related traits of the moderately resistant variety AH1 were better than those of the highly susceptible variety AH24.After ARC microbial inoculants treatment,the yield traits of both peanut varieties still increased significantly,but the degree of increase of the moderately resistant variety AH1 was smaller than that of the highly susceptible variety AH24.In addition,the abundance of A.flavus in the rhizosphere soil of the two varieties significantly decreased after ARC microbial inoculants treatment,with no significant difference between the varieties.These results indicated that ARC microbial inoculants exert differential effects on the nodulation and growth of different resistant peanut varieties and have a better effect on highly susceptible varieties.These results provide a solid theoretical basis for the efficient use of ARC microbial inoculants in the field of peanuts in the future.
基金The authors thank the National Natural Sciences Foundation of China(32030085,U22A20551)Major project of Hubei Hongshan Laboratory(2021hszd015)+1 种基金Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP2021-OCRI)Knowledge Innovation Program of Wuhan-Shuguang Project(3570).
文摘The microbial agent ARC-BBBE demonstration trials were conducted in four provinces in the main peanutproducing areas of the Huang-huai-hai plain of China.The results revealed that the application of ARC-BBBE led to a 1.09–1.70 fold increase in the number of nodules in the treatment group at the demonstration site compared to the control group.Moreover,the nodule weight in the treatment group was 0.80–3.32 times higher than that of the control group,and nitrogenase activity per plant showed a significant enhancement by 1.00–2.83 fold compared to controls.Additionally,notable improvements were observed in terms of increased fresh weight of whole plants,well-filled pod numbers,and enhanced growth performance;ultimately resulting in a harvest yield increase ranging from 9.46%to 49.04%.The abundance of Aspergillus flavus in rhizosphere soil was determined by the dilution spread plate method,and the inhibition rate was up to 86.7%.The application of ARC-BBBE in the significant peanut-producing areas of Huang-huai-hai has effects of promoting growth,nodulation,and increasing production.At the same time,it has the effect of inhibiting and controlling soil Aspergillus flavus,which provides a new green and low-carbon way to promote the high-quality development of the peanut industry.
文摘Morphology,biomass,nitrate reductase(NR)and nitrogenase activity in Albizia chinensis(Osb.)Merr.nodules were assessed on monthly and seasonal basis for 1 year.Average NR and nitrogenase activity was higher during the rainy season,reaching a maximum in August.Thereafter,activity decreased through autumn and reached a minimum value during winter.Fresh and dry biomass of nodules increased gradually from summer to the rainy season and then started decreasing with the onset of winter as nodules began to senesce.Among four developmental stages of the nodules that correspond to their increasing age,NR and nitrogenase activity remained low in stage 1 nodules and peaked in stage 2.The activity of both enzymes further decreased with increasing age(stage 2 to stage 4).Morphological features such as shape,diameter and color varied considerably among the developmental stages.Stage 1 nodules were cream-colored,oval to heartshaped with smallest average diameter whereas at stage 2,they became bilobed to tetralobed.On the other hand,stage 3 nodules had the largest average diameter and were multilobed in structure.Stage 4 nodules that correspond to the senescing stage were dark brown to black,multilobed,flattened and hollow due to degeneration of nodular tissue.
基金supported by the Special Fund for Forest Scientific Research in the Public Welfare of China (201404204-02)
文摘Dear Editor,Biological soil crusts(BSCs),a layered structure formed by associations of soil organisms and topsoil,dominate arid and semiarid areas and serve important ecological functions in these areas(Eldridge and Greene,1994).Nitrogen fixation by BSCs is the main source of N in arid and semi-arid ecosystems.Desiccation is the most notable factor that influences BSCs,which recover physiological activity only after moistening.By influencing the amount of carbohydrates,
