The adsorption of nitric oxide adsorption on clean Mod(111), Mo(111)/O2-(4×4), Mo(111)/O2-(1×3), Mo(111)/O2-(112) facets and Mo(111) oxide surfaces was studied. Mass 28 (N2) desorption spectra show two high ...The adsorption of nitric oxide adsorption on clean Mod(111), Mo(111)/O2-(4×4), Mo(111)/O2-(1×3), Mo(111)/O2-(112) facets and Mo(111) oxide surfaces was studied. Mass 28 (N2) desorption spectra show two high temperature peaks at approximate 1030 (β1) and 1200K (β2). For NO exposures less than 0. 1 L only the ft peak was observed. At higher exposures, the ft peak appeared and small amounts of N2O as well as NO desorbed. The preadsorption of oxygen blocked the β2 desorption partially on the (4×4) and (1×3) surfaces and fully on the (112) facet and oxide surfaces. In addition, the β1 desorption peak shifted to higher temperatures with increasing oxygen preexposure.展开更多
BACKGROUND：Studies have reported that nitric oxide synthase （NOS） inhibitor can prolong the latency of hyperbaric oxygen-induced convulsion （HBOC）. However, there are very few reports addressing the influence of ...BACKGROUND：Studies have reported that nitric oxide synthase （NOS） inhibitor can prolong the latency of hyperbaric oxygen-induced convulsion （HBOC）. However, there are very few reports addressing the influence of NOS inhibitor on mental behavior. OBJECTIVE： To investigate behavioral changes after HBOC in gerbils, as well as the influence of NOS inhibitor. DESIGN, TIME AND SETTING： Randomized experiments were performed in the Laboratory of Hyperbaric Pressure and Diving Physiology, Naval Medical Research Institute of Chinese PLA （Shanghai, China） from March 2005 to June 2007. MATERIALS： Forty male gerbils were randomly divided into five groups： HBOC, saline control, NOS inhibitor, pressure control, and normal control. Each group contained eight animals. METHODS： In the HBOC group, once depression induction ended, animals were removed from the chamber five minutes after the first appearance of generalized convulsion induced by 0.5 MPa hyperbaric oxygen. Ten minutes before entering the chamber, saline control and NOS inhibitor animals were intraperitoneally injected with 1 mL saline and 20 mg/kg NG-nitro-L-arginine, respectively. The pressure control group was only exposed to 0.5 MPa. The remaining procedures in these three groups were identical to the HBOC group. The normal control group received no intervention. MAIN OUTCOME MEASURES： Open field test scores in gerbils prior to HBOC, as well as immediately, 24 hours, and 72 hours after decompression ended. RESULTS： HBOC was not detected in either the normal control or the pressure control group, and there were no significant differences in open field test scores prior to and after HBOC （P 〉 0.05）. HBOC occurred in the HBOC, saline control, and NOS inhibitor groups, with significant differences in open field test scores after decompression ended compared to normal control and pressure control groups （P 〈 0.05–0.01）. Compared to the HBOC and saline control groups, the NOS inhibitor group exhibited a significantly lower score in the open field test immediately after decompression, and a higher score at 24 and 72 hours （P 〈 0.05–0.01）. CONCLUSION： NOS inhibitor can regulate behavioral changes in gerbils after HBOC.展开更多
Nitrogen dioxide（NO2） is one of the most common and harmful air pollutants. To analyze the response of plants to NO2 stress, we investigated the morphological change, reactive oxygen species（ROS） production and an...Nitrogen dioxide（NO2） is one of the most common and harmful air pollutants. To analyze the response of plants to NO2 stress, we investigated the morphological change, reactive oxygen species（ROS） production and antioxidant enzyme activity in Arabidopsis thaliana（Col-0） exposed to 1.7, 4, 8.5, and 18.8 mg/m3NO2. The results indicate that NO2 exposure affected plant growth and chlorophyll（Chl） content, and increased oxygen free radical（O2-）production rate in Arabidopsis shoots. Furthermore, NO2 elevated the levels of lipid peroxidation and protein oxidation, accompanied by the induction of antioxidant enzyme activities and change of ascorbate（As A） and glutathione（GSH） contents. Following this, we mimicked nitric acid mist under experimental conditions, and confirmed the antioxidant mechanism of the plant to the stress. Our results imply that NO2 and its acid mist caused pollution risk to plant systems. During the process, increased ROS acted as a signal to induce a defense response, and antioxidant status played an important role in plant protection against NO2/nitric acid mist-caused oxidative damage.展开更多
AIM:To investigate the effect of prednisolone,a synthetic glucocorticoid used in inflammatory diseases,on the growth of cultured osteosarcoma cells.METHODS:Two osteosarcoma cell lines with different degree of differen...AIM:To investigate the effect of prednisolone,a synthetic glucocorticoid used in inflammatory diseases,on the growth of cultured osteosarcoma cells.METHODS:Two osteosarcoma cell lines with different degree of differentiation were used.SaOS2 show a rather mature phenotype,while U2 OS are negative for almost all osteoblastic markers.The cells were exposed to different concentrations of prednisolone(1-9 μmol/L) with or without antioxidants or the inhibitor of inducible nitric oxide synthase(i NOS) l-N6-(iminoethyl)-lysine-HCl(L-NIL).Cell growth was assessed by counting viable cells.The production of nitric oxide(NO) was measured in the conditioned media by the Griess method.The production of reactive oxygen species was quantified using 2'-7'-dichlorofluorescein diacetate.Western blot with specific antibodies against NOSs was performed on cell extracts.RESULTS:Prednisolone inhibited SaOS2 cell growth in a dose dependent manner.No significant effects were observed in U2OS.The inhibition of SaOS2 growth is not due to oxidative stress,because antioxidants do not rescue cell proliferation.Since high concentrations of NO inhibit bone formation,we also measured NO and found it induced in SaOS2,but not in U2 OS,exposed to prednisolone,because of the upregulation of i NOS as detected by western blot.Therefore,we treated SaOS2 with prednisolone in the presence or in the absence of L-NIL.L-NIL prevented NO release induced by prednisolone at all the concentrations apart from 9 μmol/L.At the same concentrations,we found that L-NIL rescued SaOS2 growth after exposure to prednisolone.In U2 OS cells,prednisolone did not induce NO production nor affected cell growth.All together,these data indicate that a link exists between increased amounts of NO and growth inhibition in response to prednisolone in SaOS2.CONCLUSION:Prednisolone inhibited SaOS2 proliferation by increasing the release of NO through the upregulation of i NOS,while no effect was exerted on U2OS.展开更多
Objective: To observe the effect of acupuncture on blood oxygen free radical (OFR) and nitric oxide (NO) levels in the treatment of apoplectic sequelae. Methods: A total of 61 cases of apoplectic patients were subject...Objective: To observe the effect of acupuncture on blood oxygen free radical (OFR) and nitric oxide (NO) levels in the treatment of apoplectic sequelae. Methods: A total of 61 cases of apoplectic patients were subjected into this study and randomly divided into "JIN San Zhen" group (n=30) and control group (n=31). Blood lipid peroxidase (LPO), superoxide dismutase (SOD), glutathione peroxidase(GSH Px) and nitric oxide (NO) contents before and after acupuncture treatment were determined with radioimmunoassay. In both groups, acupuncture was given once daily, six times a week, with 4 weeks being a therapeutic course and with the interval between two weeks being a week, 3 courses all together. In "JIN San Zhen" group, acupoints of "JIN San Zhen" were used predominately, while in control group, scalp point Motor Sensory Area (MS 8) was used as the main point. Results: Self comparison showed that after 3 courses of treatment, in both groups, LPO and NO levels decreased significantly (P<0.05-0.01), SOD and GST Px values increased considerably (P<0.05-0.01). Comparison between two groups indicated that the effects of "JIN San Zhen" group are significantly superior to those of control group in raising blood SOD and GST Px levels (P<0.05-0.01) and in lowering blood NO content (P<0.01). Analysis on the correlation between the restoration of neural function and the changes of LPO, SOD and GST Px levels suggested that the effect of acupuncture in improving neural function may be related to changes of the aforementioned indexes. Conclusion: Acupuncture therapy can significantly lower blood LPO and NO levels and evidently raise blood SOD and GST Px levels in stroke patients.展开更多
目的:评估吸入一氧化氮(inhaled nitric oxide,INO)联合高频振荡通气(high frequency oscillatory ventilation,HFOV)治疗中重度新生儿持续肺动脉高压(persistent pulmonary hypertension of the newborn,PPHN)的疗效及安全性。方法:将...目的:评估吸入一氧化氮(inhaled nitric oxide,INO)联合高频振荡通气(high frequency oscillatory ventilation,HFOV)治疗中重度新生儿持续肺动脉高压(persistent pulmonary hypertension of the newborn,PPHN)的疗效及安全性。方法:将商丘市第一人民医院新生儿重症监护室收治的104例中重度PPHN患儿随机分为研究组(INO+HFOV,n=52)与对照组(INO+常规机械通气,n=52),比较两组干预后24 h及48 h的氧合指数(oxygenation index,OI)、右心室收缩压/体循环收缩压比值(right ventricular systolic pressure/systemic systolic blood pressure,RVSP/SBP)、左心室输出量(left ventricular output,LVO)、药物代谢指标(高铁血红蛋白、血小板)及通气并发症(气胸、脑室内出血)。结果:研究组干预后24 h及48 h OI(18.25±4.30 vs 23.75±5.20;12.45±3.15 vs 17.85±4.25)与RVSP/SBP比值(0.45±0.09 vs 0.52±0.10;0.38±0.07 vs0.44±0.08)均显著低于对照组(P<0.001),LVO(162.75±25.15 mL/kg vs 145.30±22.60 mL/kg;178.45±28.50 mL/kg vs 155.80±24.75 mL/kg)显著高于对照组(P<0.001)。研究组气胸发生率更低(1.92%vs 15.38%,P=0.036),两组高铁血红蛋白水平无差异,但研究组血小板计数显著低于对照组(P=0.031)。结论:INO联合HFOV可快速改善中重度PPHN患儿的氧合与血流动力学,降低肺动脉压力及气胸风险,安全性可控,为传统INO联合常规通气疗效不足的患儿提供了优化方案。展开更多
文摘The adsorption of nitric oxide adsorption on clean Mod(111), Mo(111)/O2-(4×4), Mo(111)/O2-(1×3), Mo(111)/O2-(112) facets and Mo(111) oxide surfaces was studied. Mass 28 (N2) desorption spectra show two high temperature peaks at approximate 1030 (β1) and 1200K (β2). For NO exposures less than 0. 1 L only the ft peak was observed. At higher exposures, the ft peak appeared and small amounts of N2O as well as NO desorbed. The preadsorption of oxygen blocked the β2 desorption partially on the (4×4) and (1×3) surfaces and fully on the (112) facet and oxide surfaces. In addition, the β1 desorption peak shifted to higher temperatures with increasing oxygen preexposure.
文摘BACKGROUND：Studies have reported that nitric oxide synthase （NOS） inhibitor can prolong the latency of hyperbaric oxygen-induced convulsion （HBOC）. However, there are very few reports addressing the influence of NOS inhibitor on mental behavior. OBJECTIVE： To investigate behavioral changes after HBOC in gerbils, as well as the influence of NOS inhibitor. DESIGN, TIME AND SETTING： Randomized experiments were performed in the Laboratory of Hyperbaric Pressure and Diving Physiology, Naval Medical Research Institute of Chinese PLA （Shanghai, China） from March 2005 to June 2007. MATERIALS： Forty male gerbils were randomly divided into five groups： HBOC, saline control, NOS inhibitor, pressure control, and normal control. Each group contained eight animals. METHODS： In the HBOC group, once depression induction ended, animals were removed from the chamber five minutes after the first appearance of generalized convulsion induced by 0.5 MPa hyperbaric oxygen. Ten minutes before entering the chamber, saline control and NOS inhibitor animals were intraperitoneally injected with 1 mL saline and 20 mg/kg NG-nitro-L-arginine, respectively. The pressure control group was only exposed to 0.5 MPa. The remaining procedures in these three groups were identical to the HBOC group. The normal control group received no intervention. MAIN OUTCOME MEASURES： Open field test scores in gerbils prior to HBOC, as well as immediately, 24 hours, and 72 hours after decompression ended. RESULTS： HBOC was not detected in either the normal control or the pressure control group, and there were no significant differences in open field test scores prior to and after HBOC （P 〉 0.05）. HBOC occurred in the HBOC, saline control, and NOS inhibitor groups, with significant differences in open field test scores after decompression ended compared to normal control and pressure control groups （P 〈 0.05–0.01）. Compared to the HBOC and saline control groups, the NOS inhibitor group exhibited a significantly lower score in the open field test immediately after decompression, and a higher score at 24 and 72 hours （P 〈 0.05–0.01）. CONCLUSION： NOS inhibitor can regulate behavioral changes in gerbils after HBOC.
基金supported by the National Natural Science Foundation of China (Nos.21477070, 21377076)the Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) (Nos.20121401110003, 20131401110005)+2 种基金the Project for Science and Technology Development of Shanxi Province (No.20120313009-2)the Research Project supported by the Shanxi Scholarship Council of China (No.2012-009)the Program for the Top Young and Middle aged Innovative Talents of Higher Learning Institutions of Shanxi (TYMIT, No.20120201)
文摘Nitrogen dioxide（NO2） is one of the most common and harmful air pollutants. To analyze the response of plants to NO2 stress, we investigated the morphological change, reactive oxygen species（ROS） production and antioxidant enzyme activity in Arabidopsis thaliana（Col-0） exposed to 1.7, 4, 8.5, and 18.8 mg/m3NO2. The results indicate that NO2 exposure affected plant growth and chlorophyll（Chl） content, and increased oxygen free radical（O2-）production rate in Arabidopsis shoots. Furthermore, NO2 elevated the levels of lipid peroxidation and protein oxidation, accompanied by the induction of antioxidant enzyme activities and change of ascorbate（As A） and glutathione（GSH） contents. Following this, we mimicked nitric acid mist under experimental conditions, and confirmed the antioxidant mechanism of the plant to the stress. Our results imply that NO2 and its acid mist caused pollution risk to plant systems. During the process, increased ROS acted as a signal to induce a defense response, and antioxidant status played an important role in plant protection against NO2/nitric acid mist-caused oxidative damage.
文摘AIM:To investigate the effect of prednisolone,a synthetic glucocorticoid used in inflammatory diseases,on the growth of cultured osteosarcoma cells.METHODS:Two osteosarcoma cell lines with different degree of differentiation were used.SaOS2 show a rather mature phenotype,while U2 OS are negative for almost all osteoblastic markers.The cells were exposed to different concentrations of prednisolone(1-9 μmol/L) with or without antioxidants or the inhibitor of inducible nitric oxide synthase(i NOS) l-N6-(iminoethyl)-lysine-HCl(L-NIL).Cell growth was assessed by counting viable cells.The production of nitric oxide(NO) was measured in the conditioned media by the Griess method.The production of reactive oxygen species was quantified using 2'-7'-dichlorofluorescein diacetate.Western blot with specific antibodies against NOSs was performed on cell extracts.RESULTS:Prednisolone inhibited SaOS2 cell growth in a dose dependent manner.No significant effects were observed in U2OS.The inhibition of SaOS2 growth is not due to oxidative stress,because antioxidants do not rescue cell proliferation.Since high concentrations of NO inhibit bone formation,we also measured NO and found it induced in SaOS2,but not in U2 OS,exposed to prednisolone,because of the upregulation of i NOS as detected by western blot.Therefore,we treated SaOS2 with prednisolone in the presence or in the absence of L-NIL.L-NIL prevented NO release induced by prednisolone at all the concentrations apart from 9 μmol/L.At the same concentrations,we found that L-NIL rescued SaOS2 growth after exposure to prednisolone.In U2 OS cells,prednisolone did not induce NO production nor affected cell growth.All together,these data indicate that a link exists between increased amounts of NO and growth inhibition in response to prednisolone in SaOS2.CONCLUSION:Prednisolone inhibited SaOS2 proliferation by increasing the release of NO through the upregulation of i NOS,while no effect was exerted on U2OS.
文摘Objective: To observe the effect of acupuncture on blood oxygen free radical (OFR) and nitric oxide (NO) levels in the treatment of apoplectic sequelae. Methods: A total of 61 cases of apoplectic patients were subjected into this study and randomly divided into "JIN San Zhen" group (n=30) and control group (n=31). Blood lipid peroxidase (LPO), superoxide dismutase (SOD), glutathione peroxidase(GSH Px) and nitric oxide (NO) contents before and after acupuncture treatment were determined with radioimmunoassay. In both groups, acupuncture was given once daily, six times a week, with 4 weeks being a therapeutic course and with the interval between two weeks being a week, 3 courses all together. In "JIN San Zhen" group, acupoints of "JIN San Zhen" were used predominately, while in control group, scalp point Motor Sensory Area (MS 8) was used as the main point. Results: Self comparison showed that after 3 courses of treatment, in both groups, LPO and NO levels decreased significantly (P<0.05-0.01), SOD and GST Px values increased considerably (P<0.05-0.01). Comparison between two groups indicated that the effects of "JIN San Zhen" group are significantly superior to those of control group in raising blood SOD and GST Px levels (P<0.05-0.01) and in lowering blood NO content (P<0.01). Analysis on the correlation between the restoration of neural function and the changes of LPO, SOD and GST Px levels suggested that the effect of acupuncture in improving neural function may be related to changes of the aforementioned indexes. Conclusion: Acupuncture therapy can significantly lower blood LPO and NO levels and evidently raise blood SOD and GST Px levels in stroke patients.
文摘目的:评估吸入一氧化氮(inhaled nitric oxide,INO)联合高频振荡通气(high frequency oscillatory ventilation,HFOV)治疗中重度新生儿持续肺动脉高压(persistent pulmonary hypertension of the newborn,PPHN)的疗效及安全性。方法:将商丘市第一人民医院新生儿重症监护室收治的104例中重度PPHN患儿随机分为研究组(INO+HFOV,n=52)与对照组(INO+常规机械通气,n=52),比较两组干预后24 h及48 h的氧合指数(oxygenation index,OI)、右心室收缩压/体循环收缩压比值(right ventricular systolic pressure/systemic systolic blood pressure,RVSP/SBP)、左心室输出量(left ventricular output,LVO)、药物代谢指标(高铁血红蛋白、血小板)及通气并发症(气胸、脑室内出血)。结果:研究组干预后24 h及48 h OI(18.25±4.30 vs 23.75±5.20;12.45±3.15 vs 17.85±4.25)与RVSP/SBP比值(0.45±0.09 vs 0.52±0.10;0.38±0.07 vs0.44±0.08)均显著低于对照组(P<0.001),LVO(162.75±25.15 mL/kg vs 145.30±22.60 mL/kg;178.45±28.50 mL/kg vs 155.80±24.75 mL/kg)显著高于对照组(P<0.001)。研究组气胸发生率更低(1.92%vs 15.38%,P=0.036),两组高铁血红蛋白水平无差异,但研究组血小板计数显著低于对照组(P=0.031)。结论:INO联合HFOV可快速改善中重度PPHN患儿的氧合与血流动力学,降低肺动脉压力及气胸风险,安全性可控,为传统INO联合常规通气疗效不足的患儿提供了优化方案。
