In order to</span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> study the meat quality characteristics of </span>...In order to</span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> study the meat quality characteristics of </span><span style="font-family:Verdana;">Ningdu</span><span style="font-family:Verdana;"> yellow rooster, the 7 indexes of 120 healthy </span><span style="font-family:Verdana;">Ningdu</span><span style="font-family:Verdana;"> yellow rooster</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> after </span><span style="font-family:Verdana;">slaughter</span><span style="font-family:Verdana;"> at the age of 16 weeks were determined, and correlation analysis, regression analysis </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> principal component analysis were carried out among the indexes.</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">The results showed that the variation coefficient of PH value was smaller than that of water drop rate and meat colorimetric, and the lactic acid produced by anaerobic glycolysis of glycogen in chicken meat was slower.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">Through correlation analysis, the 24-hour drip rate was strongly correlated with the 48-hour drip rate (coefficient ≥</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">0.7,</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">p</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">≤</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">0.05), and the other indicators were independent of each other.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">By means of</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> regression analysis, the regression equation (fitting degree R</span><sup><span style="font-family:Verdana;">2</span></sup></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">=</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">0.984) of </span><span style="font-family:Verdana;">24</span><span style="font-family:Verdana;">- and 48-hour drip rate was established.</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">Principal component analysis synthesized 7 indicators into 3 principal components, with a cumulative contribution rate of 70.13%, indicating that the meat quality of Ningdu Yellow chicken rooster is mainly selected by water drop rate, PH value </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> meat color test.展开更多
The Ningdu basin,located in southern Jiangxi province of southwest China,is one of the Mesozoic basin groups which has exploration prospects for geothermal energy.A study on the detailed velocity structure of the Ning...The Ningdu basin,located in southern Jiangxi province of southwest China,is one of the Mesozoic basin groups which has exploration prospects for geothermal energy.A study on the detailed velocity structure of the Ningdu basin can provide important information for geothermal resource exploration.In this study,we deployed a dense seismic array in the Ningdu basin to investigate the 3D velocity structure and discuss implications for geothermal exploration and geological evolution.Based on the dense seismic array including 35 short-period(5 s-100 Hz)seismometers with an average interstation distance of~5 km,Rayleigh surface wave dispersion curves were extracted from the continuous ambient noise data for surface wave tomographic inversion.Group velocity tomography was conducted and the 3D S-wave velocity structure was inverted by the neighborhood algorithm.The results revealed obvious low-velocity anomalies in the center of the basin,consistent with the low-velocity Cretaceous sedimentary rocks.The basement and basin-controlling fault can also be depicted by the S-wave velocity anomalies.The obvious seismic interface is about 2 km depth in the basin center and decreases to 700 m depth near the basin boundary,suggesting spatial thickness variations of the Cretaceous sediment.The fault features of the S-wave velocity profile coincide with the geological cognition of the western boundary basincontrolling fault,which may provide possible upwelling channels for geothermal fluid.This study suggests that seismic tomography with a dense array is an effective method and can play an important role in the detailed investigations of sedimentary basins.展开更多
试验旨在探讨成年宁都黄鸡睾丸的蛋白质表达谱,筛选大小睾丸个体差异表达蛋白和功能通路,为繁殖性能选育提供基础数据。以6只22周龄宁都黄鸡的睾丸组织为研究对象,分为大睾丸组(H-TES)和小睾丸组(L-TES),采用串联质量标签(tandem mass t...试验旨在探讨成年宁都黄鸡睾丸的蛋白质表达谱,筛选大小睾丸个体差异表达蛋白和功能通路,为繁殖性能选育提供基础数据。以6只22周龄宁都黄鸡的睾丸组织为研究对象,分为大睾丸组(H-TES)和小睾丸组(L-TES),采用串联质量标签(tandem mass tags, TMT)技术对2组个体睾丸蛋白质进行差异表达、功能富集和基因互作分析,初步筛选候选蛋白质,分析这些蛋白质在6个个体的表达量与6个睾丸性状指标(左侧睾丸重、右侧睾丸重、总睾丸重、左侧睾丸指数、右侧睾丸指数、总睾丸指数)之间的相关性,分析候选蛋白质的功能。结果表明,宁都黄鸡睾丸中共鉴定到4 683个蛋白质,筛选到144个差异表达蛋白质。GO分析结果表明,差异蛋白质主要参与精子结构组成,具有尿蛋白-谷氨酸连接酶活性、胸腺嘧啶结合、脱氢抗坏血酸跨膜转运体活性等功能。差异蛋白质显著富集的KEGG通路为精氨酸和脯氨酸代谢、泛酸和辅酶A的生物合成、β-丙氨酸代谢等。蛋白质互作网络中有34个差异蛋白质,初步筛选出30个候选蛋白质。结合相关性分析和文献查阅,筛选出16个关键候选蛋白质,即SPAG6、TPPP2、ENKUR、ODF2、SAXO1、TCP11、DNALI1、CCDC63、TSSK3、TEKT2、TEKT3、ALDH2、DNAH5、CCDC40、CCDC39、DNAH10。结合关键候选蛋白质的功能,初步确定精氨酸和脯氨酸代谢、泛酸和辅酶A生物合成、β-丙氨酸代谢、紧密连接、间隙连接是睾丸性状调控关键KEGG通路。研究结果为地方鸡种睾丸质量性状选育提供了基础依据。展开更多
为研究不同养殖方式下宁都黄鸡肌肉关键挥发性风味物质,将试验鸡随机分为笼养组和平养组,饲喂同一日粮。试验鸡达上市日龄时对鸡肉进行感官品尝评价和挥发性风味物质检测,并采用正交偏最小二乘-判别分析(orthogonal partial least squar...为研究不同养殖方式下宁都黄鸡肌肉关键挥发性风味物质,将试验鸡随机分为笼养组和平养组,饲喂同一日粮。试验鸡达上市日龄时对鸡肉进行感官品尝评价和挥发性风味物质检测,并采用正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)方法筛选与不同养殖方式相关的差异性风味物质。结果表明:平养组和笼养组共有的挥发性风味物质27种,主要为酚类、醇类和烃类。挥发性风味物质中,己醛、1-辛烯-3-醇、E-2-壬烯醛、正己醇、壬醛、2,3-戊二酮、癸醛、2,3-辛二酮、E-2-辛烯醛为具有显著性差异的挥发性风味物质。综上,这一研究可为地方鸡肉品质基于风味物质的评价提供科学依据。展开更多
【背景】睾丸是雄性动物重要生殖器官,具有产生精子和分泌雄激素的功能,睾丸质量与繁殖性能有直接联系。睾丸性状受下丘脑-垂体-性腺轴多基因共同调控,其分子机理尚不清楚。宁都黄鸡睾丸性状变异系数大,繁殖性能一致性差。【目的】通过...【背景】睾丸是雄性动物重要生殖器官,具有产生精子和分泌雄激素的功能,睾丸质量与繁殖性能有直接联系。睾丸性状受下丘脑-垂体-性腺轴多基因共同调控,其分子机理尚不清楚。宁都黄鸡睾丸性状变异系数大,繁殖性能一致性差。【目的】通过对不同睾丸性状个体垂体组织进行全长转录组测序,挖掘候选基因以及关键信号通路,探究影响睾丸性状的遗传基础,以期为发展地方鸡种睾丸性状的选种选育提供依据。【方法】以70只22周龄宁都黄公鸡为研究对象,分为大睾丸组和小睾丸组,从两组中分别选取3个个体,构建垂体组织的全长转录本表达谱,筛选高表达量及两组个体差异表达基因或转录本,对差异基因或转录本进行功能富集分析。随机选取6个基因,进行基因表达水平验证。参考已知垂体细胞的标记基因,筛选与8种细胞标记基因对应的差异表达基因或转录本,并分析这些差异基因或转录本以及高表达量基因或转录本与睾丸性状相关性。【结果】在6个宁都黄鸡垂体组织中,检测到21834个基因,29355个全长转录本。其中,332个基因及229个转录本在两组个体中差异表达。KEGG通路分析结果显示,这些差异基因或转录本主要富集于Apelin信号、甘油磷脂代谢等通路。一共发现5个促性腺细胞标记基因或其对应转录本与部分睾丸性状显著相关,包括NFASC(neurofascin)及其对应转录本XM_015298904.2、TESC(tescalcin)及其对应转录本XM_025155510.1、FSHB(folliclestimulatinghormonebeta)转录本ONT.20974.4、RLN3(relaxin 3)、NDUFB2(NADH:ubiquinone oxidoreductase subunit B2)转录本ONT.1176.4。1个催乳素细胞标记基因EGR1(early growth response 1),2个滤泡星状细胞标记基因TMSB4X(thymosin beta 4,X-linked)、C1H12ORF57(chromosome 1 open reading frame),1个促甲状腺细胞标记基因CHGB(chromogranin B)均与部分睾丸性状显著相关。2个高表达量基因TMSB4X、HSP90AA1(heat shock protein 90 alpha family class A member 1)与部分睾丸性状显著相关。FSHB、EGR1均为基因互作网络中节点基因。【结论】经初步功能分析,EGR1、TMSB4X、FSHB、RLN3为睾丸性状调控的关键基因。MAPK信号通路、神经活性配体-受体相互作用、GnRH信号通路为睾丸性状调控的关键基因通路。展开更多
文摘In order to</span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> study the meat quality characteristics of </span><span style="font-family:Verdana;">Ningdu</span><span style="font-family:Verdana;"> yellow rooster, the 7 indexes of 120 healthy </span><span style="font-family:Verdana;">Ningdu</span><span style="font-family:Verdana;"> yellow rooster</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> after </span><span style="font-family:Verdana;">slaughter</span><span style="font-family:Verdana;"> at the age of 16 weeks were determined, and correlation analysis, regression analysis </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> principal component analysis were carried out among the indexes.</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">The results showed that the variation coefficient of PH value was smaller than that of water drop rate and meat colorimetric, and the lactic acid produced by anaerobic glycolysis of glycogen in chicken meat was slower.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">Through correlation analysis, the 24-hour drip rate was strongly correlated with the 48-hour drip rate (coefficient ≥</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">0.7,</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">p</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">≤</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">0.05), and the other indicators were independent of each other.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">By means of</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> regression analysis, the regression equation (fitting degree R</span><sup><span style="font-family:Verdana;">2</span></sup></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">=</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">0.984) of </span><span style="font-family:Verdana;">24</span><span style="font-family:Verdana;">- and 48-hour drip rate was established.</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">Principal component analysis synthesized 7 indicators into 3 principal components, with a cumulative contribution rate of 70.13%, indicating that the meat quality of Ningdu Yellow chicken rooster is mainly selected by water drop rate, PH value </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> meat color test.
基金supported by China Geological Survey (DD20190083, DD20221662)National Natural Science Foundation of China (41904044, 41974064, 42174076, 41874069)Youth Innovation Promotion Association CAS (2019330).
文摘The Ningdu basin,located in southern Jiangxi province of southwest China,is one of the Mesozoic basin groups which has exploration prospects for geothermal energy.A study on the detailed velocity structure of the Ningdu basin can provide important information for geothermal resource exploration.In this study,we deployed a dense seismic array in the Ningdu basin to investigate the 3D velocity structure and discuss implications for geothermal exploration and geological evolution.Based on the dense seismic array including 35 short-period(5 s-100 Hz)seismometers with an average interstation distance of~5 km,Rayleigh surface wave dispersion curves were extracted from the continuous ambient noise data for surface wave tomographic inversion.Group velocity tomography was conducted and the 3D S-wave velocity structure was inverted by the neighborhood algorithm.The results revealed obvious low-velocity anomalies in the center of the basin,consistent with the low-velocity Cretaceous sedimentary rocks.The basement and basin-controlling fault can also be depicted by the S-wave velocity anomalies.The obvious seismic interface is about 2 km depth in the basin center and decreases to 700 m depth near the basin boundary,suggesting spatial thickness variations of the Cretaceous sediment.The fault features of the S-wave velocity profile coincide with the geological cognition of the western boundary basincontrolling fault,which may provide possible upwelling channels for geothermal fluid.This study suggests that seismic tomography with a dense array is an effective method and can play an important role in the detailed investigations of sedimentary basins.
文摘试验旨在探讨成年宁都黄鸡睾丸的蛋白质表达谱,筛选大小睾丸个体差异表达蛋白和功能通路,为繁殖性能选育提供基础数据。以6只22周龄宁都黄鸡的睾丸组织为研究对象,分为大睾丸组(H-TES)和小睾丸组(L-TES),采用串联质量标签(tandem mass tags, TMT)技术对2组个体睾丸蛋白质进行差异表达、功能富集和基因互作分析,初步筛选候选蛋白质,分析这些蛋白质在6个个体的表达量与6个睾丸性状指标(左侧睾丸重、右侧睾丸重、总睾丸重、左侧睾丸指数、右侧睾丸指数、总睾丸指数)之间的相关性,分析候选蛋白质的功能。结果表明,宁都黄鸡睾丸中共鉴定到4 683个蛋白质,筛选到144个差异表达蛋白质。GO分析结果表明,差异蛋白质主要参与精子结构组成,具有尿蛋白-谷氨酸连接酶活性、胸腺嘧啶结合、脱氢抗坏血酸跨膜转运体活性等功能。差异蛋白质显著富集的KEGG通路为精氨酸和脯氨酸代谢、泛酸和辅酶A的生物合成、β-丙氨酸代谢等。蛋白质互作网络中有34个差异蛋白质,初步筛选出30个候选蛋白质。结合相关性分析和文献查阅,筛选出16个关键候选蛋白质,即SPAG6、TPPP2、ENKUR、ODF2、SAXO1、TCP11、DNALI1、CCDC63、TSSK3、TEKT2、TEKT3、ALDH2、DNAH5、CCDC40、CCDC39、DNAH10。结合关键候选蛋白质的功能,初步确定精氨酸和脯氨酸代谢、泛酸和辅酶A生物合成、β-丙氨酸代谢、紧密连接、间隙连接是睾丸性状调控关键KEGG通路。研究结果为地方鸡种睾丸质量性状选育提供了基础依据。
文摘为研究不同养殖方式下宁都黄鸡肌肉关键挥发性风味物质,将试验鸡随机分为笼养组和平养组,饲喂同一日粮。试验鸡达上市日龄时对鸡肉进行感官品尝评价和挥发性风味物质检测,并采用正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)方法筛选与不同养殖方式相关的差异性风味物质。结果表明:平养组和笼养组共有的挥发性风味物质27种,主要为酚类、醇类和烃类。挥发性风味物质中,己醛、1-辛烯-3-醇、E-2-壬烯醛、正己醇、壬醛、2,3-戊二酮、癸醛、2,3-辛二酮、E-2-辛烯醛为具有显著性差异的挥发性风味物质。综上,这一研究可为地方鸡肉品质基于风味物质的评价提供科学依据。
文摘【背景】睾丸是雄性动物重要生殖器官,具有产生精子和分泌雄激素的功能,睾丸质量与繁殖性能有直接联系。睾丸性状受下丘脑-垂体-性腺轴多基因共同调控,其分子机理尚不清楚。宁都黄鸡睾丸性状变异系数大,繁殖性能一致性差。【目的】通过对不同睾丸性状个体垂体组织进行全长转录组测序,挖掘候选基因以及关键信号通路,探究影响睾丸性状的遗传基础,以期为发展地方鸡种睾丸性状的选种选育提供依据。【方法】以70只22周龄宁都黄公鸡为研究对象,分为大睾丸组和小睾丸组,从两组中分别选取3个个体,构建垂体组织的全长转录本表达谱,筛选高表达量及两组个体差异表达基因或转录本,对差异基因或转录本进行功能富集分析。随机选取6个基因,进行基因表达水平验证。参考已知垂体细胞的标记基因,筛选与8种细胞标记基因对应的差异表达基因或转录本,并分析这些差异基因或转录本以及高表达量基因或转录本与睾丸性状相关性。【结果】在6个宁都黄鸡垂体组织中,检测到21834个基因,29355个全长转录本。其中,332个基因及229个转录本在两组个体中差异表达。KEGG通路分析结果显示,这些差异基因或转录本主要富集于Apelin信号、甘油磷脂代谢等通路。一共发现5个促性腺细胞标记基因或其对应转录本与部分睾丸性状显著相关,包括NFASC(neurofascin)及其对应转录本XM_015298904.2、TESC(tescalcin)及其对应转录本XM_025155510.1、FSHB(folliclestimulatinghormonebeta)转录本ONT.20974.4、RLN3(relaxin 3)、NDUFB2(NADH:ubiquinone oxidoreductase subunit B2)转录本ONT.1176.4。1个催乳素细胞标记基因EGR1(early growth response 1),2个滤泡星状细胞标记基因TMSB4X(thymosin beta 4,X-linked)、C1H12ORF57(chromosome 1 open reading frame),1个促甲状腺细胞标记基因CHGB(chromogranin B)均与部分睾丸性状显著相关。2个高表达量基因TMSB4X、HSP90AA1(heat shock protein 90 alpha family class A member 1)与部分睾丸性状显著相关。FSHB、EGR1均为基因互作网络中节点基因。【结论】经初步功能分析,EGR1、TMSB4X、FSHB、RLN3为睾丸性状调控的关键基因。MAPK信号通路、神经活性配体-受体相互作用、GnRH信号通路为睾丸性状调控的关键基因通路。
