Nitrogen(N)is crucial for maize(Zea mays L.)growth,development and yield.Dysfunction of the maize NIN-like protein 5(ZmNLP5)reduces N assimilation,but the precise mechanism by which ZmNLP5 modulates N metabolism and i...Nitrogen(N)is crucial for maize(Zea mays L.)growth,development and yield.Dysfunction of the maize NIN-like protein 5(ZmNLP5)reduces N assimilation,but the precise mechanism by which ZmNLP5 modulates N metabolism and its contribution to agricultural applications are not well understood.In this study,our transcriptomic profiling and chromatin immunoprecipitation followed by sequencing(ChIP-seq)analyses reveal 581 ZmNLP5-modulated target genes.We confirmed that ZmNLP5 physically interacted and transactivated contributors to N assimilation,including ZmNRT1.1D,ZmNIR1.1,ZmNR2.1,ZmGS4,ZmAS1,and ZmLBD6.Overexpression of ZmNLP5 upregulated N metabolism genes,and enhanced the enzymatic activities of nitrate reductase and glutamine synthase.Furthermore,overexpressing ZmNLP5 increased grain yield under both normal-N and low-N conditions.Introgression of the overexpressed ZmNLP5 allele into Zhengdan 958 resulted in comparable yield increases in field trials.Our study unveiled that ZmNLP5 is a potential genetic target for increasing nitrogen use efficiency and grain yield in maize.展开更多
Background:Nitrogen(N)is a required macronutrient for cotton growth and productivity.Excessive N fertilizers are applied in agriculture for crop yield maximization,which also generates environmental pollution.Improvin...Background:Nitrogen(N)is a required macronutrient for cotton growth and productivity.Excessive N fertilizers are applied in agriculture for crop yield maximization,which also generates environmental pollution.Improving crop N use efficiency(NUE)is the most economical and desirable way of reducing fertilizer application and environmental pollution.NUE has been an important issue in cotton.So far there is no report on cotton NUE improvement via transgenic approach.Nin-like proteins(NLP)are transcription factors regulating NUE.We previously demonstrated that At NLP7 improved NUE and biomass when overexpressed in Arabidopsis.However,it is not known whether At NLP7 can be used to improve NUE in crops.Results:To test the feasibility,we expressed At NLP7 in cotton and evaluated NUE and yield of the transgenic cotton in the field.Transgenic cotton showed improved NUE and yield under both low and high N conditions.In addition,plant biomass,amount of absorbed N,N contents,activities of N-assimilating enzymes,and the expression of N-related marker genes were significantly increased in transgenic cotton compared with the wild type control,suggesting that At NLP7 enhances NUE in cotton.Conclusion:Together,our results demonstrate that At NLP7 is a promising candidate to improve NUE and yield in cotton.展开更多
Nitrogen fertilization has increased wheat yields since the Green Revolution,but these gains have plateaued.Excessive nitrogen application reduces nitrogen use efficiency by promoting nonproductive tillers,and balanci...Nitrogen fertilization has increased wheat yields since the Green Revolution,but these gains have plateaued.Excessive nitrogen application reduces nitrogen use efficiency by promoting nonproductive tillers,and balancing nitrogen uptake with tillering in wheat remains a challenge.Here,we demonstrate that TaNLP3 is a master regulator of nitrate signaling that,together with the SWl/SNF complex,regulates chromatin accessibility to fine-tune nitrate uptake and tiller formation through a temporal transcriptional cascade.In short-term nitrate signaling,TaNLP3 activates the expression of primary nitrate response genes,including TaNRT2.1,to promote nitrate uptake.In long-term nitrate signaling,TaLBD38 is induced by TaNLP3 and represses TaNRT2.1,limiting nitrate uptake and promoting tillering by inhibiting TaCKX4/5,negative modulators of tillering.Furthermore,we identified elite haplotypes of TaNLP3-3B,TaLBD38-4A,andTaNRT2.1-6B4 that enable higher yields under equivalent nitrogen supply.Taken together,our findings reveal the dynamic coordination between nitrate uptake and tillering under fluctuating nitrogen conditions,offering valuable resources for breeding wheat varieties with improved nitrogen use efficiency and productivity.展开更多
基金supported by grants from the National Natural Science Foundation of China(32001564,32372031,32272133,and 32372101)the“JBGS”Project of Seed Industry Revitalization in Jiangsu Province(JBGS[2021]012).
文摘Nitrogen(N)is crucial for maize(Zea mays L.)growth,development and yield.Dysfunction of the maize NIN-like protein 5(ZmNLP5)reduces N assimilation,but the precise mechanism by which ZmNLP5 modulates N metabolism and its contribution to agricultural applications are not well understood.In this study,our transcriptomic profiling and chromatin immunoprecipitation followed by sequencing(ChIP-seq)analyses reveal 581 ZmNLP5-modulated target genes.We confirmed that ZmNLP5 physically interacted and transactivated contributors to N assimilation,including ZmNRT1.1D,ZmNIR1.1,ZmNR2.1,ZmGS4,ZmAS1,and ZmLBD6.Overexpression of ZmNLP5 upregulated N metabolism genes,and enhanced the enzymatic activities of nitrate reductase and glutamine synthase.Furthermore,overexpressing ZmNLP5 increased grain yield under both normal-N and low-N conditions.Introgression of the overexpressed ZmNLP5 allele into Zhengdan 958 resulted in comparable yield increases in field trials.Our study unveiled that ZmNLP5 is a potential genetic target for increasing nitrogen use efficiency and grain yield in maize.
基金supported by grants from Ministry of Science and Technology of China(Grant No.2016ZX08005004-003).
文摘Background:Nitrogen(N)is a required macronutrient for cotton growth and productivity.Excessive N fertilizers are applied in agriculture for crop yield maximization,which also generates environmental pollution.Improving crop N use efficiency(NUE)is the most economical and desirable way of reducing fertilizer application and environmental pollution.NUE has been an important issue in cotton.So far there is no report on cotton NUE improvement via transgenic approach.Nin-like proteins(NLP)are transcription factors regulating NUE.We previously demonstrated that At NLP7 improved NUE and biomass when overexpressed in Arabidopsis.However,it is not known whether At NLP7 can be used to improve NUE in crops.Results:To test the feasibility,we expressed At NLP7 in cotton and evaluated NUE and yield of the transgenic cotton in the field.Transgenic cotton showed improved NUE and yield under both low and high N conditions.In addition,plant biomass,amount of absorbed N,N contents,activities of N-assimilating enzymes,and the expression of N-related marker genes were significantly increased in transgenic cotton compared with the wild type control,suggesting that At NLP7 enhances NUE in cotton.Conclusion:Together,our results demonstrate that At NLP7 is a promising candidate to improve NUE and yield in cotton.
基金supported by the National Natural Science Foundation of China(nos.32572386 and U22A6009)the Hebei Key Science and Technology Support Program(242Q9911Z)+3 种基金the Guiding Special Fund for Central Universities to Build World-Class Universities(Disciplines)and Promote Characteristic Development(2025AC030)the National Key Research and Development Program of China(no.2023YFF1000601)the Chinese Universities Scientific Fund(no.2025TC135)the Agricultural Science and Technology Major Project.
文摘Nitrogen fertilization has increased wheat yields since the Green Revolution,but these gains have plateaued.Excessive nitrogen application reduces nitrogen use efficiency by promoting nonproductive tillers,and balancing nitrogen uptake with tillering in wheat remains a challenge.Here,we demonstrate that TaNLP3 is a master regulator of nitrate signaling that,together with the SWl/SNF complex,regulates chromatin accessibility to fine-tune nitrate uptake and tiller formation through a temporal transcriptional cascade.In short-term nitrate signaling,TaNLP3 activates the expression of primary nitrate response genes,including TaNRT2.1,to promote nitrate uptake.In long-term nitrate signaling,TaLBD38 is induced by TaNLP3 and represses TaNRT2.1,limiting nitrate uptake and promoting tillering by inhibiting TaCKX4/5,negative modulators of tillering.Furthermore,we identified elite haplotypes of TaNLP3-3B,TaLBD38-4A,andTaNRT2.1-6B4 that enable higher yields under equivalent nitrogen supply.Taken together,our findings reveal the dynamic coordination between nitrate uptake and tillering under fluctuating nitrogen conditions,offering valuable resources for breeding wheat varieties with improved nitrogen use efficiency and productivity.
