Organic luminophores with superior solid-state luminescence are urgently required in various fields,such as lighting,display,sensing,and solar energy conversion.However,to achieve their highly efficient luminescence s...Organic luminophores with superior solid-state luminescence are urgently required in various fields,such as lighting,display,sensing,and solar energy conversion.However,to achieve their highly efficient luminescence still remains a challenge.Herein,a newly designed Nile red derivative,Nile-DPA-VB,is successfully obtained to exhibit aggregation-induced emission characteristics with the photoluminescent quantum yield(PLQY)of 11.45%.Such PLQY could be further promoted to 53.45%when Nile-DPA-VB is polymerized undergoing precipitation polymerization process,where the confined aggregation microenvironment severely restricts the intramolecular motions of Nile-DPA-VB.Remarkably,Nile-DPA-VB is ultrasensitive to the polarity and steric effect,enabling the real-time monitoring of aggregation microenvironment evolution for precipitation polymerization.The microphase separation and dynamic hardening for the nucleation and growth processes are visually demonstrated,which contribute dominantly to the high-efficiency luminescence.Finally,by doping the as-prepared fluorescent polymeric particles into polymethyl methacrylate,functional films with high luminescence and high haze are achieved to show the potential in lighting.These findings clearly demonstrate the significant role of polymerization in constructing high-efficiency solid-state luminescent materials for practice.展开更多
The escalating threats of antimicrobial resistance and monkeypox virus infections pose a significant challenge to public health,necessitating innovative therapeutic approaches.Developing materials with balanced photod...The escalating threats of antimicrobial resistance and monkeypox virus infections pose a significant challenge to public health,necessitating innovative therapeutic approaches.Developing materials with balanced photodynamic and photothermal effects for the elimination of broad-spectrum drug-resistant bacteria and inactivation of the monkeypox virus remains a formidable task.Herein,we prepared a series of Nile Red derivatives by a donor rotation and charge transfer enhancement strategy,identifying 5-(dicyanomethylene)-9-[4-(bis(4-methoxyphenyl)amino)phenyl]-7a,12a-dihydro-5H-benzo[a]phenoxazine(TPAOMCN)-featuring alkoxy-triphenylamine and malononitrile,as the optimal candidate.TPAOMCN demonstrated extended near-infrared absorption,enhanced intersystem crossing(ISC)efficiency,and intense molecular motions,enabling dual-modal phototherapy.Electrospun TPAOMCN nanofibers(NFs)with submicron-scale diameter achieved>50℃temperature elevation and excellent reactive oxygen species(ROS)generation under irradiation.In methicillin-resistant S.aureus(MRSA)-induced wound infection and vaccinia virus-mediated tail-scarred models,TPAOMCN NFs effectively eliminated MRSA colonies and reduced viral load through physical disruption of pathogen membranes,thermal denaturation of viral capsids,and ROS-mediated oxidation of biomolecules,while suppressing inflammation and accelerating angiogenesis-mediated tissue repair.This study not only established a molecular engineering strategy for Nile Red to achieve prime PDT-PTT performance but also provided a paradigm for advancing dual-functional phototherapeutic platforms against emerging antimicrobial threats and monkeypox virus infections.展开更多
A new sensitive fluorometric assay method for acetylcholinesterase （ACHE） and its inhibitor was developed us- ing a fluorescent dye, nile red （NR）. Due to the fluorescence resonance energy transfer between the NR ...A new sensitive fluorometric assay method for acetylcholinesterase （ACHE） and its inhibitor was developed us- ing a fluorescent dye, nile red （NR）. Due to the fluorescence resonance energy transfer between the NR and the gold nanoparticle （AuNPs）, the fluorescence was quenched. AChE can break down acetylthiocholine to produce a thiol-bearing compound, thiocholine. In the presence of thiocholine, the nile red is replaced from the AuNPs sur- faces and simultaneously transformed to a derivative of nile red. The fluorescence intensity of the derivative is much stronger than that of the native nile red with the same concentration and its maximum emission wavelength has a blue shift so that the sensor achieves a good signal-to-background ratio. In addition, when organophosphate pesticide （OPs） exists, the activity of AChE can be inhibited, the generation of thiocholine will be prevented and no fluorescence enhancement occurs. The results show that the method is sensitive to AChE and paraoxon with the de- tection limits of 0.2 mU/mL and 0.05 ng/mL, respectively.展开更多
A range-extended acidity detector based on Nile red was designed and analyzed in this work. In light of the good lasing property and solvatochromism characteristic of Nile red/ethanol solution, we have obtained laser ...A range-extended acidity detector based on Nile red was designed and analyzed in this work. In light of the good lasing property and solvatochromism characteristic of Nile red/ethanol solution, we have obtained laser spectra of sulfuric acid in different concentrations doped in this substrate. Moreover, to expand the acidity detection range, we proposed a tandem cuvette system containing rhodamine 6G/ethanol and Nile red/ethanol. Consequently, the detection range could be enlarged from 26 nm to 40 nm, by changing not only the wavelength peak but also by the intensity ratio of dual-wavelength laser output. In addition, by changing the detection and substrate materials, the whole detection range could be expanded, and therefore a wide range of applications in polarity and acidity detection could be implemented via this method.展开更多
Microplastics(MPs)have been widely detected in marine environments,yet the pollution in terrestrial fresh waters in economically underdeveloped and remote areas is poorly understood.We investigated the abundance and d...Microplastics(MPs)have been widely detected in marine environments,yet the pollution in terrestrial fresh waters in economically underdeveloped and remote areas is poorly understood.We investigated the abundance and distribution of MPs in inland waters(including open waters in river,lake,reservoir,stream,and tap water and bottled water)in Guizhou,SW China,detected by Nile Red staining.Results show that MPs were detected in all samples,with the mean abundance of 3.5±1.9 items/mL in 34river water samples,3.9±1.9 items/mL in 14 lake and reservoir water samples,3.4±2.4 items/mL in 87 stream water samples,3.8±3.8 items/mL in 164 tap water samples,and 1.3±3.2 items/mL in 12 bottled water samples from 4 different brands.The MP abundance in open field waters(3.6±3.0 items/mL)was significantly higher than that in bottled water(1.3±3.2 items/mL)(P<0.05).The microplastics with particle sizes of 10-20μm accounted for more than 50%of all samples.The composition ratio of smalland medium-sized microplastics in open waters was higher than in tap and bottled water(P<0.05).The MPs abundance tended to increase with the decrease in particle size.Therefore,microplastic pollution varied in different degrees in open waters in economically underdeveloped and remote areas of the southwestern China,which is consistent with microplastic pollution in other similar water environments.This study provided a reference for understanding the microplastic content and pollution control in the inland waters in western and remote areas of China.展开更多
基金supported by the National Natural Science Foundation of China(52373171,52173154,52221006,22375014)the Beijing Natural Science Foundation Grant(JQ23006),and the Fundamental Research Funds for the Central Universities(ZY2404).
文摘Organic luminophores with superior solid-state luminescence are urgently required in various fields,such as lighting,display,sensing,and solar energy conversion.However,to achieve their highly efficient luminescence still remains a challenge.Herein,a newly designed Nile red derivative,Nile-DPA-VB,is successfully obtained to exhibit aggregation-induced emission characteristics with the photoluminescent quantum yield(PLQY)of 11.45%.Such PLQY could be further promoted to 53.45%when Nile-DPA-VB is polymerized undergoing precipitation polymerization process,where the confined aggregation microenvironment severely restricts the intramolecular motions of Nile-DPA-VB.Remarkably,Nile-DPA-VB is ultrasensitive to the polarity and steric effect,enabling the real-time monitoring of aggregation microenvironment evolution for precipitation polymerization.The microphase separation and dynamic hardening for the nucleation and growth processes are visually demonstrated,which contribute dominantly to the high-efficiency luminescence.Finally,by doping the as-prepared fluorescent polymeric particles into polymethyl methacrylate,functional films with high luminescence and high haze are achieved to show the potential in lighting.These findings clearly demonstrate the significant role of polymerization in constructing high-efficiency solid-state luminescent materials for practice.
基金supported by National Natural Science Foundation of China(82271948)China Postdoctoral Science Foundation(2024M760593)+5 种基金2023 Postdoctoral Workstation Project(2023BSGZ011)Lifting Project of Guangdong Second Provincial General Hospital(TJGC-2023001)Guangdong Medical Research Fund of 2025(B2025545)Experts and Youths Project of Guangdong Second Provincial General Hospital(2024B002)Fujian Province Science and Technology Plan(2024D022)2024 Smart Imaging New Technology Innovation Fund(NMED2024CX-01-005).
文摘The escalating threats of antimicrobial resistance and monkeypox virus infections pose a significant challenge to public health,necessitating innovative therapeutic approaches.Developing materials with balanced photodynamic and photothermal effects for the elimination of broad-spectrum drug-resistant bacteria and inactivation of the monkeypox virus remains a formidable task.Herein,we prepared a series of Nile Red derivatives by a donor rotation and charge transfer enhancement strategy,identifying 5-(dicyanomethylene)-9-[4-(bis(4-methoxyphenyl)amino)phenyl]-7a,12a-dihydro-5H-benzo[a]phenoxazine(TPAOMCN)-featuring alkoxy-triphenylamine and malononitrile,as the optimal candidate.TPAOMCN demonstrated extended near-infrared absorption,enhanced intersystem crossing(ISC)efficiency,and intense molecular motions,enabling dual-modal phototherapy.Electrospun TPAOMCN nanofibers(NFs)with submicron-scale diameter achieved>50℃temperature elevation and excellent reactive oxygen species(ROS)generation under irradiation.In methicillin-resistant S.aureus(MRSA)-induced wound infection and vaccinia virus-mediated tail-scarred models,TPAOMCN NFs effectively eliminated MRSA colonies and reduced viral load through physical disruption of pathogen membranes,thermal denaturation of viral capsids,and ROS-mediated oxidation of biomolecules,while suppressing inflammation and accelerating angiogenesis-mediated tissue repair.This study not only established a molecular engineering strategy for Nile Red to achieve prime PDT-PTT performance but also provided a paradigm for advancing dual-functional phototherapeutic platforms against emerging antimicrobial threats and monkeypox virus infections.
文摘A new sensitive fluorometric assay method for acetylcholinesterase （ACHE） and its inhibitor was developed us- ing a fluorescent dye, nile red （NR）. Due to the fluorescence resonance energy transfer between the NR and the gold nanoparticle （AuNPs）, the fluorescence was quenched. AChE can break down acetylthiocholine to produce a thiol-bearing compound, thiocholine. In the presence of thiocholine, the nile red is replaced from the AuNPs sur- faces and simultaneously transformed to a derivative of nile red. The fluorescence intensity of the derivative is much stronger than that of the native nile red with the same concentration and its maximum emission wavelength has a blue shift so that the sensor achieves a good signal-to-background ratio. In addition, when organophosphate pesticide （OPs） exists, the activity of AChE can be inhibited, the generation of thiocholine will be prevented and no fluorescence enhancement occurs. The results show that the method is sensitive to AChE and paraoxon with the de- tection limits of 0.2 mU/mL and 0.05 ng/mL, respectively.
基金supported by the National Key Research and Development Program of China(No. 2016YFB0401901)Major Science and Technology Special Project in Anhui (No. 17030901001)+2 种基金National Natural Science Foundation of China (Nos. 21574120and 21774115)Fundamental Research Funds for the Central Universities (No. WK2060200025)Science and Technological Fund of Anhui Province for Outstanding Youth (No. 1608085J01)。
文摘A range-extended acidity detector based on Nile red was designed and analyzed in this work. In light of the good lasing property and solvatochromism characteristic of Nile red/ethanol solution, we have obtained laser spectra of sulfuric acid in different concentrations doped in this substrate. Moreover, to expand the acidity detection range, we proposed a tandem cuvette system containing rhodamine 6G/ethanol and Nile red/ethanol. Consequently, the detection range could be enlarged from 26 nm to 40 nm, by changing not only the wavelength peak but also by the intensity ratio of dual-wavelength laser output. In addition, by changing the detection and substrate materials, the whole detection range could be expanded, and therefore a wide range of applications in polarity and acidity detection could be implemented via this method.
基金Supported by the National Key Research and Development Program of China(Nos.2018YFC1004300,2018YFC1004302)the Science&Technology Program of Guizhou Province(Nos.QKHHBZ[2020]3002,QKHPTRC-GCC[2022]039-1,QKH-J[2022]YB614)+1 种基金the Science&Technology Program of Zunyi City(No.ZHZ[2021]294)the National Innovation Program for College Students in China(No.201910661012)。
文摘Microplastics(MPs)have been widely detected in marine environments,yet the pollution in terrestrial fresh waters in economically underdeveloped and remote areas is poorly understood.We investigated the abundance and distribution of MPs in inland waters(including open waters in river,lake,reservoir,stream,and tap water and bottled water)in Guizhou,SW China,detected by Nile Red staining.Results show that MPs were detected in all samples,with the mean abundance of 3.5±1.9 items/mL in 34river water samples,3.9±1.9 items/mL in 14 lake and reservoir water samples,3.4±2.4 items/mL in 87 stream water samples,3.8±3.8 items/mL in 164 tap water samples,and 1.3±3.2 items/mL in 12 bottled water samples from 4 different brands.The MP abundance in open field waters(3.6±3.0 items/mL)was significantly higher than that in bottled water(1.3±3.2 items/mL)(P<0.05).The microplastics with particle sizes of 10-20μm accounted for more than 50%of all samples.The composition ratio of smalland medium-sized microplastics in open waters was higher than in tap and bottled water(P<0.05).The MPs abundance tended to increase with the decrease in particle size.Therefore,microplastic pollution varied in different degrees in open waters in economically underdeveloped and remote areas of the southwestern China,which is consistent with microplastic pollution in other similar water environments.This study provided a reference for understanding the microplastic content and pollution control in the inland waters in western and remote areas of China.
