Mei Niang （1920-2013）, the pen name of Sun Jiarui, is a female fiction writer, translator, and editor of Funii zazhi （Ladies＇ journal）. In the semi-colonial Northeast China, Mei Niang＇s exploration of melancholi...Mei Niang （1920-2013）, the pen name of Sun Jiarui, is a female fiction writer, translator, and editor of Funii zazhi （Ladies＇ journal）. In the semi-colonial Northeast China, Mei Niang＇s exploration of melancholic narratives shore up manifold levels of socio-historical discourses that are constructive of women＇s subjectivity. Melancholic narrative functions as an inverted mirror of both the author＇s cultural displacement from her diasporic experience, and her portrayal of colonial domination of local elites by the Japanese in Northeast China. Also, the author＇s depiction of feminine melan- cholia revokes the modernist ideology of love and its constitutive male-centered discourses, dismantles the social disenfranchisement of women by feudal authority, social prejudices, and the eroding urban materialism. Stylistically, Mei Niang＇s second-person texts reinforce the feminine authority in enunciation, and by articulating women＇s emotions and afflictions, transmogrify the melancholic narrative into a mode of self-narration and self-empowerment.展开更多
采用七叶苷分离培养基初筛、4-硝基苯-β-D-吡喃葡萄糖苷(p-NPGal)显色法复筛的方法从广东客家娘酒发酵过程中的酒糟中筛选产β-葡萄糖苷酶能力较强的酵母菌,通过26S r DNA D1/D2区基因序列分析对其进行鉴定,并对其酶学性质进行分析。...采用七叶苷分离培养基初筛、4-硝基苯-β-D-吡喃葡萄糖苷(p-NPGal)显色法复筛的方法从广东客家娘酒发酵过程中的酒糟中筛选产β-葡萄糖苷酶能力较强的酵母菌,通过26S r DNA D1/D2区基因序列分析对其进行鉴定,并对其酶学性质进行分析。结果表明,获得1株产β-葡萄糖苷酶能力较强的假丝酵母Candida apicola kj312。该菌株所产的β-葡萄糖苷酶具有较宽的底物特异性,最适底物为对硝基苯基-β-D-吡喃葡萄糖苷;最适反应温度为60℃,在25~65℃范围内,相对酶活力>50%;最适p H值为4.5,在p H值为4.0~7.0酸性范围内,相对酶活力>80%;1 mmol/L的Ca2+、Mn2+、Zn2+和Fe2+及100 mmol/L的Ca2+、Zn2+和Fe2+能显著提高酶活力。展开更多
文摘Mei Niang （1920-2013）, the pen name of Sun Jiarui, is a female fiction writer, translator, and editor of Funii zazhi （Ladies＇ journal）. In the semi-colonial Northeast China, Mei Niang＇s exploration of melancholic narratives shore up manifold levels of socio-historical discourses that are constructive of women＇s subjectivity. Melancholic narrative functions as an inverted mirror of both the author＇s cultural displacement from her diasporic experience, and her portrayal of colonial domination of local elites by the Japanese in Northeast China. Also, the author＇s depiction of feminine melan- cholia revokes the modernist ideology of love and its constitutive male-centered discourses, dismantles the social disenfranchisement of women by feudal authority, social prejudices, and the eroding urban materialism. Stylistically, Mei Niang＇s second-person texts reinforce the feminine authority in enunciation, and by articulating women＇s emotions and afflictions, transmogrify the melancholic narrative into a mode of self-narration and self-empowerment.
文摘采用七叶苷分离培养基初筛、4-硝基苯-β-D-吡喃葡萄糖苷(p-NPGal)显色法复筛的方法从广东客家娘酒发酵过程中的酒糟中筛选产β-葡萄糖苷酶能力较强的酵母菌,通过26S r DNA D1/D2区基因序列分析对其进行鉴定,并对其酶学性质进行分析。结果表明,获得1株产β-葡萄糖苷酶能力较强的假丝酵母Candida apicola kj312。该菌株所产的β-葡萄糖苷酶具有较宽的底物特异性,最适底物为对硝基苯基-β-D-吡喃葡萄糖苷;最适反应温度为60℃,在25~65℃范围内,相对酶活力>50%;最适p H值为4.5,在p H值为4.0~7.0酸性范围内,相对酶活力>80%;1 mmol/L的Ca2+、Mn2+、Zn2+和Fe2+及100 mmol/L的Ca2+、Zn2+和Fe2+能显著提高酶活力。
