We established a stroke-prone renovascular hypertensive rat model by bilateral constriction of the renal artery with sliver loop clips. After ten weeks, middle cerebral artery occlusion was induced for 2 hours. The ra...We established a stroke-prone renovascular hypertensive rat model by bilateral constriction of the renal artery with sliver loop clips. After ten weeks, middle cerebral artery occlusion was induced for 2 hours. The rats then received electro-acupuncture at Baihui （DU 20） and Dazhui （DU 14） after onset of ischemia for 30 days. In situ hybridization study showed that electroacupuncture significantly reduced the number of neurocan mRNA-positive cells in the ischemic penumbra and hippocampal tissues of rats. Electron microscopy results demonstrated that the structure of neurons and blood vessels in the ischemic tissues were restored with electroacupuncture. Overall, these data suggest that electroacupuncture may protect neurons against ischemic reperfusion injury in stroke-prone renovascular hypertensive rats, which may be regulated by downregulation of expression of nerve inhibitory factor neurocan mRNA.展开更多
BACKGROUND： Experimental data indicate that human growth-associated protein 43 mRNA expression coincides with axonal growth during nerve ganglion development; while neurocan, secreted from astrocytes, can inhibit spr...BACKGROUND： Experimental data indicate that human growth-associated protein 43 mRNA expression coincides with axonal growth during nerve ganglion development; while neurocan, secreted from astrocytes, can inhibit sprouting and elongation of the axonal growth cone. OBJECTIVE： To verify regulatory effects of cyclovirobuxine D （CVB-D） extracted from Chinese box branchlet on human growth-associated protein 43 （GAP-43）, and neurocan expression in brain tissue of stroke-prone renovascular hypertensive （RHRSP） rats, at different time points after cerebral ischemia/reperfusion. DESIGN： Randomized grouping design and controlled animal study. SETTING： This study was performed at the Center of Guangdong Hospital of Traditional Chinese Medicine （a national key laboratory） from March 2003 to September 2006. MATERIALS： 100 healthy male Sprague-Dawley rats, aged 2 3 months and weighing 90-120 g, were selected for this study. CVB-D was provided by Nanjing Xiaoying Pharmaceutical Factory （Batch number： 307701）. METHODS： The initial tip of renal arteries was clamped bilaterally for 10 weeks to establish the RHRSP model. 100 RHRSP rats were randomly divided into 4 groups： naive group （n = 10）, sham surgery group （n = 10）, CVB-D group （n = 40）, and lesion group （n = 40）. Rats in the naive group did not undergo any treatment, and cervical vessels of rats in the sham surgery group were exposed, but not blocked. The right middle cerebral artery of rats in the CVB-D group and lesion group were occluded to establish cerebral ischemia. Rats in the CVB-D group were intraperitoneally injected with CVB-D （6.48 mg/kg） every day and with saline （1.5 mL/injection） twice a day. Rats in the lesion group were intraperitoneally injected with saline （2 mL/injection）. MAIN OUTCOME MEASURES： Immunohistochemistry was applied to detect GAP-43 and neurocan expression in the ischemic penumbra region of CVB-D and lesion brains at 2 hours post-cerebral ischemia and at 1, 7, 14, and 30 days post-perfusion （n = 10 at each time point）. Similarly, GAP-43 and neurocan expression was detected in the right hemisphere of naive and sham-operated animals. The results were expressed as positive cells. RESULTS： A total of 100 rats were included in the final analysis. The number of GAP-43 positive cells increased in the CVB-D group 1, 7, 14, and 30 days post-cerebral ischemia/perfusion compared to the lesion group, as indicated by a significant difference between the CVB-D and lesion group （P 〈 0.054）.01）. The number of neurocan-positive cells decreased in the CVB-D group on the first day compared to the model group; however, there was no significant difference between the two groups （P 〉 0.05）. On post-ischemia days 7, 14, and 30, the number of neurocan-positive cells in the CVB-D group was significantly less than in the lesion group （P 〈 0.05）. Both, GAP-43 and neurocan expression was not detectable in brains of naive and sham-operated animals. CONCLUSION： CVB-D treatment up-regulated GAP-43 expression and down-regulated neurocan expression in the ischemic region of RHRSP rats.展开更多
目的:研究对羟基苯甲醛调控脑缺血后反应性星形胶质细胞(RAs)促进神经祖细胞(NPCs)的迁移作用及机制。方法:新生24 h SD大鼠分离并培养脑皮质星形胶质细胞(Ast)及NPCs。采用氧糖剥夺/恢复(OGD/R)复制体外脑缺血模型,促进Ast活化为RAs并...目的:研究对羟基苯甲醛调控脑缺血后反应性星形胶质细胞(RAs)促进神经祖细胞(NPCs)的迁移作用及机制。方法:新生24 h SD大鼠分离并培养脑皮质星形胶质细胞(Ast)及NPCs。采用氧糖剥夺/恢复(OGD/R)复制体外脑缺血模型,促进Ast活化为RAs并与NPCs用Transwell共培养,结晶紫染色观察NPCs的迁移情况;采用Western blot法检测NPCs的凋亡因子BCL-2及BAX的表达及RAs中促进NPCs增殖及分化的促红细胞生成素(EPO)、抑制NPCs迁移的神经黏蛋白(Neurocan)、促进NPCs迁移的多唾液酸神经细胞黏附分子(PSA-NCAM);采用ELISA法检测RAs促进NPCs存活的脑源性神经营养因子(BDNF)。结果:与空白对照组相比,模型对照组NPCs迁移数量明显增加(P<0.05),BCL-2/BAX的比值明显降低(P<0.05),RAs的Neurocan蛋白表达显著上调(P<0.01),EPO、PSA-NCAM表达显著上调(P<0.01),BDNF的含量明显升高(P<0.05);用对羟基苯甲醛100μmol/L NPCs迁移数明显增加(P<0.05),BCL-2/BAX蛋白表达比值明显升高(P<0.05),RAs的Neurocan蛋白表达明显下调(P<0.05),EPO、PSA-NCAM蛋白表达明显上调(P<0.05),BDNF含量明显升高(P<0.05)。结论:对羟基苯甲醛能增加NPCs的迁移及存活,该作用可能与减少NPCs产生的凋亡因子、增加RAs产生的迁移及趋化因子、神经保护因子的表达有关。展开更多
基金Research Projects of Science and Technology Bureau of Foshan City, No. 04080131the Administration Bureau of Traditional Chinese Medicine of Guangdong Province, No. 1050006the Natural Science Foundation of Guangdong Province, No. 8152800007000001
文摘We established a stroke-prone renovascular hypertensive rat model by bilateral constriction of the renal artery with sliver loop clips. After ten weeks, middle cerebral artery occlusion was induced for 2 hours. The rats then received electro-acupuncture at Baihui （DU 20） and Dazhui （DU 14） after onset of ischemia for 30 days. In situ hybridization study showed that electroacupuncture significantly reduced the number of neurocan mRNA-positive cells in the ischemic penumbra and hippocampal tissues of rats. Electron microscopy results demonstrated that the structure of neurons and blood vessels in the ischemic tissues were restored with electroacupuncture. Overall, these data suggest that electroacupuncture may protect neurons against ischemic reperfusion injury in stroke-prone renovascular hypertensive rats, which may be regulated by downregulation of expression of nerve inhibitory factor neurocan mRNA.
基金the grants from Guangdong Province Administration of Traditional Chinese Medicine, No.103142
文摘BACKGROUND： Experimental data indicate that human growth-associated protein 43 mRNA expression coincides with axonal growth during nerve ganglion development; while neurocan, secreted from astrocytes, can inhibit sprouting and elongation of the axonal growth cone. OBJECTIVE： To verify regulatory effects of cyclovirobuxine D （CVB-D） extracted from Chinese box branchlet on human growth-associated protein 43 （GAP-43）, and neurocan expression in brain tissue of stroke-prone renovascular hypertensive （RHRSP） rats, at different time points after cerebral ischemia/reperfusion. DESIGN： Randomized grouping design and controlled animal study. SETTING： This study was performed at the Center of Guangdong Hospital of Traditional Chinese Medicine （a national key laboratory） from March 2003 to September 2006. MATERIALS： 100 healthy male Sprague-Dawley rats, aged 2 3 months and weighing 90-120 g, were selected for this study. CVB-D was provided by Nanjing Xiaoying Pharmaceutical Factory （Batch number： 307701）. METHODS： The initial tip of renal arteries was clamped bilaterally for 10 weeks to establish the RHRSP model. 100 RHRSP rats were randomly divided into 4 groups： naive group （n = 10）, sham surgery group （n = 10）, CVB-D group （n = 40）, and lesion group （n = 40）. Rats in the naive group did not undergo any treatment, and cervical vessels of rats in the sham surgery group were exposed, but not blocked. The right middle cerebral artery of rats in the CVB-D group and lesion group were occluded to establish cerebral ischemia. Rats in the CVB-D group were intraperitoneally injected with CVB-D （6.48 mg/kg） every day and with saline （1.5 mL/injection） twice a day. Rats in the lesion group were intraperitoneally injected with saline （2 mL/injection）. MAIN OUTCOME MEASURES： Immunohistochemistry was applied to detect GAP-43 and neurocan expression in the ischemic penumbra region of CVB-D and lesion brains at 2 hours post-cerebral ischemia and at 1, 7, 14, and 30 days post-perfusion （n = 10 at each time point）. Similarly, GAP-43 and neurocan expression was detected in the right hemisphere of naive and sham-operated animals. The results were expressed as positive cells. RESULTS： A total of 100 rats were included in the final analysis. The number of GAP-43 positive cells increased in the CVB-D group 1, 7, 14, and 30 days post-cerebral ischemia/perfusion compared to the lesion group, as indicated by a significant difference between the CVB-D and lesion group （P 〈 0.054）.01）. The number of neurocan-positive cells decreased in the CVB-D group on the first day compared to the model group; however, there was no significant difference between the two groups （P 〉 0.05）. On post-ischemia days 7, 14, and 30, the number of neurocan-positive cells in the CVB-D group was significantly less than in the lesion group （P 〈 0.05）. Both, GAP-43 and neurocan expression was not detectable in brains of naive and sham-operated animals. CONCLUSION： CVB-D treatment up-regulated GAP-43 expression and down-regulated neurocan expression in the ischemic region of RHRSP rats.
文摘目的:研究对羟基苯甲醛调控脑缺血后反应性星形胶质细胞(RAs)促进神经祖细胞(NPCs)的迁移作用及机制。方法:新生24 h SD大鼠分离并培养脑皮质星形胶质细胞(Ast)及NPCs。采用氧糖剥夺/恢复(OGD/R)复制体外脑缺血模型,促进Ast活化为RAs并与NPCs用Transwell共培养,结晶紫染色观察NPCs的迁移情况;采用Western blot法检测NPCs的凋亡因子BCL-2及BAX的表达及RAs中促进NPCs增殖及分化的促红细胞生成素(EPO)、抑制NPCs迁移的神经黏蛋白(Neurocan)、促进NPCs迁移的多唾液酸神经细胞黏附分子(PSA-NCAM);采用ELISA法检测RAs促进NPCs存活的脑源性神经营养因子(BDNF)。结果:与空白对照组相比,模型对照组NPCs迁移数量明显增加(P<0.05),BCL-2/BAX的比值明显降低(P<0.05),RAs的Neurocan蛋白表达显著上调(P<0.01),EPO、PSA-NCAM表达显著上调(P<0.01),BDNF的含量明显升高(P<0.05);用对羟基苯甲醛100μmol/L NPCs迁移数明显增加(P<0.05),BCL-2/BAX蛋白表达比值明显升高(P<0.05),RAs的Neurocan蛋白表达明显下调(P<0.05),EPO、PSA-NCAM蛋白表达明显上调(P<0.05),BDNF含量明显升高(P<0.05)。结论:对羟基苯甲醛能增加NPCs的迁移及存活,该作用可能与减少NPCs产生的凋亡因子、增加RAs产生的迁移及趋化因子、神经保护因子的表达有关。
