Human Activity Recognition(HAR)in drone-captured videos has become popular because of the interest in various fields such as video surveillance,sports analysis,and human-robot interaction.However,recognizing actions f...Human Activity Recognition(HAR)in drone-captured videos has become popular because of the interest in various fields such as video surveillance,sports analysis,and human-robot interaction.However,recognizing actions from such videos poses the following challenges:variations of human motion,the complexity of backdrops,motion blurs,occlusions,and restricted camera angles.This research presents a human activity recognition system to address these challenges by working with drones’red-green-blue(RGB)videos.The first step in the proposed system involves partitioning videos into frames and then using bilateral filtering to improve the quality of object foregrounds while reducing background interference before converting from RGB to grayscale images.The YOLO(You Only Look Once)algorithm detects and extracts humans from each frame,obtaining their skeletons for further processing.The joint angles,displacement and velocity,histogram of oriented gradients(HOG),3D points,and geodesic Distance are included.These features are optimized using Quadratic Discriminant Analysis(QDA)and utilized in a Neuro-Fuzzy Classifier(NFC)for activity classification.Real-world evaluations on the Drone-Action,Unmanned Aerial Vehicle(UAV)-Gesture,and Okutama-Action datasets substantiate the proposed system’s superiority in accuracy rates over existing methods.In particular,the system obtains recognition rates of 93%for drone action,97%for UAV gestures,and 81%for Okutama-action,demonstrating the system’s reliability and ability to learn human activity from drone videos.展开更多
The Neuro-Immuno-Endocrine axis coordinates interactions between the nervous, immune, and endocrine systems to maintain physiological homeostasis and respond to internal and external stimuli. Dysregulation of this axi...The Neuro-Immuno-Endocrine axis coordinates interactions between the nervous, immune, and endocrine systems to maintain physiological homeostasis and respond to internal and external stimuli. Dysregulation of this axis is implicated in chronic stress, inflammation, and a variety of diseases. Zen meditation, a focused and intentional practice rooted in mindfulness, has gained recognition for its profound health benefits. By engaging the Neuro-Immuno-Endocrine axis, Zen meditation supports stress reduction, immune resilience, and hormonal balance. This review explores the interplay between Zen meditation and the Neuro-Immuno-Endocrine axis, emphasizing its neurological, immune, and endocrine impacts. A research question guiding this review is: “How does Zen meditation influence the interconnected components of the Neuro-Immuno-Endocrine axis, and what are the potential mechanisms underlying these effects?” This review hypothesizes that Zen meditation positively impacts the Neuro-Immuno-Endocrine axis through stress-reduction mechanisms that enhance neuroplasticity, reduce systemic inflammation, and balance hormonal pathways. We further discuss how the synergy between Zen meditation and the Neuro-Immuno-Endocrine axis contributes to holistic health and suggest future research directions to optimize its therapeutic applications.展开更多
In this study, we are first examining well-known approach to improve fuzzy reasoning model (FRM) by use of the genetic-based learning mechanism [1]. Later we propose our alternative way to build FRM, which has signifi...In this study, we are first examining well-known approach to improve fuzzy reasoning model (FRM) by use of the genetic-based learning mechanism [1]. Later we propose our alternative way to build FRM, which has significant precision advantages and does not require any adjustment/learning. We put together neuro-fuzzy system (NFS) to connect the set of exemplar input feature vectors (FV) with associated output label (target), both represented by their membership functions (MF). Next unknown FV would be classified by getting upper value of current output MF. After that the fuzzy truths for all MF upper values are maximized and the label of the winner is considered as the class of the input FV. We use the knowledge in the exemplar-label pairs directly with no training. It sets up automatically and then classifies all input FV from the same population as the exemplar FVs. We show that our approach statistically is almost twice as accurate, as well-known genetic-based learning mechanism FRM.展开更多
The effects of low-doses of microcystin-leucinearginine （ MC-LR ） exposure on neurobehaviors and N-acetylaspartate （NAA） expression in the hippocampus of rats were investigated. After male Sprague-Dawley （SD） ra...The effects of low-doses of microcystin-leucinearginine （ MC-LR ） exposure on neurobehaviors and N-acetylaspartate （NAA） expression in the hippocampus of rats were investigated. After male Sprague-Dawley （SD） rats were treated intra-gastrically with different doses of MC-LR for 90 d, the locomotor activity, spatial learning and memory function were evaluated in the rats after treatment using open field tests and Morris water maze tests. The results show that MC-LR exposure can lead to impairment of the spatial learning capacity and locomotor activity in rats at the dose of 2. 00 p,g/kg. The levels of NAA in the hippocampus were measured by magnetic resonance spectroscopy （MRI）. A significant decrease of NAA/Cr ratio （ P 〈 0. 05） was observed in the hippocampous. This study indicates that intra-gastrical exposure to low-doses of MC-LR has adverse effects on neuronal behavior and NAA levels in the hippocampous.展开更多
Objective:Glioblastoma(GBM)is the most prevalent and aggressive adult primary cancer in the central nervous system.Therapeutic approaches for GBM treatment are under intense investigation,including the use of emerging...Objective:Glioblastoma(GBM)is the most prevalent and aggressive adult primary cancer in the central nervous system.Therapeutic approaches for GBM treatment are under intense investigation,including the use of emerging immunotherapies.Here,we propose an alternative approach to treat GBM through reprogramming proliferative GBM cells into non-proliferative neurons.Methods:Retroviruses were used to target highly proliferative human GBM cells through overexpression of neural transcription factors.Immunostaining,electrophysiological recording,and bulk RNA-seq were performed to investigate the mechanisms underlying the neuronal conversion of human GBM cells.An in vivo intracranial xenograft mouse model was used to examine the neuronal conversion of human GBM cells.Results:We report efficient neuronal conversion from human GBM cells by overexpressing single neural transcription factor Neurogenic differentiation 1(Neuro D1),Neurogenin-2(Neurog2),or Achaete-scute homolog 1(Ascl1).Subtype characterization showed that the majority of Neurog2-and Neuro D1-converted neurons were glutamatergic,while Ascl1 favored GABAergic neuron generation.The GBM cell-converted neurons not only showed pan-neuronal markers but also exhibited neuron-specific electrophysiological activities.Transcriptome analyses revealed that neuronal genes were activated in glioma cells after overexpression of neural transcription factors,and different signaling pathways were activated by different neural transcription factors.Importantly,the neuronal conversion of GBM cells was accompanied by significant inhibition of GBM cell proliferation in both in vitro and in vivo models.Conclusions:These results suggest that GBM cells can be reprogrammed into different subtypes of neurons,leading to a potential alternative approach to treat brain tumors using in vivo cell conversion technology.展开更多
Neurodegenerative eye diseases, such as glaucoma, cause irreversible vision loss in millions of patients worldwide, creating serious medical, economic and social issues. Like other mammalian central nervous system tra...Neurodegenerative eye diseases, such as glaucoma, cause irreversible vision loss in millions of patients worldwide, creating serious medical, economic and social issues. Like other mammalian central nervous system tracts, optic nerve intrinsically lacks the capacity for axonal growth and its surrounding environment is also non-permissive to regeneration. Any axonal damage also triggers a vicious cycle of retinal ganglion cell （RGC） death. Exploring methods that can enhance RGCs survival and promote axonal regeneration will not only enable vision restoration for millions of patients, but also shed light on the treatment of other neurodegenerative diseases. In this review article, we will go through three current approaches to cure neu- rodegenerative eye diseases, including cell based therapy, neuro-regeneration and neuro-rejuvenation.展开更多
Two kinds of neuro-fuzzy gust response alleviation control laws are designed for a flexible large-aspect-ratio wing model. Simulations and comparisons of random gust alleviation using the two control laws are performe...Two kinds of neuro-fuzzy gust response alleviation control laws are designed for a flexible large-aspect-ratio wing model. Simulations and comparisons of random gust alleviation using the two control laws are performed. Based on the better neuro-fuzzy control law,experiments and simulations of sinusoidal gust alleviation using one-control-surface control system and two-control-surface control system are developed. The investigations show that the two kinds of neuro-fuzzy gust response alleviation control laws can alleviate random gust responses effectively. The neuro-fuzzy gust response alleviation control law including a modifying factor is better than the other one without it. Further,the better one has good effects on the sinusoidal gust alleviation at different frequencies and flow velocities. The two-control-surface control system has better effects on gust response alleviation than the one-control-surface control system when the gust is strong. The simulation results agree well with the experimental results. These results can be usefully referenced to the design of actual gust alleviation control systems.展开更多
目的优化LipofectamineTM2000介导外源基因转染Neuro-2a(N-2a)细胞的条件,以提高转染效率。方法将携带增强型绿色荧光蛋白(Enhanced green fluorescent protein,EGFP)的真核表达载体pEGFP-N1在LipofectamineTM2000介导下转染N-2a细胞,...目的优化LipofectamineTM2000介导外源基因转染Neuro-2a(N-2a)细胞的条件,以提高转染效率。方法将携带增强型绿色荧光蛋白(Enhanced green fluorescent protein,EGFP)的真核表达载体pEGFP-N1在LipofectamineTM2000介导下转染N-2a细胞,对转染时间、细胞融合度、质粒质量与脂质体体积比例及培养基pH值进行优化,荧光显微镜观察EGFP的表达,并计算转染效率。结果在细胞融合度达80%,质粒质量与脂质体体积比为1∶3的条件下转染48 h,EGFP的阳性率最高(P<0.05);培养基pH值为8.0左右时,EGFP的阳性率较高,且细胞凋亡率较低。结论优化了LipofectamineTM2000转染N-2a细胞的条件,提高了转染效率,为外源基因高效转染N-2a细胞奠定了基础。展开更多
Large cell neuroendocrine carcinoma (LCNEC) in the biliary system is a poorly differentiated, high-grade neuroendocrine tumor. These tumors exhibit aggressive behavior and an increased tendency for early nodal and dis...Large cell neuroendocrine carcinoma (LCNEC) in the biliary system is a poorly differentiated, high-grade neuroendocrine tumor. These tumors exhibit aggressive behavior and an increased tendency for early nodal and distant metastases. Herein, we report an unusual case of a pure primary LCNEC of the common bile duct (CBD). A 75-year-old female presented with nausea and jaundice. The patient underwent a CBD excision with lymph node dissection. Upon histological and immunohistochemical examination, the tumor exhibited pure large cell-type neuroendocrine features. Metastases were noted in two of the eight lymph nodes. The patient was administered adjuvant chemotherapy. The patient’s cancer recurred 7 mo after surgery, and the patient died from liver failure 5 mo after recurrence. The prognosis of LCNEC of CBD remains poor despite curative resection and adjuvant chemotherapy. The role of additional therapies, such as multimodal treatment including radiation therapy, must be further studied to improve the prognoses of patients.展开更多
基金funded by the Open Access Initiative of the University of Bremen and the DFG via SuUB Bremen.Princess Nourah bint Abdulrahman University Researchers Supporting Project number(PNURSP2024R348),Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia.
文摘Human Activity Recognition(HAR)in drone-captured videos has become popular because of the interest in various fields such as video surveillance,sports analysis,and human-robot interaction.However,recognizing actions from such videos poses the following challenges:variations of human motion,the complexity of backdrops,motion blurs,occlusions,and restricted camera angles.This research presents a human activity recognition system to address these challenges by working with drones’red-green-blue(RGB)videos.The first step in the proposed system involves partitioning videos into frames and then using bilateral filtering to improve the quality of object foregrounds while reducing background interference before converting from RGB to grayscale images.The YOLO(You Only Look Once)algorithm detects and extracts humans from each frame,obtaining their skeletons for further processing.The joint angles,displacement and velocity,histogram of oriented gradients(HOG),3D points,and geodesic Distance are included.These features are optimized using Quadratic Discriminant Analysis(QDA)and utilized in a Neuro-Fuzzy Classifier(NFC)for activity classification.Real-world evaluations on the Drone-Action,Unmanned Aerial Vehicle(UAV)-Gesture,and Okutama-Action datasets substantiate the proposed system’s superiority in accuracy rates over existing methods.In particular,the system obtains recognition rates of 93%for drone action,97%for UAV gestures,and 81%for Okutama-action,demonstrating the system’s reliability and ability to learn human activity from drone videos.
文摘The Neuro-Immuno-Endocrine axis coordinates interactions between the nervous, immune, and endocrine systems to maintain physiological homeostasis and respond to internal and external stimuli. Dysregulation of this axis is implicated in chronic stress, inflammation, and a variety of diseases. Zen meditation, a focused and intentional practice rooted in mindfulness, has gained recognition for its profound health benefits. By engaging the Neuro-Immuno-Endocrine axis, Zen meditation supports stress reduction, immune resilience, and hormonal balance. This review explores the interplay between Zen meditation and the Neuro-Immuno-Endocrine axis, emphasizing its neurological, immune, and endocrine impacts. A research question guiding this review is: “How does Zen meditation influence the interconnected components of the Neuro-Immuno-Endocrine axis, and what are the potential mechanisms underlying these effects?” This review hypothesizes that Zen meditation positively impacts the Neuro-Immuno-Endocrine axis through stress-reduction mechanisms that enhance neuroplasticity, reduce systemic inflammation, and balance hormonal pathways. We further discuss how the synergy between Zen meditation and the Neuro-Immuno-Endocrine axis contributes to holistic health and suggest future research directions to optimize its therapeutic applications.
文摘In this study, we are first examining well-known approach to improve fuzzy reasoning model (FRM) by use of the genetic-based learning mechanism [1]. Later we propose our alternative way to build FRM, which has significant precision advantages and does not require any adjustment/learning. We put together neuro-fuzzy system (NFS) to connect the set of exemplar input feature vectors (FV) with associated output label (target), both represented by their membership functions (MF). Next unknown FV would be classified by getting upper value of current output MF. After that the fuzzy truths for all MF upper values are maximized and the label of the winner is considered as the class of the input FV. We use the knowledge in the exemplar-label pairs directly with no training. It sets up automatically and then classifies all input FV from the same population as the exemplar FVs. We show that our approach statistically is almost twice as accurate, as well-known genetic-based learning mechanism FRM.
基金The National Natural Science Foundation of China(No.8107225881273123)
文摘The effects of low-doses of microcystin-leucinearginine （ MC-LR ） exposure on neurobehaviors and N-acetylaspartate （NAA） expression in the hippocampus of rats were investigated. After male Sprague-Dawley （SD） rats were treated intra-gastrically with different doses of MC-LR for 90 d, the locomotor activity, spatial learning and memory function were evaluated in the rats after treatment using open field tests and Morris water maze tests. The results show that MC-LR exposure can lead to impairment of the spatial learning capacity and locomotor activity in rats at the dose of 2. 00 p,g/kg. The levels of NAA in the hippocampus were measured by magnetic resonance spectroscopy （MRI）. A significant decrease of NAA/Cr ratio （ P 〈 0. 05） was observed in the hippocampous. This study indicates that intra-gastrical exposure to low-doses of MC-LR has adverse effects on neuronal behavior and NAA levels in the hippocampous.
基金supported by the Charles H.“Skip”Smith Endowment Fund and the Verne M.Willaman Endowment Fund from the Pennsylvania State University to G.C。
文摘Objective:Glioblastoma(GBM)is the most prevalent and aggressive adult primary cancer in the central nervous system.Therapeutic approaches for GBM treatment are under intense investigation,including the use of emerging immunotherapies.Here,we propose an alternative approach to treat GBM through reprogramming proliferative GBM cells into non-proliferative neurons.Methods:Retroviruses were used to target highly proliferative human GBM cells through overexpression of neural transcription factors.Immunostaining,electrophysiological recording,and bulk RNA-seq were performed to investigate the mechanisms underlying the neuronal conversion of human GBM cells.An in vivo intracranial xenograft mouse model was used to examine the neuronal conversion of human GBM cells.Results:We report efficient neuronal conversion from human GBM cells by overexpressing single neural transcription factor Neurogenic differentiation 1(Neuro D1),Neurogenin-2(Neurog2),or Achaete-scute homolog 1(Ascl1).Subtype characterization showed that the majority of Neurog2-and Neuro D1-converted neurons were glutamatergic,while Ascl1 favored GABAergic neuron generation.The GBM cell-converted neurons not only showed pan-neuronal markers but also exhibited neuron-specific electrophysiological activities.Transcriptome analyses revealed that neuronal genes were activated in glioma cells after overexpression of neural transcription factors,and different signaling pathways were activated by different neural transcription factors.Importantly,the neuronal conversion of GBM cells was accompanied by significant inhibition of GBM cell proliferation in both in vitro and in vivo models.Conclusions:These results suggest that GBM cells can be reprogrammed into different subtypes of neurons,leading to a potential alternative approach to treat brain tumors using in vivo cell conversion technology.
基金supported by the National Glaucoma Research Program of the Bright Focus Foundationsupported by an unrestricted research grant from Research to Prevent BlindnessNIH center grant EY014801
文摘Neurodegenerative eye diseases, such as glaucoma, cause irreversible vision loss in millions of patients worldwide, creating serious medical, economic and social issues. Like other mammalian central nervous system tracts, optic nerve intrinsically lacks the capacity for axonal growth and its surrounding environment is also non-permissive to regeneration. Any axonal damage also triggers a vicious cycle of retinal ganglion cell （RGC） death. Exploring methods that can enhance RGCs survival and promote axonal regeneration will not only enable vision restoration for millions of patients, but also shed light on the treatment of other neurodegenerative diseases. In this review article, we will go through three current approaches to cure neu- rodegenerative eye diseases, including cell based therapy, neuro-regeneration and neuro-rejuvenation.
基金National Natural Science Foundation of China(90716006)
文摘Two kinds of neuro-fuzzy gust response alleviation control laws are designed for a flexible large-aspect-ratio wing model. Simulations and comparisons of random gust alleviation using the two control laws are performed. Based on the better neuro-fuzzy control law,experiments and simulations of sinusoidal gust alleviation using one-control-surface control system and two-control-surface control system are developed. The investigations show that the two kinds of neuro-fuzzy gust response alleviation control laws can alleviate random gust responses effectively. The neuro-fuzzy gust response alleviation control law including a modifying factor is better than the other one without it. Further,the better one has good effects on the sinusoidal gust alleviation at different frequencies and flow velocities. The two-control-surface control system has better effects on gust response alleviation than the one-control-surface control system when the gust is strong. The simulation results agree well with the experimental results. These results can be usefully referenced to the design of actual gust alleviation control systems.
文摘目的优化LipofectamineTM2000介导外源基因转染Neuro-2a(N-2a)细胞的条件,以提高转染效率。方法将携带增强型绿色荧光蛋白(Enhanced green fluorescent protein,EGFP)的真核表达载体pEGFP-N1在LipofectamineTM2000介导下转染N-2a细胞,对转染时间、细胞融合度、质粒质量与脂质体体积比例及培养基pH值进行优化,荧光显微镜观察EGFP的表达,并计算转染效率。结果在细胞融合度达80%,质粒质量与脂质体体积比为1∶3的条件下转染48 h,EGFP的阳性率最高(P<0.05);培养基pH值为8.0左右时,EGFP的阳性率较高,且细胞凋亡率较低。结论优化了LipofectamineTM2000转染N-2a细胞的条件,提高了转染效率,为外源基因高效转染N-2a细胞奠定了基础。
文摘Large cell neuroendocrine carcinoma (LCNEC) in the biliary system is a poorly differentiated, high-grade neuroendocrine tumor. These tumors exhibit aggressive behavior and an increased tendency for early nodal and distant metastases. Herein, we report an unusual case of a pure primary LCNEC of the common bile duct (CBD). A 75-year-old female presented with nausea and jaundice. The patient underwent a CBD excision with lymph node dissection. Upon histological and immunohistochemical examination, the tumor exhibited pure large cell-type neuroendocrine features. Metastases were noted in two of the eight lymph nodes. The patient was administered adjuvant chemotherapy. The patient’s cancer recurred 7 mo after surgery, and the patient died from liver failure 5 mo after recurrence. The prognosis of LCNEC of CBD remains poor despite curative resection and adjuvant chemotherapy. The role of additional therapies, such as multimodal treatment including radiation therapy, must be further studied to improve the prognoses of patients.
