The immunomodulatory and anti-oxidative activities of differentiated mesenchymal stem cells contribute to their therapeutic efficacy in cell-replacement therapy. Mesenchymal stem cells were isolated from human umbilic...The immunomodulatory and anti-oxidative activities of differentiated mesenchymal stem cells contribute to their therapeutic efficacy in cell-replacement therapy. Mesenchymal stem cells were isolated from human umbilical cord and induced to differentiate with basic fibroblast growth factor, nerve growth factor, epidermal growth factor, brain-derived neurotrophic factor and forskolin. The mesenchymal stem cells became rounded with long processes and expressed the neural markers, Tujl, neurofilament 200, microtubule-associated protein-2 and neuron-specific enolase. Nestin expression was significantly reduced after neural induction. The expression of immunoregulatory and anti-oxidative genes was largely unchanged prior to and after neural induction in mesenchymal stem cells. There was no significant difference in the effects of control and induced mesenchymal stem cells on lymphocyte proliferation in co-culture experiments. However, the expression of human leukocyte antigen-G decreased significantly in induced neuron-like cells. These results suggest that growth factor-based methods enable the differentiation of mesenchymal stem cell toward immature neuronal-like cells, which retain their immunomodulatory and anti-oxidative activities.展开更多
Cell couplings before and after neural induction in embryos of Cynops orientalis were studied by means of single cell injection of Lucifer Yellow.Differences both in incidence and the extent of cell couplings were dem...Cell couplings before and after neural induction in embryos of Cynops orientalis were studied by means of single cell injection of Lucifer Yellow.Differences both in incidence and the extent of cell couplings were demonstrated.Results of cell couplings were correlated with electron microscopic observations of freeze-etching replicas.展开更多
Some studies indicate that adipose derived stem cells(ADSCs)can differentiate into adipogenic,chondrogenic,myogenic,and osteogenic cells in vitro.However,whether ADSCs can be induced to differentiate into neural cells...Some studies indicate that adipose derived stem cells(ADSCs)can differentiate into adipogenic,chondrogenic,myogenic,and osteogenic cells in vitro.However,whether ADSCs can be induced to differentiate into neural cells in vitro has not been clearly demonstrated.In this study,the ADSCs isolated from the murine adipose tissue were cultured and transfected with the EGFP gene,and then the cells were induced for neural differentiation.The morphology of those ADSCs began to change within two days which developed i...展开更多
Mesenchymal stem cells (MSCs) are self-renewing cells found in almost all <span>postnatal organs and tissues in the perivascular region. These cells present</span> multiple characteristics that make them c...Mesenchymal stem cells (MSCs) are self-renewing cells found in almost all <span>postnatal organs and tissues in the perivascular region. These cells present</span> multiple characteristics that make them candidates to be applied in cell therapy for neurodegenerative diseases, such as their secretory action, migration to the lesion area, and immunomodulatory potential. These cells have a high <span>capacity for mesodermal differentiation;however, numerous studies have</span> shown that MSCs can also differentiate into neurons. However, despite posi<span>tive results in multiple trials in which undifferentiated MSCs transplanted</span> into animal models of neurodegenerative diseases, some studies suggest that the therapeutic effects obtained are enhanced by the use of MSCs differentiated towards the neuronal lineage before transplant. In this sense, there are <span>several methods to induce <i>in vitro</i> reprogramming of MSCs towards the</span> neuronal lineage, including chemical substances, growth factors, cocultures with neural lineage cells, transfection of genes, miRNAs, etc., and small molecules <span>stand out. Therefore, this article compares multiple experimental tests in </span>which these inducers promote neuronal differentiation of MSCs and identify those methods that originate an optimal neuronal differentiation. The analysis includes the percentage of differentiation, maturation, expression of neuronal markers, functionality, and cell survival considering the intrinsic characteristics of the MSCs used as the tissue of origin and the species from which they were isolated.展开更多
基金supported by grants from the Shandong Province Science and Technology Program, GrantNo.2011GSF11801the Innovation Fund Project of Shandong University, Grant No.2012ZD023the Major StateBasic Research Development Program, Grant No.2012CB966504
文摘The immunomodulatory and anti-oxidative activities of differentiated mesenchymal stem cells contribute to their therapeutic efficacy in cell-replacement therapy. Mesenchymal stem cells were isolated from human umbilical cord and induced to differentiate with basic fibroblast growth factor, nerve growth factor, epidermal growth factor, brain-derived neurotrophic factor and forskolin. The mesenchymal stem cells became rounded with long processes and expressed the neural markers, Tujl, neurofilament 200, microtubule-associated protein-2 and neuron-specific enolase. Nestin expression was significantly reduced after neural induction. The expression of immunoregulatory and anti-oxidative genes was largely unchanged prior to and after neural induction in mesenchymal stem cells. There was no significant difference in the effects of control and induced mesenchymal stem cells on lymphocyte proliferation in co-culture experiments. However, the expression of human leukocyte antigen-G decreased significantly in induced neuron-like cells. These results suggest that growth factor-based methods enable the differentiation of mesenchymal stem cell toward immature neuronal-like cells, which retain their immunomodulatory and anti-oxidative activities.
文摘Cell couplings before and after neural induction in embryos of Cynops orientalis were studied by means of single cell injection of Lucifer Yellow.Differences both in incidence and the extent of cell couplings were demonstrated.Results of cell couplings were correlated with electron microscopic observations of freeze-etching replicas.
基金supported by the "Eleventh Five-Year" plan to support the National Science and Technology key project(No.2007BAI04B07)a grant from National Natural Sciences Foundation of China(No.30772206)
文摘Some studies indicate that adipose derived stem cells(ADSCs)can differentiate into adipogenic,chondrogenic,myogenic,and osteogenic cells in vitro.However,whether ADSCs can be induced to differentiate into neural cells in vitro has not been clearly demonstrated.In this study,the ADSCs isolated from the murine adipose tissue were cultured and transfected with the EGFP gene,and then the cells were induced for neural differentiation.The morphology of those ADSCs began to change within two days which developed i...
文摘Mesenchymal stem cells (MSCs) are self-renewing cells found in almost all <span>postnatal organs and tissues in the perivascular region. These cells present</span> multiple characteristics that make them candidates to be applied in cell therapy for neurodegenerative diseases, such as their secretory action, migration to the lesion area, and immunomodulatory potential. These cells have a high <span>capacity for mesodermal differentiation;however, numerous studies have</span> shown that MSCs can also differentiate into neurons. However, despite posi<span>tive results in multiple trials in which undifferentiated MSCs transplanted</span> into animal models of neurodegenerative diseases, some studies suggest that the therapeutic effects obtained are enhanced by the use of MSCs differentiated towards the neuronal lineage before transplant. In this sense, there are <span>several methods to induce <i>in vitro</i> reprogramming of MSCs towards the</span> neuronal lineage, including chemical substances, growth factors, cocultures with neural lineage cells, transfection of genes, miRNAs, etc., and small molecules <span>stand out. Therefore, this article compares multiple experimental tests in </span>which these inducers promote neuronal differentiation of MSCs and identify those methods that originate an optimal neuronal differentiation. The analysis includes the percentage of differentiation, maturation, expression of neuronal markers, functionality, and cell survival considering the intrinsic characteristics of the MSCs used as the tissue of origin and the species from which they were isolated.
