Objectives:This study aimed to assess the reliability and validity of the abbreviated Committed Action Questionnaire(CAQ-8)in a cohort of 1635 Chinese university students.Methods:Participants completed the Chinese ver...Objectives:This study aimed to assess the reliability and validity of the abbreviated Committed Action Questionnaire(CAQ-8)in a cohort of 1635 Chinese university students.Methods:Participants completed the Chinese version of the CAQ-8 along with other standardized measures,including the Acceptance and Action Questionnaire-II(AAQ-II),the Valuing Questionnaire(VQ),the Satisfaction with Life Scale(SWLS),the Depression Anxiety Stress Scales(DASS-21),and the World Health Organization Fiveitem Well-Being Index(WHO-5).A retest was conducted one month later with 300 valid responses.Results:Exploratory factor analysis(n=818)identified a 2-factor structure,confirmed through validated factor analysis(n=817),showing good fit indices(CFI=0.990,RMSEA=0.040).Measurement equivalence across genders was established.The CAQ-8 showed significant positive correlations with life satisfaction,mental health,and values,and negative correlations with depression,anxiety,stress,and experiential avoidance.The scale demonstrated good internal consistency(Cronbach’sα=0.76)and retest reliability(ICC=0.70).Network analysis confirmed the robustness of the 2-factor model,with item 4 in CAQ-8 identified as a core item.Conclusion:The CAQ-8 is a reliable and valid tool for measuring committed action within the psychological flexibility model in Chinese populations.展开更多
Traditional Chinese medicine(TCM)has played a significant role in the prevention and treatment of chronic heart failure(CHF).To study TCM diagnosis of CHF,a total of 278 Chinese clinical research articles on the study...Traditional Chinese medicine(TCM)has played a significant role in the prevention and treatment of chronic heart failure(CHF).To study TCM diagnosis of CHF,a total of 278 Chinese clinical research articles on the study of CHF syndromes in recent 40 years retrieved from Web of Science,Scopus,Pub Med,Embase,CNKI,Wanfang Data,Cq VIP,and Sino Med.According to cumulative frequency analysis,network analysis,and hierarchical cluster analysis,the study found the distribution of CHF syndromes was syndrome of qi deficiency with blood stasis,syndrome of qi and yin deficiency,syndrome of yang deficiency with water flooding,syndrome of heart blood stasis obstruction,syndrome of turbid phlegm,and syndrome of collapse due to primordial yang deficiency.The syndrome elements on location of illness were heart,kidney,lung,and spleen.The syndrome elements on nature of illness were qi deficiency,blood stasis,yang deficiency,yin deficiency,water retention,and turbid phlegm.These findings can provide reference to the research on diagnosis and treatment of CHF,and contribute to the study on syndrome standardization and objective research of TCM diagnosis.展开更多
Objectives To identify core symptoms and symptom clusters in patients with neuromyelitis optica spectrum disorder(NMOSD)by network analysis.Methods From October 10 to 30,2023,140 patients with NMOSD were selected to p...Objectives To identify core symptoms and symptom clusters in patients with neuromyelitis optica spectrum disorder(NMOSD)by network analysis.Methods From October 10 to 30,2023,140 patients with NMOSD were selected to participate in this online questionnaire survey.The survey tools included a general information questionnaire and a self-made NMOSD symptoms scale,which included the prevalence,severity,and distress of 29 symptoms.Cluster analysis was used to identify symptom clusters,and network analysis was used to analyze the symptom network and node characteristics and central indicators including strength centrality(r_(s)),closeness centrality(r_(c))and betweeness centrality(r_(b))were used to identify core symptoms and symptom clusters.Results The most common symptom was pain(65.7%),followed by paraesthesia(65.0%),fatigue(65.0%),easy awakening(63.6%).Regarding the burden level of symptoms,pain was the most burdensome symptom,followed by paraesthesia,easy awakening,fatigue,and difficulty falling asleep.Six clusters were identified:somatosensory,motor,visual,and memory symptom clusters,bladder and rectum symptom clusters,sleep symptoms clusters,and neuropsychological symptom clusters.Fatigue(r_(s)=12.39,r_(b)=68.00,r_(c)=0.02)was the most central and prominent bridge symptom,and motor symptom cluster(r_(s)=2.68,r_(c)=0.10)was the most central symptom cluster among the six clusters.Conclusions Our study demonstrated the necessity of symptom management targeting fatigue,pain,and motor symptom cluster in patients with NMOSD.展开更多
The construction of“park cities”requires a systematic thinking to coordinate the networked development of park system and break through the limitation of emphasizing scale and grade and neglecting dynamic correlatio...The construction of“park cities”requires a systematic thinking to coordinate the networked development of park system and break through the limitation of emphasizing scale and grade and neglecting dynamic correlation in traditional planning.Taking Haidian District of Beijing as an example,the social network analysis method is introduced to construct the network model of park green spaces.Through indicators such as clustering coefficient,network density and node centrality,the characteristics of its spatial structure and hierarchical relationship are analyzed.It is found that the network integrity presents the characteristics of“highly local concentration and global fragmentation”,fragmented park green space network and missing spatial connection,isolated clusters and collaborative failure,as well as the spatial mismatch between population and resource supply and demand.Hierarchical issues include“structural imbalance and functional disorder”,disorder between network hierarchy and park level,misalignment of functional hierarchy leading to weakened network risk resistance capacity,and a relatively dense distribution of core nodes,etc.In response to the above problems,a multi-level spatial intervention strategy should be adopted to solve the overall problem of the network.Meanwhile,it is needed to clarify the positioning of a park itself and improve the hierarchical system,so as to construct a multi-level and multi-scale park green space network,contribute to the construction of a park city,and provide residents with more diverse activity venues.展开更多
BACKGROUND Depression and anxiety are prevalent psychological challenges among patients with adolescent idiopathic scoliosis(AIS),affecting individuals across both sex and age groups.AIM To explore the network structu...BACKGROUND Depression and anxiety are prevalent psychological challenges among patients with adolescent idiopathic scoliosis(AIS),affecting individuals across both sex and age groups.AIM To explore the network structure of depression and anxiety symptoms,with a focus on identifying differences at the symptom level between sex and age subgroups.METHODS A total of 1955 participants diagnosed with AIS aged 10-18 years were assessed using the Patient Health Questionnaire Depression Scale(PHO-9)and the Generalized Anxiety Disorder Scale(GAD-7),and 765 patients exhibiting PHQ-9 or GAD-7 scores ≥ 5 were enrolled in our study. Network analysis and network comparison tests were utilized toconstruct and compare the depression-anxiety symptoms networks among sex and age subgroups.RESULTSThe results revealed GAD3 “Excessive worry” and PHQ2 “Sad mood” were the most significant central symptomsin all subgroups, while “Sad mood” had higher strength than “Excessive worry” in the lower age group. In thenetwork comparisons, the female network exhibited tighter connectivity, especially on GAD6 “Irritability” andGAD2 “Uncontrollable worry”, while only PHQ3 “Sleep” and PHQ9 “Suicidal ideation” had differences at thelocal level in the lower age group.CONCLUSIONSeveral interventions targeting excessive worry and sad mood could reduce the risk of depression and anxietysymptoms in the AIS population. Furthermore, specific anxiety symptoms in females, along with sleep disturbancesand suicidal ideation in the lower age group, should be addressed at an early stage to prevent significantdisruptions in mental health trajectories.展开更多
Immune changes and inflammatory responses have been identified as central events in the pathological process of spinal co rd injury.They can greatly affect nerve regeneration and functional recovery.However,there is s...Immune changes and inflammatory responses have been identified as central events in the pathological process of spinal co rd injury.They can greatly affect nerve regeneration and functional recovery.However,there is still limited understanding of the peripheral immune inflammato ry response in spinal cord inju ry.In this study.we obtained microRNA expression profiles from the peripheral blood of patients with spinal co rd injury using high-throughput sequencing.We also obtained the mRNA expression profile of spinal cord injury patients from the Gene Expression Omnibus(GEO)database(GSE151371).We identified 54 differentially expressed microRNAs and 1656 diffe rentially expressed genes using bioinformatics approaches.Functional enrichment analysis revealed that various common immune and inflammation-related signaling pathways,such as neutrophil extracellular trap formation pathway,T cell receptor signaling pathway,and nuclear factor-κB signal pathway,we re abnormally activated or inhibited in spinal cord inju ry patient samples.We applied an integrated strategy that combines weighted gene co-expression network analysis,LASSO logistic regression,and SVM-RFE algorithm and identified three biomarke rs associated with spinal cord injury:ANO10,BST1,and ZFP36L2.We verified the expression levels and diagnostic perfo rmance of these three genes in the original training dataset and clinical samples through the receiver operating characteristic curve.Quantitative polymerase chain reaction results showed that ANO20 and BST1 mRNA levels were increased and ZFP36L2 mRNA was decreased in the peripheral blood of spinal cord injury patients.We also constructed a small RNA-mRNA interaction network using Cytoscape.Additionally,we evaluated the proportion of 22 types of immune cells in the peripheral blood of spinal co rd injury patients using the CIBERSORT tool.The proportions of naive B cells,plasma cells,monocytes,and neutrophils were increased while the proportions of memory B cells,CD8^(+)T cells,resting natural killer cells,resting dendritic cells,and eosinophils were markedly decreased in spinal cord injury patients increased compared with healthy subjects,and ANO10,BST1 and ZFP26L2we re closely related to the proportion of certain immune cell types.The findings from this study provide new directions for the development of treatment strategies related to immune inflammation in spinal co rd inju ry and suggest that ANO10,BST2,and ZFP36L2 are potential biomarkers for spinal cord injury.The study was registe red in the Chinese Clinical Trial Registry(registration No.ChiCTR2200066985,December 12,2022).展开更多
BACKGROUND Non-suicidal self-injury(NSSI)is common among adolescents and frequently cooccurs with depression.Understanding the distinct patterns of NSSI behaviors,along with their associated risk and protective factor...BACKGROUND Non-suicidal self-injury(NSSI)is common among adolescents and frequently cooccurs with depression.Understanding the distinct patterns of NSSI behaviors,along with their associated risk and protective factors,is crucial for developing effective interventions.AIM To classify NSSI behaviors and examine interactions between risk and resilience factors in Chinese adolescents.METHODS A cross-sectional study involving 3967 Chinese students(51.7%female,mean age 13.58±2.24 years)who completed questionnaires on parenting styles,bullying,childhood maltreatment,depression,resilience,and NSSI.Latent profile analysis(LPA)was used to identify NSSI subtypes,and network analysis explored interactions between risk and resilience factors.RESULTS Three NSSI subtypes were identified:NSSI with depression(18.8%),NSSI without depression(12.3%),and neither(68.9%).Bullying was the central risk factor across subtypes,while emotional control and family support were key protective factors.Statistical analyses showed significant differences between groups(P<0.001).CONCLUSION This study identified three NSSI subtypes among Chinese adolescents.Bullying emerged as a central risk factor,while emotional control and family support were key protective factors.Targeting these areas may help reduce NSSI behaviors in this population.展开更多
Objective:Network analysis was used to explore the complex inter-relationships between social participation activities and depressive symptoms among the Chinese older population,and the differences in network structur...Objective:Network analysis was used to explore the complex inter-relationships between social participation activities and depressive symptoms among the Chinese older population,and the differences in network structures among different genders,age groups,and urban-rural residency would be compared.Methods:Based on the 2018 wave of the Chinese Longitudinal Healthy Longevity Survey(CLHLS),12,043 people aged 65 to 105 were included.The 10-item Center for Epidemiologic Studies Depression(CESD)Scale was used to assess depressive symptoms and 10 types of social participation activities were collected,including housework,tai-chi,square dancing,visiting and interacting with friends,garden work,reading newspapers or books,raising domestic animals,playing cards or mahjong,watching TV or listening to radio,and organized social activities.R 4.2.1 software was used to estimate the network model and calculate strength and bridge strength.Results:21.60%(2,601/12,043)of the participants had depressive symptoms.The total social participation score was negatively associated with depressive symptoms after adjusting for sociodemographic factors.The network of social participation and depressive symptoms showed that“D9(Inability to get going)”and“S9(Watching TV and/or listening to the radio)”had the highest strength within depressive symptoms and social participation communities,respectively,and“S1(Housework)”,“S9(Watching TV and/or listening to the radio)”,and“D5(Hopelessness)”were the most prominent bridging nodes between the two communities.Most edges linking the two communities were negative.“S5(Graden work)-D5(Hopelessness)”and“S6(Reading newspapers/books)-D4(Everything was an effort)”were the top 2 strongest negative edges.Older females had significantly denser network structures than older males.Compared to older people aged 65e80,the age group 81e105 showed higher network global strength.Conclusions:This study provides novel insights into the complex relationships between social participation and depressive symptoms.Except for doing housework,other social participation activities were found to be protective for depression levels.Different nursing strategies should be taken to prevent and alleviate depressive symptoms for different genders and older people of different ages.展开更多
If progress is to be made toward improving geohazard management and emergency decision-making,then lessons need to be learned from past geohazard information.A geologic hazard report provides a useful and reliable sou...If progress is to be made toward improving geohazard management and emergency decision-making,then lessons need to be learned from past geohazard information.A geologic hazard report provides a useful and reliable source of information about the occurrence of an event,along with detailed information about the condition or factors of the geohazard.Analyzing such reports,however,can be a challenging process because these texts are often presented in unstructured long text formats,and contain rich specialized and detailed information.Automatically text classification is commonly used to mine disaster text data in open domains(e.g.,news and microblogs).But it has limitations to performing contextual long-distance dependencies and is insensitive to discourse order.These deficiencies are most obviously exposed in long text fields.Therefore,this paper uses the bidirectional encoder representations from Transformers(BERT),to model long text.Then,utilizing a softmax layer to automatically extract text features and classify geohazards without manual features.The latent Dirichlet allocation(LDA)model is used to examine the interdependencies that exist between causal variables to visualize geohazards.The proposed method is useful in enabling the machine-assisted interpretation of text-based geohazards.Moreover,it can help users visualize causes,processes,and other geohazards and assist decision-makers in emergency responses.展开更多
Purpose:We analyzed the structure of a community of authors working in the field of social network analysis(SNA)based on citation indicators:direct citation and bibliographic coupling metrics.We observed patterns at t...Purpose:We analyzed the structure of a community of authors working in the field of social network analysis(SNA)based on citation indicators:direct citation and bibliographic coupling metrics.We observed patterns at the micro,meso,and macro levels of analysis.Design/methodology/approach:We used bibliometric network analysis,including the“temporal quantities”approach proposed to study temporal networks.Using a two-mode network linking publications with authors and a one-mode network of citations between the works,we constructed and analyzed the networks of citation and bibliographic coupling among authors.We used an iterated saturation data collection approach.Findings:At the macro-level,we observed the global structural features of citations between authors,showing that 80%of authors have not more than 15 citations from other works.At the meso-level,we extracted the groups of authors citing each other and similar to each other according to their citation patterns.We have seen a division of authors in SNA into groups of social scientists and physicists,as well as into other groups of authors from different disciplines.We found some examples of brokerage between different groups that maintained the common identity of the field.At the micro-level,we extracted authors with extremely high values of received citations,who can be considered as the most prominent authors in the field.We examined the temporal properties of the most popular authors.Research limitations:The main challenge in this approach is the resolution of the author’s name(synonyms and homonyms).We faced the author disambiguation,or“multiple personalities”(Harzing,2015)problem.To remain consistent and comparable with our previously published articles,we used the same SNA data collected up to 2018.The analysis and conclusions on the activity,productivity,and visibility of the authors are relative only to the field of SNA.Practical implications:The proposed approach can be utilized for similar objectives and identifying key structures and characteristics in other disciplines.This may potentially inspire the application of network approaches in other research areas,creating more authors collaborating in the field of SNA.Originality/value:We identified and applied an innovative approach and methods to study the structure of scientific communities,which allowed us to get the findings going beyond those obtained with other methods.We used a new approach to temporal network analysis,which is an important addition to the analysis as it provides detailed information on different measures for the authors and pairs of authors over time.展开更多
Research in Information Science and interdisciplinary areas suggested the formation of a growing network of international research collaboration.The massive transmission of COVID-19 worldwide especially after the iden...Research in Information Science and interdisciplinary areas suggested the formation of a growing network of international research collaboration.The massive transmission of COVID-19 worldwide especially after the identification of the Omicron variant could fundamentally alter the factors shaping the network's development.This study employs network analysis methods to analyze the structure of the COVID-19 research collaboration from 2020 to 2022,using two major academic publication databases and the VOSviewer software.A novel temporal view is added by examining the dynamic changes of the network,and a fractional counting method is adopted as methodological improvements to previous research.Analysis reveals that the COVID-19 research network structure has undergone substantial changes over time,as collaborating countries and regions form and re-form new clusters.Transformations in the network can be partly explained by key developments in the pandemic and other social-political events.China as one of the largest pivots in the network formed a relatively distinct cluster,with potential to develop a larger Asia-Pacific collaboration cluster based on its research impact.展开更多
Esophageal cancer is a common malignant tumor, whose pathogenesis and prognosis factors are not fully understood. This study aimed to discover the gene clusters that have similar functions and can be used to predict t...Esophageal cancer is a common malignant tumor, whose pathogenesis and prognosis factors are not fully understood. This study aimed to discover the gene clusters that have similar functions and can be used to predict the prognosis of esophageal cancer. The matched microarray and RNA sequencing data of 185 patients with esophageal cancer were downloaded from The Cancer Genome Atlas(TCGA), and gene co-expression networks were built without distinguishing between squamous carcinoma and adenocarcinoma. The result showed that 12 modules were associated with one or more survival data such as recurrence status, recurrence time, vital status or vital time. Furthermore, survival analysis showed that 5 out of the 12 modules were related to progression-free survival(PFS) or overall survival(OS). As the most important module, the midnight blue module with 82 genes was related to PFS, apart from the patient age, tumor grade, primary treatment success, and duration of smoking and tumor histological type. Gene ontology enrichment analysis revealed that 'glycoprotein binding' was the top enriched function of midnight blue module genes. Additionally, the blue module was the exclusive gene clusters related to OS. Platelet activating factor receptor(PTAFR) and feline Gardner-Rasheed(FGR) were the top hub genes in both modeling datasets and the STRING protein interaction database. In conclusion, our study provides novel insights into the prognosis-associated genes and screens out candidate biomarkers for esophageal cancer.展开更多
Axon regeneration is crucial for recovery from neurological diseases. Numerous studies have identified several genes, microRNAs （miRNAs）, and transcription factors （TFs） that influence axon regeneration. However, ...Axon regeneration is crucial for recovery from neurological diseases. Numerous studies have identified several genes, microRNAs （miRNAs）, and transcription factors （TFs） that influence axon regeneration. However, the regulatory networks involved have not been fully elucidated. In the present study, we analyzed a regulatory network of 51 miRNAs, 27 TFs, and 59 target genes, which is involved in axon regeneration. We identified 359 pairs of feed- forward loops （FFLs）, seven important genes （Naplll, Arhgef12, Sema6d, Akt3, Trim2, Rabllfip2, and Rps6ka3）, six important miRNAs （hsa-miR-204-5p, hsa-miR-124-3p, hsa-miR-26a-5p, hsa-miR-16-5p, hsa-miR-17-5p, and hsa- miR-15b-5p）, and eight important TFs （Smada2, Flil, Wtl, Sp6, Sp3, Smad4, Smad5, and Crebl）, which appear to play an important role in axon regeneration. Functional enrichment analysis revealed that axon-associated genes are involved mainly in the regulation of cellular component organization, axonogenesis, and cell morphogenesis during neuronal differentiation. However, these findings need to be validated by further studies.展开更多
AIM: To identify and understand the relationship between co-expression pattern and clinic traits in uveal melanoma, weighted gene co-expression network analysis(WGCNA) is applied to investigate the gene expression lev...AIM: To identify and understand the relationship between co-expression pattern and clinic traits in uveal melanoma, weighted gene co-expression network analysis(WGCNA) is applied to investigate the gene expression levels and patient clinic features. Uveal melanoma is the most common primary eye tumor in adults. Although many studies have identified some important genes and pathways that were relevant to progress of uveal melanoma, the relationship between co-expression and clinic traits in systems level of uveal melanoma is unclear yet. We employ WGCNA to investigate the relationship underlying molecular and phenotype in this study.METHODS: Gene expression profile of uveal melanoma and patient clinic traits were collected from the Gene Expression Omnibus(GEO) database. The gene co-expression is calculated by WGCNA that is the R package software. The package is used to analyze the correlation between pairs of expression levels of genes.The function of the genes were annotated by gene ontology(GO).RESULTS: In this study, we identified four co-expression modules significantly correlated with clinictraits. Module blue positively correlated with radiotherapy treatment. Module purple positively correlates with tumor location(sclera) and negatively correlates with patient age. Module red positively correlates with sclera and negatively correlates with thickness of tumor. Module black positively correlates with the largest tumor diameter(LTD). Additionally, we identified the hug gene(top connectivity with other genes) in each module. The hub gene RPS15 A, PTGDS, CD53 and MSI2 might play a vital role in progress of uveal melanoma.CONCLUSION: From WGCNA analysis and hub gene calculation, we identified RPS15 A, PTGDS, CD53 and MSI2 might be target or diagnosis for uveal melanoma.展开更多
Peripheral nerve injury repair requires a certain degree of cooperation between axon regeneration and Wallerian degeneration.Therefore,investigating how axon regeneration and degeneration work together to repair perip...Peripheral nerve injury repair requires a certain degree of cooperation between axon regeneration and Wallerian degeneration.Therefore,investigating how axon regeneration and degeneration work together to repair peripheral nerve injury may uncover the molecular mechanisms and signal cascades underlying peripheral nerve repair and provide potential strategies for improving the low axon regeneration capacity of the central nervous system.In this study,we applied weighted gene co-expression network analysis to identify differentially expressed genes in proximal and distal sciatic nerve segments from rats with sciatic nerve injury.We identified 31 and 15 co-expression modules from the proximal and distal sciatic nerve segments,respectively.Functional enrichment analysis revealed that the differentially expressed genes in proximal modules promoted regeneration,while the differentially expressed genes in distal modules promoted neurodegeneration.Next,we constructed hub gene networks for selected modules and identified a key hub gene,Kif22,which was up-regulated in both nerve segments.In vitro experiments confirmed that Kif22 knockdown inhibited proliferation and migration of Schwann cells by modulating the activity of the extracellular signal-regulated kinase signaling pathway.Collectively,our findings provide a comparative framework of gene modules that are co-expressed in injured proximal and distal sciatic nerve segments,and identify Kif22 as a potential therapeutic target for promoting peripheral nerve injury repair via Schwann cell proliferation and migration.All animal experiments were approved by the Institutional Animal Ethics Committee of Nantong University,China(approval No.S20210322-008)on March 22,2021.展开更多
To meet the challenge of sustainable development, sustainability must be made. Ecological network analysis(ENA) was introduced in this paper as an approach to quantitatively measure the growth, development, and sustai...To meet the challenge of sustainable development, sustainability must be made. Ecological network analysis(ENA) was introduced in this paper as an approach to quantitatively measure the growth, development, and sustainability of an economic system. The Guangdong economic networks from 1987 to 2010 were analyzed by applying the ENA approach. Firstly, a currency flow network among economic sectors was constructed to represent the Guangdong economic system by adapting the input-output(I-O) table data. Then, the network indicators from the ENA framework involving the total system throughput(TST), average mutual information(AMI), ascendency(A), redundancy(R) and development capacity(C) were calculated. Lastly, the network indicators were analyzed to acquire the overall features of Guangdong's economic operations during 1987–2010. The results are as follows: the trends of the network indicators show that the size of the Guangdong economic network grows exponentially at a high rate during 1987–2010, whereas its efficiency does not present a clear trend over its whole period. The growth is the main characteristic of the Guangdong economy during 1987–2010, with no clear evidence regarding its development. The quantitative results of the network also confirmed that the growth contributed to a great majority of the Guangdong economy during 1987–2010, whereas the development's contribution was tiny during the same period. The average value of the sustainability indicator(α) of the Guangdong economic network was 0.222 during 1987–2010, which is less than the theoretically optimal value of 0.37 for a sustainable human-influenced system. The results suggest that the Guangdong economic system needs a further autocatalysis to improve its efficiency to support the system maintaining a sustainable evolvement.展开更多
The frequent occurrence of geopolitical crises in the post-financial crisis era is driving the rethinking behind whether the global crude oil market is still a highly connected"great pool".Using the spillove...The frequent occurrence of geopolitical crises in the post-financial crisis era is driving the rethinking behind whether the global crude oil market is still a highly connected"great pool".Using the spillover network model suggested by Baruník and Krehlík(2018),and the daily data of 31 global crude oil markets from 2009 to 2019,this study examines the return and volatility spillover effects and their timevarying behavior in six crude oil market segments at different timescales.The findings indicate that heterogeneity exists in the co-movements between global crude oil markets in the post-financial crisis era.In the medium term,both return and volatility spillover effects are not significant,which makes the diversified portfolio strategy useful.Prices in the Europe and Central Asian regions take the lead in return spillovers.In contrast,Asia-Pacific regional prices contribute the most in terms of volatility spillovers.Long-term volatility spillovers increase sharply when confronted with oil-related events in the postfinancial crisis era.Therefore,policymakers should take effective measures to prevent any large-scale risk transmission in the long run.展开更多
Study of gene expression has been arguably the most active research field in functional genomics.Over the last two decades,various high-throughput technologies,from gene expression microarray to RNA-seq,have been wide...Study of gene expression has been arguably the most active research field in functional genomics.Over the last two decades,various high-throughput technologies,from gene expression microarray to RNA-seq,have been widely applied to the wholegenome profiling of gene expression.The commonality of these experiments is that they measure the gene expression levels of"bulk"sample,which pools a large number(often in the scale of millions)of cells,and thus the measurements reflect the average expression展开更多
Objective Alzheimer's disease(AD)is the most common cause of dementia.The pathophysiology of the disease mostly remains unearthed,thereby challenging drug development for AD.This study aims to screen high throughp...Objective Alzheimer's disease(AD)is the most common cause of dementia.The pathophysiology of the disease mostly remains unearthed,thereby challenging drug development for AD.This study aims to screen high throughput gene expression data using weighted co-expression network analysis(WGCNA)to explore the potential therapeutic targets.Methods The dataset of GSE36980 was obtained from the Gene Expression Omnibus(GEO)database.Normalization,quality control,filtration,and soft-threshold calculation were carried out before clustering the co-expressed genes into different modules.Furthermore,the correlation coefiidents between the modules and clinical traits were computed to identify the key modules.Gene ontology and pathway enrichment analyses were performed on the key module genes.The STRING database was used to construct the protein-protein interaction(PPI)networks,which were further analyzed by Cytoscape app(MCODE).Finally,validation of hub genes was conducted by external GEO datasets of GSE 1297 and GSE 28146.Results Co-expressed genes were clustered into 27 modules,among which 6 modules were identified as the key module relating to AD occurrence.These key modules are primarily involved in chemical synaptic transmission(G0:0007268),the tricarboxylic acid(TCA)cycle and respiratory electron transport(R-HSA-1428517).WDR47,OXCT1,C3orfl4,ATP6V1A,SLC25A14,NAPB were found as the hub genes and their expression were validated by external datasets.Conclusions Through modules co-expression network analyses and PPI network analyses,we identified the hub genes of AD,including WDR47,0XCT1,C3orfl4i ATP6V1A,SLC25A14 and NAPB.Among them,three hub genes(ATP6V1A,SLC25A14,OXCT1)might contribute to AD pathogenesis through pathway of TCA cycle.展开更多
The tool system of the organizational risk analyzer (ORA) to study the network of East Turkistan terrorists is selected. The model of the relationships among its personnel, knowledge, resources and task entities is re...The tool system of the organizational risk analyzer (ORA) to study the network of East Turkistan terrorists is selected. The model of the relationships among its personnel, knowledge, resources and task entities is represented by the meta-matrix in ORA, with which to analyze the risks and vulnerabilities of organizational structure quantitatively, and obtain the last vulnerabilities and risks of the organization. Case study in this system shows that it should be a shortcut to destroy effectively the network of terrorists by recognizing the caucus persons of the terrorism organization for the first and eliminating them when strikes the terror organization. It is vital to ensure effective use of the resources and control the risks of terrorist attacks.展开更多
文摘Objectives:This study aimed to assess the reliability and validity of the abbreviated Committed Action Questionnaire(CAQ-8)in a cohort of 1635 Chinese university students.Methods:Participants completed the Chinese version of the CAQ-8 along with other standardized measures,including the Acceptance and Action Questionnaire-II(AAQ-II),the Valuing Questionnaire(VQ),the Satisfaction with Life Scale(SWLS),the Depression Anxiety Stress Scales(DASS-21),and the World Health Organization Fiveitem Well-Being Index(WHO-5).A retest was conducted one month later with 300 valid responses.Results:Exploratory factor analysis(n=818)identified a 2-factor structure,confirmed through validated factor analysis(n=817),showing good fit indices(CFI=0.990,RMSEA=0.040).Measurement equivalence across genders was established.The CAQ-8 showed significant positive correlations with life satisfaction,mental health,and values,and negative correlations with depression,anxiety,stress,and experiential avoidance.The scale demonstrated good internal consistency(Cronbach’sα=0.76)and retest reliability(ICC=0.70).Network analysis confirmed the robustness of the 2-factor model,with item 4 in CAQ-8 identified as a core item.Conclusion:The CAQ-8 is a reliable and valid tool for measuring committed action within the psychological flexibility model in Chinese populations.
基金financed by the grants from the National Natural Science Foundation of China(No.81803996)Shanghai Key Laboratory of Health Identification and Assessment(No.21DZ2271000)。
文摘Traditional Chinese medicine(TCM)has played a significant role in the prevention and treatment of chronic heart failure(CHF).To study TCM diagnosis of CHF,a total of 278 Chinese clinical research articles on the study of CHF syndromes in recent 40 years retrieved from Web of Science,Scopus,Pub Med,Embase,CNKI,Wanfang Data,Cq VIP,and Sino Med.According to cumulative frequency analysis,network analysis,and hierarchical cluster analysis,the study found the distribution of CHF syndromes was syndrome of qi deficiency with blood stasis,syndrome of qi and yin deficiency,syndrome of yang deficiency with water flooding,syndrome of heart blood stasis obstruction,syndrome of turbid phlegm,and syndrome of collapse due to primordial yang deficiency.The syndrome elements on location of illness were heart,kidney,lung,and spleen.The syndrome elements on nature of illness were qi deficiency,blood stasis,yang deficiency,yin deficiency,water retention,and turbid phlegm.These findings can provide reference to the research on diagnosis and treatment of CHF,and contribute to the study on syndrome standardization and objective research of TCM diagnosis.
基金supported by the Specific Research Fund for Top-notch Talents of Guangdong Provincial Hospital of Chinese Medicine(No.2022KT1188).
文摘Objectives To identify core symptoms and symptom clusters in patients with neuromyelitis optica spectrum disorder(NMOSD)by network analysis.Methods From October 10 to 30,2023,140 patients with NMOSD were selected to participate in this online questionnaire survey.The survey tools included a general information questionnaire and a self-made NMOSD symptoms scale,which included the prevalence,severity,and distress of 29 symptoms.Cluster analysis was used to identify symptom clusters,and network analysis was used to analyze the symptom network and node characteristics and central indicators including strength centrality(r_(s)),closeness centrality(r_(c))and betweeness centrality(r_(b))were used to identify core symptoms and symptom clusters.Results The most common symptom was pain(65.7%),followed by paraesthesia(65.0%),fatigue(65.0%),easy awakening(63.6%).Regarding the burden level of symptoms,pain was the most burdensome symptom,followed by paraesthesia,easy awakening,fatigue,and difficulty falling asleep.Six clusters were identified:somatosensory,motor,visual,and memory symptom clusters,bladder and rectum symptom clusters,sleep symptoms clusters,and neuropsychological symptom clusters.Fatigue(r_(s)=12.39,r_(b)=68.00,r_(c)=0.02)was the most central and prominent bridge symptom,and motor symptom cluster(r_(s)=2.68,r_(c)=0.10)was the most central symptom cluster among the six clusters.Conclusions Our study demonstrated the necessity of symptom management targeting fatigue,pain,and motor symptom cluster in patients with NMOSD.
文摘The construction of“park cities”requires a systematic thinking to coordinate the networked development of park system and break through the limitation of emphasizing scale and grade and neglecting dynamic correlation in traditional planning.Taking Haidian District of Beijing as an example,the social network analysis method is introduced to construct the network model of park green spaces.Through indicators such as clustering coefficient,network density and node centrality,the characteristics of its spatial structure and hierarchical relationship are analyzed.It is found that the network integrity presents the characteristics of“highly local concentration and global fragmentation”,fragmented park green space network and missing spatial connection,isolated clusters and collaborative failure,as well as the spatial mismatch between population and resource supply and demand.Hierarchical issues include“structural imbalance and functional disorder”,disorder between network hierarchy and park level,misalignment of functional hierarchy leading to weakened network risk resistance capacity,and a relatively dense distribution of core nodes,etc.In response to the above problems,a multi-level spatial intervention strategy should be adopted to solve the overall problem of the network.Meanwhile,it is needed to clarify the positioning of a park itself and improve the hierarchical system,so as to construct a multi-level and multi-scale park green space network,contribute to the construction of a park city,and provide residents with more diverse activity venues.
基金Supported by The Sanming Project of Medicine in Shenzhen,No.SZSM202211003Shenzhen-Hong Kong Jointly Funded Project,Shenzhen Science and Technology Program,No.SGDX20230116093645007+1 种基金Shenzhen Second People's Hospital Clinical Project,No.20243357003Shenzhen Medical Research Fund,No.B2303005.
文摘BACKGROUND Depression and anxiety are prevalent psychological challenges among patients with adolescent idiopathic scoliosis(AIS),affecting individuals across both sex and age groups.AIM To explore the network structure of depression and anxiety symptoms,with a focus on identifying differences at the symptom level between sex and age subgroups.METHODS A total of 1955 participants diagnosed with AIS aged 10-18 years were assessed using the Patient Health Questionnaire Depression Scale(PHO-9)and the Generalized Anxiety Disorder Scale(GAD-7),and 765 patients exhibiting PHQ-9 or GAD-7 scores ≥ 5 were enrolled in our study. Network analysis and network comparison tests were utilized toconstruct and compare the depression-anxiety symptoms networks among sex and age subgroups.RESULTSThe results revealed GAD3 “Excessive worry” and PHQ2 “Sad mood” were the most significant central symptomsin all subgroups, while “Sad mood” had higher strength than “Excessive worry” in the lower age group. In thenetwork comparisons, the female network exhibited tighter connectivity, especially on GAD6 “Irritability” andGAD2 “Uncontrollable worry”, while only PHQ3 “Sleep” and PHQ9 “Suicidal ideation” had differences at thelocal level in the lower age group.CONCLUSIONSeveral interventions targeting excessive worry and sad mood could reduce the risk of depression and anxietysymptoms in the AIS population. Furthermore, specific anxiety symptoms in females, along with sleep disturbancesand suicidal ideation in the lower age group, should be addressed at an early stage to prevent significantdisruptions in mental health trajectories.
基金supported by the Notional Natural Science Foundation of China,No.81960417 (to JX)Guangxi Key Research and Development Program,No.GuiKeA B20159027 (to JX)the Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.2022GXNSFBA035545 (to YG)。
文摘Immune changes and inflammatory responses have been identified as central events in the pathological process of spinal co rd injury.They can greatly affect nerve regeneration and functional recovery.However,there is still limited understanding of the peripheral immune inflammato ry response in spinal cord inju ry.In this study.we obtained microRNA expression profiles from the peripheral blood of patients with spinal co rd injury using high-throughput sequencing.We also obtained the mRNA expression profile of spinal cord injury patients from the Gene Expression Omnibus(GEO)database(GSE151371).We identified 54 differentially expressed microRNAs and 1656 diffe rentially expressed genes using bioinformatics approaches.Functional enrichment analysis revealed that various common immune and inflammation-related signaling pathways,such as neutrophil extracellular trap formation pathway,T cell receptor signaling pathway,and nuclear factor-κB signal pathway,we re abnormally activated or inhibited in spinal cord inju ry patient samples.We applied an integrated strategy that combines weighted gene co-expression network analysis,LASSO logistic regression,and SVM-RFE algorithm and identified three biomarke rs associated with spinal cord injury:ANO10,BST1,and ZFP36L2.We verified the expression levels and diagnostic perfo rmance of these three genes in the original training dataset and clinical samples through the receiver operating characteristic curve.Quantitative polymerase chain reaction results showed that ANO20 and BST1 mRNA levels were increased and ZFP36L2 mRNA was decreased in the peripheral blood of spinal cord injury patients.We also constructed a small RNA-mRNA interaction network using Cytoscape.Additionally,we evaluated the proportion of 22 types of immune cells in the peripheral blood of spinal co rd injury patients using the CIBERSORT tool.The proportions of naive B cells,plasma cells,monocytes,and neutrophils were increased while the proportions of memory B cells,CD8^(+)T cells,resting natural killer cells,resting dendritic cells,and eosinophils were markedly decreased in spinal cord injury patients increased compared with healthy subjects,and ANO10,BST1 and ZFP26L2we re closely related to the proportion of certain immune cell types.The findings from this study provide new directions for the development of treatment strategies related to immune inflammation in spinal co rd inju ry and suggest that ANO10,BST2,and ZFP36L2 are potential biomarkers for spinal cord injury.The study was registe red in the Chinese Clinical Trial Registry(registration No.ChiCTR2200066985,December 12,2022).
基金Supported by Yunnan Province High-Level Health Technical Talents,Leading Talents,No.L-2019011.
文摘BACKGROUND Non-suicidal self-injury(NSSI)is common among adolescents and frequently cooccurs with depression.Understanding the distinct patterns of NSSI behaviors,along with their associated risk and protective factors,is crucial for developing effective interventions.AIM To classify NSSI behaviors and examine interactions between risk and resilience factors in Chinese adolescents.METHODS A cross-sectional study involving 3967 Chinese students(51.7%female,mean age 13.58±2.24 years)who completed questionnaires on parenting styles,bullying,childhood maltreatment,depression,resilience,and NSSI.Latent profile analysis(LPA)was used to identify NSSI subtypes,and network analysis explored interactions between risk and resilience factors.RESULTS Three NSSI subtypes were identified:NSSI with depression(18.8%),NSSI without depression(12.3%),and neither(68.9%).Bullying was the central risk factor across subtypes,while emotional control and family support were key protective factors.Statistical analyses showed significant differences between groups(P<0.001).CONCLUSION This study identified three NSSI subtypes among Chinese adolescents.Bullying emerged as a central risk factor,while emotional control and family support were key protective factors.Targeting these areas may help reduce NSSI behaviors in this population.
基金supported by the National Key Research and Development Plan Project(grant number:2022YFC3600904)The funding organization had no role in the survey’s design,implementation,and analysis.
文摘Objective:Network analysis was used to explore the complex inter-relationships between social participation activities and depressive symptoms among the Chinese older population,and the differences in network structures among different genders,age groups,and urban-rural residency would be compared.Methods:Based on the 2018 wave of the Chinese Longitudinal Healthy Longevity Survey(CLHLS),12,043 people aged 65 to 105 were included.The 10-item Center for Epidemiologic Studies Depression(CESD)Scale was used to assess depressive symptoms and 10 types of social participation activities were collected,including housework,tai-chi,square dancing,visiting and interacting with friends,garden work,reading newspapers or books,raising domestic animals,playing cards or mahjong,watching TV or listening to radio,and organized social activities.R 4.2.1 software was used to estimate the network model and calculate strength and bridge strength.Results:21.60%(2,601/12,043)of the participants had depressive symptoms.The total social participation score was negatively associated with depressive symptoms after adjusting for sociodemographic factors.The network of social participation and depressive symptoms showed that“D9(Inability to get going)”and“S9(Watching TV and/or listening to the radio)”had the highest strength within depressive symptoms and social participation communities,respectively,and“S1(Housework)”,“S9(Watching TV and/or listening to the radio)”,and“D5(Hopelessness)”were the most prominent bridging nodes between the two communities.Most edges linking the two communities were negative.“S5(Graden work)-D5(Hopelessness)”and“S6(Reading newspapers/books)-D4(Everything was an effort)”were the top 2 strongest negative edges.Older females had significantly denser network structures than older males.Compared to older people aged 65e80,the age group 81e105 showed higher network global strength.Conclusions:This study provides novel insights into the complex relationships between social participation and depressive symptoms.Except for doing housework,other social participation activities were found to be protective for depression levels.Different nursing strategies should be taken to prevent and alleviate depressive symptoms for different genders and older people of different ages.
基金supported by the Natural Science Foundation of China(No.42301492)the National Key Research and Development Program(No.2022YFB3904200)+4 种基金the Open Fund of Key Laboratory of Urban Land Resources Monitoring and Simulation,Ministry of Natural Resources(No.KF-2022-07-014)the Natural Science Foundation of Hubei Province of China(No.2022CFB640)the Open Fund of Hubei Key Laboratory of Intelligent Vision Based Monitoring for Hydroelectric Engineering(No.2022SDSJ04)the Opening Fund of Key Laboratory of Geological Survey and Evaluation of Ministry of Education(No.GLAB 2023ZR01)the Fundamental Research Funds for the Central Universities.
文摘If progress is to be made toward improving geohazard management and emergency decision-making,then lessons need to be learned from past geohazard information.A geologic hazard report provides a useful and reliable source of information about the occurrence of an event,along with detailed information about the condition or factors of the geohazard.Analyzing such reports,however,can be a challenging process because these texts are often presented in unstructured long text formats,and contain rich specialized and detailed information.Automatically text classification is commonly used to mine disaster text data in open domains(e.g.,news and microblogs).But it has limitations to performing contextual long-distance dependencies and is insensitive to discourse order.These deficiencies are most obviously exposed in long text fields.Therefore,this paper uses the bidirectional encoder representations from Transformers(BERT),to model long text.Then,utilizing a softmax layer to automatically extract text features and classify geohazards without manual features.The latent Dirichlet allocation(LDA)model is used to examine the interdependencies that exist between causal variables to visualize geohazards.The proposed method is useful in enabling the machine-assisted interpretation of text-based geohazards.Moreover,it can help users visualize causes,processes,and other geohazards and assist decision-makers in emergency responses.
基金supported in part by the Slovenian Research Agency(VB,research program P1-0294)(VB,research project J5-2557)+2 种基金(VB,research project J5-4596)COST EU(VB,COST action CA21163(HiTEc)is prepared within the framework of the HSE University Basic Research Program.
文摘Purpose:We analyzed the structure of a community of authors working in the field of social network analysis(SNA)based on citation indicators:direct citation and bibliographic coupling metrics.We observed patterns at the micro,meso,and macro levels of analysis.Design/methodology/approach:We used bibliometric network analysis,including the“temporal quantities”approach proposed to study temporal networks.Using a two-mode network linking publications with authors and a one-mode network of citations between the works,we constructed and analyzed the networks of citation and bibliographic coupling among authors.We used an iterated saturation data collection approach.Findings:At the macro-level,we observed the global structural features of citations between authors,showing that 80%of authors have not more than 15 citations from other works.At the meso-level,we extracted the groups of authors citing each other and similar to each other according to their citation patterns.We have seen a division of authors in SNA into groups of social scientists and physicists,as well as into other groups of authors from different disciplines.We found some examples of brokerage between different groups that maintained the common identity of the field.At the micro-level,we extracted authors with extremely high values of received citations,who can be considered as the most prominent authors in the field.We examined the temporal properties of the most popular authors.Research limitations:The main challenge in this approach is the resolution of the author’s name(synonyms and homonyms).We faced the author disambiguation,or“multiple personalities”(Harzing,2015)problem.To remain consistent and comparable with our previously published articles,we used the same SNA data collected up to 2018.The analysis and conclusions on the activity,productivity,and visibility of the authors are relative only to the field of SNA.Practical implications:The proposed approach can be utilized for similar objectives and identifying key structures and characteristics in other disciplines.This may potentially inspire the application of network approaches in other research areas,creating more authors collaborating in the field of SNA.Originality/value:We identified and applied an innovative approach and methods to study the structure of scientific communities,which allowed us to get the findings going beyond those obtained with other methods.We used a new approach to temporal network analysis,which is an important addition to the analysis as it provides detailed information on different measures for the authors and pairs of authors over time.
基金the Decision and Consultancy Research of Shanghai Jiao Tong University(No.JCZXZGB-01)。
文摘Research in Information Science and interdisciplinary areas suggested the formation of a growing network of international research collaboration.The massive transmission of COVID-19 worldwide especially after the identification of the Omicron variant could fundamentally alter the factors shaping the network's development.This study employs network analysis methods to analyze the structure of the COVID-19 research collaboration from 2020 to 2022,using two major academic publication databases and the VOSviewer software.A novel temporal view is added by examining the dynamic changes of the network,and a fractional counting method is adopted as methodological improvements to previous research.Analysis reveals that the COVID-19 research network structure has undergone substantial changes over time,as collaborating countries and regions form and re-form new clusters.Transformations in the network can be partly explained by key developments in the pandemic and other social-political events.China as one of the largest pivots in the network formed a relatively distinct cluster,with potential to develop a larger Asia-Pacific collaboration cluster based on its research impact.
文摘Esophageal cancer is a common malignant tumor, whose pathogenesis and prognosis factors are not fully understood. This study aimed to discover the gene clusters that have similar functions and can be used to predict the prognosis of esophageal cancer. The matched microarray and RNA sequencing data of 185 patients with esophageal cancer were downloaded from The Cancer Genome Atlas(TCGA), and gene co-expression networks were built without distinguishing between squamous carcinoma and adenocarcinoma. The result showed that 12 modules were associated with one or more survival data such as recurrence status, recurrence time, vital status or vital time. Furthermore, survival analysis showed that 5 out of the 12 modules were related to progression-free survival(PFS) or overall survival(OS). As the most important module, the midnight blue module with 82 genes was related to PFS, apart from the patient age, tumor grade, primary treatment success, and duration of smoking and tumor histological type. Gene ontology enrichment analysis revealed that 'glycoprotein binding' was the top enriched function of midnight blue module genes. Additionally, the blue module was the exclusive gene clusters related to OS. Platelet activating factor receptor(PTAFR) and feline Gardner-Rasheed(FGR) were the top hub genes in both modeling datasets and the STRING protein interaction database. In conclusion, our study provides novel insights into the prognosis-associated genes and screens out candidate biomarkers for esophageal cancer.
基金Project supported by the Key Project of Hebei North University(No.120177)the Science and Technology Bureau Research Development Plan of Zhangjiakou City in Hebei(No.0911021D-4)China
文摘Axon regeneration is crucial for recovery from neurological diseases. Numerous studies have identified several genes, microRNAs （miRNAs）, and transcription factors （TFs） that influence axon regeneration. However, the regulatory networks involved have not been fully elucidated. In the present study, we analyzed a regulatory network of 51 miRNAs, 27 TFs, and 59 target genes, which is involved in axon regeneration. We identified 359 pairs of feed- forward loops （FFLs）, seven important genes （Naplll, Arhgef12, Sema6d, Akt3, Trim2, Rabllfip2, and Rps6ka3）, six important miRNAs （hsa-miR-204-5p, hsa-miR-124-3p, hsa-miR-26a-5p, hsa-miR-16-5p, hsa-miR-17-5p, and hsa- miR-15b-5p）, and eight important TFs （Smada2, Flil, Wtl, Sp6, Sp3, Smad4, Smad5, and Crebl）, which appear to play an important role in axon regeneration. Functional enrichment analysis revealed that axon-associated genes are involved mainly in the regulation of cellular component organization, axonogenesis, and cell morphogenesis during neuronal differentiation. However, these findings need to be validated by further studies.
基金Supported by the National Natural Science Foundation of China(No.81271019No.61463046)Gansu Province Science Foundation for Youths(No.145RJYA282)
文摘AIM: To identify and understand the relationship between co-expression pattern and clinic traits in uveal melanoma, weighted gene co-expression network analysis(WGCNA) is applied to investigate the gene expression levels and patient clinic features. Uveal melanoma is the most common primary eye tumor in adults. Although many studies have identified some important genes and pathways that were relevant to progress of uveal melanoma, the relationship between co-expression and clinic traits in systems level of uveal melanoma is unclear yet. We employ WGCNA to investigate the relationship underlying molecular and phenotype in this study.METHODS: Gene expression profile of uveal melanoma and patient clinic traits were collected from the Gene Expression Omnibus(GEO) database. The gene co-expression is calculated by WGCNA that is the R package software. The package is used to analyze the correlation between pairs of expression levels of genes.The function of the genes were annotated by gene ontology(GO).RESULTS: In this study, we identified four co-expression modules significantly correlated with clinictraits. Module blue positively correlated with radiotherapy treatment. Module purple positively correlates with tumor location(sclera) and negatively correlates with patient age. Module red positively correlates with sclera and negatively correlates with thickness of tumor. Module black positively correlates with the largest tumor diameter(LTD). Additionally, we identified the hug gene(top connectivity with other genes) in each module. The hub gene RPS15 A, PTGDS, CD53 and MSI2 might play a vital role in progress of uveal melanoma.CONCLUSION: From WGCNA analysis and hub gene calculation, we identified RPS15 A, PTGDS, CD53 and MSI2 might be target or diagnosis for uveal melanoma.
基金supported by the National Major Project of Research and Development of China,No.2017YFA0104701(to BY)the National Natural Science Foundation of China,No.32000725(to QQC)+1 种基金the Natural Science Foundation of Jiangsu Province of China,No.BK20200973(to QQC)the Jiangsu Provincial University Innovation Training Key Project of China,No.202010304021Z(to ML)。
文摘Peripheral nerve injury repair requires a certain degree of cooperation between axon regeneration and Wallerian degeneration.Therefore,investigating how axon regeneration and degeneration work together to repair peripheral nerve injury may uncover the molecular mechanisms and signal cascades underlying peripheral nerve repair and provide potential strategies for improving the low axon regeneration capacity of the central nervous system.In this study,we applied weighted gene co-expression network analysis to identify differentially expressed genes in proximal and distal sciatic nerve segments from rats with sciatic nerve injury.We identified 31 and 15 co-expression modules from the proximal and distal sciatic nerve segments,respectively.Functional enrichment analysis revealed that the differentially expressed genes in proximal modules promoted regeneration,while the differentially expressed genes in distal modules promoted neurodegeneration.Next,we constructed hub gene networks for selected modules and identified a key hub gene,Kif22,which was up-regulated in both nerve segments.In vitro experiments confirmed that Kif22 knockdown inhibited proliferation and migration of Schwann cells by modulating the activity of the extracellular signal-regulated kinase signaling pathway.Collectively,our findings provide a comparative framework of gene modules that are co-expressed in injured proximal and distal sciatic nerve segments,and identify Kif22 as a potential therapeutic target for promoting peripheral nerve injury repair via Schwann cell proliferation and migration.All animal experiments were approved by the Institutional Animal Ethics Committee of Nantong University,China(approval No.S20210322-008)on March 22,2021.
基金Under the auspices of the Fundamental Research Funds for the Central Universities,China(No.2015KJJCB30)
文摘To meet the challenge of sustainable development, sustainability must be made. Ecological network analysis(ENA) was introduced in this paper as an approach to quantitatively measure the growth, development, and sustainability of an economic system. The Guangdong economic networks from 1987 to 2010 were analyzed by applying the ENA approach. Firstly, a currency flow network among economic sectors was constructed to represent the Guangdong economic system by adapting the input-output(I-O) table data. Then, the network indicators from the ENA framework involving the total system throughput(TST), average mutual information(AMI), ascendency(A), redundancy(R) and development capacity(C) were calculated. Lastly, the network indicators were analyzed to acquire the overall features of Guangdong's economic operations during 1987–2010. The results are as follows: the trends of the network indicators show that the size of the Guangdong economic network grows exponentially at a high rate during 1987–2010, whereas its efficiency does not present a clear trend over its whole period. The growth is the main characteristic of the Guangdong economy during 1987–2010, with no clear evidence regarding its development. The quantitative results of the network also confirmed that the growth contributed to a great majority of the Guangdong economy during 1987–2010, whereas the development's contribution was tiny during the same period. The average value of the sustainability indicator(α) of the Guangdong economic network was 0.222 during 1987–2010, which is less than the theoretically optimal value of 0.37 for a sustainable human-influenced system. The results suggest that the Guangdong economic system needs a further autocatalysis to improve its efficiency to support the system maintaining a sustainable evolvement.
基金the financial support from the National Natural Science Foundation of China(No.71922013)。
文摘The frequent occurrence of geopolitical crises in the post-financial crisis era is driving the rethinking behind whether the global crude oil market is still a highly connected"great pool".Using the spillover network model suggested by Baruník and Krehlík(2018),and the daily data of 31 global crude oil markets from 2009 to 2019,this study examines the return and volatility spillover effects and their timevarying behavior in six crude oil market segments at different timescales.The findings indicate that heterogeneity exists in the co-movements between global crude oil markets in the post-financial crisis era.In the medium term,both return and volatility spillover effects are not significant,which makes the diversified portfolio strategy useful.Prices in the Europe and Central Asian regions take the lead in return spillovers.In contrast,Asia-Pacific regional prices contribute the most in terms of volatility spillovers.Long-term volatility spillovers increase sharply when confronted with oil-related events in the postfinancial crisis era.Therefore,policymakers should take effective measures to prevent any large-scale risk transmission in the long run.
基金partially supported by NIH grants (2U19AI090023,5P30AI50409,and R01GM122083)
文摘Study of gene expression has been arguably the most active research field in functional genomics.Over the last two decades,various high-throughput technologies,from gene expression microarray to RNA-seq,have been widely applied to the wholegenome profiling of gene expression.The commonality of these experiments is that they measure the gene expression levels of"bulk"sample,which pools a large number(often in the scale of millions)of cells,and thus the measurements reflect the average expression
基金Fund supported by the National Natural Science Foundation of China(81460598 and 81660644)the Natural Science Foundation of Jiangsu Province(BK20170267)Guangxi Special Fund for the First-Class Discipline Construction Project(05019038).
文摘Objective Alzheimer's disease(AD)is the most common cause of dementia.The pathophysiology of the disease mostly remains unearthed,thereby challenging drug development for AD.This study aims to screen high throughput gene expression data using weighted co-expression network analysis(WGCNA)to explore the potential therapeutic targets.Methods The dataset of GSE36980 was obtained from the Gene Expression Omnibus(GEO)database.Normalization,quality control,filtration,and soft-threshold calculation were carried out before clustering the co-expressed genes into different modules.Furthermore,the correlation coefiidents between the modules and clinical traits were computed to identify the key modules.Gene ontology and pathway enrichment analyses were performed on the key module genes.The STRING database was used to construct the protein-protein interaction(PPI)networks,which were further analyzed by Cytoscape app(MCODE).Finally,validation of hub genes was conducted by external GEO datasets of GSE 1297 and GSE 28146.Results Co-expressed genes were clustered into 27 modules,among which 6 modules were identified as the key module relating to AD occurrence.These key modules are primarily involved in chemical synaptic transmission(G0:0007268),the tricarboxylic acid(TCA)cycle and respiratory electron transport(R-HSA-1428517).WDR47,OXCT1,C3orfl4,ATP6V1A,SLC25A14,NAPB were found as the hub genes and their expression were validated by external datasets.Conclusions Through modules co-expression network analyses and PPI network analyses,we identified the hub genes of AD,including WDR47,0XCT1,C3orfl4i ATP6V1A,SLC25A14 and NAPB.Among them,three hub genes(ATP6V1A,SLC25A14,OXCT1)might contribute to AD pathogenesis through pathway of TCA cycle.
文摘The tool system of the organizational risk analyzer (ORA) to study the network of East Turkistan terrorists is selected. The model of the relationships among its personnel, knowledge, resources and task entities is represented by the meta-matrix in ORA, with which to analyze the risks and vulnerabilities of organizational structure quantitatively, and obtain the last vulnerabilities and risks of the organization. Case study in this system shows that it should be a shortcut to destroy effectively the network of terrorists by recognizing the caucus persons of the terrorism organization for the first and eliminating them when strikes the terror organization. It is vital to ensure effective use of the resources and control the risks of terrorist attacks.
