[ Objective] To observe the disposition and localization of neturopetide Y-Y1 （ NPY-Y1 ） mRNA in hypothalamus, pituitary and ovary during puberty. [ Methed] Three 60-day-old Sujiang sows weighing 20 kg and three 160...[ Objective] To observe the disposition and localization of neturopetide Y-Y1 （ NPY-Y1 ） mRNA in hypothalamus, pituitary and ovary during puberty. [ Methed] Three 60-day-old Sujiang sows weighing 20 kg and three 160-day-old Sujiang sows weighing 80 kg were selected and anaesthetized. The hypothalamus, pituitary and ovary were taken out for preparation of frozen sections. The expression and localization of the NPY-Y1 mRNA in hypothalamus-pituitary-ovary axis was observed by the in situ hybridization. The PBS was substituted for hybridization solution to set up a control. ~ Result] Positive hybridization signals of the NPY-Y1 mRNA were detected in the hypothalamus, pituitary and ovary at different develop- mental stages, and these signals were stronger in the 60-day-old sows （before the puberty） than in the 160-day-old sows （during the puberty）. E Conclusion] The NPY-Y1 mRNA is distributed in the hypothalamus-pituitary-ovary axis and can regulate reproductive process of sows.展开更多
文摘[ Objective] To observe the disposition and localization of neturopetide Y-Y1 （ NPY-Y1 ） mRNA in hypothalamus, pituitary and ovary during puberty. [ Methed] Three 60-day-old Sujiang sows weighing 20 kg and three 160-day-old Sujiang sows weighing 80 kg were selected and anaesthetized. The hypothalamus, pituitary and ovary were taken out for preparation of frozen sections. The expression and localization of the NPY-Y1 mRNA in hypothalamus-pituitary-ovary axis was observed by the in situ hybridization. The PBS was substituted for hybridization solution to set up a control. ~ Result] Positive hybridization signals of the NPY-Y1 mRNA were detected in the hypothalamus, pituitary and ovary at different develop- mental stages, and these signals were stronger in the 60-day-old sows （before the puberty） than in the 160-day-old sows （during the puberty）. E Conclusion] The NPY-Y1 mRNA is distributed in the hypothalamus-pituitary-ovary axis and can regulate reproductive process of sows.
