Using predatory mites Neoseiulus cucumeris Oudermans dusted with en- tomopathgen Paecilomyces fumosoroseus （Wize） Brown ＆ Smith to control aphids and spider mites is a new idea of biological control. Some studies w...Using predatory mites Neoseiulus cucumeris Oudermans dusted with en- tomopathgen Paecilomyces fumosoroseus （Wize） Brown ＆ Smith to control aphids and spider mites is a new idea of biological control. Some studies were conducted to confirm the feasibility of this idea. The germination condition of P. fumosoroseus conidia was determined by the method of different saturated salt solutions applying different humidity in small containers, and the pathogenicity test and the pot experi- ment were also conducted, under a suitable condition. There were 6 treatments for pot experiment as following： （1） CK; （2） releasing 50 N. cucumeds, （3） spraying 20 ml of 10^7 spores/ml conidial suspension; （4） releasing 50 N. cucumeris after spraying 20 ml of 10^7 spores/ml conidial suspension; （5） releasing 20 N. cucumeris dusted conidia of P. fumosoroseus; and （6） releasing 50 N. cucumeris dusted with conidia of P. fumosoroseus. The result showed that the germination condition of P. fu- mosoroseus conidia was 25-30 ℃ and 95%-100% RH, and under this condition, the 10s spores/ml conidia suspension caused a mortality of 86.52% in aphids and 41.78% in predatory mites after ten days. From the pot experiment, the numbers of aphids of treatment （3）, （4）, （6） were close to 0 at the 8th day. This study proved that the predatory mites N. cucumeris dusted with the entomogenous P. fu- mosoroseus could provide satisfactory control on both aphids and spider mites in potted eggplants under suitable temperature and humidity. The predatory mite vec- toring entomopathogen system could provide an economic and labor saving way for aphid control.展开更多
The entomopathogenic fungus Beauveria bassiana （Balsamo） Vuillemin and predatory mite Neoseiulus barked Hughes are effective biological control agents of Frankliniella occidentalis Pergande （Thysanoptera： Thripida...The entomopathogenic fungus Beauveria bassiana （Balsamo） Vuillemin and predatory mite Neoseiulus barked Hughes are effective biological control agents of Frankliniella occidentalis Pergande （Thysanoptera： Thripidae）, one of the most important pests of ornamentals and vegetables world-wide. Combined application of both may enhance control efficiency. The functional response for N. barked on the first instar larvae of western flower thrips which were infected by B. bassiana for 12 and 24 h in the laboratory （（25＋1）℃, （70＋5）% RH, L：D=16 h：8 h） was determined. The virulence of B. bassiana against the second instar and pupae of the thrips attacked by N. barked were also tested. The results showed that N. barked exhibited a Holling type II functional response on treated thrips. After having been treated with the fungus for 12 h and then offered to the predator, thrips were more vulnerable to be killed by N. barked. The second instar larvae and pupae of the thrips which had been attacked by predatory mites were markedly more susceptible to B. bassiana infection than normal thrips; the cumulative corrected mortality of B. bassiana of the second instar and pupae which were attacked by N. barked were 57 and 94%, respectively, compared to 35 and 80% in controls on the day 8. These findings highlight the potential use of B. bassiana in combination with N. barkeri to control F. occidentalis.展开更多
To find the method for the integrated control of Tetranychus cinnabarinus （carmine spider mite） and delay its resistance to pesticides, the joint actions of each of five commonly pesticides （abamectin, azadirachtin...To find the method for the integrated control of Tetranychus cinnabarinus （carmine spider mite） and delay its resistance to pesticides, the joint actions of each of five commonly pesticides （abamectin, azadirachtin, matrine, pyrethrins and imidacloprid）, and Neoseiulus cucumeris （Oudemans）, the natural enemy of cotton red spider mite, were studied. N. cucumeris was released after application of pesticides for seven days （for 0,3% azadirachtin EC） or six days （for other pesticides）. The results showed that the combined action of 1.8% abamectin EW （1：8 000） and N. cucumeris had the best control efficacy of 96.63% at 20 days after N. cucumeris releasing. The control efficacies of N. cucumeds and 0.3% azadirachtin EC （1：250） were 59.7% and 90.1% after one day and 20 days, respectively, after N. cucumeris releasing. The control efficacy of N. cucumeris and 0.5% matrine AS （1：2 000） was 82.65% at 20 days after N. cucumeris releasing. The results provide options for sustainable control of T. cinnabarinus and for the delay of pesticides resistance.展开更多
Neoseiulus barker/Hughes is one of the best biological control products. The paper reviewed taxonomic status, biological characteristics, large-scale breeding and application research status of N. barkeri, so as to pr...Neoseiulus barker/Hughes is one of the best biological control products. The paper reviewed taxonomic status, biological characteristics, large-scale breeding and application research status of N. barkeri, so as to provide scientific basis for further research and application of N. barkeri.展开更多
The effect of seven constant temperatures of 15, 20, 25, 27, 30, 35 and 37℃ on developmental time ofNeoseiulus barkeri Hughes were determined in laboratory conditions under 65% - 5% RH and a photoperiod of 12 ： 12 ...The effect of seven constant temperatures of 15, 20, 25, 27, 30, 35 and 37℃ on developmental time ofNeoseiulus barkeri Hughes were determined in laboratory conditions under 65% - 5% RH and a photoperiod of 12 ： 12 （L ： D） h on nymphal stages of Tetranychus urticae Koch. Total developmental time of females （from egg to adult emergence） at the above-mentioned temperatures was 26.59, 14.43, 6.32, 5.64, 4.59, 3.98 and 4.67 days, respectively. Developmental rate of the N. barkeri increased as temperature increased from 15 to 35℃, but declined at 37℃. A linear and two nonlinear models were fitted to developmental rate of immature stages ofN. barkeri to predict the developmental rate as a function of temperature, as well as to estimate the thermal constant （K） and critical temperatures （i.e., Tmin, Topt and Tmax）. The estimated values of the Tmin and K for total developmental time using the linear model were 12.07℃ and 86.20 degree-days （DD）, respectively. The Trnin and Tmax estimated by the Sharpe-Schoolfield-Ikemoto （SSI） model were 11.90℃ and 37.41℃, respectively. The estimated Topt for overall immature stage development ofN. barkeri by the Lactin and SSI models were 33.89℃ and 24.51℃, respectively. Based on the biological criteria of model evaluation, the linear and SSI models were found to be the best models for describing the developmental rate of overall immature stages of N. barkeri and estimating the temperature thresholds.展开更多
This study was carried out on the ability of predatory thrips Scolothrips longi- cornis Priesner to feed on 2 phytoseiid species and vice versa. Also the effect of predation of Neoseiulus californicus （McGregor） on ...This study was carried out on the ability of predatory thrips Scolothrips longi- cornis Priesner to feed on 2 phytoseiid species and vice versa. Also the effect of predation of Neoseiulus californicus （McGregor） on Typhlodromus bagdasarjani Wainstein and Aru- tunjan and vice versa was evaluated. The larvae, prepupae, and pupae of thrips and the eggs, larvae, and protonymphs of phytoseiids were selected as intraguild prey. The in- traguild predation （IGP） among S. longicornis and 2 phytoseiid species was unidirectional and in favor of phytoseiids, i.e., S. longicornis was not able to feed on larval stages of 2 phytoseiids. However, N. californicus and T. bagdasarjani fed on the 1st instar larvae （1.39 and 0.80 per day）, 2nd instar larvae （0.87 and 0.55 per day）, prepupae （0.51 and 0.48 per day）, and pupae of thrips （0.51 and 0.49 per day, respectively）. Both phytoseiids fed on eggs, larvae, and protonymphal stages of each other. Females of N. californicus con- sumed more phytoseiid larvae （2.49 per day） than T. bagdasarjani, which consumed 1.08 N. californicus larvae per day. When Tetranychus urticae was presented as an extraguild prey, intensity oflGP between 2 species ofphytoseiids and on larval stages ofS. longicornis reduced significantly. Therefore, it is concluded that （i） IGP existed among the 3 examined species and lack of feeding of S. longicornis on 2 phytoseiid species can be justified by its feeding type （monophagy）, （ii） N. californicus was much more prone to IGP than was T. bagdasarjani.展开更多
This survey had the aim to identify the mite fauna and mite ecologic interaction in rice culture, harvest 2010-2011, in Taquari and Cachoeirinha Counties, state of Rio Grande do Sul, Brazil. The mite population was bi...This survey had the aim to identify the mite fauna and mite ecologic interaction in rice culture, harvest 2010-2011, in Taquari and Cachoeirinha Counties, state of Rio Grande do Sul, Brazil. The mite population was biweekly evaluated on IRGA 424 and INTA PUITA CL cultivars in four areas where randomly sampled 20 plants/area. Fluctuation, ecologic indices and correlation between species and environmental factors were calculated. A total of 1,626 mites belonging to 14 species from 12 families were collected. Of the total specimens collected, 34.56% was on IRGA 424, in Taquari, 32.47% on IRGA 424-120 and 28.35% on IRGA 424-60, in Cachoeirinha and 4.61% on 1NTA PUIT,g, CL, in Taquari. Family Ascidae showed great richness, with four species, Lasioseius sp., Lasioseius sp., Proctolaelaps sp. and Cheiroseius sp.. Schizotetranychus oryzae Rossi de Simons （86.65%） was the phytophagous mite more abundant, while among the predators Neoseiulus paraibensis （Moraes and McMurtry） （6.88%） stood out. The population peaks of S. oryzae and N. paraibensis happened on March 2011. In all evaluated areas, the correlation between S. oryzae and N. paraibensis was positive, specially on IRGA 424-120 （r = 0.93, P = 0.006）.展开更多
基金Supported by Youth Science Fund Project of Fujian Academy of Agricultural Sciences(2012DQA-2)Innovation Team Project of Fujian Academy of Agricultural Sciences(CXTD-2-1313)+1 种基金Natural Science Fund of Fujian Province(2014J1108)National 863Program(2011AA10A201)~~
文摘Using predatory mites Neoseiulus cucumeris Oudermans dusted with en- tomopathgen Paecilomyces fumosoroseus （Wize） Brown ＆ Smith to control aphids and spider mites is a new idea of biological control. Some studies were conducted to confirm the feasibility of this idea. The germination condition of P. fumosoroseus conidia was determined by the method of different saturated salt solutions applying different humidity in small containers, and the pathogenicity test and the pot experi- ment were also conducted, under a suitable condition. There were 6 treatments for pot experiment as following： （1） CK; （2） releasing 50 N. cucumeds, （3） spraying 20 ml of 10^7 spores/ml conidial suspension; （4） releasing 50 N. cucumeris after spraying 20 ml of 10^7 spores/ml conidial suspension; （5） releasing 20 N. cucumeris dusted conidia of P. fumosoroseus; and （6） releasing 50 N. cucumeris dusted with conidia of P. fumosoroseus. The result showed that the germination condition of P. fu- mosoroseus conidia was 25-30 ℃ and 95%-100% RH, and under this condition, the 10s spores/ml conidia suspension caused a mortality of 86.52% in aphids and 41.78% in predatory mites after ten days. From the pot experiment, the numbers of aphids of treatment （3）, （4）, （6） were close to 0 at the 8th day. This study proved that the predatory mites N. cucumeris dusted with the entomogenous P. fu- mosoroseus could provide satisfactory control on both aphids and spider mites in potted eggplants under suitable temperature and humidity. The predatory mite vec- toring entomopathogen system could provide an economic and labor saving way for aphid control.
基金supported by the Special Fund for AgroScientific Research in the Public Interest, China (200903032)National Modern Agricultural Science and Technology City Industry of Beijing, China (Z121100001212006)
文摘The entomopathogenic fungus Beauveria bassiana （Balsamo） Vuillemin and predatory mite Neoseiulus barked Hughes are effective biological control agents of Frankliniella occidentalis Pergande （Thysanoptera： Thripidae）, one of the most important pests of ornamentals and vegetables world-wide. Combined application of both may enhance control efficiency. The functional response for N. barked on the first instar larvae of western flower thrips which were infected by B. bassiana for 12 and 24 h in the laboratory （（25＋1）℃, （70＋5）% RH, L：D=16 h：8 h） was determined. The virulence of B. bassiana against the second instar and pupae of the thrips attacked by N. barked were also tested. The results showed that N. barked exhibited a Holling type II functional response on treated thrips. After having been treated with the fungus for 12 h and then offered to the predator, thrips were more vulnerable to be killed by N. barked. The second instar larvae and pupae of the thrips which had been attacked by predatory mites were markedly more susceptible to B. bassiana infection than normal thrips; the cumulative corrected mortality of B. bassiana of the second instar and pupae which were attacked by N. barked were 57 and 94%, respectively, compared to 35 and 80% in controls on the day 8. These findings highlight the potential use of B. bassiana in combination with N. barkeri to control F. occidentalis.
基金Supported by the Modern Agricultural Technology System of Hunan Province(Xiangnongyelian No.[2012]278)the Project of the Department of Science and Technology of Hunan Province(2010NK3011,2011FJ4298)~~
文摘To find the method for the integrated control of Tetranychus cinnabarinus （carmine spider mite） and delay its resistance to pesticides, the joint actions of each of five commonly pesticides （abamectin, azadirachtin, matrine, pyrethrins and imidacloprid）, and Neoseiulus cucumeris （Oudemans）, the natural enemy of cotton red spider mite, were studied. N. cucumeris was released after application of pesticides for seven days （for 0,3% azadirachtin EC） or six days （for other pesticides）. The results showed that the combined action of 1.8% abamectin EW （1：8 000） and N. cucumeris had the best control efficacy of 96.63% at 20 days after N. cucumeris releasing. The control efficacies of N. cucumeds and 0.3% azadirachtin EC （1：250） were 59.7% and 90.1% after one day and 20 days, respectively, after N. cucumeris releasing. The control efficacy of N. cucumeris and 0.5% matrine AS （1：2 000） was 82.65% at 20 days after N. cucumeris releasing. The results provide options for sustainable control of T. cinnabarinus and for the delay of pesticides resistance.
基金Supported by Scientific and Technological Project of Chongqing City(CSTC,2010AC1018)Development Plan for Changjiang Scholars and Innovative Team(IRT0976)+1 种基金Special Fund for Agro-scientific Research in the Public Interest(200903032)Open Fund Projects of Municipal Key Laboratory for Citrus in Chongqing City
文摘Neoseiulus barker/Hughes is one of the best biological control products. The paper reviewed taxonomic status, biological characteristics, large-scale breeding and application research status of N. barkeri, so as to provide scientific basis for further research and application of N. barkeri.
文摘The effect of seven constant temperatures of 15, 20, 25, 27, 30, 35 and 37℃ on developmental time ofNeoseiulus barkeri Hughes were determined in laboratory conditions under 65% - 5% RH and a photoperiod of 12 ： 12 （L ： D） h on nymphal stages of Tetranychus urticae Koch. Total developmental time of females （from egg to adult emergence） at the above-mentioned temperatures was 26.59, 14.43, 6.32, 5.64, 4.59, 3.98 and 4.67 days, respectively. Developmental rate of the N. barkeri increased as temperature increased from 15 to 35℃, but declined at 37℃. A linear and two nonlinear models were fitted to developmental rate of immature stages ofN. barkeri to predict the developmental rate as a function of temperature, as well as to estimate the thermal constant （K） and critical temperatures （i.e., Tmin, Topt and Tmax）. The estimated values of the Tmin and K for total developmental time using the linear model were 12.07℃ and 86.20 degree-days （DD）, respectively. The Trnin and Tmax estimated by the Sharpe-Schoolfield-Ikemoto （SSI） model were 11.90℃ and 37.41℃, respectively. The estimated Topt for overall immature stage development ofN. barkeri by the Lactin and SSI models were 33.89℃ and 24.51℃, respectively. Based on the biological criteria of model evaluation, the linear and SSI models were found to be the best models for describing the developmental rate of overall immature stages of N. barkeri and estimating the temperature thresholds.
文摘This study was carried out on the ability of predatory thrips Scolothrips longi- cornis Priesner to feed on 2 phytoseiid species and vice versa. Also the effect of predation of Neoseiulus californicus （McGregor） on Typhlodromus bagdasarjani Wainstein and Aru- tunjan and vice versa was evaluated. The larvae, prepupae, and pupae of thrips and the eggs, larvae, and protonymphs of phytoseiids were selected as intraguild prey. The in- traguild predation （IGP） among S. longicornis and 2 phytoseiid species was unidirectional and in favor of phytoseiids, i.e., S. longicornis was not able to feed on larval stages of 2 phytoseiids. However, N. californicus and T. bagdasarjani fed on the 1st instar larvae （1.39 and 0.80 per day）, 2nd instar larvae （0.87 and 0.55 per day）, prepupae （0.51 and 0.48 per day）, and pupae of thrips （0.51 and 0.49 per day, respectively）. Both phytoseiids fed on eggs, larvae, and protonymphal stages of each other. Females of N. californicus con- sumed more phytoseiid larvae （2.49 per day） than T. bagdasarjani, which consumed 1.08 N. californicus larvae per day. When Tetranychus urticae was presented as an extraguild prey, intensity oflGP between 2 species ofphytoseiids and on larval stages ofS. longicornis reduced significantly. Therefore, it is concluded that （i） IGP existed among the 3 examined species and lack of feeding of S. longicornis on 2 phytoseiid species can be justified by its feeding type （monophagy）, （ii） N. californicus was much more prone to IGP than was T. bagdasarjani.
文摘This survey had the aim to identify the mite fauna and mite ecologic interaction in rice culture, harvest 2010-2011, in Taquari and Cachoeirinha Counties, state of Rio Grande do Sul, Brazil. The mite population was biweekly evaluated on IRGA 424 and INTA PUITA CL cultivars in four areas where randomly sampled 20 plants/area. Fluctuation, ecologic indices and correlation between species and environmental factors were calculated. A total of 1,626 mites belonging to 14 species from 12 families were collected. Of the total specimens collected, 34.56% was on IRGA 424, in Taquari, 32.47% on IRGA 424-120 and 28.35% on IRGA 424-60, in Cachoeirinha and 4.61% on 1NTA PUIT,g, CL, in Taquari. Family Ascidae showed great richness, with four species, Lasioseius sp., Lasioseius sp., Proctolaelaps sp. and Cheiroseius sp.. Schizotetranychus oryzae Rossi de Simons （86.65%） was the phytophagous mite more abundant, while among the predators Neoseiulus paraibensis （Moraes and McMurtry） （6.88%） stood out. The population peaks of S. oryzae and N. paraibensis happened on March 2011. In all evaluated areas, the correlation between S. oryzae and N. paraibensis was positive, specially on IRGA 424-120 （r = 0.93, P = 0.006）.
