The economic alga Neopyropia,which holds significant value in China for its edibility and economic potential,harbors diverse epiphytic bacteria on its surface.The epiphytic bacteria on Neopyropia yezoensis and Neopyro...The economic alga Neopyropia,which holds significant value in China for its edibility and economic potential,harbors diverse epiphytic bacteria on its surface.The epiphytic bacteria on Neopyropia yezoensis and Neopyropia katadae,two nori species of Neopyropia living at the same tidal level but with distinct physiological states and living habits,were investigated to understand the differences between them and the effects of epiphytic bacteria on their differential characteristics.Analysis of 16S rRNA gene sequences and real-time quantitative PCR(qPCR)of thalli and seawater samples(n=24)revealed notable differences in microbial diversity and community structure between the two species.Bacteroidetes dominated the bacterial communities in association with N.yezoensis,whereas Proteobacteria were predominant in N.katadae.Phylogenetic Investigation of Communities by Reconstruction of Unobserved States(PICRUSt2)analysis revealed enriched genes that related to the ABC transport system,iron complex outer membrane receptor proteins,and proliferation in N.yezoensis,whereas N.katadae exhibited enrichment of genes that related to energy supply as well as cell wall and cell membrane production.The results of qPCR indicate a higher abundance of epiphytic bacteria on surface of N.yezoensis than those on surface of N.katadae.The findings indicate that differences in the living environments of N.yezoensis and N.katadae may result in distinct structural composition and abundance of epiphytic bacteria on their surfaces,thereby conferring specific biological functionalities to each microbial community,and the epiphytic bacteria may shape the living habits of hosts to some extents.This study provided a basis for understanding the close association between surface microorganisms and algal bodies,and the physiological and ecological characteristics of nori species.展开更多
[Objectives]To study the differences of growth rate,morphology,ultrastructure,pigment content and antioxidant enzyme activity of free-living conchocelis of cultivated type of Neopyropia yezoensis under different light...[Objectives]To study the differences of growth rate,morphology,ultrastructure,pigment content and antioxidant enzyme activity of free-living conchocelis of cultivated type of Neopyropia yezoensis under different light qualities(white,red,blue,and green light).[Methods]The study was carried out through light quality design and culture,growth rate determination,microstructure and ultrastructure observation,chlorophyll a content and carotenoid content determination,phycobiliprotein content determination,malondialdehyde(MDA)content determination,superoxide dismutase(SOD)activity determination.[Results]After 21 d of culture,the specific growth rate(SGR)and chlorophyll a content of free-living conchocelis of N.yezoensis were significantly increased by white light(WL),followed by red light(RL)and green light(GL),and they were the lowest under blue light(BL).Compared with the WL group,the BL group had the highest content of phycoerythrin(PE),and the RL and GL groups had the highest content of phycocyanin(PC).The algal body of WL group was normal black brown,and the cell wall was the thickest.In RL and GL groups,the algal bodies were green,and their diameters and cell wall thicknesses were similar to those in WL group.In BL group,the algal body was bright red,the diameter was the smallest,the cell wall was the thinnest,and the ultrastructure showed that the number of plastoglobulus on the thylakoid was the largest.After BL irradiation,the highest MDA content and the lowest SOD activity were observed.The results revealed that WL is the most beneficial to the growth of free-living conchocelis,followed by RL and GL,while BL has adverse effects.[Conclusions]This study explored the most suitable light quality conditions for the propagation of free-living conchocelis.It is expected to provide germplasm guarantee for the production and seedling of N.yezoensis.展开更多
The archeospores released from the blades of Neopyropia yezoensis via dedifferentiated vegetative cells have drawn attention both in cultivation and research.Wounding-induced archeospores formation and release have be...The archeospores released from the blades of Neopyropia yezoensis via dedifferentiated vegetative cells have drawn attention both in cultivation and research.Wounding-induced archeospores formation and release have been observed in N.yezoensis,but the mechanism behind them is unclear.In this study,the involvement of reactive oxygen species(ROS)in the process of wounding-induced archeospores formation and release was investigated.Based on ROS fluorescence observations,the blade fragments began to accumulate ROS after 12 h wounding,while no ROS signals were observed in normal blades.Next,when the blade fragments were treated with ROS inhibitor diphenyleneiodonium(DPI),it showed that the production of archeopores was significantly suppressed.Under normal culturing after wounding,78.3%of the fragments could release archeospores,and the ROS fluorescence was enriched in the released archeospores.Under 0.05μM DPI treatment,the percentage of fragments releasing archeospores was dropped to 16.2%,with decreased ROS fluorescence levels.Under 0.1μM DPI treatment,no archeospores were released from the fragments,and ROS fluorescence was also undetectable in the fragments.Our findings proved that ROS are essential for wounding-induced archeospores production,which might play regulatory roles in the cell dedifferentiation of N.yezoensis.展开更多
为解决条斑紫菜(Neopyropia yezoensis)遗传育种研究中缺乏生长性状遗传位点的问题,本研究针对条斑紫菜配子体的4个重要生长性状展开了全基因组关联分析(Genome-wide association study,GWAS)。首先,在相同环境下纯化培养了130个单一基...为解决条斑紫菜(Neopyropia yezoensis)遗传育种研究中缺乏生长性状遗传位点的问题,本研究针对条斑紫菜配子体的4个重要生长性状展开了全基因组关联分析(Genome-wide association study,GWAS)。首先,在相同环境下纯化培养了130个单一基因型条斑紫菜个体,并测量了叶长、叶宽、鲜质量和干质量这4个与生长相关的表型数据,进而分析并发现条斑紫菜不同生长性状的变异系数(Coefficient of variation)为32.63%~50.95%,各性状之间存在显著的相关性。利用全基因组重测序技术获得每个个体的基因型。对重测序数据质检后,共得到405999个高质量的单核苷酸多态性(Single nucleotide polymorphism,SNP)位点,其中53077个位点位于外显子区域,SNPs位点转换与颠换的数量之比为1.23。群体结构分析发现,研究群体可以分为4个亚群,不同亚群之间存在明显的遗传差异,而分群情况并非严格按照地理位置划分。测序个体之间的亲缘关系较远,连锁不平衡(Linkage disequilibrium,LD)衰减距离较小,这表明样本的遗传变异比较丰富。本研究利用混合线性模型(Q+K)进行了性状关联位点的筛选,共筛选出90个显著关联位点,其中67个位点与叶长显著相关,17个位点与叶宽显著相关,4个位点与鲜质量显著相关,2个位点与干质量显著相关,这些位点能够解释16.12%~24.85%的表型变异。在显著性位点上、下游20 kb范围内筛选,得到39个可能与生长性状相关的候选基因,基因功能涉及细胞增殖、控制细胞代谢途径以及调控植物的生长发育等。本文为条斑紫菜分子育种研究的开展提供了候选遗传位点和重要依据。展开更多
基金Supported by the National Natural Science Foundation of China(Nos.42276148,41976133)。
文摘The economic alga Neopyropia,which holds significant value in China for its edibility and economic potential,harbors diverse epiphytic bacteria on its surface.The epiphytic bacteria on Neopyropia yezoensis and Neopyropia katadae,two nori species of Neopyropia living at the same tidal level but with distinct physiological states and living habits,were investigated to understand the differences between them and the effects of epiphytic bacteria on their differential characteristics.Analysis of 16S rRNA gene sequences and real-time quantitative PCR(qPCR)of thalli and seawater samples(n=24)revealed notable differences in microbial diversity and community structure between the two species.Bacteroidetes dominated the bacterial communities in association with N.yezoensis,whereas Proteobacteria were predominant in N.katadae.Phylogenetic Investigation of Communities by Reconstruction of Unobserved States(PICRUSt2)analysis revealed enriched genes that related to the ABC transport system,iron complex outer membrane receptor proteins,and proliferation in N.yezoensis,whereas N.katadae exhibited enrichment of genes that related to energy supply as well as cell wall and cell membrane production.The results of qPCR indicate a higher abundance of epiphytic bacteria on surface of N.yezoensis than those on surface of N.katadae.The findings indicate that differences in the living environments of N.yezoensis and N.katadae may result in distinct structural composition and abundance of epiphytic bacteria on their surfaces,thereby conferring specific biological functionalities to each microbial community,and the epiphytic bacteria may shape the living habits of hosts to some extents.This study provided a basis for understanding the close association between surface microorganisms and algal bodies,and the physiological and ecological characteristics of nori species.
基金Supported by National Algae System(CARS-50)Modern Agricultural(Laver)Industrial Technology System of Jiangsu Province(JATS[2023]381)Research Project of Nantong City(MS22022065).
文摘[Objectives]To study the differences of growth rate,morphology,ultrastructure,pigment content and antioxidant enzyme activity of free-living conchocelis of cultivated type of Neopyropia yezoensis under different light qualities(white,red,blue,and green light).[Methods]The study was carried out through light quality design and culture,growth rate determination,microstructure and ultrastructure observation,chlorophyll a content and carotenoid content determination,phycobiliprotein content determination,malondialdehyde(MDA)content determination,superoxide dismutase(SOD)activity determination.[Results]After 21 d of culture,the specific growth rate(SGR)and chlorophyll a content of free-living conchocelis of N.yezoensis were significantly increased by white light(WL),followed by red light(RL)and green light(GL),and they were the lowest under blue light(BL).Compared with the WL group,the BL group had the highest content of phycoerythrin(PE),and the RL and GL groups had the highest content of phycocyanin(PC).The algal body of WL group was normal black brown,and the cell wall was the thickest.In RL and GL groups,the algal bodies were green,and their diameters and cell wall thicknesses were similar to those in WL group.In BL group,the algal body was bright red,the diameter was the smallest,the cell wall was the thinnest,and the ultrastructure showed that the number of plastoglobulus on the thylakoid was the largest.After BL irradiation,the highest MDA content and the lowest SOD activity were observed.The results revealed that WL is the most beneficial to the growth of free-living conchocelis,followed by RL and GL,while BL has adverse effects.[Conclusions]This study explored the most suitable light quality conditions for the propagation of free-living conchocelis.It is expected to provide germplasm guarantee for the production and seedling of N.yezoensis.
基金the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2022fjscq01)the National Key Research and Development Program of China for financial support(2018YFD0900606).
文摘The archeospores released from the blades of Neopyropia yezoensis via dedifferentiated vegetative cells have drawn attention both in cultivation and research.Wounding-induced archeospores formation and release have been observed in N.yezoensis,but the mechanism behind them is unclear.In this study,the involvement of reactive oxygen species(ROS)in the process of wounding-induced archeospores formation and release was investigated.Based on ROS fluorescence observations,the blade fragments began to accumulate ROS after 12 h wounding,while no ROS signals were observed in normal blades.Next,when the blade fragments were treated with ROS inhibitor diphenyleneiodonium(DPI),it showed that the production of archeopores was significantly suppressed.Under normal culturing after wounding,78.3%of the fragments could release archeospores,and the ROS fluorescence was enriched in the released archeospores.Under 0.05μM DPI treatment,the percentage of fragments releasing archeospores was dropped to 16.2%,with decreased ROS fluorescence levels.Under 0.1μM DPI treatment,no archeospores were released from the fragments,and ROS fluorescence was also undetectable in the fragments.Our findings proved that ROS are essential for wounding-induced archeospores production,which might play regulatory roles in the cell dedifferentiation of N.yezoensis.
文摘为解决条斑紫菜(Neopyropia yezoensis)遗传育种研究中缺乏生长性状遗传位点的问题,本研究针对条斑紫菜配子体的4个重要生长性状展开了全基因组关联分析(Genome-wide association study,GWAS)。首先,在相同环境下纯化培养了130个单一基因型条斑紫菜个体,并测量了叶长、叶宽、鲜质量和干质量这4个与生长相关的表型数据,进而分析并发现条斑紫菜不同生长性状的变异系数(Coefficient of variation)为32.63%~50.95%,各性状之间存在显著的相关性。利用全基因组重测序技术获得每个个体的基因型。对重测序数据质检后,共得到405999个高质量的单核苷酸多态性(Single nucleotide polymorphism,SNP)位点,其中53077个位点位于外显子区域,SNPs位点转换与颠换的数量之比为1.23。群体结构分析发现,研究群体可以分为4个亚群,不同亚群之间存在明显的遗传差异,而分群情况并非严格按照地理位置划分。测序个体之间的亲缘关系较远,连锁不平衡(Linkage disequilibrium,LD)衰减距离较小,这表明样本的遗传变异比较丰富。本研究利用混合线性模型(Q+K)进行了性状关联位点的筛选,共筛选出90个显著关联位点,其中67个位点与叶长显著相关,17个位点与叶宽显著相关,4个位点与鲜质量显著相关,2个位点与干质量显著相关,这些位点能够解释16.12%~24.85%的表型变异。在显著性位点上、下游20 kb范围内筛选,得到39个可能与生长性状相关的候选基因,基因功能涉及细胞增殖、控制细胞代谢途径以及调控植物的生长发育等。本文为条斑紫菜分子育种研究的开展提供了候选遗传位点和重要依据。
