Two new triterpenoids, 3 β, 15α, 21 β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β , 6 α , 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydro...Two new triterpenoids, 3 β, 15α, 21 β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β , 6 α , 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydroxybenzoic acid (4), scosoletin (5), anthraquinones chrysophanol (6), 4H-1-benzopyran-4-one, 5, 7-dihydroxy-2-methyl (7),β-sitosterol (8) and stigmasterol glucoside (9) were isolated by the chromatography of the silica gel, RP-18 and Sephadex-LH 20 from the EtOAc extract of Neonauclea sessilifolia (Roxb.) Merr. ( Rubiaceae ). Their structures were elucidated based on spectral analysis including 1D-, 2D-NMR (HMQC, HMBC), IR and EIMS. Among them, compound 6 was shown to possess inhibitory activity on the growth of Mycobacterium tuberculosis (Zopf) Lehmann et Neumann with a minimum inhibitory amount of 25μg, compounds 2 and 4 also showed weak inhibitory activities on the growteof M. tuberculosis.展开更多
Neonauclea sessilifolia belongs to Rubiaceae and its chemical constituents and pharmacological investigations were seldom reported. To search the active constituents of the plant, the ethanol of the dried stem of Neon...Neonauclea sessilifolia belongs to Rubiaceae and its chemical constituents and pharmacological investigations were seldom reported. To search the active constituents of the plant, the ethanol of the dried stem of Neonauclea sessilifolia was separated via reflux with ethanol, then the crude product was extracted with ethyl acetate and n-butanol in sequence. The ethyl acetate extract was further isolated by silica gel and Sephadex LH-20 column chromatography. A new triterpene was obtained, and the structure was identified as 3β, 7β, 21β, 23-tetrahydroxyolean-12-ene-28-oic acid via 1D, 2D NMR techniques and HR-EI-MS analysis.展开更多
文摘Two new triterpenoids, 3 β, 15α, 21 β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β , 6 α , 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydroxybenzoic acid (4), scosoletin (5), anthraquinones chrysophanol (6), 4H-1-benzopyran-4-one, 5, 7-dihydroxy-2-methyl (7),β-sitosterol (8) and stigmasterol glucoside (9) were isolated by the chromatography of the silica gel, RP-18 and Sephadex-LH 20 from the EtOAc extract of Neonauclea sessilifolia (Roxb.) Merr. ( Rubiaceae ). Their structures were elucidated based on spectral analysis including 1D-, 2D-NMR (HMQC, HMBC), IR and EIMS. Among them, compound 6 was shown to possess inhibitory activity on the growth of Mycobacterium tuberculosis (Zopf) Lehmann et Neumann with a minimum inhibitory amount of 25μg, compounds 2 and 4 also showed weak inhibitory activities on the growteof M. tuberculosis.
文摘Neonauclea sessilifolia belongs to Rubiaceae and its chemical constituents and pharmacological investigations were seldom reported. To search the active constituents of the plant, the ethanol of the dried stem of Neonauclea sessilifolia was separated via reflux with ethanol, then the crude product was extracted with ethyl acetate and n-butanol in sequence. The ethyl acetate extract was further isolated by silica gel and Sephadex LH-20 column chromatography. A new triterpene was obtained, and the structure was identified as 3β, 7β, 21β, 23-tetrahydroxyolean-12-ene-28-oic acid via 1D, 2D NMR techniques and HR-EI-MS analysis.
