Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic ...Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic crop,dragon fruit is prone to serious plant-parasitic nematode infestation;however,whether it encounters continuous cropping obstacles remains unclear.Here,we studied plant-parasitic nematodes(Meloidogyne spp.and Tylenchorhynchus sp.)in the soil and roots,soil nematode communities,metabolic footprint,soil integrated fertility,and the yield of intensively planted dragon fruit under non-continuous cropping(Y1)and 3 years(Y3)and 5 years(Y5)of continuous cropping,to determine potential continuous-cropping obstacles and factors that affect the yield of this fruit.The largest numbers of plant-parasitic nematodes in the soil and roots were observed in Y5;the associated yield was reduced,and the dragon fruit was severely stressed.Further analysis of the composition,diversity,and ecological function indices of soil nematodes showed that the soil ecological environment deteriorated after 3 years of continuous cropping,with Y5 having the worst results.Similarly,the soil at Y5 had a significant inhibitory effect on the growth and reproduction of Caenorhabditis elegans.Mantel test analysis and a random forest model showed that soil available phosphorus,soil exchange calcium,and soil nematode abundance and diversity were related significantly to yield.Partial least squares path modeling revealed that soil fertility and soil nematode diversity directly impacts the yield of continuously cropped dragon fruit.In summary,continuous cropping obstacles occurred in Y5 of intensive dragon fruit cultivation,with soil nematode diversity and soil fertility determining the crop's yield.展开更多
Entomopathogenic nematodes(EPNs)represent a promising biological control strategy for managing insect pest populations,offering an environmentally sustainable alternative to conventional chemical pesticides.This revie...Entomopathogenic nematodes(EPNs)represent a promising biological control strategy for managing insect pest populations,offering an environmentally sustainable alternative to conventional chemical pesticides.This review examines the application of EPNs in forestry,highlighting their biological and ecological characteristics,mechanisms of action,and efficacy against key forest pests.By exploring various methods of EPN application,including soil injection,foliar spray,and trunk injection,practical challenges and potential solutions for effective implementation are assessed.Case studies demonstrate successful use of EPNs in controlling pests such as bark beetles,wood borers,and root weevils,underscoring their potential for integration into integrated pest management(IPM)programs.Despite current limitations,including environmental sensitivity and application constraints,ongoing research and technological advancements continue to enhance the efficacy and reliability of EPNs.This review underscores the importance of EPNs in sustainable forestry practices and calls for further research to optimize their use and to address existing challenges,ultimately contributing to healthier forest ecosystems and reduced reliance on chemical pesticides.展开更多
Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomat...Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomatoes,Mi-1.2,fails at soil temperatures above 28℃.We cloned the heat-stable RKN-resistant gene,Mi-9,from a gene cluster composed of seven nucleotide-binding sites and leucine-rich repeat(NBS-LRR)type resistant genes in Solanum arcunum accession LA2157.Screening nematode infections in individual and combinatorial knockouts of five NBS-LRR genes showed that Mi-9 Candidate 4(MiC-4)alone is sufficient to confer heat-stable RKN resistance.Our study identifies a new source of heat-stable resistance to RKN in tomatoes for challenging environmental conditions.We also showcase a roadmap for rapid characterization of resistance genes by combining comparative genomics and genome editing,with the potential to be utilized in other crops.展开更多
Exotic plant invasions and increased atmospheric carbon dioxide(CO_(2))concentration have been determined to independently affect soil nematodes,a key component of soil biota.However,little is known about the long-ter...Exotic plant invasions and increased atmospheric carbon dioxide(CO_(2))concentration have been determined to independently affect soil nematodes,a key component of soil biota.However,little is known about the long-term effects of these two global change factors and their interactive effects.Over three consecutive years,we cultivated invasive alien plant Xanthium strumarium and its two phylogenetically related natives under both ambient(aCO_(2))and elevated(eCO_(2))atmospheric CO_(2)concentrations,and determined the effects of the invader and natives on soil nematodes under different CO_(2)concentrations and the relevant mechanism.The abundance of total soil nematodes and that of the dominant trophic group(herbivores)were significantly affected by plant species and CO_(2)concentration,and these effects were dependent on the experimental duration,however,the Shannon-diversity of nematodes was not affected by these factors.Under aCO_(2),both invasive and native species significantly increased the total nematode abundance and that of the dominant trophic group with increasing experimental duration,and the amplitude of the increase was greater under the invader relative to the natives.The eCO_(2)increased total nematode abundance(second year)and that of the dominant trophic group(third year)under the invader,but not under the natives(or even decreased)with increasing experimental duration.Root litter had greater effects on soil nematode abundance than leaf litter and root exudates did.This study indicates that eCO_(2)would aggravate effects of invasive plants on soil nematodes by increasing abundance,and these effects would vary with the duration.展开更多
Plant-parasitic nematodes(PPNs)(Meloidogyne sp.,Globodera sp.,and Pratylenchus sp.)and fungi are two of the most economically important groups of organisms affecting agricultural productivity worldwide.The interaction...Plant-parasitic nematodes(PPNs)(Meloidogyne sp.,Globodera sp.,and Pratylenchus sp.)and fungi are two of the most economically important groups of organisms affecting agricultural productivity worldwide.The interactions among PPNs,biocontrol fungi,and soil ecosystem can significantly impact plant health,disease management,and ecosystem functioning.We aimed to provide a comprehensive overview of the complex relationships between PPNs and biocontrol fungi,including pathogenic and biocontrol interactions.We summarized the molecular and ecological mechanisms underlying these interactions,highlighting the key players,signaling pathways,and environmental factors that influence the interactions.We also reviewed current knowledge on fungus-based biocontrol strategies against PPNs,including the development of novel management approaches.Furthermore,we explored the prospects of nematode-fungus interactions in agriculture,including the potential applications and technologies,precision agriculture,and integrated pest management approaches.This review highlights the need for further research on nematode-fungus interactions and their impact on plant infection and productivity,with an emphasis on the development of sustainable and effective strategies for managing PPNs and enhancing plant health in agricultural ecosystems.展开更多
The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population be...The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population belonging to the genus Aphelenchoides was isolated from symptomatic leaves of fuchsia plants(Fuchsia×hybrida Hort.ex Sieb.& Voss.) in Chengdu,Sichuan Province,China.Morphological and morphometric characteristics were determined using light microscopy and scanning electron microscopy.Detailed examination revealed diagnostic features consistent with A.fragariae.Three ribosomal DNA(rDNA) regions,i.e.,partial small subunit(SSU) rRNA,D2-D3 expansion segments of the large subunit(LSU) rRNA,and the internal transcribed spacer(ITS),were amplified and sequenced.Bayesian phylogenetic analyses based on these sequences placed the isolate in a well-supported monophyletic clade with reference A.fragariae specimens,clearly separated from other Aphelenchoides species.Furthermore,host-suitability assays demonstrated that this nematode population not only infects and reproduces on Fuchsia×hybrida,but also on Fragaria ananassa and Pteris vittata,two known hosts of A.fragariae.Collectively,morphological,molecular,and host-range evidence confirm the identification of this nematode as A.fragariae.To our knowledge,this represents the first molecular and morphological confirmation of A.fragariae in China,and the first report of Fuchsia×hybrida as a natural host for this species.展开更多
This paper summarized the history and present condition of studying and utilizing entomopathogenic nematodes at home and abroad, expounded its taxonomy, life cycle and the mechanism with symbiotic bacteria killing hos...This paper summarized the history and present condition of studying and utilizing entomopathogenic nematodes at home and abroad, expounded its taxonomy, life cycle and the mechanism with symbiotic bacteria killing host insect. Taxonomy, mycelial form, pathogenic function and anti-bacteria function of symbiotic bacteria were introduced. Production and utilization of entomopathogenic nematodes, the characteristic genetic improvement by use of biological engineering technology, as well as the existing problem and applying foreground were also discussed.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD1700203and 2022YFD1901501)the Tianchi Talent Introduction Program of Xinjiang Autonomous Region,China(2023-“2+5”)the Tingzhou Talent Introduction Program of Changji Autonomous Region,China(2023)。
文摘Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic crop,dragon fruit is prone to serious plant-parasitic nematode infestation;however,whether it encounters continuous cropping obstacles remains unclear.Here,we studied plant-parasitic nematodes(Meloidogyne spp.and Tylenchorhynchus sp.)in the soil and roots,soil nematode communities,metabolic footprint,soil integrated fertility,and the yield of intensively planted dragon fruit under non-continuous cropping(Y1)and 3 years(Y3)and 5 years(Y5)of continuous cropping,to determine potential continuous-cropping obstacles and factors that affect the yield of this fruit.The largest numbers of plant-parasitic nematodes in the soil and roots were observed in Y5;the associated yield was reduced,and the dragon fruit was severely stressed.Further analysis of the composition,diversity,and ecological function indices of soil nematodes showed that the soil ecological environment deteriorated after 3 years of continuous cropping,with Y5 having the worst results.Similarly,the soil at Y5 had a significant inhibitory effect on the growth and reproduction of Caenorhabditis elegans.Mantel test analysis and a random forest model showed that soil available phosphorus,soil exchange calcium,and soil nematode abundance and diversity were related significantly to yield.Partial least squares path modeling revealed that soil fertility and soil nematode diversity directly impacts the yield of continuously cropped dragon fruit.In summary,continuous cropping obstacles occurred in Y5 of intensive dragon fruit cultivation,with soil nematode diversity and soil fertility determining the crop's yield.
文摘Entomopathogenic nematodes(EPNs)represent a promising biological control strategy for managing insect pest populations,offering an environmentally sustainable alternative to conventional chemical pesticides.This review examines the application of EPNs in forestry,highlighting their biological and ecological characteristics,mechanisms of action,and efficacy against key forest pests.By exploring various methods of EPN application,including soil injection,foliar spray,and trunk injection,practical challenges and potential solutions for effective implementation are assessed.Case studies demonstrate successful use of EPNs in controlling pests such as bark beetles,wood borers,and root weevils,underscoring their potential for integration into integrated pest management(IPM)programs.Despite current limitations,including environmental sensitivity and application constraints,ongoing research and technological advancements continue to enhance the efficacy and reliability of EPNs.This review underscores the importance of EPNs in sustainable forestry practices and calls for further research to optimize their use and to address existing challenges,ultimately contributing to healthier forest ecosystems and reduced reliance on chemical pesticides.
基金supported by the National Key R&D Program of China(2018YFA0900600 and 2021YFF1000103-5)the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030503)。
文摘Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomatoes,Mi-1.2,fails at soil temperatures above 28℃.We cloned the heat-stable RKN-resistant gene,Mi-9,from a gene cluster composed of seven nucleotide-binding sites and leucine-rich repeat(NBS-LRR)type resistant genes in Solanum arcunum accession LA2157.Screening nematode infections in individual and combinatorial knockouts of five NBS-LRR genes showed that Mi-9 Candidate 4(MiC-4)alone is sufficient to confer heat-stable RKN resistance.Our study identifies a new source of heat-stable resistance to RKN in tomatoes for challenging environmental conditions.We also showcase a roadmap for rapid characterization of resistance genes by combining comparative genomics and genome editing,with the potential to be utilized in other crops.
基金supported by the National Key R&D Program of China(2023YFC2604500)the National Natural Science Foundation of China(32171662,32471753 and 32171666)the Natural Science Foundation of Liaoning(2020-MS-199).
文摘Exotic plant invasions and increased atmospheric carbon dioxide(CO_(2))concentration have been determined to independently affect soil nematodes,a key component of soil biota.However,little is known about the long-term effects of these two global change factors and their interactive effects.Over three consecutive years,we cultivated invasive alien plant Xanthium strumarium and its two phylogenetically related natives under both ambient(aCO_(2))and elevated(eCO_(2))atmospheric CO_(2)concentrations,and determined the effects of the invader and natives on soil nematodes under different CO_(2)concentrations and the relevant mechanism.The abundance of total soil nematodes and that of the dominant trophic group(herbivores)were significantly affected by plant species and CO_(2)concentration,and these effects were dependent on the experimental duration,however,the Shannon-diversity of nematodes was not affected by these factors.Under aCO_(2),both invasive and native species significantly increased the total nematode abundance and that of the dominant trophic group with increasing experimental duration,and the amplitude of the increase was greater under the invader relative to the natives.The eCO_(2)increased total nematode abundance(second year)and that of the dominant trophic group(third year)under the invader,but not under the natives(or even decreased)with increasing experimental duration.Root litter had greater effects on soil nematode abundance than leaf litter and root exudates did.This study indicates that eCO_(2)would aggravate effects of invasive plants on soil nematodes by increasing abundance,and these effects would vary with the duration.
基金supported by the Construction of High Quality and Efficient Mechanized Scientific and Technological Innovation Talent Team of Characteristic Coarse Cereals in Guizhou Province,China(No.BQW[2024]009)the Research and Integrated Application of Key Technologies of Green and High Yield in Characteristic Mountain Agriculture,China(No.[2023]07)the Guizhou Provincial Science and Technology Project of China(No.[2022]091)。
文摘Plant-parasitic nematodes(PPNs)(Meloidogyne sp.,Globodera sp.,and Pratylenchus sp.)and fungi are two of the most economically important groups of organisms affecting agricultural productivity worldwide.The interactions among PPNs,biocontrol fungi,and soil ecosystem can significantly impact plant health,disease management,and ecosystem functioning.We aimed to provide a comprehensive overview of the complex relationships between PPNs and biocontrol fungi,including pathogenic and biocontrol interactions.We summarized the molecular and ecological mechanisms underlying these interactions,highlighting the key players,signaling pathways,and environmental factors that influence the interactions.We also reviewed current knowledge on fungus-based biocontrol strategies against PPNs,including the development of novel management approaches.Furthermore,we explored the prospects of nematode-fungus interactions in agriculture,including the potential applications and technologies,precision agriculture,and integrated pest management approaches.This review highlights the need for further research on nematode-fungus interactions and their impact on plant infection and productivity,with an emphasis on the development of sustainable and effective strategies for managing PPNs and enhancing plant health in agricultural ecosystems.
基金financially supported by the Shaanxi Innovation Team Project,China (2024RS-CXTD-73)the National Natural Science Foundation of China (31772136)。
文摘The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population belonging to the genus Aphelenchoides was isolated from symptomatic leaves of fuchsia plants(Fuchsia×hybrida Hort.ex Sieb.& Voss.) in Chengdu,Sichuan Province,China.Morphological and morphometric characteristics were determined using light microscopy and scanning electron microscopy.Detailed examination revealed diagnostic features consistent with A.fragariae.Three ribosomal DNA(rDNA) regions,i.e.,partial small subunit(SSU) rRNA,D2-D3 expansion segments of the large subunit(LSU) rRNA,and the internal transcribed spacer(ITS),were amplified and sequenced.Bayesian phylogenetic analyses based on these sequences placed the isolate in a well-supported monophyletic clade with reference A.fragariae specimens,clearly separated from other Aphelenchoides species.Furthermore,host-suitability assays demonstrated that this nematode population not only infects and reproduces on Fuchsia×hybrida,but also on Fragaria ananassa and Pteris vittata,two known hosts of A.fragariae.Collectively,morphological,molecular,and host-range evidence confirm the identification of this nematode as A.fragariae.To our knowledge,this represents the first molecular and morphological confirmation of A.fragariae in China,and the first report of Fuchsia×hybrida as a natural host for this species.
基金This paper was supported by Natural Science Founda-tion of Heilongjiang Province (No.C-9735).
文摘This paper summarized the history and present condition of studying and utilizing entomopathogenic nematodes at home and abroad, expounded its taxonomy, life cycle and the mechanism with symbiotic bacteria killing host insect. Taxonomy, mycelial form, pathogenic function and anti-bacteria function of symbiotic bacteria were introduced. Production and utilization of entomopathogenic nematodes, the characteristic genetic improvement by use of biological engineering technology, as well as the existing problem and applying foreground were also discussed.
