The phenotypic diversity resulting from artificial or natural selection of sheep has made a significant contribution to human civilization.Hu sheep are a local sheep breed unique to China with high reproductive rates ...The phenotypic diversity resulting from artificial or natural selection of sheep has made a significant contribution to human civilization.Hu sheep are a local sheep breed unique to China with high reproductive rates and rapid growth.Genomic selection signatures have been widely used to investigate the genetic mechanisms underlying phenotypic variation in livestock.Here,we conduct whole-genome sequencing of 207 Hu sheep and compare them with the wild ancestors of domestic sheep(Asiatic mouflon)to investigate the genetic characteristics and selection signatures of Hu sheep.Based on six signatures of selection approaches,we detect genomic regions containing genes related to reproduction(BMPR1B,BMP2,PGFS,CYP19,CAMK4,GGT5,and GNAQ),vision(ALDH1A2,SAG,and PDE6B),nervous system(NAV1),and immune response(GPR35,SH2B2,PIK3R3,and HRAS).Association analysis with a population of 1299 Hu sheep reveals that those missense mutations in the GPR35(GPR35 g.952651 A>G;GPR35 g.952496 C>T)and NAV1(NAV1 g.84216190 C>T;NAV1 g.84227412 G>A)genes are significantly associated(P<0.05)with immune and growth traits in Hu sheep,respectively.This research offers unique insights into the selection characteristics of Hu sheep and facilitates further genetic improvement and molecular investigations.展开更多
Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to veri...Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to verify the effects of volatile oil of Nardostachy chinesis Batal （Gansong）. Methods Two pulses generated by computer followed by a recovery pulse and a test pulse, the interval duration between the two pulses varied from 16 ms to 1 s, and holding potential is -80 mV to - 140 inV. The peak Na^＋ current for a given recovery time was normalized to the tully recovered peak current, and the normalized value was the plot as a function of the recovery time to study the effects of 3 ppm concentration Gansong volatile oil on recovery from use-dependent block of Navl. 5 in HEK. Results It showed that Gansong group, comparing with control group, delayed the time courses of recovery from use-dependent block [ （33.2± 5.77 ） ms for control group and （52.5± 6.08 ） ms for 3 ppm Gansong group, P 〈 0.05 ] In the presence of Gansong, inhibition of the Na^＋ current was enhanced by increasing frequency of depolarizing pulse from 56.5 ms to 16 ms. In the control group, the time course of recovery showed that recovery started at 19.5 ms and finished by 36.5 ms. In the presence of Gansong, the time course of recovery showed that recovery started at 36.5 ms and finished by 56.5 ms. Na^＋ currents recovered from the use-dependent block varying with holding potential （holding potential-dependent）. Conclusions The results suggested that Na ＋ currents recovered from the use-dependent block correlated with persistent time, holding potential. The Gansong volatile oil has inhibitive effect on the Na^＋ current recovery.展开更多
基金supported by the National Key Research and Development Program of China(2021YFD1300901,2022YFD1302000)National Natural Science Foundation of China(32260818,31960653)。
文摘The phenotypic diversity resulting from artificial or natural selection of sheep has made a significant contribution to human civilization.Hu sheep are a local sheep breed unique to China with high reproductive rates and rapid growth.Genomic selection signatures have been widely used to investigate the genetic mechanisms underlying phenotypic variation in livestock.Here,we conduct whole-genome sequencing of 207 Hu sheep and compare them with the wild ancestors of domestic sheep(Asiatic mouflon)to investigate the genetic characteristics and selection signatures of Hu sheep.Based on six signatures of selection approaches,we detect genomic regions containing genes related to reproduction(BMPR1B,BMP2,PGFS,CYP19,CAMK4,GGT5,and GNAQ),vision(ALDH1A2,SAG,and PDE6B),nervous system(NAV1),and immune response(GPR35,SH2B2,PIK3R3,and HRAS).Association analysis with a population of 1299 Hu sheep reveals that those missense mutations in the GPR35(GPR35 g.952651 A>G;GPR35 g.952496 C>T)and NAV1(NAV1 g.84216190 C>T;NAV1 g.84227412 G>A)genes are significantly associated(P<0.05)with immune and growth traits in Hu sheep,respectively.This research offers unique insights into the selection characteristics of Hu sheep and facilitates further genetic improvement and molecular investigations.
基金National Natural Science Foundation of People's Republic of China(No.30660060)
文摘Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to verify the effects of volatile oil of Nardostachy chinesis Batal （Gansong）. Methods Two pulses generated by computer followed by a recovery pulse and a test pulse, the interval duration between the two pulses varied from 16 ms to 1 s, and holding potential is -80 mV to - 140 inV. The peak Na^＋ current for a given recovery time was normalized to the tully recovered peak current, and the normalized value was the plot as a function of the recovery time to study the effects of 3 ppm concentration Gansong volatile oil on recovery from use-dependent block of Navl. 5 in HEK. Results It showed that Gansong group, comparing with control group, delayed the time courses of recovery from use-dependent block [ （33.2± 5.77 ） ms for control group and （52.5± 6.08 ） ms for 3 ppm Gansong group, P 〈 0.05 ] In the presence of Gansong, inhibition of the Na^＋ current was enhanced by increasing frequency of depolarizing pulse from 56.5 ms to 16 ms. In the control group, the time course of recovery showed that recovery started at 19.5 ms and finished by 36.5 ms. In the presence of Gansong, the time course of recovery showed that recovery started at 36.5 ms and finished by 56.5 ms. Na^＋ currents recovered from the use-dependent block varying with holding potential （holding potential-dependent）. Conclusions The results suggested that Na ＋ currents recovered from the use-dependent block correlated with persistent time, holding potential. The Gansong volatile oil has inhibitive effect on the Na^＋ current recovery.
