[ Objective] To explore the effects of different proportion of bee pollen on the water holding capacity of pork in Duroc Landrace x Yorkshire growing-finishing pigs in order to determine the optimal proportion. [ Met...[ Objective] To explore the effects of different proportion of bee pollen on the water holding capacity of pork in Duroc Landrace x Yorkshire growing-finishing pigs in order to determine the optimal proportion. [ Method] A total of 80 Duroc x Landrace x Yorkshire growing-finishing pigs, weighing (10.0 ± 1.0) kg, were randomly divided into five groups. The experimental period was 120 d including the 5-day pre-feecling period. The corn-soybean meal was not replaced during the whole period. All pigs had free access to feed and water. The pigs were fasting for 24 h but not prohibited from feed before the beginning. Group I was the control group reared with the diet not supplemented bee pollen. The pigs in group II, III ,IV and V were reared with the diet supplemented bee pollen at the concentration of 1%, 3%, 5% and 7%, respectively. After slaughtering, eye muscle was used for determination of water holding capacity through fast filter paper method, drip loss method, cooking loss method and Na- pole yield determination method. [ Resultl With the increase of the proportion of bee pollen, the water holding capacity of pork was first good and then poor. The addition of bee pollen at the concentration of 5% significantly reduced the drip loss of pork and the water holding capacity which was detected by fast filter paper method, but the cooking loss and Napole yield were not significantly influenced. [ Condmion] The bee pollen can effectively improve the water holding capacity of pork in Duroc x Landrace x Yorkshire growing-finishing pigs, and the optimal proportion is 5%.展开更多
Objective: We report the results of nuclear DNA analyses of Napoléon the First (Napoléon Bonaparte;1769-1821). Design: His genomic DNA was extracted from dandruff adherent to his hair, coming from a lock of ...Objective: We report the results of nuclear DNA analyses of Napoléon the First (Napoléon Bonaparte;1769-1821). Design: His genomic DNA was extracted from dandruff adherent to his hair, coming from a lock of his hair dating from the year of 1811. Results: We obtained the complete STR (short tandem repeats) profile of Napoléon, based on fifteen autosomal loci. On this profile, ten loci (D8S1179, D21S11, D7S820, D3S1358, TH01, D16S539, D2S1338, vWa, D18S51 and FGA) are heterozygous;the most frequent alleles in Caucasians are present for only seven (allele 8 for TPOX and allele 11 for D5S818, allele 13 for D8S1179, allele 10 for D7S820, allele 9.3 for THO1, allele 12 for D16S539 and allele 24 for FGA) of the homozygous and heterozygous loci. Conclusions: So the discriminating power of this sort of genetic profile is elevated, permitting useful comparisons to other STR profiles in the future. Finally, an analysis of fifteen Y chromosomal STRs from the dandruff of this lock of hair confirms allele values of Napoléon already obtained or deduced for the corresponding loci in previous determinations.展开更多
文摘[ Objective] To explore the effects of different proportion of bee pollen on the water holding capacity of pork in Duroc Landrace x Yorkshire growing-finishing pigs in order to determine the optimal proportion. [ Method] A total of 80 Duroc x Landrace x Yorkshire growing-finishing pigs, weighing (10.0 ± 1.0) kg, were randomly divided into five groups. The experimental period was 120 d including the 5-day pre-feecling period. The corn-soybean meal was not replaced during the whole period. All pigs had free access to feed and water. The pigs were fasting for 24 h but not prohibited from feed before the beginning. Group I was the control group reared with the diet not supplemented bee pollen. The pigs in group II, III ,IV and V were reared with the diet supplemented bee pollen at the concentration of 1%, 3%, 5% and 7%, respectively. After slaughtering, eye muscle was used for determination of water holding capacity through fast filter paper method, drip loss method, cooking loss method and Na- pole yield determination method. [ Resultl With the increase of the proportion of bee pollen, the water holding capacity of pork was first good and then poor. The addition of bee pollen at the concentration of 5% significantly reduced the drip loss of pork and the water holding capacity which was detected by fast filter paper method, but the cooking loss and Napole yield were not significantly influenced. [ Condmion] The bee pollen can effectively improve the water holding capacity of pork in Duroc x Landrace x Yorkshire growing-finishing pigs, and the optimal proportion is 5%.
文摘Objective: We report the results of nuclear DNA analyses of Napoléon the First (Napoléon Bonaparte;1769-1821). Design: His genomic DNA was extracted from dandruff adherent to his hair, coming from a lock of his hair dating from the year of 1811. Results: We obtained the complete STR (short tandem repeats) profile of Napoléon, based on fifteen autosomal loci. On this profile, ten loci (D8S1179, D21S11, D7S820, D3S1358, TH01, D16S539, D2S1338, vWa, D18S51 and FGA) are heterozygous;the most frequent alleles in Caucasians are present for only seven (allele 8 for TPOX and allele 11 for D5S818, allele 13 for D8S1179, allele 10 for D7S820, allele 9.3 for THO1, allele 12 for D16S539 and allele 24 for FGA) of the homozygous and heterozygous loci. Conclusions: So the discriminating power of this sort of genetic profile is elevated, permitting useful comparisons to other STR profiles in the future. Finally, an analysis of fifteen Y chromosomal STRs from the dandruff of this lock of hair confirms allele values of Napoléon already obtained or deduced for the corresponding loci in previous determinations.