The authors regret that there was an incorrect citation in the Table 1 of the article ＂Nanopore-based Fourth-generation DNA Sequencing Technology＂, which was published in the journal Genomics, Proteomics ＆ Bioinfor...The authors regret that there was an incorrect citation in the Table 1 of the article ＂Nanopore-based Fourth-generation DNA Sequencing Technology＂, which was published in the journal Genomics, Proteomics ＆ Bioinformatics, Issue 1, 2015. In the table, the image in the 4th row originally appeared in the article ＂Nanopore-based sequence-specific detection of duplex DNA for geno- mic profiling＂ published by Nano Letters in 2010. Therefore, Ref. [8], which was associated with the image in the current Table l, should be replaced by the reference： Singer A, Wanunu M, Morrison W, Kuhn H, Frank-Kamenetskii M, Meller A. Nanopore- based sequence specific detection of duplex DNA for genomic profiling. Nano Lett 2010; 10：738-42.＂ The copy right permission of this article has been obtained. The authors would like to apologize for any inconvenience caused.展开更多
The authors regret that there were typos in the online version of Figure 4 published in Issue 1,2015.In the figure legend boxes of panels F and G,‘‘Ni’’should be corrected to‘‘N’’for the labeling of the green ...The authors regret that there were typos in the online version of Figure 4 published in Issue 1,2015.In the figure legend boxes of panels F and G,‘‘Ni’’should be corrected to‘‘N’’for the labeling of the green curves.The figure in the printed version has been corrected.The correct Figure 4 is shown below.The authors would like to apologize for any inconvenience caused.展开更多
Albinism is a genetic disorder caused by a series of genetic abnormalities that result in a decrease in melanin.Golden yellow snakehead fish(GCAS)has been discovered while the Channa argus(CAS)breeding,but the genetic...Albinism is a genetic disorder caused by a series of genetic abnormalities that result in a decrease in melanin.Golden yellow snakehead fish(GCAS)has been discovered while the Channa argus(CAS)breeding,but the genetic mechanism that causes its albinism is not known.Transcriptome sequencing and comparison of CAS and GCAS skin using Oxford Nanopore Technologies(ONT)technology to elucidate the molecular mechanisms of snakehead albinism.27.1 G clean reads and 26,198 full-length non-redundant sequences were generated via ONT sequencing.Overall,8079 new transcripts and 7231 genes were identified by comparing and analyzing redundant removed transcripts and known reference genome annotations.The results of DEGs analysis showed that a total of 59 transcripts were differentially expressed in the two groups,including 22 up-regulated transcripts and 37 down-regulated transcripts.Five causative genes associated with albinism have been identified,including TYR(Tyrosinase)and SOX10(SRY-box transcription factor 10)(tyrosinase synthesis and metabolism),S-100(S100 calcium binding protein A1)(melanin production),NLRC3(NLR family CARD domain containing 3)(disease immunity)are significantly down-regulated in GCAS,while RhoGEFs expression(melanin synthesis)is up-regulated.Protein interaction analysis revealed that GAPDH(Glyceraldehyde-3-phosphate dehydrogenase)is the top hub gene in the regulation of skin color in C.argus.In addition,the skin of snakehead fish is regulated by metabolic(GAPDH),immune[Tumor protein p53(TP53),C-X-C motif chemokine ligand 8(CXCL8),SRC proto-oncogene,non-receptor tyrosine kinase(SRC),CD274]and neural[Notch receptor 1(NOTCH1),C-X-C motif chemokine ligand 12(CXCL12)]related genes to achieve differences in skin color.These findings in understanding the process by which albinism in fish develops.展开更多
文摘The authors regret that there was an incorrect citation in the Table 1 of the article ＂Nanopore-based Fourth-generation DNA Sequencing Technology＂, which was published in the journal Genomics, Proteomics ＆ Bioinformatics, Issue 1, 2015. In the table, the image in the 4th row originally appeared in the article ＂Nanopore-based sequence-specific detection of duplex DNA for geno- mic profiling＂ published by Nano Letters in 2010. Therefore, Ref. [8], which was associated with the image in the current Table l, should be replaced by the reference： Singer A, Wanunu M, Morrison W, Kuhn H, Frank-Kamenetskii M, Meller A. Nanopore- based sequence specific detection of duplex DNA for genomic profiling. Nano Lett 2010; 10：738-42.＂ The copy right permission of this article has been obtained. The authors would like to apologize for any inconvenience caused.
文摘The authors regret that there were typos in the online version of Figure 4 published in Issue 1,2015.In the figure legend boxes of panels F and G,‘‘Ni’’should be corrected to‘‘N’’for the labeling of the green curves.The figure in the printed version has been corrected.The correct Figure 4 is shown below.The authors would like to apologize for any inconvenience caused.
基金supported by Shandong Province Agricultural Improved Seed Engineering Project (2019LZGC013)Shandong Province Key R&D Program (2021CXGC010806).
文摘Albinism is a genetic disorder caused by a series of genetic abnormalities that result in a decrease in melanin.Golden yellow snakehead fish(GCAS)has been discovered while the Channa argus(CAS)breeding,but the genetic mechanism that causes its albinism is not known.Transcriptome sequencing and comparison of CAS and GCAS skin using Oxford Nanopore Technologies(ONT)technology to elucidate the molecular mechanisms of snakehead albinism.27.1 G clean reads and 26,198 full-length non-redundant sequences were generated via ONT sequencing.Overall,8079 new transcripts and 7231 genes were identified by comparing and analyzing redundant removed transcripts and known reference genome annotations.The results of DEGs analysis showed that a total of 59 transcripts were differentially expressed in the two groups,including 22 up-regulated transcripts and 37 down-regulated transcripts.Five causative genes associated with albinism have been identified,including TYR(Tyrosinase)and SOX10(SRY-box transcription factor 10)(tyrosinase synthesis and metabolism),S-100(S100 calcium binding protein A1)(melanin production),NLRC3(NLR family CARD domain containing 3)(disease immunity)are significantly down-regulated in GCAS,while RhoGEFs expression(melanin synthesis)is up-regulated.Protein interaction analysis revealed that GAPDH(Glyceraldehyde-3-phosphate dehydrogenase)is the top hub gene in the regulation of skin color in C.argus.In addition,the skin of snakehead fish is regulated by metabolic(GAPDH),immune[Tumor protein p53(TP53),C-X-C motif chemokine ligand 8(CXCL8),SRC proto-oncogene,non-receptor tyrosine kinase(SRC),CD274]and neural[Notch receptor 1(NOTCH1),C-X-C motif chemokine ligand 12(CXCL12)]related genes to achieve differences in skin color.These findings in understanding the process by which albinism in fish develops.
