As a well-known natural protein biomaterial,silk fibroin(SF)has shown broad application prospects in typical biomedical fields.However,the mostly used SF from Bombyx mori silkworm lacks specific cell adhesion sites an...As a well-known natural protein biomaterial,silk fibroin(SF)has shown broad application prospects in typical biomedical fields.However,the mostly used SF from Bombyx mori silkworm lacks specific cell adhesion sites and other bioactive peptide sequences,and there is still significant room for further improvement of their biological functions.Therefore,it is crucial to develop a facile and effective modification strategy for this widely researched biomaterial.In this study,the SF electrospun scaffold has been chosen as a typical SF biomaterial,and air plasma etching has been adopted as a facile nanopattern modification strategy to promote its biological functions.Results demonstrated that the plasma etching could feasibly and effectively create nano-island-like patterns on the complex surface of SF scaffolds,and the detailed nanopattern features could be easily regulated by adjusting the etching time.In addition,the mesenchymal stem cell responses have illustrated that the nanopattern modification could significantly regulate corresponding cell behaviors.Compared with the non-etched scaffold,the 10min-etched scaffolds(10E scaffold)significantly promoted stem cell proliferation and osteogenic differentiation.Moreover,10E scaffold has also been confirmed to effectively accelerate vascularization and ectopic osteogenesis in vivo using a rat subcutaneous implantation model.However,the mentioned promoting effects would be weakened or even counteracted with the increase of etching time.In conclusion,this facile modification strategy demonstrated great application potential for promoting cell proliferation and differentiation.Thus,it provided useful guidance to develop excellent SF-based scaffolds suitable for bone and other tissue engineering.展开更多
基金supported by the International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality(22520711900)National Natural Science Foundation of China(52273125,52173031)+2 种基金the Fundamental Research Funds for the Central Universities(2232024D-01)the Basic Research Project of the Science and Technology Commission of Shanghai Municipality(21JC1400100)the Oriental Talent Plan(Leading Talent Program,no.152).
文摘As a well-known natural protein biomaterial,silk fibroin(SF)has shown broad application prospects in typical biomedical fields.However,the mostly used SF from Bombyx mori silkworm lacks specific cell adhesion sites and other bioactive peptide sequences,and there is still significant room for further improvement of their biological functions.Therefore,it is crucial to develop a facile and effective modification strategy for this widely researched biomaterial.In this study,the SF electrospun scaffold has been chosen as a typical SF biomaterial,and air plasma etching has been adopted as a facile nanopattern modification strategy to promote its biological functions.Results demonstrated that the plasma etching could feasibly and effectively create nano-island-like patterns on the complex surface of SF scaffolds,and the detailed nanopattern features could be easily regulated by adjusting the etching time.In addition,the mesenchymal stem cell responses have illustrated that the nanopattern modification could significantly regulate corresponding cell behaviors.Compared with the non-etched scaffold,the 10min-etched scaffolds(10E scaffold)significantly promoted stem cell proliferation and osteogenic differentiation.Moreover,10E scaffold has also been confirmed to effectively accelerate vascularization and ectopic osteogenesis in vivo using a rat subcutaneous implantation model.However,the mentioned promoting effects would be weakened or even counteracted with the increase of etching time.In conclusion,this facile modification strategy demonstrated great application potential for promoting cell proliferation and differentiation.Thus,it provided useful guidance to develop excellent SF-based scaffolds suitable for bone and other tissue engineering.
