The recent commercialization of gene products has sparked significant interest in gene therapy,necessitating efficient and precise gene delivery via various vectors.Currently,viral vectors and lipid-based nanocarriers...The recent commercialization of gene products has sparked significant interest in gene therapy,necessitating efficient and precise gene delivery via various vectors.Currently,viral vectors and lipid-based nanocarriers are the predominant choices and have been extensively investigated and reviewed.Beyond these vectors,polymeric nanocarriers also hold the promise in therapeutic gene delivery owing to their versatile functionalities,such as improving the stability,cellar uptake and endosomal escape of nucleic acid drugs,along with precise delivery to targeted tissues.This review presents a brief overview of the status quo of the emerging polymeric nanocarriers for therapeutic gene delivery,focusing on key cationic polymers,nanocarrier types,and preparation methods.It also highlights targeted diseases,strategies to improve delivery efficiency,and potential future directions in this research area.The review is hoped to inspire the development,optimization,and clinical translation of highly efficient polymeric nanocarriers for therapeutic gene delivery.展开更多
Gliomas are the most common primary tumors of the central nervous system;among them,glioblastoma multiforme stands out as the most aggressive and lethal subtype,characterized by high therapeutic resistance and frequen...Gliomas are the most common primary tumors of the central nervous system;among them,glioblastoma multiforme stands out as the most aggressive and lethal subtype,characterized by high therapeutic resistance and frequent recurrences.Glioblastoma’s complex pathology is driven by biological and molecular factors that compromise conventional therapies,including blood-brain and bloodtumor barriers,angiogenesis,immune evasion,and aberrant signaling pathways,along with genetic drivers of drug resistance.In cancer therapy,mesoporous silica nanoparticles(MSNs)have shown promise as nanocarriers thanks to the unique attributes of their mesostructure,including large surfaces,uniform pore sizes,high loading efficiency,and flexibility of chemical modifications.Several studies have proposed MSNs to address a number of challenges facing drug delivery in gliomas,including limited penetration across the blood-brain barrier,non-specific biodistribution,and systemic adverse reactions.Moreover,MSNs can be functionalized with tumor-targeting ligands so that cancer cells are selectively taken up,while they can also release therapeutic agents in response to internal and external stimuli,enabling controlled drug delivery within tumor microenvironments.Herein,we review the integration of the MSN-based delivery approach with advances in molecular oncology to improve clinical outcomes for glioma therapeutics,while highlighting the concerns around their limited clinical translation and potential toxicity.展开更多
Growing demand for natural food preservatives has stimulated increasing interest in polyphenolic compounds.However,their application in food systems is constrained by insufficient stability during processing and stora...Growing demand for natural food preservatives has stimulated increasing interest in polyphenolic compounds.However,their application in food systems is constrained by insufficient stability during processing and storage,which can lead to reduced antioxidant and antimicrobial efficacy and,consequently,compromised preservation performance.To address these limitations,green nanocarriers—including chitosan-based particles,liposomes,and nanoemulsions—have been widely explored to enhance polyphenol stability,enable controlled release,and improve compatibility within food matrices.This review systematically summarizes the bioactive properties of polyphenols,the design principles of green nanocarriers,and their applications in the preservation of meat,fruits and vegetables,and dairy products.Current evidence indicates that polysaccharide-,protein-,lipid-,and stimuli-responsive carrier systems can effectively enhance the antioxidant,antimicrobial,and anti-browning functions of polyphenols,extend food shelf life,and reduce health and environmental risks associated with synthetic preservatives.Taken together,the rational design and application of polyphenol-loaded green nanocarriers hold promise as an effective and sustainable strategy for food preservation.展开更多
Objective:To construct a pH-responsive paclitaxel(PTX)-exosome composite nanocarrier and investigate its inhibitory effect on the proliferation of endometrial cancer cells(HEC-1A).Methods:PTX was loaded into exosomes ...Objective:To construct a pH-responsive paclitaxel(PTX)-exosome composite nanocarrier and investigate its inhibitory effect on the proliferation of endometrial cancer cells(HEC-1A).Methods:PTX was loaded into exosomes derived from adipose mesenchymal stem cells using the thin-film hydration method,and modified with polyethylene glycol-polylactic-co-glycolic acid(PEG-PLGA)to form nanocarriers(PTX-Exo-NPs).The particle size and morphology were detected by nanoparticle size and Zeta potential analyzer;drug encapsulation efficiency and drug loading capacity were determined by high-performance liquid chromatography;drug release behavior was evaluated in vitro under simulated acidic(pH 5.5)and physiological(pH 7.4)conditions;MTT assay and flow cytometry were used to detect the effects of the carrier on the proliferation,apoptosis,and cell cycle distribution of HEC-1A cells.Results:PTX-Exo-NPs exhibited a uniform spherical shape with a particle size of(128.5±5.2)nm,PTX encapsulation efficiency of 92.3%±2.1%,and drug loading capacity of 15.6%±0.8%.Drug release rate in the acidic environment(85.3%±2.1%within 72 h)was significantly higher than that in the physiological environment(48.0%±1.7%).In vitro experiments demonstrated that the proliferation inhibition rate of PTX-Exo-NPs on HEC-1A cells was higher than that of free PTX,with a lower IC50(0.64μM vs 4.70μM),and could induce cell apoptosis(apoptosis rate:28.7%±2.1%vs 14.2%±1.5%)and promote cell cycle arrest(G_2/M rate:45.3%±3.2%).Conclusion:PTX-Exo-NPs exhibit pH-responsive characteristics,which can target drug release through the acidic microenvironment,enhance the proliferation inhibition and pro-apoptotic effect on endometrial cancer cells,thus serving as a potential strategy for targeted therapy of endometrial tumors.展开更多
Nanocarrier-based drug delivery systems(nDDSs)present significant opportunities for improving disease treatment,offering advantages in drug encapsulation,solubilization,stability enhancement,and optimized pharmacokine...Nanocarrier-based drug delivery systems(nDDSs)present significant opportunities for improving disease treatment,offering advantages in drug encapsulation,solubilization,stability enhancement,and optimized pharmacokinetics and biodistribution.n DDSs,comprising lipid,polymeric,protein,and inorganic nanovehicles,can be guided by or respond to biological cues for precise disease treatment and management.Equipping nanocarriers with tissue/celltargeted ligands enables effective navigation in complex environments,while functionalization with stimuli-responsive moieties facilitates site-specific controlled release.These strategies enhance drug delivery efficiency,augment therapeutic efficacy,and reduce side effects.This article reviews recent strategies and ongoing advancements in n DDSs for targeted drug delivery and controlled release,examining lesion-targeted nanomedicines through surface modification with small molecules,peptides,antibodies,carbohydrates,or cell membranes,and controlled-release nanocarriers responding to endogenous signals such as pH,redox conditions,enzymes,or external triggers like light,temperature,and magnetism.The article also discusses perspectives on future developments.展开更多
Neurodegenerative diseases including Alzheimer’s disease,Parkinson’s disease,Huntington disease and amyotrophic lateral sclerosis throw a heavy burden on families and society. Related scientific researches make tard...Neurodegenerative diseases including Alzheimer’s disease,Parkinson’s disease,Huntington disease and amyotrophic lateral sclerosis throw a heavy burden on families and society. Related scientific researches make tardy progress. One reason is that the known pathogeny is just the tip of the iceberg. Another reason is that various physiological barriers,especially blood-brain barrier(BBB),hamper effective therapeutic substances from reaching site of action. Drugs in clinical treatment of neurodegenerative diseases are basically administered orally. And generally speaking,the brain targeting efficiency is pretty low. Nanodelivery technology brings hope for neurodegenerative diseases. The use of nanocarriers encapsulating molecules such as peptides and genomic medicine may enhance drug transport through the BBB in neurodegenerative disease and target relevant regions in the brain for regenerative processes. In this review,we discuss BBB composition and applications of nanocarriers-liposomes,nanoparticles,nanomicelles and new emerging exosomes in neurodegenerative diseases. Furthermore,the disadvantages and the potential neurotoxicity of nanocarriers according pharmacokinetics theory are also discussed.展开更多
Bacterial biofilm infections have been threatening the human’s life and health globally for a long time because they typically cause chronic and persistent infections.Traditional antibiotic therapies can hardly eradi...Bacterial biofilm infections have been threatening the human’s life and health globally for a long time because they typically cause chronic and persistent infections.Traditional antibiotic therapies can hardly eradicate biofilms in many cases,as biofilms always form a robust fortress for pathogens inside,inhibiting the penetration of drugs.To address the issues,many novel drug carriers emerged as promising strategies for biofilm treatment.Among them,stimuli-responsive nanocarriers have attracted much attentions for their intriguing physicochemical properties,such as tunable size,shape and surface chemistry,especially smart drug release characteristic.Based on the microenvironmental difference between biofilm infection sites and normal tissue,many stimuli,such as bacterial products accumulating in biofilms(enzymes,glutathione,etc.),lower pH and higher H_(2)O_(2)levels,have been employed and proved in favor of“on-demand”drug release for biofilm elimination.Additionally,external stimuli including light,heat,microwave and magnetic fields are also able to control the drug releasing behavior artificially.In this review,we summarized recent advances in stimuli-responsive nanocarriers for combating biofilm infections,and mainly,focusing on the different stimuli that trigger the drug release.展开更多
A major challenge for the efficient treatment of traumatic brain injury is the need for therapeutic molecules to cross the blood-brain barrier to enter and accumulate in brain tissue.To overcome this problem,researche...A major challenge for the efficient treatment of traumatic brain injury is the need for therapeutic molecules to cross the blood-brain barrier to enter and accumulate in brain tissue.To overcome this problem,researchers have begun to focus on nanocarriers and other brain-targeting drug delivery systems.In this review,we summarize the epidemiology,basic pathophysiology,current clinical treatment,the establishment of models,and the evaluation indicators that are commonly used for traumatic brain injury.We also report the current status of traumatic brain injury when treated with nanocarriers such as liposomes and vesicles.Nanocarriers can overcome a variety of key biological barriers,improve drug bioavailability,increase intracellular penetration and retention time,achieve drug enrichment,control drug release,and achieve brain-targeting drug delivery.However,the application of nanocarriers remains in the basic research stage and has yet to be fully translated to the clinic.展开更多
Flavonoids are among the biggest group of polyphenols, widely distributed in plant-based foods. A plethora of evidence supports the health benefits and value of flavonoids can play in the physiological function treatm...Flavonoids are among the biggest group of polyphenols, widely distributed in plant-based foods. A plethora of evidence supports the health benefits and value of flavonoids can play in the physiological function treatment and in the prevention of disease particularly in the prevention of degenerative conditions including cancers, cardiovascular and neurodegenerative diseases. Hence, flavonoids represent the active constituents of many dietary supplements and herbal remedies, as well as there is an increasing interest in this class of polyphenols as functional ingredients of beverages, food grains and dairy products. Conversely, various studies have also shown that flavonoids have some drawbacks after oral administration such as stability, bioavailability and bioefficacy. This article reviews the current status of novel nanodelivery systems including nanospheres, nanocaspsules, micro- and nanoemulsions, micelles, solid lipid nanoparticles and nanostructured lipid capsules, successfully developed for overcoming the delivery challenges of flavonoids.展开更多
Grains are one of the major food staples in the world.The cereal grains are easily susceptible to damage by moisture content,flour beetles and food pathogens during storage,harvesting and post harvesting.Food preserva...Grains are one of the major food staples in the world.The cereal grains are easily susceptible to damage by moisture content,flour beetles and food pathogens during storage,harvesting and post harvesting.Food preservative techniques namely drying,freezing,and dehydration,acquire little advantages.However,they cause few undesirable alterations in the organoleptic and nutritional properties of the preserved food items.Therefore,there is a continuous search for new preservation techniques in food industries,to satisfy the customer demands on the addition of natural food preservatives,devoid of pathogenic contaminants and without changes in organoleptic properties.Essential oils(EOs)have been predicted as“natural food additives”in the preservative process.The synergistic potential of EOs with various nanocarriers plays an emerging role in the food industry.Therefore,the present review has focused on the lipid based nanocarriers,and the methods used for the functionalization or encapsulation of EOs and applications in the preservation of food items such as cooked rice,rice flour,grains,sliced breads have also been discussed.The present review ascertains the antimicrobial significance of active EOs loaded lipid nanocarriers in the form of nano emulsions,solid lipid nanoparticles and liposomes for preserving grains and flours.展开更多
Colon cancer is the fifth most common type of cancer in the world.Colon cancer develops when healthy cells in the lining of the colon or rectum alter and grow uncontrollably to form a mass known as a tumor.Despite maj...Colon cancer is the fifth most common type of cancer in the world.Colon cancer develops when healthy cells in the lining of the colon or rectum alter and grow uncontrollably to form a mass known as a tumor.Despite major medical improvements,colon cancer is still one of the leading causes of cancer-related mortality globally.One of the main issues of chemotherapy is toxicity related to conventional medicines.The targeted delivery systems are considered the safest and most effective by increasing the concentration of a therapeutic substance at the tumor site while decreasing it at other organs.Therefore,these delivery systems required lower doses for high therapeutic value with minimum side effects.The current review focuses on targeting therapeutic substances at the desired site using nanocarriers.Additionally,the diagnostic applications of nanocarriers in colorectal cancer are also discussed.展开更多
Osteoarthritis(OA),a common disabling joint disease,is highly associated with microenvironmental changes in the cartilage and subchondral bone.Elevated reactive oxygen species(ROS)in the cartilage and subchondral bone...Osteoarthritis(OA),a common disabling joint disease,is highly associated with microenvironmental changes in the cartilage and subchondral bone.Elevated reactive oxygen species(ROS)in the cartilage and subchondral bone angiogenesis accelerate articular cartilage erosion.New cartilage-targeting drug deliv-ery systems that are aimed at preventing ROS production and angiogenesis may be of clinical significance for OA treatment.Herein,an ROS scavenger and an inflammatory-responsive nanocarrier are designed by immobilizing the natural polyphenol(curcumin)in chitosan-catechol nanoformulations(Cur-CS-C NPs)via boronate ester.The robust cartilage-targeting effects and ROS scavenging capacities of Cur-CS-C NPs were respectively determined in cartilage explants and chondrocytes.Intra-articular injection of Cur-CS-C NPs in OA rat models efficiently suppressed angiogenesis and cartilage degradation partially via the ROS-mediated NF-κB/PI3K-Akt signaling pathway.The developed curcumin-functionalized nanocarriers can significantly delay OA progression and provide a promising therapeutic strategy for other inflamma-tory diseases that are characterized by oxidative stress and angiogenesis.展开更多
Exosomes as authigenous nanovesicles secreted by living cells represent a significant class of biomaterials.By virtue of their unique roles in intercellular communication,exosomes can mediate intercellular information...Exosomes as authigenous nanovesicles secreted by living cells represent a significant class of biomaterials.By virtue of their unique roles in intercellular communication,exosomes can mediate intercellular information/cargoes exchange as messenger and facilitate drug delivery as smart vehicles.Oral medication is the most clinically relied upon route of administration and can achieve both topical and systemic therapeutic effects after absorption.Exosomes and exosome-derived vectors have shown to be of high value in oral drug delivery,since they enable efficient oral delivery of therapeutic molecules by targeting intestinal epithelial cells.In recent years,exosome-biomimetic nanocarriers have emerged as an important catalyzer in innovating oral drug delivery systems.In this work,we roundly reviewed the biogenesis and functions of exosomes,their extraction and characterization methods,resources available for exosomes harvest,and design philosophy of exosome-derived vehicles,particularly highlighting the oral delivery application of exosome-biomimetic nanocarriers for diverse medicines.Accumulating evidence suggests that exosome-biomimetic nanocarriers hold great promise for oral delivery of intractable drugs with potential biopharmaceutic issues.展开更多
Nanotechnology has changed the entire paradigm of drug targeting and has shown tremendous potential in the area of cancer therapy due to its specificity. In cancer, several targets have been explored which could be ut...Nanotechnology has changed the entire paradigm of drug targeting and has shown tremendous potential in the area of cancer therapy due to its specificity. In cancer, several targets have been explored which could be utilized for the better treatment of disease. Mitochondria, the so-called powerhouse of cell, portrays significant role in the survival and death of cells, and has emerged as potential target for cancer therapy. Direct targeting and nanotechnology based approaches can be tailor-made to target mitochondria and thus improve the survival rate of patients suffering from cancer. With this backdrop, in present review, we have reemphasized the role of mitochondria in cancer progression and inhibition, highlighting the different targets that can be explored for targeting of disease. Moreover, we have also summarized different nanoparticulate systems that have been used for treatment of cancer via mitochondrial targeting.展开更多
Background:Glioblastoma multiforme(GBM)is recognized as the most lethal and most highly invasive tumor.The high likelihood of treatment failure arises fromthe presence of the blood-brain barrier(BBB)and stemcells arou...Background:Glioblastoma multiforme(GBM)is recognized as the most lethal and most highly invasive tumor.The high likelihood of treatment failure arises fromthe presence of the blood-brain barrier(BBB)and stemcells around GBM,which avert the entry of chemotherapeutic drugs into the tumormass.Objective:Recently,several researchers have designed novel nanocarrier systems like liposomes,dendrimers,metallic nanoparticles,nanodiamonds,and nanorobot approaches,allowing drugs to infiltrate the BBB more efficiently,opening up innovative avenues to prevail over therapy problems and radiation therapy.Methods:Relevant literature for this manuscript has been collected from a comprehensive and systematic search of databases,for example,PubMed,Science Direct,Google Scholar,and others,using specific keyword combinations,including“glioblastoma,”“brain tumor,”“nanocarriers,”and several others.Conclusion:This review also provides deep insights into recent advancements in nanocarrier-based formulations and technologies for GBM management.Elucidation of various scientific advances in conjunction with encouraging findings concerning the future perspectives and challenges of nanocarriers for effective brain tumor management has also been discussed.展开更多
Cancer has become a very serious challenge with aging of the human population.Advances in nanotechnology have provided new perspectives in the treatment of cancer.Through the combination of nanotechnology and therapeu...Cancer has become a very serious challenge with aging of the human population.Advances in nanotechnology have provided new perspectives in the treatment of cancer.Through the combination of nanotechnology and therapeutics,nanomedicine has been successfully used to treat cancer in recent years.In terms of nanomedicine,nanocarriers play a key role in delivering therapeutic agents,reducing severe side effects,simplifying the administration scheme,and improving therapeutic efficacies.Modulations of the structure and function of nanocarriers for improved therapeutic efficacy in cancer have attracted increasing attention in recent years.Stimuli-responsive nanocarriers penetrate deeply into tissues and respond to external or internal stimuli by releasing the therapeutic agent for cancer therapy.Notably,stimuli-responsive nanocarriers reduce the severe side effects of therapeutic agents,when compared with systemic chemotherapy,and achieve controlled drug release at tumor sites.Therefore,the development of stimuli-responsive nanocarriers plays a crucial role in drug delivery for cancer therapy.This article focuses on the development of nanomaterials with stimuli-responsive properties for use as nanocarriers,in the last few decades.These nanocarriers are more effective at delivering the therapeutic agent under the control of external or internal stimuli.Furthermore,nanocarriers with theranostic features have been designed and fabricated to confirm their great potential in achieving effective treatment of cancer,which will provide us with better choices for cancer therapy.展开更多
Well-developed mitochondria-targeted nanocarriers for function regulation are highly desirable.Numerous studies have been conducted on the treatment of mitochondria-related diseases;however,further improvements are re...Well-developed mitochondria-targeted nanocarriers for function regulation are highly desirable.Numerous studies have been conducted on the treatment of mitochondria-related diseases;however,further improvements are required to develop more effective drug delivery methods.Herein,we comprehensively introduce recent developments progress in rational design of mitochondria-targeted nanocarriers,and discuss the different strategies of available nanocarriers for targeting mitochondria.We also highlight the advantages and disadvantages of various carrier systems that are currently in use.Finally,perspective on new generation for mitochondria-targeted delivery systems in the emerging area of drug-based therapeutics is also discussed.展开更多
Chitosan-based nanocarriers(CS-NCs)show a promising role in improving drugs and bioactive compounds delivery for therapy.However,the effects exerted by CS-NCs at the cellular level,including their recognition and upta...Chitosan-based nanocarriers(CS-NCs)show a promising role in improving drugs and bioactive compounds delivery for therapy.However,the effects exerted by CS-NCs at the cellular level,including their recognition and uptake,have not been fully investigated yet.Many factors,including size,shape,concentration,and surface chemistry of CS-NCs,play an important role in determining the types of intracellular signals triggered.The mechanism of uptake and the involvement of the cytoskeleton during the CS-NCs endocytosis variates among the different cell types as well as further effects observed inside cells.In the present work,we discuss the effects induced by CS-NCs per se on the cytoskeleton,a key component in cell architecture and physiology.The focus of this report is made on tumoral and normal biological models in which CS-NCs could differentially affect the cell cytoskeleton.The recent years reports regarding the impact of CS-NCs on cytoskeleton dynamics and the current techniques for its evaluation are summarized and discussed.Understanding mechanisms underlying cytoskeletal impact after cell exposure to CS-NCs is critical for the design of safest value-added formulations in the biomedical field.Furthermore,this revision points out some interesting aspects of cytoskeletal changes and cell death encompassing anti-tumoral effects.展开更多
Despite the current advances in the discovery of the colorectal cancer biomarkers and in the diagnosis and treatment of CRC, the incidence and mortality of colon cancer are still increasing year by year. At present, m...Despite the current advances in the discovery of the colorectal cancer biomarkers and in the diagnosis and treatment of CRC, the incidence and mortality of colon cancer are still increasing year by year. At present, many patients with colon cancer have been diagnosed in an advanced stage, which always accompanied by liver metastasis and distant metastasis. However, current treatments for advanced colon cancer are limited. Therefore, the delivery of siRNA/miRNA to colon cancer lesions may be a new approach to the treatment of colon cancer. This review provides ideas for the molecular treatment of colon cancer by discussing the biological characteristics of colon cancer and the regulation of important miRNA molecules in colon cancer. Moreover, this review describes the development of nanocarriers in recent years and the important role of nanocarrier materials in the targeted delivery of small molecules. On the one hand, nanocarriers can serve as small carriers or siRNA/miRNAs, and on the other hand, can transport small molecules or siRNA/miRNAs into colon cancer cells, thereby regulating key genes expression affecting colon cancer cell proliferation, metastasis, and apoptosis. Delivery of siRNA/miRNA via nanocarriers may be a novel approach to the treatment of colon cancer.展开更多
Gene therapy provides a promising approach in treating cancers with high efficacy and selectivity and few adverse effects.Currently,the development of functional vectors with safety and effectiveness is the intense fo...Gene therapy provides a promising approach in treating cancers with high efficacy and selectivity and few adverse effects.Currently,the development of functional vectors with safety and effectiveness is the intense focus for improving the delivery of nucleic acid drugs for gene therapy.For this purpose,stimuli-responsive nanocarriers displayed strong potential in improving the overall efficiencies of gene therapy and reducing adverse effects via effective protection,prolonged blood circulation,specific tumor accumulation,and controlled release profile of nucleic acid drugs.Besides,synergistic therapy could be achieved when combined with other therapeutic regimens.This review summarizes recent advances in various stimuliresponsive nanocarriers for gene delivery.Particularly,the nanocarriers responding to endogenous stimuli including pH,reactive oxygen species,glutathione,and enzyme,etc.,and exogenous stimuli including light,thermo,ultrasound,magnetic field,etc.,are introduced.Finally,the future challenges and prospects of stimuli-responsive gene delivery nanocarriers toward potential clinical translation are well discussed.The major objective of this review is to present the biomedical potential of stimuli-responsive gene delivery nanocarriers for cancer therapy and provide guidance for developing novel nanoplatforms that are clinically applicable.展开更多
基金supported by National Natural Science Foundation of China(82104082)Natural Science Foundation of Qinghai Province(2024-ZJ-911).
文摘The recent commercialization of gene products has sparked significant interest in gene therapy,necessitating efficient and precise gene delivery via various vectors.Currently,viral vectors and lipid-based nanocarriers are the predominant choices and have been extensively investigated and reviewed.Beyond these vectors,polymeric nanocarriers also hold the promise in therapeutic gene delivery owing to their versatile functionalities,such as improving the stability,cellar uptake and endosomal escape of nucleic acid drugs,along with precise delivery to targeted tissues.This review presents a brief overview of the status quo of the emerging polymeric nanocarriers for therapeutic gene delivery,focusing on key cationic polymers,nanocarrier types,and preparation methods.It also highlights targeted diseases,strategies to improve delivery efficiency,and potential future directions in this research area.The review is hoped to inspire the development,optimization,and clinical translation of highly efficient polymeric nanocarriers for therapeutic gene delivery.
文摘Gliomas are the most common primary tumors of the central nervous system;among them,glioblastoma multiforme stands out as the most aggressive and lethal subtype,characterized by high therapeutic resistance and frequent recurrences.Glioblastoma’s complex pathology is driven by biological and molecular factors that compromise conventional therapies,including blood-brain and bloodtumor barriers,angiogenesis,immune evasion,and aberrant signaling pathways,along with genetic drivers of drug resistance.In cancer therapy,mesoporous silica nanoparticles(MSNs)have shown promise as nanocarriers thanks to the unique attributes of their mesostructure,including large surfaces,uniform pore sizes,high loading efficiency,and flexibility of chemical modifications.Several studies have proposed MSNs to address a number of challenges facing drug delivery in gliomas,including limited penetration across the blood-brain barrier,non-specific biodistribution,and systemic adverse reactions.Moreover,MSNs can be functionalized with tumor-targeting ligands so that cancer cells are selectively taken up,while they can also release therapeutic agents in response to internal and external stimuli,enabling controlled drug delivery within tumor microenvironments.Herein,we review the integration of the MSN-based delivery approach with advances in molecular oncology to improve clinical outcomes for glioma therapeutics,while highlighting the concerns around their limited clinical translation and potential toxicity.
基金supported by Doctoral research start-up fund project of Liaoning Provincial Department of Science and Technology(2025-BS-0757).
文摘Growing demand for natural food preservatives has stimulated increasing interest in polyphenolic compounds.However,their application in food systems is constrained by insufficient stability during processing and storage,which can lead to reduced antioxidant and antimicrobial efficacy and,consequently,compromised preservation performance.To address these limitations,green nanocarriers—including chitosan-based particles,liposomes,and nanoemulsions—have been widely explored to enhance polyphenol stability,enable controlled release,and improve compatibility within food matrices.This review systematically summarizes the bioactive properties of polyphenols,the design principles of green nanocarriers,and their applications in the preservation of meat,fruits and vegetables,and dairy products.Current evidence indicates that polysaccharide-,protein-,lipid-,and stimuli-responsive carrier systems can effectively enhance the antioxidant,antimicrobial,and anti-browning functions of polyphenols,extend food shelf life,and reduce health and environmental risks associated with synthetic preservatives.Taken together,the rational design and application of polyphenol-loaded green nanocarriers hold promise as an effective and sustainable strategy for food preservation.
文摘Objective:To construct a pH-responsive paclitaxel(PTX)-exosome composite nanocarrier and investigate its inhibitory effect on the proliferation of endometrial cancer cells(HEC-1A).Methods:PTX was loaded into exosomes derived from adipose mesenchymal stem cells using the thin-film hydration method,and modified with polyethylene glycol-polylactic-co-glycolic acid(PEG-PLGA)to form nanocarriers(PTX-Exo-NPs).The particle size and morphology were detected by nanoparticle size and Zeta potential analyzer;drug encapsulation efficiency and drug loading capacity were determined by high-performance liquid chromatography;drug release behavior was evaluated in vitro under simulated acidic(pH 5.5)and physiological(pH 7.4)conditions;MTT assay and flow cytometry were used to detect the effects of the carrier on the proliferation,apoptosis,and cell cycle distribution of HEC-1A cells.Results:PTX-Exo-NPs exhibited a uniform spherical shape with a particle size of(128.5±5.2)nm,PTX encapsulation efficiency of 92.3%±2.1%,and drug loading capacity of 15.6%±0.8%.Drug release rate in the acidic environment(85.3%±2.1%within 72 h)was significantly higher than that in the physiological environment(48.0%±1.7%).In vitro experiments demonstrated that the proliferation inhibition rate of PTX-Exo-NPs on HEC-1A cells was higher than that of free PTX,with a lower IC50(0.64μM vs 4.70μM),and could induce cell apoptosis(apoptosis rate:28.7%±2.1%vs 14.2%±1.5%)and promote cell cycle arrest(G_2/M rate:45.3%±3.2%).Conclusion:PTX-Exo-NPs exhibit pH-responsive characteristics,which can target drug release through the acidic microenvironment,enhance the proliferation inhibition and pro-apoptotic effect on endometrial cancer cells,thus serving as a potential strategy for targeted therapy of endometrial tumors.
基金supported by the National Natural Science Foundation of China(No.82273876)the Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China(No.171028)+1 种基金the Project of State Key Laboratory of Advanced Drug Delivery and Release Systems(No.DSQZZD-200301)the Fundamental Research Fund for the Central Universities(No.2632022YC02)。
文摘Nanocarrier-based drug delivery systems(nDDSs)present significant opportunities for improving disease treatment,offering advantages in drug encapsulation,solubilization,stability enhancement,and optimized pharmacokinetics and biodistribution.n DDSs,comprising lipid,polymeric,protein,and inorganic nanovehicles,can be guided by or respond to biological cues for precise disease treatment and management.Equipping nanocarriers with tissue/celltargeted ligands enables effective navigation in complex environments,while functionalization with stimuli-responsive moieties facilitates site-specific controlled release.These strategies enhance drug delivery efficiency,augment therapeutic efficacy,and reduce side effects.This article reviews recent strategies and ongoing advancements in n DDSs for targeted drug delivery and controlled release,examining lesion-targeted nanomedicines through surface modification with small molecules,peptides,antibodies,carbohydrates,or cell membranes,and controlled-release nanocarriers responding to endogenous signals such as pH,redox conditions,enzymes,or external triggers like light,temperature,and magnetism.The article also discusses perspectives on future developments.
基金supported by National Natural Science Foundation of China (81620108028)
文摘Neurodegenerative diseases including Alzheimer’s disease,Parkinson’s disease,Huntington disease and amyotrophic lateral sclerosis throw a heavy burden on families and society. Related scientific researches make tardy progress. One reason is that the known pathogeny is just the tip of the iceberg. Another reason is that various physiological barriers,especially blood-brain barrier(BBB),hamper effective therapeutic substances from reaching site of action. Drugs in clinical treatment of neurodegenerative diseases are basically administered orally. And generally speaking,the brain targeting efficiency is pretty low. Nanodelivery technology brings hope for neurodegenerative diseases. The use of nanocarriers encapsulating molecules such as peptides and genomic medicine may enhance drug transport through the BBB in neurodegenerative disease and target relevant regions in the brain for regenerative processes. In this review,we discuss BBB composition and applications of nanocarriers-liposomes,nanoparticles,nanomicelles and new emerging exosomes in neurodegenerative diseases. Furthermore,the disadvantages and the potential neurotoxicity of nanocarriers according pharmacokinetics theory are also discussed.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.51873212 and 51803212)the Youth Innovation Promotion Association of Chinese Academy Science(No.2017269)+1 种基金the Natural Science Foundation of Shandong Province(No.ZR2019MEM008)Chinese Academy of Sciences-Wego Group Hightech Research&Development Program.
文摘Bacterial biofilm infections have been threatening the human’s life and health globally for a long time because they typically cause chronic and persistent infections.Traditional antibiotic therapies can hardly eradicate biofilms in many cases,as biofilms always form a robust fortress for pathogens inside,inhibiting the penetration of drugs.To address the issues,many novel drug carriers emerged as promising strategies for biofilm treatment.Among them,stimuli-responsive nanocarriers have attracted much attentions for their intriguing physicochemical properties,such as tunable size,shape and surface chemistry,especially smart drug release characteristic.Based on the microenvironmental difference between biofilm infection sites and normal tissue,many stimuli,such as bacterial products accumulating in biofilms(enzymes,glutathione,etc.),lower pH and higher H_(2)O_(2)levels,have been employed and proved in favor of“on-demand”drug release for biofilm elimination.Additionally,external stimuli including light,heat,microwave and magnetic fields are also able to control the drug releasing behavior artificially.In this review,we summarized recent advances in stimuli-responsive nanocarriers for combating biofilm infections,and mainly,focusing on the different stimuli that trigger the drug release.
基金supported by the Natural Science Foundation of Beijing,No.L222126(to LD)。
文摘A major challenge for the efficient treatment of traumatic brain injury is the need for therapeutic molecules to cross the blood-brain barrier to enter and accumulate in brain tissue.To overcome this problem,researchers have begun to focus on nanocarriers and other brain-targeting drug delivery systems.In this review,we summarize the epidemiology,basic pathophysiology,current clinical treatment,the establishment of models,and the evaluation indicators that are commonly used for traumatic brain injury.We also report the current status of traumatic brain injury when treated with nanocarriers such as liposomes and vesicles.Nanocarriers can overcome a variety of key biological barriers,improve drug bioavailability,increase intracellular penetration and retention time,achieve drug enrichment,control drug release,and achieve brain-targeting drug delivery.However,the application of nanocarriers remains in the basic research stage and has yet to be fully translated to the clinic.
文摘Flavonoids are among the biggest group of polyphenols, widely distributed in plant-based foods. A plethora of evidence supports the health benefits and value of flavonoids can play in the physiological function treatment and in the prevention of disease particularly in the prevention of degenerative conditions including cancers, cardiovascular and neurodegenerative diseases. Hence, flavonoids represent the active constituents of many dietary supplements and herbal remedies, as well as there is an increasing interest in this class of polyphenols as functional ingredients of beverages, food grains and dairy products. Conversely, various studies have also shown that flavonoids have some drawbacks after oral administration such as stability, bioavailability and bioefficacy. This article reviews the current status of novel nanodelivery systems including nanospheres, nanocaspsules, micro- and nanoemulsions, micelles, solid lipid nanoparticles and nanostructured lipid capsules, successfully developed for overcoming the delivery challenges of flavonoids.
基金the financial support from the Ministry of Science and Technology of China (2016YFA0501703)Henan Natural Science (162300410060) to Dongqing Wei+3 种基金Henan University of Technology (21450004 and 21450003)Henan Province Postdoctoral Science grant (001802029 and 001803035) to Satyavani Kaliamurthi and Gurudeeban SelvarajChina Postdoctoral Science Foundation (2018M632766) to Gurudeeban SelvarajThe Ministry of Science and Technology of China (2013BAB11B02) to Keren Gu
文摘Grains are one of the major food staples in the world.The cereal grains are easily susceptible to damage by moisture content,flour beetles and food pathogens during storage,harvesting and post harvesting.Food preservative techniques namely drying,freezing,and dehydration,acquire little advantages.However,they cause few undesirable alterations in the organoleptic and nutritional properties of the preserved food items.Therefore,there is a continuous search for new preservation techniques in food industries,to satisfy the customer demands on the addition of natural food preservatives,devoid of pathogenic contaminants and without changes in organoleptic properties.Essential oils(EOs)have been predicted as“natural food additives”in the preservative process.The synergistic potential of EOs with various nanocarriers plays an emerging role in the food industry.Therefore,the present review has focused on the lipid based nanocarriers,and the methods used for the functionalization or encapsulation of EOs and applications in the preservation of food items such as cooked rice,rice flour,grains,sliced breads have also been discussed.The present review ascertains the antimicrobial significance of active EOs loaded lipid nanocarriers in the form of nano emulsions,solid lipid nanoparticles and liposomes for preserving grains and flours.
文摘Colon cancer is the fifth most common type of cancer in the world.Colon cancer develops when healthy cells in the lining of the colon or rectum alter and grow uncontrollably to form a mass known as a tumor.Despite major medical improvements,colon cancer is still one of the leading causes of cancer-related mortality globally.One of the main issues of chemotherapy is toxicity related to conventional medicines.The targeted delivery systems are considered the safest and most effective by increasing the concentration of a therapeutic substance at the tumor site while decreasing it at other organs.Therefore,these delivery systems required lower doses for high therapeutic value with minimum side effects.The current review focuses on targeting therapeutic substances at the desired site using nanocarriers.Additionally,the diagnostic applications of nanocarriers in colorectal cancer are also discussed.
基金financially supported by the National Natural Science Foundation of China (Nos.11532004 and 11832008)the Innovation and Attracting Talents Program for College and Univer-sity (“111”Project) (No.B06023).
文摘Osteoarthritis(OA),a common disabling joint disease,is highly associated with microenvironmental changes in the cartilage and subchondral bone.Elevated reactive oxygen species(ROS)in the cartilage and subchondral bone angiogenesis accelerate articular cartilage erosion.New cartilage-targeting drug deliv-ery systems that are aimed at preventing ROS production and angiogenesis may be of clinical significance for OA treatment.Herein,an ROS scavenger and an inflammatory-responsive nanocarrier are designed by immobilizing the natural polyphenol(curcumin)in chitosan-catechol nanoformulations(Cur-CS-C NPs)via boronate ester.The robust cartilage-targeting effects and ROS scavenging capacities of Cur-CS-C NPs were respectively determined in cartilage explants and chondrocytes.Intra-articular injection of Cur-CS-C NPs in OA rat models efficiently suppressed angiogenesis and cartilage degradation partially via the ROS-mediated NF-κB/PI3K-Akt signaling pathway.The developed curcumin-functionalized nanocarriers can significantly delay OA progression and provide a promising therapeutic strategy for other inflamma-tory diseases that are characterized by oxidative stress and angiogenesis.
基金supported by Guangdong Basic and Applied Basic Research Foundation(No.2023A1515012326)Major International(Regional)Joint Research Project of National Natural Science Foundation of China(No.82020108033)+1 种基金Science and Technology Projects in Guangzhou(No.202103000091)Sanming Project of Medicine in Shenzhen(No.202211003)。
文摘Exosomes as authigenous nanovesicles secreted by living cells represent a significant class of biomaterials.By virtue of their unique roles in intercellular communication,exosomes can mediate intercellular information/cargoes exchange as messenger and facilitate drug delivery as smart vehicles.Oral medication is the most clinically relied upon route of administration and can achieve both topical and systemic therapeutic effects after absorption.Exosomes and exosome-derived vectors have shown to be of high value in oral drug delivery,since they enable efficient oral delivery of therapeutic molecules by targeting intestinal epithelial cells.In recent years,exosome-biomimetic nanocarriers have emerged as an important catalyzer in innovating oral drug delivery systems.In this work,we roundly reviewed the biogenesis and functions of exosomes,their extraction and characterization methods,resources available for exosomes harvest,and design philosophy of exosome-derived vehicles,particularly highlighting the oral delivery application of exosome-biomimetic nanocarriers for diverse medicines.Accumulating evidence suggests that exosome-biomimetic nanocarriers hold great promise for oral delivery of intractable drugs with potential biopharmaceutic issues.
基金the Department of Science and Technology and SERB (INSPIRE Grant no: IFA-LSBM-13 and EMR/2016/007966/HS) for project funds。
文摘Nanotechnology has changed the entire paradigm of drug targeting and has shown tremendous potential in the area of cancer therapy due to its specificity. In cancer, several targets have been explored which could be utilized for the better treatment of disease. Mitochondria, the so-called powerhouse of cell, portrays significant role in the survival and death of cells, and has emerged as potential target for cancer therapy. Direct targeting and nanotechnology based approaches can be tailor-made to target mitochondria and thus improve the survival rate of patients suffering from cancer. With this backdrop, in present review, we have reemphasized the role of mitochondria in cancer progression and inhibition, highlighting the different targets that can be explored for targeting of disease. Moreover, we have also summarized different nanoparticulate systems that have been used for treatment of cancer via mitochondrial targeting.
文摘Background:Glioblastoma multiforme(GBM)is recognized as the most lethal and most highly invasive tumor.The high likelihood of treatment failure arises fromthe presence of the blood-brain barrier(BBB)and stemcells around GBM,which avert the entry of chemotherapeutic drugs into the tumormass.Objective:Recently,several researchers have designed novel nanocarrier systems like liposomes,dendrimers,metallic nanoparticles,nanodiamonds,and nanorobot approaches,allowing drugs to infiltrate the BBB more efficiently,opening up innovative avenues to prevail over therapy problems and radiation therapy.Methods:Relevant literature for this manuscript has been collected from a comprehensive and systematic search of databases,for example,PubMed,Science Direct,Google Scholar,and others,using specific keyword combinations,including“glioblastoma,”“brain tumor,”“nanocarriers,”and several others.Conclusion:This review also provides deep insights into recent advancements in nanocarrier-based formulations and technologies for GBM management.Elucidation of various scientific advances in conjunction with encouraging findings concerning the future perspectives and challenges of nanocarriers for effective brain tumor management has also been discussed.
基金supported by China Postdoctoral Science Foundation(Grant No.2018M632795)the National Natural Science Foundation of China(Grant No.21704093)。
文摘Cancer has become a very serious challenge with aging of the human population.Advances in nanotechnology have provided new perspectives in the treatment of cancer.Through the combination of nanotechnology and therapeutics,nanomedicine has been successfully used to treat cancer in recent years.In terms of nanomedicine,nanocarriers play a key role in delivering therapeutic agents,reducing severe side effects,simplifying the administration scheme,and improving therapeutic efficacies.Modulations of the structure and function of nanocarriers for improved therapeutic efficacy in cancer have attracted increasing attention in recent years.Stimuli-responsive nanocarriers penetrate deeply into tissues and respond to external or internal stimuli by releasing the therapeutic agent for cancer therapy.Notably,stimuli-responsive nanocarriers reduce the severe side effects of therapeutic agents,when compared with systemic chemotherapy,and achieve controlled drug release at tumor sites.Therefore,the development of stimuli-responsive nanocarriers plays a crucial role in drug delivery for cancer therapy.This article focuses on the development of nanomaterials with stimuli-responsive properties for use as nanocarriers,in the last few decades.These nanocarriers are more effective at delivering the therapeutic agent under the control of external or internal stimuli.Furthermore,nanocarriers with theranostic features have been designed and fabricated to confirm their great potential in achieving effective treatment of cancer,which will provide us with better choices for cancer therapy.
基金financial support from the National Key Research and Development Project (No. 2020YFA0709900)the Key University Science Research Project of Jiangsu Province (No. 19KJA520005)+1 种基金the National Natural Science Foundation of China (No. 22077101)the Open Project Program of State Key Laboratory of Coordination Chemistry (No. 202008)
文摘Well-developed mitochondria-targeted nanocarriers for function regulation are highly desirable.Numerous studies have been conducted on the treatment of mitochondria-related diseases;however,further improvements are required to develop more effective drug delivery methods.Herein,we comprehensively introduce recent developments progress in rational design of mitochondria-targeted nanocarriers,and discuss the different strategies of available nanocarriers for targeting mitochondria.We also highlight the advantages and disadvantages of various carrier systems that are currently in use.Finally,perspective on new generation for mitochondria-targeted delivery systems in the emerging area of drug-based therapeutics is also discussed.
基金ANPCyT(PICTs 2015-3866 and 2017-1683)Universidad de Buenos Aires UBACyT 20020190100297BA and CONICET.
文摘Chitosan-based nanocarriers(CS-NCs)show a promising role in improving drugs and bioactive compounds delivery for therapy.However,the effects exerted by CS-NCs at the cellular level,including their recognition and uptake,have not been fully investigated yet.Many factors,including size,shape,concentration,and surface chemistry of CS-NCs,play an important role in determining the types of intracellular signals triggered.The mechanism of uptake and the involvement of the cytoskeleton during the CS-NCs endocytosis variates among the different cell types as well as further effects observed inside cells.In the present work,we discuss the effects induced by CS-NCs per se on the cytoskeleton,a key component in cell architecture and physiology.The focus of this report is made on tumoral and normal biological models in which CS-NCs could differentially affect the cell cytoskeleton.The recent years reports regarding the impact of CS-NCs on cytoskeleton dynamics and the current techniques for its evaluation are summarized and discussed.Understanding mechanisms underlying cytoskeletal impact after cell exposure to CS-NCs is critical for the design of safest value-added formulations in the biomedical field.Furthermore,this revision points out some interesting aspects of cytoskeletal changes and cell death encompassing anti-tumoral effects.
文摘Despite the current advances in the discovery of the colorectal cancer biomarkers and in the diagnosis and treatment of CRC, the incidence and mortality of colon cancer are still increasing year by year. At present, many patients with colon cancer have been diagnosed in an advanced stage, which always accompanied by liver metastasis and distant metastasis. However, current treatments for advanced colon cancer are limited. Therefore, the delivery of siRNA/miRNA to colon cancer lesions may be a new approach to the treatment of colon cancer. This review provides ideas for the molecular treatment of colon cancer by discussing the biological characteristics of colon cancer and the regulation of important miRNA molecules in colon cancer. Moreover, this review describes the development of nanocarriers in recent years and the important role of nanocarrier materials in the targeted delivery of small molecules. On the one hand, nanocarriers can serve as small carriers or siRNA/miRNAs, and on the other hand, can transport small molecules or siRNA/miRNAs into colon cancer cells, thereby regulating key genes expression affecting colon cancer cell proliferation, metastasis, and apoptosis. Delivery of siRNA/miRNA via nanocarriers may be a novel approach to the treatment of colon cancer.
基金the financial support from the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(52103196 and 52073060)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2021B1515120054)the Shenzhen Fundamental Research Program(JCYJ20190813152616459 and JCYJ20210324133214038)。
文摘Gene therapy provides a promising approach in treating cancers with high efficacy and selectivity and few adverse effects.Currently,the development of functional vectors with safety and effectiveness is the intense focus for improving the delivery of nucleic acid drugs for gene therapy.For this purpose,stimuli-responsive nanocarriers displayed strong potential in improving the overall efficiencies of gene therapy and reducing adverse effects via effective protection,prolonged blood circulation,specific tumor accumulation,and controlled release profile of nucleic acid drugs.Besides,synergistic therapy could be achieved when combined with other therapeutic regimens.This review summarizes recent advances in various stimuliresponsive nanocarriers for gene delivery.Particularly,the nanocarriers responding to endogenous stimuli including pH,reactive oxygen species,glutathione,and enzyme,etc.,and exogenous stimuli including light,thermo,ultrasound,magnetic field,etc.,are introduced.Finally,the future challenges and prospects of stimuli-responsive gene delivery nanocarriers toward potential clinical translation are well discussed.The major objective of this review is to present the biomedical potential of stimuli-responsive gene delivery nanocarriers for cancer therapy and provide guidance for developing novel nanoplatforms that are clinically applicable.
