A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework （enzyme@SNF@ZIF-8） structure with improved stability is fabricated for the first time. The versatility of this s...A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework （enzyme@SNF@ZIF-8） structure with improved stability is fabricated for the first time. The versatility of this system is validated using penicillin G acylase （PGA） and catalase （CAT） as model enzymes. The microporous ZIF-8 layer can be controlled by varying the number of ZIF-8 coating cycles, which produces PGA@SNF@ZIF-8 nanobiocatalysts with different ZIF-8 layer thicknesses. After the second ZIF-8 coating cycle, a PGA@SNF@ZIF-8（2） structure with a homogeneous and well-intergrown ZIF-8 layer is formed, which possesses excellent mechanical and chemical stability. Moreover, PGA@SNF@ZIF-8（2） shows improved thermal/storage stability and reusability compared with free PGA and PGA immobilized on silica nanoflowers （PGA@SNF）. The obtained CAT-based nanobiocatalysts （CAT@SNF@ZIF-8（2）） also show excellent catalytic performance.展开更多
Vascular grafts are commonly used to treat acute injuries and chronic atherosclerotic diseases of the vasculature.However,the pathological environment of injured vessels is characterized by oxidative stress and severe...Vascular grafts are commonly used to treat acute injuries and chronic atherosclerotic diseases of the vasculature.However,the pathological environment of injured vessels is characterized by oxidative stress and severe inflammatory flares,which usually lead to insufficient vascular regeneration and poor pathological remodeling,with far from satisfactory graft results.Here,we innovatively engineered a nanocarbon supporting porous Ru-porphyrin-based nanobiocatalyst functionalized vascular graft(SPPorRu@PCL)via electrospinning technology.Our studies demonstrate that the SPPorRu@PCL has ultrafast and broad-spectrum reactive oxygen species(ROS)scavenging ability due to the highly activeπ-conjugated Ru-N catalytic site,π-π stacking effect,and porous structure of loaded SPPorRu,which synergistically enhances its electron transfer ability and catalytic kinetics.Strikingly,in vitro cellular experiments demonstrate that the SPPorRu@PCL is effective in alleviating oxidative stress,reducing damage of DNA and mitochondrial,and promoting cell adhesion for human umbilical vein endothelial cells in a high-ROS environment.Implantation of SPPorRu@PCL in vascular-injured rats further demonstrates its superior biocompatibility,anti-inflammatory and provascular repair capabilities.This work provides important insights into the application of the porous nanocarbon and the π-conjugated porphyrin-based Ru-N coordination nanobiocatalyst assembled on nanocarbons in catalytically scavenging ROS and offers new strategies to design high-performance artificial antioxidant functionalized vascular grafts for the treatment of blood vessel injury diseases.展开更多
文摘A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework （enzyme@SNF@ZIF-8） structure with improved stability is fabricated for the first time. The versatility of this system is validated using penicillin G acylase （PGA） and catalase （CAT） as model enzymes. The microporous ZIF-8 layer can be controlled by varying the number of ZIF-8 coating cycles, which produces PGA@SNF@ZIF-8 nanobiocatalysts with different ZIF-8 layer thicknesses. After the second ZIF-8 coating cycle, a PGA@SNF@ZIF-8（2） structure with a homogeneous and well-intergrown ZIF-8 layer is formed, which possesses excellent mechanical and chemical stability. Moreover, PGA@SNF@ZIF-8（2） shows improved thermal/storage stability and reusability compared with free PGA and PGA immobilized on silica nanoflowers （PGA@SNF）. The obtained CAT-based nanobiocatalysts （CAT@SNF@ZIF-8（2）） also show excellent catalytic performance.
基金financially supported by the National Natural Science Foundation of China(82371976,82272003,52373148,82302211,82402269)the Sichuan Science and Technology Program(2024YFHZ0271,2024YFHZ0364)+2 种基金the Sichuan Provincial Youth Science Foundation(No.2025ZNSFSC1750)the Post-Doctor Research Project,West China Hospital,Sichuan University(2024HXBH051)China Postdoctoral Science Foundation(2024M762250).
文摘Vascular grafts are commonly used to treat acute injuries and chronic atherosclerotic diseases of the vasculature.However,the pathological environment of injured vessels is characterized by oxidative stress and severe inflammatory flares,which usually lead to insufficient vascular regeneration and poor pathological remodeling,with far from satisfactory graft results.Here,we innovatively engineered a nanocarbon supporting porous Ru-porphyrin-based nanobiocatalyst functionalized vascular graft(SPPorRu@PCL)via electrospinning technology.Our studies demonstrate that the SPPorRu@PCL has ultrafast and broad-spectrum reactive oxygen species(ROS)scavenging ability due to the highly activeπ-conjugated Ru-N catalytic site,π-π stacking effect,and porous structure of loaded SPPorRu,which synergistically enhances its electron transfer ability and catalytic kinetics.Strikingly,in vitro cellular experiments demonstrate that the SPPorRu@PCL is effective in alleviating oxidative stress,reducing damage of DNA and mitochondrial,and promoting cell adhesion for human umbilical vein endothelial cells in a high-ROS environment.Implantation of SPPorRu@PCL in vascular-injured rats further demonstrates its superior biocompatibility,anti-inflammatory and provascular repair capabilities.This work provides important insights into the application of the porous nanocarbon and the π-conjugated porphyrin-based Ru-N coordination nanobiocatalyst assembled on nanocarbons in catalytically scavenging ROS and offers new strategies to design high-performance artificial antioxidant functionalized vascular grafts for the treatment of blood vessel injury diseases.
