Photodynamic therapy(PDT)not only directly eradicates tumor cells but also boosts immunogenicity,promoting antigen presentation and immune cell infiltration.However,the robust antioxidant defense mechanisms within tum...Photodynamic therapy(PDT)not only directly eradicates tumor cells but also boosts immunogenicity,promoting antigen presentation and immune cell infiltration.However,the robust antioxidant defense mechanisms within tumor cells significantly weaken the efficacy of photodynamic immunotherapy.Herein,a supramolecular hybrid nanoassembly is constructed by exploring the synergistic effects of the photodynamic photosensitizer(pyropheophorbide a,PPa)and the ferroptosis inducer(erastin).The erastinmediated inhibition of system X_(c)−significantly downregulates glutathione(GSH)expression,amplifying intracellular oxidative stress,leading to pronounced cell apoptosis,and promoting the release of damageassociated molecular patterns(DAMPs).Additionally,the precise cooperation of PPa and erastin enhances ferroptosis efficiency,exacerbating the accumulation of lipid peroxides(LPOs).Ultimately,LPOs serve as a“find me”signal,while DMAPs act as an“eat me”signal,collectively promoting dendritic cell maturation,enhancing infiltration of the cytotoxic T lymphocytes,and eliciting a robust immune response.This study opens new horizons for enhancing tumor immunotherapy through simultaneous ferroptosis-PDT.展开更多
Integrating discrete plasmonic nanoparticles into assemblies can induce plasmonic coupling that produces collective plasmonic properties,which are not available for single nanoparticles.Theoretical analysis revealed t...Integrating discrete plasmonic nanoparticles into assemblies can induce plasmonic coupling that produces collective plasmonic properties,which are not available for single nanoparticles.Theoretical analysis revealed that plasmonic coupling derived from assemblies could produce stronger electromagnetic field enhancement effects.Thus,plasmonic assemblies enable better performance in plasmon-based applications,such as enhanced fluorescence and Raman effects.This makes them hold great potential for trace analyte detection and nanomedicine.Herein,we focus on the recent advances in various plasmonic nanoassembles such as dimers,tetramers,and core-satellite structures,and discuss their applications in biosensing and cell imaging.The fabrication strategies for self-assembled plasmonic nanostructures are described,including top-down strategies,self-assembly methods linked by DNA,ligand,polymer,amino acid,or proteins,and chemical overgrowth methods.Thereafter,their applications in biosensor and cell imaging based on dark-field imaging,surface-enhanced Raman scattering,plasmonic circular dichroism,and fluorescence imaging are discussed.Finally,the remaining challenges and prospects are elucidated.展开更多
基金financially supported by the National Natural Science Foundation of China(No.82161138029)the Basic Research Projects of Liaoning Provincial Department of Education(No.LJKZZ20220109)the Shenyang Youth Science and Technology Innovation Talents Program(No.RC210452).
文摘Photodynamic therapy(PDT)not only directly eradicates tumor cells but also boosts immunogenicity,promoting antigen presentation and immune cell infiltration.However,the robust antioxidant defense mechanisms within tumor cells significantly weaken the efficacy of photodynamic immunotherapy.Herein,a supramolecular hybrid nanoassembly is constructed by exploring the synergistic effects of the photodynamic photosensitizer(pyropheophorbide a,PPa)and the ferroptosis inducer(erastin).The erastinmediated inhibition of system X_(c)−significantly downregulates glutathione(GSH)expression,amplifying intracellular oxidative stress,leading to pronounced cell apoptosis,and promoting the release of damageassociated molecular patterns(DAMPs).Additionally,the precise cooperation of PPa and erastin enhances ferroptosis efficiency,exacerbating the accumulation of lipid peroxides(LPOs).Ultimately,LPOs serve as a“find me”signal,while DMAPs act as an“eat me”signal,collectively promoting dendritic cell maturation,enhancing infiltration of the cytotoxic T lymphocytes,and eliciting a robust immune response.This study opens new horizons for enhancing tumor immunotherapy through simultaneous ferroptosis-PDT.
基金supported by grants from the National Natural Science Foundation of China(Nos.22022412,22274076,21874155)the Primary Research&Development Plan of Jiangsu Province(No.BE2022793)。
文摘Integrating discrete plasmonic nanoparticles into assemblies can induce plasmonic coupling that produces collective plasmonic properties,which are not available for single nanoparticles.Theoretical analysis revealed that plasmonic coupling derived from assemblies could produce stronger electromagnetic field enhancement effects.Thus,plasmonic assemblies enable better performance in plasmon-based applications,such as enhanced fluorescence and Raman effects.This makes them hold great potential for trace analyte detection and nanomedicine.Herein,we focus on the recent advances in various plasmonic nanoassembles such as dimers,tetramers,and core-satellite structures,and discuss their applications in biosensing and cell imaging.The fabrication strategies for self-assembled plasmonic nanostructures are described,including top-down strategies,self-assembly methods linked by DNA,ligand,polymer,amino acid,or proteins,and chemical overgrowth methods.Thereafter,their applications in biosensor and cell imaging based on dark-field imaging,surface-enhanced Raman scattering,plasmonic circular dichroism,and fluorescence imaging are discussed.Finally,the remaining challenges and prospects are elucidated.
