Background:Nails are one of the objects that are more durable than other witness objects.This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to g...Background:Nails are one of the objects that are more durable than other witness objects.This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.Aims and Objectives:This study aimed to evaluate the yield and quality of DNA extracted from the nail.Materials and Methods:Nail samples from 15 males and 15 females over 18 were extracted using GeneAll®Exgene™Cell SV Mini Kit.The DNA concentration and purity were measured using a NanoDrop Spectrophotometer,and the quality of DNAwas assessed by polymerase chain reaction(PCR)using primers specific for human growth hormone(HGH).Results:The average yield of DNA was 0.816µg(0.141–2.706µg)obtained from the average nail weight of 30.2 mg(22.2–40.0 mg).The average DNA concentration was 27.2 ng/µL(4.7–90.2 ng/µL),corresponding to 30µL in volume.Almost all DNA samples(96.7%)had high purity(A260/A280≥1.80)and gave a band of PCR product of the HGH gene on agarose gel electrophoresis.Conclusion:Spin column-based nucleic acid purification is recommended for nail DNA extraction due to its simplicity and high quality.展开更多
文摘Background:Nails are one of the objects that are more durable than other witness objects.This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.Aims and Objectives:This study aimed to evaluate the yield and quality of DNA extracted from the nail.Materials and Methods:Nail samples from 15 males and 15 females over 18 were extracted using GeneAll®Exgene™Cell SV Mini Kit.The DNA concentration and purity were measured using a NanoDrop Spectrophotometer,and the quality of DNAwas assessed by polymerase chain reaction(PCR)using primers specific for human growth hormone(HGH).Results:The average yield of DNA was 0.816µg(0.141–2.706µg)obtained from the average nail weight of 30.2 mg(22.2–40.0 mg).The average DNA concentration was 27.2 ng/µL(4.7–90.2 ng/µL),corresponding to 30µL in volume.Almost all DNA samples(96.7%)had high purity(A260/A280≥1.80)and gave a band of PCR product of the HGH gene on agarose gel electrophoresis.Conclusion:Spin column-based nucleic acid purification is recommended for nail DNA extraction due to its simplicity and high quality.
