It is of great significance to develop effective antibacterial agents and methods to combat drug resistant bacterial infections due to its increasing threaten to human health and the ineffectiveness of antibiotics.Her...It is of great significance to develop effective antibacterial agents and methods to combat drug resistant bacterial infections due to its increasing threaten to human health and the ineffectiveness of antibiotics.Herein,a multifunctional hybrid nano-assembly(M1-Fe NPs)based on conjugated oligomer and ferrous ion was engineered with favorable bactericidal activity for synergetic antibacterial therapy.The chelation of ferrous ion not only enhances the photothermal conversion efficiency of M1 but also endows the nano-assembly with catalytic capability of transferring H_(2)O_(2) into stronger oxidant hydroxyl radicals(·OH).Meanwhile,the generated heat can further promote the Fenton reaction activity.By generating cytotoxic heat and oxidative·OH,M1-Fe NPs can effectively kill Staphylococcus aureus in vitro and in vivo with the aid of low dosage of H_(2)O_(2).The work provides a new multifunctional platform for combinational drug resistant antibacterial therapy and even antitumor therapy.展开更多
Lipid metabolism imbalance combined with over-activated inflammation are two key factors for hepatic stestosis.However,on-demand anchoring inflammation and lipid metabolism disorder for hepatic stestosis treatment has...Lipid metabolism imbalance combined with over-activated inflammation are two key factors for hepatic stestosis.However,on-demand anchoring inflammation and lipid metabolism disorder for hepatic stestosis treatment has yet to be realized.Here we propose a charge reversal fullerene based nano-assembly to migrate hepatic steatosis via inhibiting macrophage-mediated inflammation and normalizing hepatocellular lipid metabolism in obesity mice.Our nano-assembly(abbreviated as FPPD)is comprised of electropositive polyetherimide(PEI),charge-shielded dimethylmaleic anhydride(DMA),and poly(lactic-co-glycolic acid)(PLGA),which provides hydrophobic chains for self-assembly with anti-oxidative dicarboxy fullerene poly(ethylene glycol)molecule(FP).The obtained FPPD nano-assembly owns a charge reversal ability that switches to a positive charge in an acidic environment that targets the electronegative mitochondria both in pro-inflammatory macrophages and steatosis hepatocytes.We demonstrate that the anti-oxidative and mitochondria-targeting FPPD notably reduces inflammation in macrophages and lipid accumulation in hepatocytes by quenching excessive reactive oxygen species(ROS)and improving mitochondrial function in vitro.Importantly,FPPD nano-assembly reveals a superior anti-hepatic steatosis effect via migrating inflammation and facilitating lipid transport in obesity mice.Overall,the charge reversal nano-assembly reduces over-activated inflammation and promotes lipid metabolism that provides an effectiveness of a multi-target strategy for hepatic steatosis treatment.展开更多
A new nano-assembly approach has been proposed for the preparation of macropore volume mesoporous aluminum oxide supports.Secondary nano-assembly and a frame structure mechanism for large pore volume mesoporous suppor...A new nano-assembly approach has been proposed for the preparation of macropore volume mesoporous aluminum oxide supports.Secondary nano-assembly and a frame structure mechanism for large pore volume mesoporous supports have been proposed.In a primary nano-assembly supersoluble micelle,aluminum hydroxide nanoparticles were precipitated in situ in surfactants with a volume balance(VB)less than 1,followed by secondary nano-assembly in linear and cylindrical shapes.The secondary nano-assembly of cylindrical aluminum hydroxides was calcined to form nano cylindrical aluminum oxides.For the formation of macropore volume mesoporous supports,we utilized a frame structure mechanism of mesoporous support,in which the exterior surface of the carrier may not be continuous.This macropore volume support has been used for the hydrotreatment of a residual oil catalyst,which possesses the following physical characteristics:pore volume 1.8―2.7 mL·g-1,specific surface area 180―429 m2·g-1,average pore diameter 17―57 nm,average pore diameter more than 10 nm(81%―94%),porosity 87%―93%,and crush strength 7.7―25 N·mm-1.展开更多
The escalating issue of lung infections induced by multi-drug resistant(MDR)bacteria is threatening human health.Thus,the development of efficient drug delivery systems is essential to eliminate MDR bacterial lung inf...The escalating issue of lung infections induced by multi-drug resistant(MDR)bacteria is threatening human health.Thus,the development of efficient drug delivery systems is essential to eliminate MDR bacterial lung infections effectively.Herein,we designed inhalable drug-loaded nano-assemblies by the electrostatic interaction between negatively charged sodium alginate and a positively charged antibacterial polymer,quaternized polyethyleneimine(QPEI-C_(6)),as well as a kind of typical antibiotic for therapy of lung infection,azithromycin(AZT).By adjusting the feed ratios,we optimized the size of the nano-assembly to approximately 200 nm(STQ_(12)),which was beneficial for penetration through the mucus layer and biofilm.In the slightly acidic environment of the infected site,the nano-assembly could dissemble responsively and release AZT and QPEI-C_(6).Because of the combined bactericidal effect,STQ_(12)exhibited high bactericidal efficiency against MDR bacteria.In animal experiments,STQ_(12)showed notable efficacy against MDR bacterial lung infection.Gene transcriptomic results showed that the main effects of STQ_(12)against bacteria were through influencing the bacterial cell components and metabolic processes,and affecting their growth and reproduction.This work provides a promising strategy to treat MDR bacterium-induced lower respiratory tract infections.展开更多
Nano assembly and manipulation technologies are the basis for nano-electro-mechanical systems（NEMS）. Atomic force microscope（AFM） is widely used to manipulate nanotubes to assemble NEMS. Manipulating nanotubes wit...Nano assembly and manipulation technologies are the basis for nano-electro-mechanical systems（NEMS）. Atomic force microscope（AFM） is widely used to manipulate nanotubes to assemble NEMS. Manipulating nanotubes with AFM is a challenging and difficult task. One of the major reasons is the deficiency of visual information during the manipulation process. To address these difficulties, this research aims to put forward novel virtual tools and assembly strategies to improve the efficiency, accuracy and ease of the assembly process of NEMS. This paper begins by the discussion of the principles and implementation of a virtual nano-assembly simulator, which serves as a benchmark to test the proposed NEMS assembly techniques and virtual tools. Then, a general framework of nanotube-based NEMS assembly is proposed. Several nano-assembly strategies and virtual tools, such as automated path planning for NEMS assembly, a four-step scheme of nanotube manipulation, virtual fixtures for assembly finalization and safe manipulation, are introduced. These virtual tools and methods are experimented for justification. An assembly task of moderate complexity was performed in our virtual nano-assembly simulator with and without the help of the proposed toolkit. Experimental results suggest that the proposed methods tend to greatly enhance the efficiency and accuracy of nanotube-based NEMS assembly. In general, the proposed virtual reality toolkit not only ensures the safety, but also enhances the accuracy and efficiency of the assembly of nanotube-based NEMS.展开更多
Nowadays, fabrication of micro/nano-scale electronic devices with bottom-up approach is paid much research attention. Here, we provide a novel micro/nano-assembling method, which is accurate and efficient, especially ...Nowadays, fabrication of micro/nano-scale electronic devices with bottom-up approach is paid much research attention. Here, we provide a novel micro/nano-assembling method, which is accurate and efficient, especially suitable for the fabrication of micro/nano-scale electronic devices. Using this method, a self-powered ZnO/Sb-doped ZnO nanowire p–n homojunction ultraviolet detector(UVD) was fabricated, and the detailed photoelectric properties were tested. At a reverse bias of -0.1 V under UV light illumination, the photoresponse sensitivity of the UVD was 26.5 and the rise/decay time of the UVD was as short as 30 ms. The micro/nano-assembling method has wide potential applications in the fabrication of specific micro/nano-scale electronic devices.展开更多
基金the National Natural Science Foundation of China(Nos.21977065,22177065 and 21807067)Sanjin Scholars Support Planunder Special Funding(No.2017-06)+2 种基金Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(Nos.201802106,2019L0022)Supported by the Fund for Shanxi“1331”Project(1331)the Program for Introducing Overseas High-level Talents of Shanxi(Hundred Talents Plan)。
文摘It is of great significance to develop effective antibacterial agents and methods to combat drug resistant bacterial infections due to its increasing threaten to human health and the ineffectiveness of antibiotics.Herein,a multifunctional hybrid nano-assembly(M1-Fe NPs)based on conjugated oligomer and ferrous ion was engineered with favorable bactericidal activity for synergetic antibacterial therapy.The chelation of ferrous ion not only enhances the photothermal conversion efficiency of M1 but also endows the nano-assembly with catalytic capability of transferring H_(2)O_(2) into stronger oxidant hydroxyl radicals(·OH).Meanwhile,the generated heat can further promote the Fenton reaction activity.By generating cytotoxic heat and oxidative·OH,M1-Fe NPs can effectively kill Staphylococcus aureus in vitro and in vivo with the aid of low dosage of H_(2)O_(2).The work provides a new multifunctional platform for combinational drug resistant antibacterial therapy and even antitumor therapy.
基金supported by the National Key R&D Program of China(Grant No.2022YFA1205900)the National Natural Science Foundation of China(52172055)+1 种基金the Key Research Program of the Chinese Academy of Sciences(QYZDJ-SSW-SLH01)Mingming Zhen particularly thanks the Youth Innovation Promotion Association of CAS(2022036).
文摘Lipid metabolism imbalance combined with over-activated inflammation are two key factors for hepatic stestosis.However,on-demand anchoring inflammation and lipid metabolism disorder for hepatic stestosis treatment has yet to be realized.Here we propose a charge reversal fullerene based nano-assembly to migrate hepatic steatosis via inhibiting macrophage-mediated inflammation and normalizing hepatocellular lipid metabolism in obesity mice.Our nano-assembly(abbreviated as FPPD)is comprised of electropositive polyetherimide(PEI),charge-shielded dimethylmaleic anhydride(DMA),and poly(lactic-co-glycolic acid)(PLGA),which provides hydrophobic chains for self-assembly with anti-oxidative dicarboxy fullerene poly(ethylene glycol)molecule(FP).The obtained FPPD nano-assembly owns a charge reversal ability that switches to a positive charge in an acidic environment that targets the electronegative mitochondria both in pro-inflammatory macrophages and steatosis hepatocytes.We demonstrate that the anti-oxidative and mitochondria-targeting FPPD notably reduces inflammation in macrophages and lipid accumulation in hepatocytes by quenching excessive reactive oxygen species(ROS)and improving mitochondrial function in vitro.Importantly,FPPD nano-assembly reveals a superior anti-hepatic steatosis effect via migrating inflammation and facilitating lipid transport in obesity mice.Overall,the charge reversal nano-assembly reduces over-activated inflammation and promotes lipid metabolism that provides an effectiveness of a multi-target strategy for hepatic steatosis treatment.
文摘A new nano-assembly approach has been proposed for the preparation of macropore volume mesoporous aluminum oxide supports.Secondary nano-assembly and a frame structure mechanism for large pore volume mesoporous supports have been proposed.In a primary nano-assembly supersoluble micelle,aluminum hydroxide nanoparticles were precipitated in situ in surfactants with a volume balance(VB)less than 1,followed by secondary nano-assembly in linear and cylindrical shapes.The secondary nano-assembly of cylindrical aluminum hydroxides was calcined to form nano cylindrical aluminum oxides.For the formation of macropore volume mesoporous supports,we utilized a frame structure mechanism of mesoporous support,in which the exterior surface of the carrier may not be continuous.This macropore volume support has been used for the hydrotreatment of a residual oil catalyst,which possesses the following physical characteristics:pore volume 1.8―2.7 mL·g-1,specific surface area 180―429 m2·g-1,average pore diameter 17―57 nm,average pore diameter more than 10 nm(81%―94%),porosity 87%―93%,and crush strength 7.7―25 N·mm-1.
基金Beijing Outstanding Young Scientist Program,Grant/Award Number:BJJWZYJH01201910010024National Natural Science Foundation of China,Grant/Award Numbers:52073024,52122304,52221006,52293382Beijing Municipal Science and Technology Project,Grant/Award Number:Z191100006619099。
文摘The escalating issue of lung infections induced by multi-drug resistant(MDR)bacteria is threatening human health.Thus,the development of efficient drug delivery systems is essential to eliminate MDR bacterial lung infections effectively.Herein,we designed inhalable drug-loaded nano-assemblies by the electrostatic interaction between negatively charged sodium alginate and a positively charged antibacterial polymer,quaternized polyethyleneimine(QPEI-C_(6)),as well as a kind of typical antibiotic for therapy of lung infection,azithromycin(AZT).By adjusting the feed ratios,we optimized the size of the nano-assembly to approximately 200 nm(STQ_(12)),which was beneficial for penetration through the mucus layer and biofilm.In the slightly acidic environment of the infected site,the nano-assembly could dissemble responsively and release AZT and QPEI-C_(6).Because of the combined bactericidal effect,STQ_(12)exhibited high bactericidal efficiency against MDR bacteria.In animal experiments,STQ_(12)showed notable efficacy against MDR bacterial lung infection.Gene transcriptomic results showed that the main effects of STQ_(12)against bacteria were through influencing the bacterial cell components and metabolic processes,and affecting their growth and reproduction.This work provides a promising strategy to treat MDR bacterium-induced lower respiratory tract infections.
基金supported by National Important Project on Science&Technology of China(Grant No.2009ZX04014-031)Specialized Research Fund for the Doctoral Program of Higher Education of China(Grant No.200803350031)+2 种基金Zhejiang Provincial Natural Science Foundation of China(Grant No.Y1080358)Research Fund of Zhejiang Educational Committee of China(Grant No.Y200909651)PACMAN Project within the French National Research Agency
文摘Nano assembly and manipulation technologies are the basis for nano-electro-mechanical systems（NEMS）. Atomic force microscope（AFM） is widely used to manipulate nanotubes to assemble NEMS. Manipulating nanotubes with AFM is a challenging and difficult task. One of the major reasons is the deficiency of visual information during the manipulation process. To address these difficulties, this research aims to put forward novel virtual tools and assembly strategies to improve the efficiency, accuracy and ease of the assembly process of NEMS. This paper begins by the discussion of the principles and implementation of a virtual nano-assembly simulator, which serves as a benchmark to test the proposed NEMS assembly techniques and virtual tools. Then, a general framework of nanotube-based NEMS assembly is proposed. Several nano-assembly strategies and virtual tools, such as automated path planning for NEMS assembly, a four-step scheme of nanotube manipulation, virtual fixtures for assembly finalization and safe manipulation, are introduced. These virtual tools and methods are experimented for justification. An assembly task of moderate complexity was performed in our virtual nano-assembly simulator with and without the help of the proposed toolkit. Experimental results suggest that the proposed methods tend to greatly enhance the efficiency and accuracy of nanotube-based NEMS assembly. In general, the proposed virtual reality toolkit not only ensures the safety, but also enhances the accuracy and efficiency of the assembly of nanotube-based NEMS.
基金supported by the National Natural Science Foundation of China (11374110, 51371085, 11304106)
文摘Nowadays, fabrication of micro/nano-scale electronic devices with bottom-up approach is paid much research attention. Here, we provide a novel micro/nano-assembling method, which is accurate and efficient, especially suitable for the fabrication of micro/nano-scale electronic devices. Using this method, a self-powered ZnO/Sb-doped ZnO nanowire p–n homojunction ultraviolet detector(UVD) was fabricated, and the detailed photoelectric properties were tested. At a reverse bias of -0.1 V under UV light illumination, the photoresponse sensitivity of the UVD was 26.5 and the rise/decay time of the UVD was as short as 30 ms. The micro/nano-assembling method has wide potential applications in the fabrication of specific micro/nano-scale electronic devices.
