Strategically coupling nanoparticle hybrids and internal thermosensitive molecular switches establishes an innovative paradigm for constructing micro/nanoscale-reconfigurable robots,facilitating energyefficient CO_(2)...Strategically coupling nanoparticle hybrids and internal thermosensitive molecular switches establishes an innovative paradigm for constructing micro/nanoscale-reconfigurable robots,facilitating energyefficient CO_(2) management in life-support systems of confined space.Here,a micro/nano-reconfigurable robot is constructed from the CO_(2) molecular hunters,temperature-sensitive molecular switch,solar photothermal conversion,and magnetically-driven function engines.The molecular hunters within the molecular extension state can capture 6.19 mmol g^(−1) of CO_(2) to form carbamic acid and ammonium bicarbonate.Interestingly,the molecular switch of the robot activates a molecular curling state that facilitates CO_(2) release through nano-reconfiguration,which is mediated by the temperature-sensitive curling of Pluronic F127 molecular chains during the photothermal desorption.Nano-reconfiguration of robot alters the amino microenvironment,including increasing surface electrostatic potential of the amino group and decreasing overall lowest unoccupied molecular orbital energy level.This weakened the nucleophilic attack ability of the amino group toward the adsorption product derivatives,thereby inhibiting the side reactions that generate hard-to-decompose urea structures,achieving the lowest regeneration temperature of 55℃ reported to date.The engine of the robot possesses non-contact magnetically-driven micro-reconfiguration capability to achieve efficient photothermal regeneration while avoiding local overheating.Notably,the robot successfully prolonged the survival time of mice in the sealed container by up to 54.61%,effectively addressing the issue of carbon suffocation in confined spaces.This work significantly enhances life-support systems for deep-space exploration,while stimulating innovations in sustainable carbon management technologies for terrestrial extreme environments.展开更多
针对香菇育种过程中表型性状考察困难、人工测量费时费力的问题,提出一种适用于Jetson Orin Nano平台部署的香菇菌棒表型测量方法。使用手机和工业相机对3种不同数据来源的香菇菌棒进行图像采集,标注香菇菌落数据集并进行增强;比较了Bis...针对香菇育种过程中表型性状考察困难、人工测量费时费力的问题,提出一种适用于Jetson Orin Nano平台部署的香菇菌棒表型测量方法。使用手机和工业相机对3种不同数据来源的香菇菌棒进行图像采集,标注香菇菌落数据集并进行增强;比较了Bisenet、Stdcseg、U-net、Deeplabv3p、PP-liteseg 5个分割模型在Test-A、Test-B和Test-C测试集上的分割效果,结果表明PP-liteseg的普适性优于其他网络,PP-liteseg分割模型在3个测试集上的平均交并比超过97.53%,平均像素准确率高于99.49%,推理单幅图像耗时660 ms;为进一步平衡模型准确性和实时性,采用量化蒸馏方式对PP-liteseg模型进行压缩,并部署到Jetson Orin Nano平台上,压缩后的模型在Test-B测试集上的平均交并比和平均像素准确率分别为97.50%和99.51%,推理单幅图像耗时43.63 ms,比压缩前缩短近64%;采用PP-liteseg分割香菇菌棒图像,提取菌落表型,再根据菌落径向长度和轴向宽度得到菌丝生长长度,与人工测量值相比,菌丝生长长度平均绝对百分比误差、均方根误差和决定系数分别为1.874%、0.148 cm、0.918;采用该方法测量了6个菌株在连续4 d内的菌丝生长长度,结果表明,6个菌株的菌丝生长速度无论在单日还是在整个培养期内均有差异,其中49号和168号菌株差异最大。研究表明,本文方法适用于香菇菌棒表型测量,并能够以良好的准确性和实时性在Jetson Orin Nano平台上运行。展开更多
Micro/nano hierarchical structures could endow materials with various surface functions.However,the multilayer and multiscale characteristics of micro/nano hierarchical structures bring difficulties for their one step...Micro/nano hierarchical structures could endow materials with various surface functions.However,the multilayer and multiscale characteristics of micro/nano hierarchical structures bring difficulties for their one step and controllable fabrication.Accordingly,based on tip-based fabrication techniques,this study proposed a micro-amplitude vibration-assisted scratching method by introducing a periodic backward displacement into the conventional scratching process,which enabled the synchronous creation of the microscale V-groove and nanoscale ripples,i.e.a typical micro/nano hierarchical structure.The experiments and finite element modeling were employed to explore the formation process and mechanism of the micro/nano hierarchical structures.Being different from conventional cutting,this method was mainly based on the plow mechanism,and it could accurately replicate the shape of the indenter on the material surface.The microscale V-groove was formed due to the scratching action,and the nanoscale ripple was formed due to the extrusion action of the indenter on the microscale V-groove’s surface.Furthermore,the relationships between the processing parameters and the dimensions of the micro/nano hierarchical structures were established through experiments,and optimized processing parameters were determined to achieve regular micro/nano hierarchical structures.By this method,complex patterns constructed by various micro/nano hierarchical structures were fabricated on both flat and curved surfaces,achieving diverse surface structural colors.展开更多
Acute lung injury(ALI)is a critical respiratory disorder with a high mortality rate and is caused by several factors.Addressing oxidative stress and infiammation is a pivotal strategy for ALI treatment.In this study,w...Acute lung injury(ALI)is a critical respiratory disorder with a high mortality rate and is caused by several factors.Addressing oxidative stress and infiammation is a pivotal strategy for ALI treatment.In this study,we introduced a novel nanotherapeutic approach involving a curcumin-loaded ceria nanoenzyme delivery system tailored to counteract the multifaceted aspects of ALI.This system leverages the individual and combined effects of the components to provide a comprehensive therapeutic solution.The dual-action capability of this nanosystem was manifested by mitigating mitochondrial oxidative stress in lung epithelial cells and inhibiting the transient receptor potential melanosome-associated protein 2(TRPM2)-NOD-like receptor thermal protein domain associated protein 3(NLRP3)signaling pathway,offering a highly effective therapeutic approach to ALI.Our findings reveal the underlying mechanisms of this innovative nanodelivery system,showcasing its potential as a versatile strategy for ALI treatment and encouraging further exploration of nanoenzyme-based therapies for ALI.展开更多
Under the background of the new era,people’s research on nanomaterials continues to deepen,and new nanomaterials continue to develop,which plays an important role and value in many fields.Especially in the field of m...Under the background of the new era,people’s research on nanomaterials continues to deepen,and new nanomaterials continue to develop,which plays an important role and value in many fields.Especially in the field of medicine,nano-new materials have shown great value and potential in the field of biomedicine due to their unique chemical and physical properties,which promote the occurrence of many changes.This article will start with the types of new nano materials,and gradually go into their applications in the field of medical biology.Finally,it will briefly analyze the current problems of new nano materials applied in the field of biomedicine and put forward relevant solutions.It will further show the important scientific significance and wide application prospects of new nano materials,in order to contribute to the development of medicine in the future.This paper will provide a meaningful reference for other researchers.展开更多
Nanofibrous membrane has great advantages in many fields,of which the microstructural analysis and optimization are the key to the industrial application.The U-Net multiclassifier based on network structure together w...Nanofibrous membrane has great advantages in many fields,of which the microstructural analysis and optimization are the key to the industrial application.The U-Net multiclassifier based on network structure together with the Jaccard-Lovasz extension loss function was proposed to classify the pixels of the nanofiber SEM image into three categories.A Conditional Random Field(CRF)network was utilized to post-process the segmentation results.Porosities of the filter membranes and the radii of the nanofibers were calculated based on the segmentation results.Experimental results show that the proposed U-Net multiclassifier can be used to deal with overlapped nanofibers and the corresponding segmentation results can retain important details of the SEM image.The technique is beneficial to the subsequent numerical simulation,which is of great academic and practical significance for the subsequent film performance improvement and application promotion.展开更多
Nanotechnology is gaining tremendous impetus due to its capability of modulating metals into their nanosize, which drastically changes the chemical, physical and optical properties of metals. Nanoparticles have been i...Nanotechnology is gaining tremendous impetus due to its capability of modulating metals into their nanosize, which drastically changes the chemical, physical and optical properties of metals. Nanoparticles have been intro- duced as materials with good potential to be extensively used in biological and medical applications. Nanoparticles are clusters of atoms in the size range of 1-100 nm. Inorganic nanoparticles and their nano-composites are applied as good antibacterial agents. Due to the outbreak of infectious diseases caused by different pathogenic bacteria and the development of antibiotic resistance, pharmaceutical companies and researchers are searching for new antibacterial agents. The metallic nanoparticles are the most promising as they show good antibacterial properties due to their large surface area to volume ratios, which draw growing interest from researchers due to increasing microbial resistance against metal ions, antibiotics and the development of resistant strains. Metallic nanoparticles can be used as effective growth inhibitors in various microorganisms and thereby are applicable to diverse medical devices. Nanotechnology discloses the use of elemental nanoparticles as active antibacterial ingredient for dental materials. In dentistry, both restorative materials and oral bacteria are believed to be responsible for restoration failure. Secondary caries is found to be the main reason to restoration failure. Secondary caries is primarily caused by invasion of plaque bacteria （acid-producing bacteria） such as Streptococcus mutans and lactobacilli in the presence of fermentable carbohydrates. To make long-lasting restorations, antibacterial materials should be made. The potential of nanoparticles to control the formation of biofilms within the oral cavity is also coming under in- creasing scrutiny. Possible uses of nanoparticles as topically applied agents within dental materials and the appli- cation of nanoparticles in the control of oral infections are also reviewed.展开更多
The earthworm-based vermiremediation facilitated with benign chemicals such as nano zero-valent iron(nZVI)is a promising approach for the remediation of a variety of soil contaminants including cyanotoxins.As themost ...The earthworm-based vermiremediation facilitated with benign chemicals such as nano zero-valent iron(nZVI)is a promising approach for the remediation of a variety of soil contaminants including cyanotoxins.As themost toxic cyanotoxin,microcystin-LR(MC-LR)enter soil via runoff,irrigated surface water and sewage,and the application of cyanobacterial biofertilizers as part of the sustainable agricultural practice.Earthworms in such remediation systems must sustain the potential risk from both nZVI and MC-LR.In the present study,earthworms(Eisenia fetida)were exposed up to 14 days to MC-LR and nZVI(individually and inmixture),and the toxicity was investigated at both the organismal andmetabolic levels,including growth,tissue damage,oxidative stress,metabolic response and gut microbiota.Results showed that co-exposure of MC-LR and nZVI is less potent to earthworms than that of separate exposure.Histological observations in the co-exposure group revealed only minor epidermal brokenness,and KEGG enrichment analysis showed that co-exposure induced earthworms to regulate glutathione biosynthesis for detoxification and reduced adverse effects from MC-LR.The combined use of nZVI promoted the growth and reproduction of soil and earthworm gut bacteria(e.g.,Sphingobacterium and Acinetobacter)responsible for the degradation of MC-LR,whichmight explain the observed antagonism between nZVI and MC-LR in earthworm microcosm.Our study suggests the beneficial use of nZVI to detoxify pollutants in earthworm-based vermiremediation systems where freshwater containing cyanobacterial blooms is frequently used to irrigate soil and supply water for the growth and metabolism of earthworms.展开更多
Infectious bone defects represent a substantial challenge in clinical practice,necessitating the deployment of advanced therapeutic strategies.This study presents a treatment modality that merges a mild photothermal t...Infectious bone defects represent a substantial challenge in clinical practice,necessitating the deployment of advanced therapeutic strategies.This study presents a treatment modality that merges a mild photothermal therapy hydrogel with a pulsed drug delivery mechanism.The system is predicated on a hydrogel matrix that is thermally responsive,characteristic of bone defect sites,facilitating controlled and site-specific drug release.The cornerstone of this system is the incorporation of mild photothermal nanoparticles,which are activated within the temperature range of 40–43°C,thereby enhancing the precision and efficacy of drug delivery.Our findings demonstrate that the photothermal response significantly augments the localized delivery of therapeutic agents,mitigating systemic side effects and bolstering efficacy at the defect site.The synchronized pulsed release,cooperated with mild photothermal therapy,effectively addresses infection control,and promotes bone regeneration.This approach signifies a considerable advancement in the management of infectious bone defects,offering an effective and patient-centric alternative to traditional methods.Our research endeavors to extend its applicability to a wider spectrum of tissue regeneration scenarios,underscoring its transformative potential in the realm of regenerative medicine.展开更多
基金supported by the National Natural Science Foundation of China(22168008,22378085)the Guangxi Natural Science Foundation(2024GXNSFDA010053)+1 种基金the Technology Development Project of Guangxi Bossco Environmental Protection Technology Co.,Ltd(202100039)Innovation Project of Guangxi Graduate Education(YCBZ2024065).
文摘Strategically coupling nanoparticle hybrids and internal thermosensitive molecular switches establishes an innovative paradigm for constructing micro/nanoscale-reconfigurable robots,facilitating energyefficient CO_(2) management in life-support systems of confined space.Here,a micro/nano-reconfigurable robot is constructed from the CO_(2) molecular hunters,temperature-sensitive molecular switch,solar photothermal conversion,and magnetically-driven function engines.The molecular hunters within the molecular extension state can capture 6.19 mmol g^(−1) of CO_(2) to form carbamic acid and ammonium bicarbonate.Interestingly,the molecular switch of the robot activates a molecular curling state that facilitates CO_(2) release through nano-reconfiguration,which is mediated by the temperature-sensitive curling of Pluronic F127 molecular chains during the photothermal desorption.Nano-reconfiguration of robot alters the amino microenvironment,including increasing surface electrostatic potential of the amino group and decreasing overall lowest unoccupied molecular orbital energy level.This weakened the nucleophilic attack ability of the amino group toward the adsorption product derivatives,thereby inhibiting the side reactions that generate hard-to-decompose urea structures,achieving the lowest regeneration temperature of 55℃ reported to date.The engine of the robot possesses non-contact magnetically-driven micro-reconfiguration capability to achieve efficient photothermal regeneration while avoiding local overheating.Notably,the robot successfully prolonged the survival time of mice in the sealed container by up to 54.61%,effectively addressing the issue of carbon suffocation in confined spaces.This work significantly enhances life-support systems for deep-space exploration,while stimulating innovations in sustainable carbon management technologies for terrestrial extreme environments.
文摘针对香菇育种过程中表型性状考察困难、人工测量费时费力的问题,提出一种适用于Jetson Orin Nano平台部署的香菇菌棒表型测量方法。使用手机和工业相机对3种不同数据来源的香菇菌棒进行图像采集,标注香菇菌落数据集并进行增强;比较了Bisenet、Stdcseg、U-net、Deeplabv3p、PP-liteseg 5个分割模型在Test-A、Test-B和Test-C测试集上的分割效果,结果表明PP-liteseg的普适性优于其他网络,PP-liteseg分割模型在3个测试集上的平均交并比超过97.53%,平均像素准确率高于99.49%,推理单幅图像耗时660 ms;为进一步平衡模型准确性和实时性,采用量化蒸馏方式对PP-liteseg模型进行压缩,并部署到Jetson Orin Nano平台上,压缩后的模型在Test-B测试集上的平均交并比和平均像素准确率分别为97.50%和99.51%,推理单幅图像耗时43.63 ms,比压缩前缩短近64%;采用PP-liteseg分割香菇菌棒图像,提取菌落表型,再根据菌落径向长度和轴向宽度得到菌丝生长长度,与人工测量值相比,菌丝生长长度平均绝对百分比误差、均方根误差和决定系数分别为1.874%、0.148 cm、0.918;采用该方法测量了6个菌株在连续4 d内的菌丝生长长度,结果表明,6个菌株的菌丝生长速度无论在单日还是在整个培养期内均有差异,其中49号和168号菌株差异最大。研究表明,本文方法适用于香菇菌棒表型测量,并能够以良好的准确性和实时性在Jetson Orin Nano平台上运行。
基金supported by the Jilin Province Key Research and Development Plan Project(20240302066GX)the National Natural Science Foundation of China(Grant No.52075221)the Fundamental Research Funds for the Central Universities(2023-JCXK-02)。
文摘Micro/nano hierarchical structures could endow materials with various surface functions.However,the multilayer and multiscale characteristics of micro/nano hierarchical structures bring difficulties for their one step and controllable fabrication.Accordingly,based on tip-based fabrication techniques,this study proposed a micro-amplitude vibration-assisted scratching method by introducing a periodic backward displacement into the conventional scratching process,which enabled the synchronous creation of the microscale V-groove and nanoscale ripples,i.e.a typical micro/nano hierarchical structure.The experiments and finite element modeling were employed to explore the formation process and mechanism of the micro/nano hierarchical structures.Being different from conventional cutting,this method was mainly based on the plow mechanism,and it could accurately replicate the shape of the indenter on the material surface.The microscale V-groove was formed due to the scratching action,and the nanoscale ripple was formed due to the extrusion action of the indenter on the microscale V-groove’s surface.Furthermore,the relationships between the processing parameters and the dimensions of the micro/nano hierarchical structures were established through experiments,and optimized processing parameters were determined to achieve regular micro/nano hierarchical structures.By this method,complex patterns constructed by various micro/nano hierarchical structures were fabricated on both flat and curved surfaces,achieving diverse surface structural colors.
基金funded by the National Natural Science Foundation of China(Nos.82103885,81871521,82273672)Natural Science Foundation of Shanghai(Nos.21ZR1477700,20ZR1470300)+1 种基金the Shanghai Municipal Health Commission-Outstanding Youth Foundation of Public Health(No.GWV-10.2-YQ48)Sci Tech Funding by CSPFTZ Lingang Special Area Marine Biomedical Innovation Platform。
文摘Acute lung injury(ALI)is a critical respiratory disorder with a high mortality rate and is caused by several factors.Addressing oxidative stress and infiammation is a pivotal strategy for ALI treatment.In this study,we introduced a novel nanotherapeutic approach involving a curcumin-loaded ceria nanoenzyme delivery system tailored to counteract the multifaceted aspects of ALI.This system leverages the individual and combined effects of the components to provide a comprehensive therapeutic solution.The dual-action capability of this nanosystem was manifested by mitigating mitochondrial oxidative stress in lung epithelial cells and inhibiting the transient receptor potential melanosome-associated protein 2(TRPM2)-NOD-like receptor thermal protein domain associated protein 3(NLRP3)signaling pathway,offering a highly effective therapeutic approach to ALI.Our findings reveal the underlying mechanisms of this innovative nanodelivery system,showcasing its potential as a versatile strategy for ALI treatment and encouraging further exploration of nanoenzyme-based therapies for ALI.
文摘Under the background of the new era,people’s research on nanomaterials continues to deepen,and new nanomaterials continue to develop,which plays an important role and value in many fields.Especially in the field of medicine,nano-new materials have shown great value and potential in the field of biomedicine due to their unique chemical and physical properties,which promote the occurrence of many changes.This article will start with the types of new nano materials,and gradually go into their applications in the field of medical biology.Finally,it will briefly analyze the current problems of new nano materials applied in the field of biomedicine and put forward relevant solutions.It will further show the important scientific significance and wide application prospects of new nano materials,in order to contribute to the development of medicine in the future.This paper will provide a meaningful reference for other researchers.
基金supported by the National Natural Science Foundation of China (52275575)the Development and Reform Commission of Shenzhen Municipality (JSGG20220831094600002)Natural Science Foundation of Guangdong Province (2022A1515010923, 2022A1515010949)。
文摘Nanofibrous membrane has great advantages in many fields,of which the microstructural analysis and optimization are the key to the industrial application.The U-Net multiclassifier based on network structure together with the Jaccard-Lovasz extension loss function was proposed to classify the pixels of the nanofiber SEM image into three categories.A Conditional Random Field(CRF)network was utilized to post-process the segmentation results.Porosities of the filter membranes and the radii of the nanofibers were calculated based on the segmentation results.Experimental results show that the proposed U-Net multiclassifier can be used to deal with overlapped nanofibers and the corresponding segmentation results can retain important details of the SEM image.The technique is beneficial to the subsequent numerical simulation,which is of great academic and practical significance for the subsequent film performance improvement and application promotion.
文摘Nanotechnology is gaining tremendous impetus due to its capability of modulating metals into their nanosize, which drastically changes the chemical, physical and optical properties of metals. Nanoparticles have been intro- duced as materials with good potential to be extensively used in biological and medical applications. Nanoparticles are clusters of atoms in the size range of 1-100 nm. Inorganic nanoparticles and their nano-composites are applied as good antibacterial agents. Due to the outbreak of infectious diseases caused by different pathogenic bacteria and the development of antibiotic resistance, pharmaceutical companies and researchers are searching for new antibacterial agents. The metallic nanoparticles are the most promising as they show good antibacterial properties due to their large surface area to volume ratios, which draw growing interest from researchers due to increasing microbial resistance against metal ions, antibiotics and the development of resistant strains. Metallic nanoparticles can be used as effective growth inhibitors in various microorganisms and thereby are applicable to diverse medical devices. Nanotechnology discloses the use of elemental nanoparticles as active antibacterial ingredient for dental materials. In dentistry, both restorative materials and oral bacteria are believed to be responsible for restoration failure. Secondary caries is found to be the main reason to restoration failure. Secondary caries is primarily caused by invasion of plaque bacteria （acid-producing bacteria） such as Streptococcus mutans and lactobacilli in the presence of fermentable carbohydrates. To make long-lasting restorations, antibacterial materials should be made. The potential of nanoparticles to control the formation of biofilms within the oral cavity is also coming under in- creasing scrutiny. Possible uses of nanoparticles as topically applied agents within dental materials and the appli- cation of nanoparticles in the control of oral infections are also reviewed.
基金supported by the National Natural Science Foundation of China(No.21777139)the National Key Research and Development Program of China(No.2017YFA0207003).
文摘The earthworm-based vermiremediation facilitated with benign chemicals such as nano zero-valent iron(nZVI)is a promising approach for the remediation of a variety of soil contaminants including cyanotoxins.As themost toxic cyanotoxin,microcystin-LR(MC-LR)enter soil via runoff,irrigated surface water and sewage,and the application of cyanobacterial biofertilizers as part of the sustainable agricultural practice.Earthworms in such remediation systems must sustain the potential risk from both nZVI and MC-LR.In the present study,earthworms(Eisenia fetida)were exposed up to 14 days to MC-LR and nZVI(individually and inmixture),and the toxicity was investigated at both the organismal andmetabolic levels,including growth,tissue damage,oxidative stress,metabolic response and gut microbiota.Results showed that co-exposure of MC-LR and nZVI is less potent to earthworms than that of separate exposure.Histological observations in the co-exposure group revealed only minor epidermal brokenness,and KEGG enrichment analysis showed that co-exposure induced earthworms to regulate glutathione biosynthesis for detoxification and reduced adverse effects from MC-LR.The combined use of nZVI promoted the growth and reproduction of soil and earthworm gut bacteria(e.g.,Sphingobacterium and Acinetobacter)responsible for the degradation of MC-LR,whichmight explain the observed antagonism between nZVI and MC-LR in earthworm microcosm.Our study suggests the beneficial use of nZVI to detoxify pollutants in earthworm-based vermiremediation systems where freshwater containing cyanobacterial blooms is frequently used to irrigate soil and supply water for the growth and metabolism of earthworms.
基金supported by the National Natural Science Foundation of China(32171354,82222015,82171001)The National Key Research and Development Program of China2023YFC2413600Research Funding from West China School/Hospital of Stomatology,Sichuan University(No.RCDWIS2023-1).
文摘Infectious bone defects represent a substantial challenge in clinical practice,necessitating the deployment of advanced therapeutic strategies.This study presents a treatment modality that merges a mild photothermal therapy hydrogel with a pulsed drug delivery mechanism.The system is predicated on a hydrogel matrix that is thermally responsive,characteristic of bone defect sites,facilitating controlled and site-specific drug release.The cornerstone of this system is the incorporation of mild photothermal nanoparticles,which are activated within the temperature range of 40–43°C,thereby enhancing the precision and efficacy of drug delivery.Our findings demonstrate that the photothermal response significantly augments the localized delivery of therapeutic agents,mitigating systemic side effects and bolstering efficacy at the defect site.The synchronized pulsed release,cooperated with mild photothermal therapy,effectively addresses infection control,and promotes bone regeneration.This approach signifies a considerable advancement in the management of infectious bone defects,offering an effective and patient-centric alternative to traditional methods.Our research endeavors to extend its applicability to a wider spectrum of tissue regeneration scenarios,underscoring its transformative potential in the realm of regenerative medicine.
