[Objective]To study the seeds germination of Lycium ruthenicum Murr.under different concentrations of NaCl,as well as to find the optimal concentration of NaCl for the germination of L.ruthenicum.[Method]The seeds of ...[Objective]To study the seeds germination of Lycium ruthenicum Murr.under different concentrations of NaCl,as well as to find the optimal concentration of NaCl for the germination of L.ruthenicum.[Method]The seeds of L.ruthenicum were treated with different concentrations of NaCl,and the state of seed germination was measured.[Result]With the increasing of concentration of NaCl,the seed germination rate of L.ruthenicum showed an obvious increasing trend.when the concentration was of 0.3%-0.4 %,the germination rate was the highest,and when the concentration of NaCl was greater than 0.4,the germination rate showed a decline trend.[Conclusion]After treated with appropriate concentrations of NaCl before sowing,the germination rate of seeds of L.ruthenicum would increase.展开更多
The effects of nitrogen on physiological and biochemical characteristics of potato cultivar Zihuabai under NaCl stress were studied in culture condition.The results show that,under NaCl stress,the contents of proline,...The effects of nitrogen on physiological and biochemical characteristics of potato cultivar Zihuabai under NaCl stress were studied in culture condition.The results show that,under NaCl stress,the contents of proline,chlorophyll and protein and root system vitality first increase and then decrease with the increase of nitrogen level,and reach the top under 4.17 mmol/L NH4NO3 level.Wherein,the contents of chlorophyll,protein and root system vitality are respectively 69.88%,13.07% and 59.29% higher than that of the control under 4.17 mmol/L NH4NO3 level;the activities of superoxide dismutase（SOD）and peroxidase（POD）increase generally under NaCl stress with the increase of nitrogen level,and reach the peaks [111.83 U/g and 25.467 U/（g·min）],which are 37.73% and 35.46% higher than that of control,at 6.25 mmol/L NH4NO3 level.展开更多
[ Objective ] Study on the changes of chlorophyll fluorescence parameters in Cinnamomumjaponicum var. chenii under NaCl stress. [ Method ] The seedling growth increment, chlorophyll content and chlorophyll fluorescenc...[ Objective ] Study on the changes of chlorophyll fluorescence parameters in Cinnamomumjaponicum var. chenii under NaCl stress. [ Method ] The seedling growth increment, chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [ Result] Under NaC1 stress, seedling growth increment reduced and the chlorophyll content decreased to a stable value ; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress, most variations were observed in Fo, correlations among chlorophyll fluorescence parameters presented ＂rise-drop＂ trend （in.the treatment of 7 g/L NaCl）. [ Conclusion] Cirmamomum japonicum vat. chenii is endowed with strong salt resistance and wide adaptability.展开更多
Seedlings of six Glycine max cultivars were used to compare Cl- with Na+ in stress effects on soybean seedlings under NaCl stress. Results showed that stress of NaCl on G. max seedlings was mainly caused by Cl- and no...Seedlings of six Glycine max cultivars were used to compare Cl- with Na+ in stress effects on soybean seedlings under NaCl stress. Results showed that stress of NaCl on G. max seedlings was mainly caused by Cl- and not by Na+. After treatment with isoosmotic solutions of Cl- (without Na+) and Na+ (without Cl-) respectively, fresh weight and height of G. max seedlings growing in solution of Cl- were much more stressed than those in solution of Na+. And the roots and leaves electrolyte leakage were much more increased under stress of Cl- than those under stress of Na+. Salt tolerance of G. max seedlings was mainly contributed to Cl- withheld in roots of seedlings to decrease its content in leaves.展开更多
[ Objective ] The paper was to study effects of different salt treatments on growth and physiological activity of sunflower seedlings, in order to provide a theoretical basis for sunflower cultivation in saline soil. ...[ Objective ] The paper was to study effects of different salt treatments on growth and physiological activity of sunflower seedlings, in order to provide a theoretical basis for sunflower cultivation in saline soil. [Method] With 567DW sunflower as the material, effects of different concentrations of NaC1 （0, 0.06, 0. 12, 0. 18, 0.24 mol/L） on growth and physiological activity of sunflower seedlings were studied. [ Result] With the increasing concentration of NaCl, the morphological indexes of sunflower decreased significantly; MDA content increased, while Pro content and POD activity first increased and then decreased. Under the treatment of 0. 12 mol/L, Pro content and POD activity reached the maximum value, respectively. [ Conclusion] The critical salt-tolerance concentration of sunflower was about 0.12 mol/L.展开更多
In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We ...In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.展开更多
The solution culture method was used to study the effect of increasing nitrogen on the growth and pho-tosynthesis of poplar seedlings under 100 mmol L-1 NaCl stress. I Increase in nitrogen reduced stomatal limitation ...The solution culture method was used to study the effect of increasing nitrogen on the growth and pho-tosynthesis of poplar seedlings under 100 mmol L-1 NaCl stress. I Increase in nitrogen reduced stomatal limitation of leaves under NaCl stress, improved utilization of CO2 by mesophyll cells, enhanced photosynthetic carbon assimi-lation capacity, significantly alleviated saline damage of NaCl, and promoted the accumulation of aboveground and root biomass. I Increased nitrogen enhanced photochemical efficiency (ΦPSⅡ) and electron transport rates, relieved the reduction of maximum photochemical efficiency (Fv/Fm) under NaCl, and reduced the degree of photoinhibition caused by NaCl stress. Increased nitrogen applications reduced the proportion of energy dissipating in the form of ineffective heat energy and hence a greater proportion of light energy absorbed by leaves was allocated to photo-chemical reactions. Under treatment with increased nitro-gen, the synergistic effect of heat dissipation and the xanthophyll cycle in the leaves effectively protected pho-tosynthetic PSⅡ and enhanced light energy utilization of leaves under NaCl stress. The increased nitrogen promoted photosynthetic electron supply and transport ability under NaCl stress evident in enhanced functioning of the oxygen-evolving complex on the electron donor side of PS Ⅱ. It increased the ability of the receptor pool to accept electrons on the PSII electron acceptor side and improved the sta-bility of thylakoid membranes under NaCl stress. Therefore, increasing nitrogen applications under NaCl stress can promote poplar growth by improving the effi-ciency of light energy utilization.展开更多
In order to explore the salt-tolerance of Narcissus pseudonarcissus and enrich its cultivation and application forms. In this experiment, “Manly”, “Dutch” and “Wilden” daffodils were used as test materials. NaCl...In order to explore the salt-tolerance of Narcissus pseudonarcissus and enrich its cultivation and application forms. In this experiment, “Manly”, “Dutch” and “Wilden” daffodils were used as test materials. NaCl stress was applied at different concentrations, and the corresponding physiological indexes were measured at three time nodes: bolting stage, early flowering stage and blooming stage. The results showed that all three showed some resistance under low concentration salt stress, in which, SOD, POD enzyme activity, chlorophyll proline and soluble sugar content increased, while MDA content decreased. The physiological metabolism of the three was disturbed under high concentration of salt stress, in which, SOD, POD enzyme activity and chlorophyll content decreased, while MDA, proline and soluble sugar content increased. With the extension of stress time, the injury was gradually deepened and the salt tolerance of the three varieties is “Dutch”, “Wilden” and “Manly” in order.展开更多
Traditional detection methods of crop information are often destructive and low efficiency.In this study,a new evaluation method was developed based on photosynthesis and chlorophyll fluorescence.Via analysis of the c...Traditional detection methods of crop information are often destructive and low efficiency.In this study,a new evaluation method was developed based on photosynthesis and chlorophyll fluorescence.Via analysis of the changes under NaCl stress during the seedling stage of two varieties,the salt resistance mechanism of soybeans was explored and a non-destructive stress-recognition method was developed.In this experiment,two soybean varieties were treated with one of four levels of NaCl stress:CK(0 mmol/L),LS(50 mmol/L),MS(100 mmol/L),and HS(150 mmol/L),for 15 d.The normal functions of the photosynthetic system of soybeans were enhanced under LS NaCl stress,but were inhibited under HS NaCl stress.Biomass,net leaf photosynthetic rate(Pn),stomatal conductance(gs),intercellular carbon dioxide concentration(Ci),transpiration rate(Tr),chlorophyll fluorescence parameters Y(II)and PSII decreased.However,in contrast to the findings of other studies on the influence of severe drought stress on soybean for long periods in which non-photochemical quenching coefficient(qN)decreased,this parameter increased under salt stress in soybean.The results demonstrate that the method developed is a promising tool for rapid and non-destructive detection of soybean photosynthetic responses under salt stress in the field.展开更多
Dopamine can be used to treat depression,myocardial infarction,and other diseases.However,few reports are available on the de novo microbial synthesis of dopamine from low-cost substrate.In this study,integrated omics...Dopamine can be used to treat depression,myocardial infarction,and other diseases.However,few reports are available on the de novo microbial synthesis of dopamine from low-cost substrate.In this study,integrated omics technology was used to explore the dopamine metabolism of a novel marine multi-stress-tolerant aromatic yeast Meyerozyma guilliermondii GXDK6.GXDK6 was found to have the ability to biosynthesize dopamine when using glucose as the substrate.14 key genes for the biosynthesis of dopamine were identified by whole genome-wide analysis.Transcriptomic and proteomic data showed that the expression levels of gene AAT2 encoding aspartate aminotransferase(regulating dopamine anabolism)were upregulated,while gene AO-I encoding copper amine oxidase(involved in dopamine catabolism)were downregulated under 10%NaCl stress compared with non-NaCl stress,thereby contributing to biosynthesis of dopamine.Further,the amount of dopamine under 10%NaCl stress was 2.51-fold higher than that of zero NaCl,which was consistent with the multi-omics results.Real-time fluorescence quantitative PCR(RT-qPCR)and high-performance liquid chromatography(HPLC)results confirmed the metabolic model of dopamine.Furthermore,by overexpressing AAT2,AST enzyme activity was increased by 24.89%,the expression of genes related to dopamine metabolism was enhanced,and dopamine production was increased by 56.36%in recombinant GXDK6AAT2.In conclusion,Meyerozyma guilliermondii GXDK6 could utilize low-cost carbon source to synthesize dopamine,and NaCl stress promoted the biosynthesis of dopamine.展开更多
Both enhanced were H^+ transport activities of tonoplast vesicles isolated from roots of the salt-tolerant mutant and wild type of wheat with treatment of NaCI, but the activity of the mutant was significantly higher ...Both enhanced were H^+ transport activities of tonoplast vesicles isolated from roots of the salt-tolerant mutant and wild type of wheat with treatment of NaCI, but the activity of the mutant was significantly higher than that of wild type. H^+ transport activity was indicated as the stable value of fluorescence quenching per mg membrane proteins. The H^+ transport activities dependent on ATP of the mutant and wild type were 1099 and 558 respectively and their activities dependent on PPi were 358 and 228 separately.展开更多
文摘[Objective]To study the seeds germination of Lycium ruthenicum Murr.under different concentrations of NaCl,as well as to find the optimal concentration of NaCl for the germination of L.ruthenicum.[Method]The seeds of L.ruthenicum were treated with different concentrations of NaCl,and the state of seed germination was measured.[Result]With the increasing of concentration of NaCl,the seed germination rate of L.ruthenicum showed an obvious increasing trend.when the concentration was of 0.3%-0.4 %,the germination rate was the highest,and when the concentration of NaCl was greater than 0.4,the germination rate showed a decline trend.[Conclusion]After treated with appropriate concentrations of NaCl before sowing,the germination rate of seeds of L.ruthenicum would increase.
基金Supported by National Key Technology Research and Development Program(2007BAD49B03-1)~~
文摘The effects of nitrogen on physiological and biochemical characteristics of potato cultivar Zihuabai under NaCl stress were studied in culture condition.The results show that,under NaCl stress,the contents of proline,chlorophyll and protein and root system vitality first increase and then decrease with the increase of nitrogen level,and reach the top under 4.17 mmol/L NH4NO3 level.Wherein,the contents of chlorophyll,protein and root system vitality are respectively 69.88%,13.07% and 59.29% higher than that of the control under 4.17 mmol/L NH4NO3 level;the activities of superoxide dismutase（SOD）and peroxidase（POD）increase generally under NaCl stress with the increase of nitrogen level,and reach the peaks [111.83 U/g and 25.467 U/（g·min）],which are 37.73% and 35.46% higher than that of control,at 6.25 mmol/L NH4NO3 level.
基金Key Scientific Research Project of Zhejiang Province (2005G12004)~~
文摘[ Objective ] Study on the changes of chlorophyll fluorescence parameters in Cinnamomumjaponicum var. chenii under NaCl stress. [ Method ] The seedling growth increment, chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [ Result] Under NaC1 stress, seedling growth increment reduced and the chlorophyll content decreased to a stable value ; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress, most variations were observed in Fo, correlations among chlorophyll fluorescence parameters presented ＂rise-drop＂ trend （in.the treatment of 7 g/L NaCl）. [ Conclusion] Cirmamomum japonicum vat. chenii is endowed with strong salt resistance and wide adaptability.
基金the Scientific Foundation Special for Ph D Programs in Chinese Universities (1999002005) National Natural Science Foundation of China (39870069) the International Foundation for Science (C/3143-1), China.
文摘Seedlings of six Glycine max cultivars were used to compare Cl- with Na+ in stress effects on soybean seedlings under NaCl stress. Results showed that stress of NaCl on G. max seedlings was mainly caused by Cl- and not by Na+. After treatment with isoosmotic solutions of Cl- (without Na+) and Na+ (without Cl-) respectively, fresh weight and height of G. max seedlings growing in solution of Cl- were much more stressed than those in solution of Na+. And the roots and leaves electrolyte leakage were much more increased under stress of Cl- than those under stress of Na+. Salt tolerance of G. max seedlings was mainly contributed to Cl- withheld in roots of seedlings to decrease its content in leaves.
基金Supported by National Natural Science Funds for Distinguished Young Scholar"Effect of Se and Its Accumulation on Sunflower in the Saline-alkali"(31301851)
文摘[ Objective ] The paper was to study effects of different salt treatments on growth and physiological activity of sunflower seedlings, in order to provide a theoretical basis for sunflower cultivation in saline soil. [Method] With 567DW sunflower as the material, effects of different concentrations of NaC1 （0, 0.06, 0. 12, 0. 18, 0.24 mol/L） on growth and physiological activity of sunflower seedlings were studied. [ Result] With the increasing concentration of NaCl, the morphological indexes of sunflower decreased significantly; MDA content increased, while Pro content and POD activity first increased and then decreased. Under the treatment of 0. 12 mol/L, Pro content and POD activity reached the maximum value, respectively. [ Conclusion] The critical salt-tolerance concentration of sunflower was about 0.12 mol/L.
基金Supported by the Commonweal Item of State Oceanic Administration of the People’s Republic of China(No.201505033)the Shandong Province Key Research and Development Project(No.2017GHY215008)the Qingdao Science and Technology Project(No.17-3-3-60-nsh)
文摘In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.
基金supported by the Fundamental Research Funds for the Central Universities(2572018BE05)the National Natural Science Foundation of China(3150032331370426)
文摘The solution culture method was used to study the effect of increasing nitrogen on the growth and pho-tosynthesis of poplar seedlings under 100 mmol L-1 NaCl stress. I Increase in nitrogen reduced stomatal limitation of leaves under NaCl stress, improved utilization of CO2 by mesophyll cells, enhanced photosynthetic carbon assimi-lation capacity, significantly alleviated saline damage of NaCl, and promoted the accumulation of aboveground and root biomass. I Increased nitrogen enhanced photochemical efficiency (ΦPSⅡ) and electron transport rates, relieved the reduction of maximum photochemical efficiency (Fv/Fm) under NaCl, and reduced the degree of photoinhibition caused by NaCl stress. Increased nitrogen applications reduced the proportion of energy dissipating in the form of ineffective heat energy and hence a greater proportion of light energy absorbed by leaves was allocated to photo-chemical reactions. Under treatment with increased nitro-gen, the synergistic effect of heat dissipation and the xanthophyll cycle in the leaves effectively protected pho-tosynthetic PSⅡ and enhanced light energy utilization of leaves under NaCl stress. The increased nitrogen promoted photosynthetic electron supply and transport ability under NaCl stress evident in enhanced functioning of the oxygen-evolving complex on the electron donor side of PS Ⅱ. It increased the ability of the receptor pool to accept electrons on the PSII electron acceptor side and improved the sta-bility of thylakoid membranes under NaCl stress. Therefore, increasing nitrogen applications under NaCl stress can promote poplar growth by improving the effi-ciency of light energy utilization.
文摘In order to explore the salt-tolerance of Narcissus pseudonarcissus and enrich its cultivation and application forms. In this experiment, “Manly”, “Dutch” and “Wilden” daffodils were used as test materials. NaCl stress was applied at different concentrations, and the corresponding physiological indexes were measured at three time nodes: bolting stage, early flowering stage and blooming stage. The results showed that all three showed some resistance under low concentration salt stress, in which, SOD, POD enzyme activity, chlorophyll proline and soluble sugar content increased, while MDA content decreased. The physiological metabolism of the three was disturbed under high concentration of salt stress, in which, SOD, POD enzyme activity and chlorophyll content decreased, while MDA, proline and soluble sugar content increased. With the extension of stress time, the injury was gradually deepened and the salt tolerance of the three varieties is “Dutch”, “Wilden” and “Manly” in order.
基金This work was financially supported by the Key-Area Research and Development Program of Guangdong Province(Grant No.2019B020214005)Science and Technology Innovation Special Construction Funded Program of Beijing Academy of Agriculture and Forestry Sciences(Grant No.KJCX20170418)the National Natural Science Foundation of China(Grant No.21974012).
文摘Traditional detection methods of crop information are often destructive and low efficiency.In this study,a new evaluation method was developed based on photosynthesis and chlorophyll fluorescence.Via analysis of the changes under NaCl stress during the seedling stage of two varieties,the salt resistance mechanism of soybeans was explored and a non-destructive stress-recognition method was developed.In this experiment,two soybean varieties were treated with one of four levels of NaCl stress:CK(0 mmol/L),LS(50 mmol/L),MS(100 mmol/L),and HS(150 mmol/L),for 15 d.The normal functions of the photosynthetic system of soybeans were enhanced under LS NaCl stress,but were inhibited under HS NaCl stress.Biomass,net leaf photosynthetic rate(Pn),stomatal conductance(gs),intercellular carbon dioxide concentration(Ci),transpiration rate(Tr),chlorophyll fluorescence parameters Y(II)and PSII decreased.However,in contrast to the findings of other studies on the influence of severe drought stress on soybean for long periods in which non-photochemical quenching coefficient(qN)decreased,this parameter increased under salt stress in soybean.The results demonstrate that the method developed is a promising tool for rapid and non-destructive detection of soybean photosynthetic responses under salt stress in the field.
基金supported by the Funding Project of Chinese Central Government Guiding to the Guangxi Local Science and Technology Development(Grant No.GUIKEZY21195021)the Natural Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Region of China(Grant No.2019GXNSFFA245011)+2 种基金the Funding Project of Chinese Central Government Guiding to the Nanning Local Science and Technology Development(Grant No.20231012)the Funding Projects of Guangxi Key Research and Development Plan(GUIKE AB23075173)the Funding Project of Technological Development from Angel Yeast(Chongzuo)Co.,Ltd.(Grant No.JS1006020230722019).
文摘Dopamine can be used to treat depression,myocardial infarction,and other diseases.However,few reports are available on the de novo microbial synthesis of dopamine from low-cost substrate.In this study,integrated omics technology was used to explore the dopamine metabolism of a novel marine multi-stress-tolerant aromatic yeast Meyerozyma guilliermondii GXDK6.GXDK6 was found to have the ability to biosynthesize dopamine when using glucose as the substrate.14 key genes for the biosynthesis of dopamine were identified by whole genome-wide analysis.Transcriptomic and proteomic data showed that the expression levels of gene AAT2 encoding aspartate aminotransferase(regulating dopamine anabolism)were upregulated,while gene AO-I encoding copper amine oxidase(involved in dopamine catabolism)were downregulated under 10%NaCl stress compared with non-NaCl stress,thereby contributing to biosynthesis of dopamine.Further,the amount of dopamine under 10%NaCl stress was 2.51-fold higher than that of zero NaCl,which was consistent with the multi-omics results.Real-time fluorescence quantitative PCR(RT-qPCR)and high-performance liquid chromatography(HPLC)results confirmed the metabolic model of dopamine.Furthermore,by overexpressing AAT2,AST enzyme activity was increased by 24.89%,the expression of genes related to dopamine metabolism was enhanced,and dopamine production was increased by 56.36%in recombinant GXDK6AAT2.In conclusion,Meyerozyma guilliermondii GXDK6 could utilize low-cost carbon source to synthesize dopamine,and NaCl stress promoted the biosynthesis of dopamine.
文摘Both enhanced were H^+ transport activities of tonoplast vesicles isolated from roots of the salt-tolerant mutant and wild type of wheat with treatment of NaCI, but the activity of the mutant was significantly higher than that of wild type. H^+ transport activity was indicated as the stable value of fluorescence quenching per mg membrane proteins. The H^+ transport activities dependent on ATP of the mutant and wild type were 1099 and 558 respectively and their activities dependent on PPi were 358 and 228 separately.
