Based on the understanding of lower limb numbness after stroke in Zhuang medicine, this study makes diagnosis and treatment of lower limb numbness after stroke from the clinical differentiation and treatment ideas of ...Based on the understanding of lower limb numbness after stroke in Zhuang medicine, this study makes diagnosis and treatment of lower limb numbness after stroke from the clinical differentiation and treatment ideas of "numbness due to Shaoyang (lesser Yang)" through Zhuang Medicine Meridian Tendon Therapy. The triple therapy of meridian tendon Tuina+acupuncture+cupping originally created by Zhuang medicine and head bloodletting by Zhuang medicine were used to improve the numbness symptoms of patients. This provides a new thinking mode for acupuncture and moxibustion treatment of lower limb numbness after stroke.展开更多
Developing and identifying effective medications and targets for treating hepatic fibrosis is an urgent priority.Our previous research demonstrated the efficacy of artesunate(ART)in alleviating liver fibrosis by elimi...Developing and identifying effective medications and targets for treating hepatic fibrosis is an urgent priority.Our previous research demonstrated the efficacy of artesunate(ART)in alleviating liver fibrosis by eliminating activated hepatic stellate cells(HSCs).However,the underlying mechanism remains unclear despite these findings.Notably,endocytic adaptor protein(NUMB)has significant implications for treating hepatic diseases,but current research primarily focuses on liver regeneration and hepatocellular carcinoma.The precise function of NUMB in liver fibrosis,particularly its ability to regulate HSCs,requires further investigation.This study aims to elucidate the role of NUMB in the anti-hepatic fibrosis action of ART in HSCs.We observed that the expression level of NUMB significantly decreased in activated HSCs compared to quiescent HSCs,exhibiting a negative correlation with the progression of liver fibrosis.Additionally,ART induced senescence in activated HSCs through the NUMB/P53 tumor suppressor(P53)axis.We identified NUMB as a crucial regulator of senescence in activated HSCs and as a mediator of ART in determining cell fate.This research examines the specific target of ART in eliminating activated HSCs,providing both theoretical and experimental evidence for the treatment of liver fibrosis.展开更多
基金Supported by Key Laboratory of Virus Disease Research of Zhuang Medicine in Guangxi University of Chinese Medicine(Gui Jiao Ke Yan[2022]No.10)The Key Discipline Zhuang Medicine Meridian Tendon Tuina of Traditional Chinese Medicine in Guangxi Zhuang Medicine(GZXK-Z-20-61)+1 种基金Gui School of Traditional Chinese Medicine Master Physician Project(Gui Zhong Yi Yao Ke Jiao Fa[2022]No.6)Research and Application of Key Techniques for Prevention and Treatment of Lumbago and Leg Pain in Zhuang Medicine(Guike AB21196035).
文摘Based on the understanding of lower limb numbness after stroke in Zhuang medicine, this study makes diagnosis and treatment of lower limb numbness after stroke from the clinical differentiation and treatment ideas of "numbness due to Shaoyang (lesser Yang)" through Zhuang Medicine Meridian Tendon Therapy. The triple therapy of meridian tendon Tuina+acupuncture+cupping originally created by Zhuang medicine and head bloodletting by Zhuang medicine were used to improve the numbness symptoms of patients. This provides a new thinking mode for acupuncture and moxibustion treatment of lower limb numbness after stroke.
基金supported by the National Natural Science Foundation of China(Nos.82474164,82374124,82073914,82173874,82274185,82305046 and 82304902)the Natural Science Foundation of Jiangsu Province(Nos.BK20230458 and BK20220467)+1 种基金the General Project of the Natural Science Research of Jiangsu Higher Education Institutions(No.23KJB310017)Young Elite Scientists Sponsorship Program by CACM(No.2022-QNRC2-B15).
文摘Developing and identifying effective medications and targets for treating hepatic fibrosis is an urgent priority.Our previous research demonstrated the efficacy of artesunate(ART)in alleviating liver fibrosis by eliminating activated hepatic stellate cells(HSCs).However,the underlying mechanism remains unclear despite these findings.Notably,endocytic adaptor protein(NUMB)has significant implications for treating hepatic diseases,but current research primarily focuses on liver regeneration and hepatocellular carcinoma.The precise function of NUMB in liver fibrosis,particularly its ability to regulate HSCs,requires further investigation.This study aims to elucidate the role of NUMB in the anti-hepatic fibrosis action of ART in HSCs.We observed that the expression level of NUMB significantly decreased in activated HSCs compared to quiescent HSCs,exhibiting a negative correlation with the progression of liver fibrosis.Additionally,ART induced senescence in activated HSCs through the NUMB/P53 tumor suppressor(P53)axis.We identified NUMB as a crucial regulator of senescence in activated HSCs and as a mediator of ART in determining cell fate.This research examines the specific target of ART in eliminating activated HSCs,providing both theoretical and experimental evidence for the treatment of liver fibrosis.
文摘目的 探讨飞燕草素调节Numb/Notch信号通路诱导卵巢癌细胞A2780凋亡的作用机制。方法 使用顺铂(500μmoL/mL)和低剂量(500μmoL/mL)、高剂量(1000μmoL/mL)飞燕草素处理卵巢癌细胞A2780,同时设立卵巢癌细胞A2780对照组,各组设6个平行样,培养72 h后。实验结束后,采用CCK-8试剂盒测定各组细胞增殖水平,流式细胞术检测细胞凋亡率,Transwell法测定细胞侵袭水平,划痕实验检测细胞迁移水平,实时荧光定量(RT-qPCR)及蛋白印记法(West-blot)检测细胞Numb、Notch1 m RNA和蛋白表达水平。结果 与A2780细胞组比较,顺铂组、飞燕草素低、高剂量组A值、存活率、穿膜数、迁移距离、Notch1 m RNA和蛋白表达降低(P<0.05);与顺铂组比较,飞燕草素低剂量组A值、存活率、穿膜数、迁移距离、Notch1 m RNA和蛋白表达升高(P<0.05),高剂量组A值、存活率、穿膜数、迁移距离、Notch1 m RNA和蛋白表达无明显变化(P>0.05);与飞燕草素低剂量组比较,飞燕草素高剂量组A值、存活率、穿膜数、迁移距离、Notch1 mRNA和蛋白表达降低(P<0.05)。与A2780细胞组比较,顺铂组、飞燕草素低、高剂量组凋亡率、Numb mRNA和蛋白表达升高(P<0.05);与顺铂组比较,飞燕草素低剂量组凋亡率、Numb mRNA和蛋白表达降低(P<0.05),高剂量组凋亡率、Numb mRNA和蛋白表达无明显变化(P>0.05);与飞燕草素低剂量组比较,飞燕草素高剂量组凋亡率、Numb mRNA和蛋白表达升高(P<0.05)。结论 飞燕草素可以通过激活Numb进而抑制Notch1通路来抑制卵巢癌细胞的增殖、迁移、侵袭,促进其凋亡。
