From July 2008 to August 2008, 72 leaf samples from 22 species and 81 soil samples in the nine natural forest ecosystems were collected, from north to south along the North-South Transect of Eastern China （NSTEC）. B...From July 2008 to August 2008, 72 leaf samples from 22 species and 81 soil samples in the nine natural forest ecosystems were collected, from north to south along the North-South Transect of Eastern China （NSTEC）. Based on these samples, we studied the geographical distribution patterns of vegetable water use efficiency （WUE） and nitrogen use efficiency （NUE）, and analyzed their relationship with environmental factors. The vegetable WUE and NUE were calculated through the measurement of foliar δ 13C and C/N of predominant species, respectively. The results showed： （1） vegetable WUE, ranging from 2.13 to 28.67 mg C g-1 H2O, increased linearly from south to north in the representative forest ecosystems along the NSTEC, while vegetable NUE showed an opposite trend, increasing from north to south, ranging from 12.92 to 29.60 g C g-1 N. （2） Vegetable WUE and NUE were dominantly driven by climate and significantly affected by soil nutrient factors. Based on multiple stepwise regression analysis, mean annual temperature, soil phosphorus concentration, and soil nitrogen concentration were responding for 75.5% of the variations of WUE （p0.001）. While, mean annual precipitation and soil phosphorus concentration could explain 65.7% of the change in vegetable NUE （p0.001）. Moreover, vegetable WUE and NUE would also be seriously influenced by atmospheric nitrogen deposition in nitrogen saturated ecosystems. （3） There was a significant trade-off relationship between vegetable WUE and NUE in the typical forest ecosystems along the NSTEC （p0.001）, indicating a balanced strategy for vegetation in resource utilization in natural forest ecosystems along the NSTEC. This study suggests that global change would impact the resource use efficiency of forest ecosystems. However, vegetation could adapt to those changes by increasing the use efficiency of shortage resource while decreasing the relatively ample one. But extreme impacts, such as heavy nitrogen deposition, would break this trade-off mechanism and give a dramatic disturbance to the ecosystem biogeochemical cycle.展开更多
Nitrogen （N） application before transplanting, where N fertilizers are applied in seedling-bed and carried to the paddy field with seedlings, is a novel method proposed in this article aiming for improving nitrogen ...Nitrogen （N） application before transplanting, where N fertilizers are applied in seedling-bed and carried to the paddy field with seedlings, is a novel method proposed in this article aiming for improving nitrogen utilization efficiency （NUE） in rice. The effect of this method on mineral N distribution in the rhizosphere soil was investigated in a field experiment with a japonica variety, Ningjing 2, in seasons of 2004 and 2005. There were four levels of N applied 16 h before transplanting： zero N （NO）, 207 kg ha^-1 （NL）, 310.5 kg ha^-1 （NM）, and 414 kg ha^-1 （NH）. The result indicated that N fertilizer before transplantation had positive effect of increasing mineral N content in the rhizosphere soil of rice. Generally, N content in the rhizosphere soil of rice tended to increase with the amount of N fertilizer before transplanting, with the NH treatment having the largest effect. Additionally, N fertilizer before transplanting had significant influence on rice NUE and grain yield. Compared with other treatments, the NM treatment showed the largest influence, with basal-tillering NUE, total NUE, and grain yield being 15%, 12%, and 529.5 kg ha^-1 higher than those of NO treatment. This result indicated that N fertilizer before transplantation had positive effect on mineral N distribution in the rhizosphere soil of rice, thus improving NUE and grain yield.展开更多
Nitrogen(N)is a limiting factor that determines the yield and quality of chrysanthemum.Genetic variation in N use efficiency(NUE)has been reported among chrysanthemum genotypes.We performed a transcriptome analysis of...Nitrogen(N)is a limiting factor that determines the yield and quality of chrysanthemum.Genetic variation in N use efficiency(NUE)has been reported among chrysanthemum genotypes.We performed a transcriptome analysis of two chrysanthemum genotypes,'Nannonglihuang'(LH,N-efficient genotype)and'Nannongxuefeng"(XF,N-inefficient genotype),under low N(0.4 mmol L^(-1)N)and normal N(8 mmol L^(-1)N)treatments for 15 d and an N recovery treatment for 12 h(low N treatment for 15 d and then normal N treatment for 12 h)to understand the genetic factors impacting NUE in chrysanthemum.The two genotypes exhibited contrasting responses to the different N treatments.The N-efficient genotype LH had significant superiority in agronomic traits,N accumulation and glutamine synthase activity under both normal N and low N treatments.Low N treatment promoted root growth in LH,but inhibited root growth in XF.Transcriptome analysis revealed that the low N treatment increased the expression of some N metabolism genes,genes related to auxin and abscisic acid signal transduction in the roots of both genotypes,as well as genes related to gibberellin signal transduction in roots of LH.The N recovery treatment just increased the expression of genes related to cytokinin signal transduction in roots of LH.The expression levels of the NRT2.1,AMT1.1,and Gln1 genes related to gibberellin and cytokinin signal transduction were higher in roots of LH than in XF under different N treatments,suggesting that the genes related to N metabolism and hormone(auxin,abscisic acid,gibberellin,and cytokinin)signal transduction in roots of LH are more sensitive to different N treatments than those of XF.Co-expression network analysis(WGCNA)also identified hub genes like bZIP43,bHLH93,NPF6.3,IBR10,MYB62,PP2C,PP2C06 and NLP7,which may be the key regulators of N-mediated responses in chrysanthemum and play crucial roles in enhancing NUE and resistance to low N stress in the N-efficient chrysanthemum genotype.These results revealed the key factors involved in regulating NUE in chrysanthemum at the genetic level,which provides new insights into the complex mechanism of efficient nitrogen utilization in chrysanthemum,and can be useful for the improvement and breeding of high NUE chrysanthemum genotypes.展开更多
Nitrogen(N)is vital for crop growth and yield,impacting food quality.However,excessive use of N fertilizers leads to high agricultural costs and environmental challenges.This review offers a thorough synthesis of the ...Nitrogen(N)is vital for crop growth and yield,impacting food quality.However,excessive use of N fertilizers leads to high agricultural costs and environmental challenges.This review offers a thorough synthesis of the genetic and molecular regulation of N uptake,assimilation,and remobilization in maize,emphasizing the role of key genes and metabolic pathways in enhancing N use efficiency(NUE).We summarize the genetic regulators of N transports for nitrate(NO3−)and ammonium(NH4+)that contribute to efficient N uptake and transportation.We further discuss the molecular mechanisms by which root system development adapts to N distribution and how N influences root system development and growth.Given the advancements in high-throughput microbiome studies,we delve into the impact of rhizosphere microorganisms on NUE and the complex plant-microbe interactions that regulate maize NUE.Additionally,we conclude with intricate regulatory mechanisms of N assimilation and remobilization in maize,involving key enzymes,transcription factors,and amino acid transporters.We also scrutinize the known N signaling perception and transduction mechanisms in maize.This review underscores the challenges in improving maize NUE and advocates for an integrative research approach that leverages genetic diversity and synthetic biology,paving the way for sustainable agriculture.展开更多
Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high de...Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.展开更多
Nitrogen(N)fertilizer application is essential for crop-plant growth and development.Identifying genetic loci associated with N-use efficiency(NUE)could increase wheat yields and reduce environmental pollution caused ...Nitrogen(N)fertilizer application is essential for crop-plant growth and development.Identifying genetic loci associated with N-use efficiency(NUE)could increase wheat yields and reduce environmental pollution caused by overfertilization.We subjected a panel of 389 wheat accessions to N and chlorate(a nitrate analog)treatments to identify quantitative trait loci(QTL)controlling NUE-associated traits at the wheat seedling stage.Genotyping the panel with a 660K single-nucleotide polymorphism(SNP)array,we identified 397 SNPs associated with N-sensitivity index and chlorate inhibition rate.These SNPs were merged into 49 QTL,of which eight were multi-environment stable QTL and 27 were located near previously reported QTL.A set of 135 candidate genes near the 49 QTL included TaBOX(F-box family protein)and TaERF(ethylene-responsive transcription factor).A Tabox mutant was more sensitive to low-N stress than the wild-type plant.We developed two functional markers for Hap 1,the favorable allele of TaBOX.展开更多
基金National Natural Science Foundation of China No.30590381 No.31000211 National Basic Research Program of China No.2010CB833504
文摘From July 2008 to August 2008, 72 leaf samples from 22 species and 81 soil samples in the nine natural forest ecosystems were collected, from north to south along the North-South Transect of Eastern China （NSTEC）. Based on these samples, we studied the geographical distribution patterns of vegetable water use efficiency （WUE） and nitrogen use efficiency （NUE）, and analyzed their relationship with environmental factors. The vegetable WUE and NUE were calculated through the measurement of foliar δ 13C and C/N of predominant species, respectively. The results showed： （1） vegetable WUE, ranging from 2.13 to 28.67 mg C g-1 H2O, increased linearly from south to north in the representative forest ecosystems along the NSTEC, while vegetable NUE showed an opposite trend, increasing from north to south, ranging from 12.92 to 29.60 g C g-1 N. （2） Vegetable WUE and NUE were dominantly driven by climate and significantly affected by soil nutrient factors. Based on multiple stepwise regression analysis, mean annual temperature, soil phosphorus concentration, and soil nitrogen concentration were responding for 75.5% of the variations of WUE （p0.001）. While, mean annual precipitation and soil phosphorus concentration could explain 65.7% of the change in vegetable NUE （p0.001）. Moreover, vegetable WUE and NUE would also be seriously influenced by atmospheric nitrogen deposition in nitrogen saturated ecosystems. （3） There was a significant trade-off relationship between vegetable WUE and NUE in the typical forest ecosystems along the NSTEC （p0.001）, indicating a balanced strategy for vegetation in resource utilization in natural forest ecosystems along the NSTEC. This study suggests that global change would impact the resource use efficiency of forest ecosystems. However, vegetation could adapt to those changes by increasing the use efficiency of shortage resource while decreasing the relatively ample one. But extreme impacts, such as heavy nitrogen deposition, would break this trade-off mechanism and give a dramatic disturbance to the ecosystem biogeochemical cycle.
文摘Nitrogen （N） application before transplanting, where N fertilizers are applied in seedling-bed and carried to the paddy field with seedlings, is a novel method proposed in this article aiming for improving nitrogen utilization efficiency （NUE） in rice. The effect of this method on mineral N distribution in the rhizosphere soil was investigated in a field experiment with a japonica variety, Ningjing 2, in seasons of 2004 and 2005. There were four levels of N applied 16 h before transplanting： zero N （NO）, 207 kg ha^-1 （NL）, 310.5 kg ha^-1 （NM）, and 414 kg ha^-1 （NH）. The result indicated that N fertilizer before transplantation had positive effect of increasing mineral N content in the rhizosphere soil of rice. Generally, N content in the rhizosphere soil of rice tended to increase with the amount of N fertilizer before transplanting, with the NH treatment having the largest effect. Additionally, N fertilizer before transplanting had significant influence on rice NUE and grain yield. Compared with other treatments, the NM treatment showed the largest influence, with basal-tillering NUE, total NUE, and grain yield being 15%, 12%, and 529.5 kg ha^-1 higher than those of NO treatment. This result indicated that N fertilizer before transplantation had positive effect on mineral N distribution in the rhizosphere soil of rice, thus improving NUE and grain yield.
基金supported by the National Key R&D Programof China(2020YFD1000400)the National Natural Science Foundation of China(32072603)+1 种基金the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX(21)2004)the JBGS Project of Seed Industry Revitalization in Jiangsu Province,China(JBGS[2021]020).
文摘Nitrogen(N)is a limiting factor that determines the yield and quality of chrysanthemum.Genetic variation in N use efficiency(NUE)has been reported among chrysanthemum genotypes.We performed a transcriptome analysis of two chrysanthemum genotypes,'Nannonglihuang'(LH,N-efficient genotype)and'Nannongxuefeng"(XF,N-inefficient genotype),under low N(0.4 mmol L^(-1)N)and normal N(8 mmol L^(-1)N)treatments for 15 d and an N recovery treatment for 12 h(low N treatment for 15 d and then normal N treatment for 12 h)to understand the genetic factors impacting NUE in chrysanthemum.The two genotypes exhibited contrasting responses to the different N treatments.The N-efficient genotype LH had significant superiority in agronomic traits,N accumulation and glutamine synthase activity under both normal N and low N treatments.Low N treatment promoted root growth in LH,but inhibited root growth in XF.Transcriptome analysis revealed that the low N treatment increased the expression of some N metabolism genes,genes related to auxin and abscisic acid signal transduction in the roots of both genotypes,as well as genes related to gibberellin signal transduction in roots of LH.The N recovery treatment just increased the expression of genes related to cytokinin signal transduction in roots of LH.The expression levels of the NRT2.1,AMT1.1,and Gln1 genes related to gibberellin and cytokinin signal transduction were higher in roots of LH than in XF under different N treatments,suggesting that the genes related to N metabolism and hormone(auxin,abscisic acid,gibberellin,and cytokinin)signal transduction in roots of LH are more sensitive to different N treatments than those of XF.Co-expression network analysis(WGCNA)also identified hub genes like bZIP43,bHLH93,NPF6.3,IBR10,MYB62,PP2C,PP2C06 and NLP7,which may be the key regulators of N-mediated responses in chrysanthemum and play crucial roles in enhancing NUE and resistance to low N stress in the N-efficient chrysanthemum genotype.These results revealed the key factors involved in regulating NUE in chrysanthemum at the genetic level,which provides new insights into the complex mechanism of efficient nitrogen utilization in chrysanthemum,and can be useful for the improvement and breeding of high NUE chrysanthemum genotypes.
基金supported by the National Key Research and Development Program of China(2021YFF1000500 to L.Y.)the High-Level Talents Research Startup Fund of China Agricultural University(00114342 and 10092010 to J.L.)Pinduoduo-China Agricultural University Research Fund(PC2024B01009 to Z.J.).
文摘Nitrogen(N)is vital for crop growth and yield,impacting food quality.However,excessive use of N fertilizers leads to high agricultural costs and environmental challenges.This review offers a thorough synthesis of the genetic and molecular regulation of N uptake,assimilation,and remobilization in maize,emphasizing the role of key genes and metabolic pathways in enhancing N use efficiency(NUE).We summarize the genetic regulators of N transports for nitrate(NO3−)and ammonium(NH4+)that contribute to efficient N uptake and transportation.We further discuss the molecular mechanisms by which root system development adapts to N distribution and how N influences root system development and growth.Given the advancements in high-throughput microbiome studies,we delve into the impact of rhizosphere microorganisms on NUE and the complex plant-microbe interactions that regulate maize NUE.Additionally,we conclude with intricate regulatory mechanisms of N assimilation and remobilization in maize,involving key enzymes,transcription factors,and amino acid transporters.We also scrutinize the known N signaling perception and transduction mechanisms in maize.This review underscores the challenges in improving maize NUE and advocates for an integrative research approach that leverages genetic diversity and synthetic biology,paving the way for sustainable agriculture.
基金supported by Supported by National Key Laboratory of Cotton Bio-breeding and Integrated Utilization(CB2023C07)Xinjiang Autonomous Region"Three Agricultural"Backbone Talent Training Program(2022SNGGNT024)Xinjiang Huyanghe City Science and Technology Program(2023C08).
文摘Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.
基金This work was supported by the National Key Research and Development Program of China(2022YFD1200201)Henan Provincial Science and Technology Research and Development Plan Joint Fund(222301420025)the Agricultural Science and Technology Innovation Program(ASTIP)of CAAS.
文摘Nitrogen(N)fertilizer application is essential for crop-plant growth and development.Identifying genetic loci associated with N-use efficiency(NUE)could increase wheat yields and reduce environmental pollution caused by overfertilization.We subjected a panel of 389 wheat accessions to N and chlorate(a nitrate analog)treatments to identify quantitative trait loci(QTL)controlling NUE-associated traits at the wheat seedling stage.Genotyping the panel with a 660K single-nucleotide polymorphism(SNP)array,we identified 397 SNPs associated with N-sensitivity index and chlorate inhibition rate.These SNPs were merged into 49 QTL,of which eight were multi-environment stable QTL and 27 were located near previously reported QTL.A set of 135 candidate genes near the 49 QTL included TaBOX(F-box family protein)and TaERF(ethylene-responsive transcription factor).A Tabox mutant was more sensitive to low-N stress than the wild-type plant.We developed two functional markers for Hap 1,the favorable allele of TaBOX.
