Rauvolfia serpentina(L.)Benth.Ex Kurz is a greatly appreciated medicinal plant,well-known for its therapeutic benefits in traditional medicine,particularly in Ayurveda,where the roots and whole plant are used to treat...Rauvolfia serpentina(L.)Benth.Ex Kurz is a greatly appreciated medicinal plant,well-known for its therapeutic benefits in traditional medicine,particularly in Ayurveda,where the roots and whole plant are used to treat a variety of ailments.However,studies focusing on R.serpentina seeds are relatively scarce.Hence,the present study provides a novel approach by analysing the seed oil of R.serpentina extracted using the supercritical-carbon dioxide-fluid-extraction(SCFE)technique.The research employed advanced analytical methods including gas-chromatography with flame ionization detector(GC-FID),gas-chromatography-tandem mass spectrometry(GC-MS/MS),and high performance thin layer chromatography(HPTLC)to characterise the chemical composition of the extracted oil.Functional moieties were evaluated by Fourier transform infrared spectroscopy(FT-IR),while proton nuclear-magnetic-resonance(1H NMR)spectroscopy was utilised to identify the phytometabolites as well as to assess the physico-chemical parameters.The anti-microbial potential of the supercritically extracted oil was demonstrated through its activity against Klebsiella pneumoniae.The inhibitory effects on K.pneumoniae were quantified using the broth microdilution method,showing activity at both minimum inhibitory concentrations(MIC50 and MIC90).Furthermore,the oil was found to be non-genotoxic,as demonstrated by the Ames assay,which showed no mutagenic effects against S.typhimurium and E.coli WP2 uvrA.Since previous reports on R.serpentina seeds and their novel contribution in the field of pharmaceutics are rather limited,the present study is of utmost importance.The study may pave the way for future investigations into the therapeutic potentials of R.serpentina seeds.展开更多
We report the first nuclear magnetic resonance （NMR） study on single crystals of staircase Kagome antiferromagnet PbCu3Te07 （TN1 -36K）. A Curie constant θ -140K is obtained by a Curie Weiss fit to the high- tempe...We report the first nuclear magnetic resonance （NMR） study on single crystals of staircase Kagome antiferromagnet PbCu3Te07 （TN1 -36K）. A Curie constant θ -140K is obtained by a Curie Weiss fit to the high- temperature Knight shift of 125 Te. The hyperfine coupling constant is estimated to be 125Ahf = -67 kOe/#B, and a strong interlayer coupling among staircase Kagome planes is suggested with such a large hyperfine coupling, according to the lattice structure. The 63,65Cu NMR spectra are found by the zero-field （ZF） NMR at T = 2 K, and the internal hyperfine fields are estimated to be 10.3 T and 9.6 T, for Cu（1） and Cu（2） sites, respectively, in the lattice. A second type of ZF NMFt signal with a large rf enhancement is also seen after field-cycling through a high magnetic field.展开更多
基金Dr.Anupam Srivastava,Dr.Bhasker Joshi,Patanjali Herbal Research Division for their taxonomical supportsMr.Devendra Kumawat,Patanjali Research Foundation for his help in graphics+1 种基金Mr.Tarun Rajput,Mr.Gagan Kumar for their swift administrative supportsinternal research funds from Patanjali Research Foundation Trust,Haridwar,India.
文摘Rauvolfia serpentina(L.)Benth.Ex Kurz is a greatly appreciated medicinal plant,well-known for its therapeutic benefits in traditional medicine,particularly in Ayurveda,where the roots and whole plant are used to treat a variety of ailments.However,studies focusing on R.serpentina seeds are relatively scarce.Hence,the present study provides a novel approach by analysing the seed oil of R.serpentina extracted using the supercritical-carbon dioxide-fluid-extraction(SCFE)technique.The research employed advanced analytical methods including gas-chromatography with flame ionization detector(GC-FID),gas-chromatography-tandem mass spectrometry(GC-MS/MS),and high performance thin layer chromatography(HPTLC)to characterise the chemical composition of the extracted oil.Functional moieties were evaluated by Fourier transform infrared spectroscopy(FT-IR),while proton nuclear-magnetic-resonance(1H NMR)spectroscopy was utilised to identify the phytometabolites as well as to assess the physico-chemical parameters.The anti-microbial potential of the supercritically extracted oil was demonstrated through its activity against Klebsiella pneumoniae.The inhibitory effects on K.pneumoniae were quantified using the broth microdilution method,showing activity at both minimum inhibitory concentrations(MIC50 and MIC90).Furthermore,the oil was found to be non-genotoxic,as demonstrated by the Ames assay,which showed no mutagenic effects against S.typhimurium and E.coli WP2 uvrA.Since previous reports on R.serpentina seeds and their novel contribution in the field of pharmaceutics are rather limited,the present study is of utmost importance.The study may pave the way for future investigations into the therapeutic potentials of R.serpentina seeds.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11374364 and 11222433the National Basic Research Program of China under Grant No 2011CBA00112the Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry
文摘We report the first nuclear magnetic resonance （NMR） study on single crystals of staircase Kagome antiferromagnet PbCu3Te07 （TN1 -36K）. A Curie constant θ -140K is obtained by a Curie Weiss fit to the high- temperature Knight shift of 125 Te. The hyperfine coupling constant is estimated to be 125Ahf = -67 kOe/#B, and a strong interlayer coupling among staircase Kagome planes is suggested with such a large hyperfine coupling, according to the lattice structure. The 63,65Cu NMR spectra are found by the zero-field （ZF） NMR at T = 2 K, and the internal hyperfine fields are estimated to be 10.3 T and 9.6 T, for Cu（1） and Cu（2） sites, respectively, in the lattice. A second type of ZF NMFt signal with a large rf enhancement is also seen after field-cycling through a high magnetic field.
