NTL(NAC with transmembrane motif like)是普遍存在于单子叶和双子叶植物中的膜结合转录因子,在调控植物生长发育、逆境胁迫应答中发挥重要作用。NTL在石斛属(Dendrobium)植物中的生物学功能仍未知。本文在铁皮石斛(D.officinale)全基...NTL(NAC with transmembrane motif like)是普遍存在于单子叶和双子叶植物中的膜结合转录因子,在调控植物生长发育、逆境胁迫应答中发挥重要作用。NTL在石斛属(Dendrobium)植物中的生物学功能仍未知。本文在铁皮石斛(D.officinale)全基因组水平鉴定出7个NTL。利用生物信息学手段对DoNTL家族成员进行跨膜结构和系统进化分析,并预测蛋白质的二、三级结构以及蛋白质保守基序。通过逆转录PCR分析了DoNTL对不同胁迫的响应以及在根、茎、叶中的表达情况。结果显示,DoNTL基因在根、茎、叶中均有表达。胁迫处理下,NaCl诱导了NTL1、2、3、4、7的表达,抑制了NTL5的表达;脱落酸(ABA)诱导了NTL1、2、3、4的表达,抑制了NTL5的表达;聚乙二醇(PEG)诱导了NTL1、2、3、4、7的表达;高温抑制了NTL3、7的表达;低温诱导了NTL1、4的表达,抑制了NTL3的表达。表明DoNTL广泛参与非生物胁迫。这些结果为后续研究NTL在铁皮石斛生长发育过程中的功能提供数据支持。展开更多
vsx1(visual system homeobox-1),a homeobox gene originally identified from an adult goldfish retinal cDNA library,has been shown to regulate retina progenitor proliferation,differentiation and functional maintenance of...vsx1(visual system homeobox-1),a homeobox gene originally identified from an adult goldfish retinal cDNA library,has been shown to regulate retina progenitor proliferation,differentiation and functional maintenance of bipolar cells in vertebrates.However,in all the examined vertebrate species,vsx1 transcripts can be also detected at the early developmental stage,suggesting that it may play an important role in regulating early embryogenesis as well.Here,we investigated the function of vsx1 in early embryogenesis of goldfish(Carassius auratus) with both overexpression and gene knockdown approaches.It was found that vsx1 overexpression specifically blocked dorsal midline structure for-mation and vsx1 knockdown led to disorganized dorsal midline structure.Whole-mount in situ hy-bridization revealed that the midline expression of ntl,a key regulatory gene for chordamesoderm,was repressed by vsx1 overexpression but enhanced in the vsx1 knockdown.Furthermore,VSX1 protein could bind ntl promoter directly and was sufficient to inhibit ntl promoter-driven reporter gene green fluorescence protein transcription.Together,these results suggested that vsx1 may act to repress ec-topic expression of ntl in neural progenitor cells to ensure neural tube development in a spatially coordinated pattern during early embryogenesis.展开更多
文摘NTL(NAC with transmembrane motif like)是普遍存在于单子叶和双子叶植物中的膜结合转录因子,在调控植物生长发育、逆境胁迫应答中发挥重要作用。NTL在石斛属(Dendrobium)植物中的生物学功能仍未知。本文在铁皮石斛(D.officinale)全基因组水平鉴定出7个NTL。利用生物信息学手段对DoNTL家族成员进行跨膜结构和系统进化分析,并预测蛋白质的二、三级结构以及蛋白质保守基序。通过逆转录PCR分析了DoNTL对不同胁迫的响应以及在根、茎、叶中的表达情况。结果显示,DoNTL基因在根、茎、叶中均有表达。胁迫处理下,NaCl诱导了NTL1、2、3、4、7的表达,抑制了NTL5的表达;脱落酸(ABA)诱导了NTL1、2、3、4的表达,抑制了NTL5的表达;聚乙二醇(PEG)诱导了NTL1、2、3、4、7的表达;高温抑制了NTL3、7的表达;低温诱导了NTL1、4的表达,抑制了NTL3的表达。表明DoNTL广泛参与非生物胁迫。这些结果为后续研究NTL在铁皮石斛生长发育过程中的功能提供数据支持。
基金Supported by National Natural Science Foundation of China (Grant No.30430370)National Key Basic Research and Development Program of China (Grant No.2004CB117401)Doctoral Fund of Ministry of Education of China (Grant No.20050542002)
文摘vsx1(visual system homeobox-1),a homeobox gene originally identified from an adult goldfish retinal cDNA library,has been shown to regulate retina progenitor proliferation,differentiation and functional maintenance of bipolar cells in vertebrates.However,in all the examined vertebrate species,vsx1 transcripts can be also detected at the early developmental stage,suggesting that it may play an important role in regulating early embryogenesis as well.Here,we investigated the function of vsx1 in early embryogenesis of goldfish(Carassius auratus) with both overexpression and gene knockdown approaches.It was found that vsx1 overexpression specifically blocked dorsal midline structure for-mation and vsx1 knockdown led to disorganized dorsal midline structure.Whole-mount in situ hy-bridization revealed that the midline expression of ntl,a key regulatory gene for chordamesoderm,was repressed by vsx1 overexpression but enhanced in the vsx1 knockdown.Furthermore,VSX1 protein could bind ntl promoter directly and was sufficient to inhibit ntl promoter-driven reporter gene green fluorescence protein transcription.Together,these results suggested that vsx1 may act to repress ec-topic expression of ntl in neural progenitor cells to ensure neural tube development in a spatially coordinated pattern during early embryogenesis.
