SnoaL家族蛋白属于核转运因子2(nuclear transport factor 2,NTF2)样超家族,具有特征性的α+β折叠桶状结构。近年来研究发现该家族蛋白具有丰富的催化功能,能够催化羟醛缩合、羟化、脱羧、水解以及环加成等多种反应,在多种复杂结构活...SnoaL家族蛋白属于核转运因子2(nuclear transport factor 2,NTF2)样超家族,具有特征性的α+β折叠桶状结构。近年来研究发现该家族蛋白具有丰富的催化功能,能够催化羟醛缩合、羟化、脱羧、水解以及环加成等多种反应,在多种复杂结构活性天然产物的生物合成中发挥着重要作用。尽管这一家族蛋白广泛分布于细菌、真菌等生物体中,但目前针对该家族蛋白的研究还相对较少。本文通过系统综述SnoaL家族蛋白的研究进展,不仅能为后续相关蛋白的功能鉴定和机制研究提供参考,同时也有助于发现新颖的生物催化工具,为生物活性分子的合成生物学研究奠定基础。展开更多
The water-soluble radiolytic products of 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide ionic liquid([C2mim][NTf2]) under γ-radiation, such as CF3 SOOH, CF3SO2NH2, HF, and H2SO3, were identified by us...The water-soluble radiolytic products of 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide ionic liquid([C2mim][NTf2]) under γ-radiation, such as CF3 SOOH, CF3SO2NH2, HF, and H2SO3, were identified by using1 H NMR,19 F NMR, and ion chromatography. The extraction behavior of Dy3+using irradiated [C2mim][NTf2] in combination with 2,6-di(5,6-diisobutyl-1,2,4-triazin-3-yl)pyridine(isobutyl-BTP)was studied and the abnormal increase of Dy3+partitioning after irradiation is mainly attributed to the precipitation formed between Dy3+and radiolytic products of [C2mim][NTf2](F–and SO2–3). Washing irradiated[C2mim][NTf2] with water provides a simple method for ionic liquid recycling.展开更多
文摘SnoaL家族蛋白属于核转运因子2(nuclear transport factor 2,NTF2)样超家族,具有特征性的α+β折叠桶状结构。近年来研究发现该家族蛋白具有丰富的催化功能,能够催化羟醛缩合、羟化、脱羧、水解以及环加成等多种反应,在多种复杂结构活性天然产物的生物合成中发挥着重要作用。尽管这一家族蛋白广泛分布于细菌、真菌等生物体中,但目前针对该家族蛋白的研究还相对较少。本文通过系统综述SnoaL家族蛋白的研究进展,不仅能为后续相关蛋白的功能鉴定和机制研究提供参考,同时也有助于发现新颖的生物催化工具,为生物活性分子的合成生物学研究奠定基础。
基金Supported by the Ph.D.Programs Foundation of Ministry of Education of China(No.20130073120051)the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learningthe National Natural Science Foundation of China(No.11475112)
文摘The water-soluble radiolytic products of 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide ionic liquid([C2mim][NTf2]) under γ-radiation, such as CF3 SOOH, CF3SO2NH2, HF, and H2SO3, were identified by using1 H NMR,19 F NMR, and ion chromatography. The extraction behavior of Dy3+using irradiated [C2mim][NTf2] in combination with 2,6-di(5,6-diisobutyl-1,2,4-triazin-3-yl)pyridine(isobutyl-BTP)was studied and the abnormal increase of Dy3+partitioning after irradiation is mainly attributed to the precipitation formed between Dy3+and radiolytic products of [C2mim][NTf2](F–and SO2–3). Washing irradiated[C2mim][NTf2] with water provides a simple method for ionic liquid recycling.
