Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus （Narcissus tazetta var. chinensis Roem）, its flowers developed at high temperatures and bloomed at...Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus （Narcissus tazetta var. chinensis Roem）, its flowers developed at high temperatures and bloomed at lower temperatures during the Chinese Spring Festival, is a traditional Chinese flower with high economic and ornamental value. To study its flower development, a full length cDNA containing MADS box domain from narcissus was isolated by a reverse transcription polymerase chain reaction （RT-PCR） with degenerate oligo-nucleotide primers derived from a conserved MADS- and K-box domain sequence. The 5＇ and the 3＇ regions of the gene were amplified using the PCR protocol for the rapid amplification of cDNA ends （RACE）. The 690 bp open reading frame encodes 230 amino acid residues. A comparison of the deduced amino acid sequence of NTAG with the sequence of other MADS box proteins showed 91.3% amino acid identities with HAG （Hyacinthus orientalis）. Sequence analysis and alignment showed significant similarity with other AG homologues. RNA blot analysis indicated that the narcissus NTAG gene was expressed only in reproductive organs, especially in stamens and carpels. These results indicated that the NTAG gene was an AG homologue and that the AG genes appeared to be structurally and functionally conserved between dicots and monocots.展开更多
小批量近场通信标签(Near Field Communication Tag,NTAG)全自动读写设备利用由C#技术开发的上位机操作Excel文件进行数据处理,并驱动通用串行总线(Universal Serial BUS,USB)与下位机通信。机械部分根据料盘的结构特点设计了一种自适...小批量近场通信标签(Near Field Communication Tag,NTAG)全自动读写设备利用由C#技术开发的上位机操作Excel文件进行数据处理,并驱动通用串行总线(Universal Serial BUS,USB)与下位机通信。机械部分根据料盘的结构特点设计了一种自适应卡盘,传动部分根据NTAG的材料特点采用了限位开关与步进电机配合的方式,实现了精准定位。下位机以意法半导体系列单片机为核心,驱动电机芯片与射频识别传感器,实现了电机的变速控制与自动读写。展开更多
文摘Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus （Narcissus tazetta var. chinensis Roem）, its flowers developed at high temperatures and bloomed at lower temperatures during the Chinese Spring Festival, is a traditional Chinese flower with high economic and ornamental value. To study its flower development, a full length cDNA containing MADS box domain from narcissus was isolated by a reverse transcription polymerase chain reaction （RT-PCR） with degenerate oligo-nucleotide primers derived from a conserved MADS- and K-box domain sequence. The 5＇ and the 3＇ regions of the gene were amplified using the PCR protocol for the rapid amplification of cDNA ends （RACE）. The 690 bp open reading frame encodes 230 amino acid residues. A comparison of the deduced amino acid sequence of NTAG with the sequence of other MADS box proteins showed 91.3% amino acid identities with HAG （Hyacinthus orientalis）. Sequence analysis and alignment showed significant similarity with other AG homologues. RNA blot analysis indicated that the narcissus NTAG gene was expressed only in reproductive organs, especially in stamens and carpels. These results indicated that the NTAG gene was an AG homologue and that the AG genes appeared to be structurally and functionally conserved between dicots and monocots.
文摘小批量近场通信标签(Near Field Communication Tag,NTAG)全自动读写设备利用由C#技术开发的上位机操作Excel文件进行数据处理,并驱动通用串行总线(Universal Serial BUS,USB)与下位机通信。机械部分根据料盘的结构特点设计了一种自适应卡盘,传动部分根据NTAG的材料特点采用了限位开关与步进电机配合的方式,实现了精准定位。下位机以意法半导体系列单片机为核心,驱动电机芯片与射频识别传感器,实现了电机的变速控制与自动读写。
