为了探明NSG1(neuron specific gene family member 1,NSG1)基因与鸡繁殖性状的关联性,以找出可作为鸡繁殖性状的重要标记,试验选用贵州省地方鸡种荔波瑶山鸡,采用PCR测序法对NSG1基因进行变异检测,并进行与繁殖性状的关联分析。结果表...为了探明NSG1(neuron specific gene family member 1,NSG1)基因与鸡繁殖性状的关联性,以找出可作为鸡繁殖性状的重要标记,试验选用贵州省地方鸡种荔波瑶山鸡,采用PCR测序法对NSG1基因进行变异检测,并进行与繁殖性状的关联分析。结果表明:在荔波瑶山鸡NSG1基因上检测到2个变异位点,合并成2种单倍型。该单倍型与300日龄产蛋量、首次就巢持续时间、首次就巢至重新开产间隔、300日龄就巢次数存在极显著相关(P<0.01)。说明该位点可以作为荔波瑶山鸡繁殖性状选育的辅助选择标记。展开更多
【目的】探讨长梗秦艽酮、微小RNA(miR)-495-3p质粒对肾癌细胞增殖、侵袭、凋亡及顺铂敏感性的影响及协同作用。【方法】(1)体外研究:将人肾癌caki-1细胞分为空白组(未处理),长梗秦艽酮低、中、高剂量组,miR阴性对照(miRNC)组和miR-495...【目的】探讨长梗秦艽酮、微小RNA(miR)-495-3p质粒对肾癌细胞增殖、侵袭、凋亡及顺铂敏感性的影响及协同作用。【方法】(1)体外研究:将人肾癌caki-1细胞分为空白组(未处理),长梗秦艽酮低、中、高剂量组,miR阴性对照(miRNC)组和miR-495-3p组(转染组)等6组。细胞计数试剂盒8(CCK-8)法检测不同浓度顺铂处理的caki-1细胞活力、半数抑制浓度(IC50),膜联蛋白V-异硫氰酸荧光素(Annexin V-FITC)/碘化丙啶(PI)法测定caki-1细胞凋亡情况,Transwell实验测定caki-1细胞侵袭能力,实时定量聚合酶链反应(qRT-PCR)法检测细胞中miR-495-3p和X染色体连锁凋亡抑制蛋白(XIAP)mRNA表达。(2)体内研究:构建高度免疫缺陷NSG小鼠肾癌移植瘤模型。将40只NSG小鼠分为阴性组(腋部皮下注射转染miR-NC质粒的肾癌caki-1细胞)、转染组(腋部皮下注射转染miR-495-3p质粒的肾癌caki-1细胞)、药物组(腋部皮下注射肾癌caki-1细胞,灌胃长梗秦艽酮)和联合组(腋部皮下注射转染miR-495-3p质粒的肾癌caki-1细胞,灌胃长梗秦艽酮),检测并比较各组小鼠的肿瘤质量和体积。【结果】(1)与空白组比较,长梗秦艽酮低、中、高剂量组caki-1细胞活力及对顺铂的IC50值均显著降低,凋亡率显著增加,细胞侵袭能力显著降低,miR-495-3p表达水平升高,XIAP m RNA表达水平显著降低(均P<0.05);与miR-NC组比较,miR-495-3p组caki-1细胞活力、对顺铂的IC50值均显著降低,凋亡率显著增加,细胞侵袭能力显著降低,miR-495-3p表达水平升高,XIAP mRNA表达水平显著降低(均P<0.05);长梗秦艽酮高剂量组与miR-495-3p组上述各指标水平比较,差异均无统计学意义(P>0.05)。(2)与阴性组比较,转染组、药物组和联合组小鼠肿瘤质量和肿瘤体积均显著减小(P<0.05);联合组小鼠肿瘤质量和肿瘤体积均小于转染组和药物组(P<0.05);转染组小鼠肿瘤质量和肿瘤体积与药物组比较,差异无统计学意义(P>0.05)。【结论】长梗秦艽酮、miR-495-3p质粒可靶向抑制XIAP降低肾癌细胞生物活性,促进细胞凋亡,抑制细胞侵袭,增加细胞的顺铂敏感性,且二者有一定的协同作用。展开更多
Co_(0.9)Cu_(0.1)Si alloy was prepared by mechanical alloying method.Nitrogen-doped graphene(NG)and nitrogen–sulfur codoped graphene(NSG)were prepared by hydrothermal method.5 wt%graphene oxide,NG and NSG were doped i...Co_(0.9)Cu_(0.1)Si alloy was prepared by mechanical alloying method.Nitrogen-doped graphene(NG)and nitrogen–sulfur codoped graphene(NSG)were prepared by hydrothermal method.5 wt%graphene oxide,NG and NSG were doped into Co_(0.9)Cu_(0.1)Si alloy,respectively,by ball milling to improve the electrochemical hydrogen storage performance of the composite material.X-ray diffraction and scanning electron microscopy were used to characterize the structure and morphology of the composite material,and the LAND battery test system and three-electrode battery system were used to test the electrochemical performance of the composite material.The composite material showed better discharge capacity and better cycle stability than the pristine alloy.In addition,in order to study the optimal ratio of NSG,3%,5%,7%and 10%of NSG were doped into Co_(0.9)Cu_(0.1)Si alloy,respectively.Co_(0.9)Cu_(0.1)Si alloy doped with 5%NSG had the best performance among all the samples.The best discharge capacity was 580.1 mAh/g,and its highest capacity retention rate was 64.1%.The improvement in electrochemical hydrogen storage performance can be attributed to two aspects.On the one hand,the electrocatalytic performance of graphene is improved by co-doping nitrogen and sulfur,on the other hand,graphene has excellent electrical conductivity.展开更多
Objective: Aldehyde dehydrogenase (ALDH) enzymatic activity identifies ovarian cancer stem-like cells. We investigated the antineoplastic activity of the ALDH inhibitor Disulfiram on bulk ovarian cancer cells and CD13...Objective: Aldehyde dehydrogenase (ALDH) enzymatic activity identifies ovarian cancer stem-like cells. We investigated the antineoplastic activity of the ALDH inhibitor Disulfiram on bulk ovarian cancer cells and CD133+/ALDH+ cancer stem-like cells. Study Design: Ovarian cancer cell lines, human ovarian surface epithelial cells, and mesenchymal stem cells were treated with increasing concentrations of Disulfiram and/or Cisplatin in vitro. Treated cells were assessed for viability or FACS-analyzed for either percentage of ovarian cancer stem-like cells or induction of apoptosis. Disulfiram’s impact on cancer stem-like cells was tested in vitro using tumor sphere formation assays and in vivo using tumor initiation assays with in vitro-treated A2780 cells in NSG mice. Finally, Disulfiram’s in vivo activity was assessed versus CD133+/ALDH+ cell-initiated tumor xenografts. Results: Disulfiram demonstrated antineoplastic activity against multiple ovarian cancer cell lines. While Disulfiram had limited in vitro toxicity against human ovarian surface epithelial cells or mesenchymal stem cells (IC50 of ~15 μM and >30 μM, respectively), its antineoplastic activity against cell lines was comparable to Cisplatin (IC50 ~1.5 μM). Disulfiram-mediated cell death was due, at least in part, to induction of apoptosis. Disulfiram activity was additive with chemotherapy. Disulfiram demonstrated selective depletion of CD44+ cells but not the CD133+ cancer stem-like cells. Disulfiram had no therapeutic impact on tumor initiation studies or in vivo therapy of whole cell line or stem cell-initiated tumor xenografts. Conclusions: In biologically relevant concentrations, Disulfiram has clear antineoplastic activity against ovarian cancer cells in vitro. Disulfiram selectively depleted CD44+ but not CD133+ ovarian cancer stem-like cells in vitro. However, Disulfiram had no significant activity in vivo. Thus, improved and more selective ALDH inhibitors may be required to target ovarian cancer stem cells.展开更多
China-India relations have witnessed a gradual shift from ＂high anticipation＂ to ＂mutual disappointment＂ since Narendra Modi came to power in May 2014. Beginning in 2016, the bilateralrelations have entered the ＂...China-India relations have witnessed a gradual shift from ＂high anticipation＂ to ＂mutual disappointment＂ since Narendra Modi came to power in May 2014. Beginning in 2016, the bilateralrelations have entered the ＂troubled times＂ and turned chilled, as India has vented its anger on China for its failure to join the Nuclear Suppliers Group （NSG） and its escalating disputes with Pakistan. Although the two sides can still effectively manage their differences, the negative trend in the Sino- Indian relations calls for attention.展开更多
目的探究肿瘤坏死因子受体超家族成员4(tumor necrosis factor receptor superfamily member 4,OX40)单抗联合程序性死亡受体1(programmed cell death receptor 1,PD-1)单抗对子宫内膜癌病情进展以及肿瘤微环境中的白细胞分化抗原4+(clu...目的探究肿瘤坏死因子受体超家族成员4(tumor necrosis factor receptor superfamily member 4,OX40)单抗联合程序性死亡受体1(programmed cell death receptor 1,PD-1)单抗对子宫内膜癌病情进展以及肿瘤微环境中的白细胞分化抗原4+(cluster of differentiation 4^(+),CD4^(+))/白细胞分化抗原8+(cluster of differentiation 8^(+),CD8^(+))T细胞的影响。方法研究设计类型为动物体内前瞻性实验研究(随机对照设计)。往NSG小鼠左侧腋下注入子宫内膜癌细胞建立子宫内膜癌移植瘤模型。建模成功后将小鼠随机分为4组:空白处理组、PD-1单抗组、OX40单抗组、联合处理组,每组6只。通过静脉注射的方式往PD-1单抗组及联合处理组小鼠注射300μg的PD-1单抗,通过静脉注射的方式往OX40单抗组及联合处理组小鼠注射200μg的OX40单抗。于第28天剥离移植瘤组织并测量移植瘤体积和瘤重,并分析移植瘤组织的病理变化、肿瘤细胞的凋亡率、移植瘤组织中Bcl-2相关X蛋白(BAX)、B细胞淋巴瘤-2(Bcl-2)蛋白的表达以及CD4^(+)/CD8^(+)T细胞的数量变化。结果PD-1单抗组、OX40单抗组、联合处理组小鼠移植瘤的体积[(88.22±7.36)、(85.66±5.23)、(63.88±6.27)mm^(3)]、质量[(91.75±15.07)、(90.33±5.21)、(71.82±9.35)mg]、Bcl-2蛋白表达水平[(0.61±0.02)、(0.57±0.07)、(0.23±0.06)]均低于空白处理组[(113.19±5.73)mm^(3)、(121.77±11.98)mg、(0.78±0.06)],差异有统计学意义(F=63.595、21.132、101.936,均P<0.001),BAX蛋白表达水平[(0.62±0.05)、(0.59±0.07)、(0.91±0.09)]、肿瘤细胞凋亡率[(53.16±3.89)、(57.36±5.21)、(71.22±6.15)%]、CD4^(+)T细胞数量[(62.29±5.53)、(73.88±2.22)、(96.53±3.25)个/mm^(2)]、CD8^(+)T细胞数量[(112.07±13.27)、(119.55±17.91)、(173.87±15.69)个/mm^(2)]均高于空白处理组[(0.22±0.05)、(25.27±2.55)%、(26.57±6.73)个/mm^(2)、(73.27±11.28)个/mm^(2)],差异有统计学意义(F=106.711、102.884、223.900、47.326,均P<0.001)。联合处理组小鼠移植瘤的体积、质量、Bcl-2蛋白表达水平均低于PD-1单抗组、OX40单抗组,差异有统计学意义(F=26.648、6.514、88.180,均P<0.01),BAX蛋白表达水平、肿瘤细胞凋亡率、CD4^(+)T细胞数量、CD8^(+)T细胞数量均高于PD-1单抗组、OX40单抗组,差异有统计学意义(F=36.271、20.072、118.493、27.559,均P<0.001)。结论OX40单抗能够抑制小鼠子宫内膜癌移植瘤的进展,并能联合PD-1单抗协同增加CD4^(+)和CD8^(+)T细胞的数量进而促进肿瘤抑制作用。展开更多
New Delhi warms to the United States in a bid to acquire nuclear technologies for energy independence and strategic influence on July 21, U.S. Secretary of State Hillary Clinton completed her five-day trip to India. T...New Delhi warms to the United States in a bid to acquire nuclear technologies for energy independence and strategic influence on July 21, U.S. Secretary of State Hillary Clinton completed her five-day trip to India. The two sides reached several agree-ments during the visit. The most展开更多
PRO Supporters of the NSG’s decision argue that the policy change will keep nuclear technology and materials off the black market, ultimately making the world a safer place
文摘为了探明NSG1(neuron specific gene family member 1,NSG1)基因与鸡繁殖性状的关联性,以找出可作为鸡繁殖性状的重要标记,试验选用贵州省地方鸡种荔波瑶山鸡,采用PCR测序法对NSG1基因进行变异检测,并进行与繁殖性状的关联分析。结果表明:在荔波瑶山鸡NSG1基因上检测到2个变异位点,合并成2种单倍型。该单倍型与300日龄产蛋量、首次就巢持续时间、首次就巢至重新开产间隔、300日龄就巢次数存在极显著相关(P<0.01)。说明该位点可以作为荔波瑶山鸡繁殖性状选育的辅助选择标记。
文摘【目的】探讨长梗秦艽酮、微小RNA(miR)-495-3p质粒对肾癌细胞增殖、侵袭、凋亡及顺铂敏感性的影响及协同作用。【方法】(1)体外研究:将人肾癌caki-1细胞分为空白组(未处理),长梗秦艽酮低、中、高剂量组,miR阴性对照(miRNC)组和miR-495-3p组(转染组)等6组。细胞计数试剂盒8(CCK-8)法检测不同浓度顺铂处理的caki-1细胞活力、半数抑制浓度(IC50),膜联蛋白V-异硫氰酸荧光素(Annexin V-FITC)/碘化丙啶(PI)法测定caki-1细胞凋亡情况,Transwell实验测定caki-1细胞侵袭能力,实时定量聚合酶链反应(qRT-PCR)法检测细胞中miR-495-3p和X染色体连锁凋亡抑制蛋白(XIAP)mRNA表达。(2)体内研究:构建高度免疫缺陷NSG小鼠肾癌移植瘤模型。将40只NSG小鼠分为阴性组(腋部皮下注射转染miR-NC质粒的肾癌caki-1细胞)、转染组(腋部皮下注射转染miR-495-3p质粒的肾癌caki-1细胞)、药物组(腋部皮下注射肾癌caki-1细胞,灌胃长梗秦艽酮)和联合组(腋部皮下注射转染miR-495-3p质粒的肾癌caki-1细胞,灌胃长梗秦艽酮),检测并比较各组小鼠的肿瘤质量和体积。【结果】(1)与空白组比较,长梗秦艽酮低、中、高剂量组caki-1细胞活力及对顺铂的IC50值均显著降低,凋亡率显著增加,细胞侵袭能力显著降低,miR-495-3p表达水平升高,XIAP m RNA表达水平显著降低(均P<0.05);与miR-NC组比较,miR-495-3p组caki-1细胞活力、对顺铂的IC50值均显著降低,凋亡率显著增加,细胞侵袭能力显著降低,miR-495-3p表达水平升高,XIAP mRNA表达水平显著降低(均P<0.05);长梗秦艽酮高剂量组与miR-495-3p组上述各指标水平比较,差异均无统计学意义(P>0.05)。(2)与阴性组比较,转染组、药物组和联合组小鼠肿瘤质量和肿瘤体积均显著减小(P<0.05);联合组小鼠肿瘤质量和肿瘤体积均小于转染组和药物组(P<0.05);转染组小鼠肿瘤质量和肿瘤体积与药物组比较,差异无统计学意义(P>0.05)。【结论】长梗秦艽酮、miR-495-3p质粒可靶向抑制XIAP降低肾癌细胞生物活性,促进细胞凋亡,抑制细胞侵袭,增加细胞的顺铂敏感性,且二者有一定的协同作用。
基金This work is financially supported by the National Key R&D Program of China(No.2017YFE0198100)the Jilin Province Development Program of Science and Technology(Nos.20210509065RQ,20200401031GX)+3 种基金the Natural Science Foundation of Jilin Province(Nos.20200201254JC,20200201094JC)the Natural Science Foundation of Chongqing City(No.cstc2021jcyjmsxmX0243)the Research Project of the Education Department of Jilin Province(No.JJKH20220761KJ)the Scientific and Technological Project of Jilin Provincial Department of Education(No.JJKH20220763KJ).
文摘Co_(0.9)Cu_(0.1)Si alloy was prepared by mechanical alloying method.Nitrogen-doped graphene(NG)and nitrogen–sulfur codoped graphene(NSG)were prepared by hydrothermal method.5 wt%graphene oxide,NG and NSG were doped into Co_(0.9)Cu_(0.1)Si alloy,respectively,by ball milling to improve the electrochemical hydrogen storage performance of the composite material.X-ray diffraction and scanning electron microscopy were used to characterize the structure and morphology of the composite material,and the LAND battery test system and three-electrode battery system were used to test the electrochemical performance of the composite material.The composite material showed better discharge capacity and better cycle stability than the pristine alloy.In addition,in order to study the optimal ratio of NSG,3%,5%,7%and 10%of NSG were doped into Co_(0.9)Cu_(0.1)Si alloy,respectively.Co_(0.9)Cu_(0.1)Si alloy doped with 5%NSG had the best performance among all the samples.The best discharge capacity was 580.1 mAh/g,and its highest capacity retention rate was 64.1%.The improvement in electrochemical hydrogen storage performance can be attributed to two aspects.On the one hand,the electrocatalytic performance of graphene is improved by co-doping nitrogen and sulfur,on the other hand,graphene has excellent electrical conductivity.
文摘Objective: Aldehyde dehydrogenase (ALDH) enzymatic activity identifies ovarian cancer stem-like cells. We investigated the antineoplastic activity of the ALDH inhibitor Disulfiram on bulk ovarian cancer cells and CD133+/ALDH+ cancer stem-like cells. Study Design: Ovarian cancer cell lines, human ovarian surface epithelial cells, and mesenchymal stem cells were treated with increasing concentrations of Disulfiram and/or Cisplatin in vitro. Treated cells were assessed for viability or FACS-analyzed for either percentage of ovarian cancer stem-like cells or induction of apoptosis. Disulfiram’s impact on cancer stem-like cells was tested in vitro using tumor sphere formation assays and in vivo using tumor initiation assays with in vitro-treated A2780 cells in NSG mice. Finally, Disulfiram’s in vivo activity was assessed versus CD133+/ALDH+ cell-initiated tumor xenografts. Results: Disulfiram demonstrated antineoplastic activity against multiple ovarian cancer cell lines. While Disulfiram had limited in vitro toxicity against human ovarian surface epithelial cells or mesenchymal stem cells (IC50 of ~15 μM and >30 μM, respectively), its antineoplastic activity against cell lines was comparable to Cisplatin (IC50 ~1.5 μM). Disulfiram-mediated cell death was due, at least in part, to induction of apoptosis. Disulfiram activity was additive with chemotherapy. Disulfiram demonstrated selective depletion of CD44+ cells but not the CD133+ cancer stem-like cells. Disulfiram had no therapeutic impact on tumor initiation studies or in vivo therapy of whole cell line or stem cell-initiated tumor xenografts. Conclusions: In biologically relevant concentrations, Disulfiram has clear antineoplastic activity against ovarian cancer cells in vitro. Disulfiram selectively depleted CD44+ but not CD133+ ovarian cancer stem-like cells in vitro. However, Disulfiram had no significant activity in vivo. Thus, improved and more selective ALDH inhibitors may be required to target ovarian cancer stem cells.
文摘China-India relations have witnessed a gradual shift from ＂high anticipation＂ to ＂mutual disappointment＂ since Narendra Modi came to power in May 2014. Beginning in 2016, the bilateralrelations have entered the ＂troubled times＂ and turned chilled, as India has vented its anger on China for its failure to join the Nuclear Suppliers Group （NSG） and its escalating disputes with Pakistan. Although the two sides can still effectively manage their differences, the negative trend in the Sino- Indian relations calls for attention.
文摘目的探究肿瘤坏死因子受体超家族成员4(tumor necrosis factor receptor superfamily member 4,OX40)单抗联合程序性死亡受体1(programmed cell death receptor 1,PD-1)单抗对子宫内膜癌病情进展以及肿瘤微环境中的白细胞分化抗原4+(cluster of differentiation 4^(+),CD4^(+))/白细胞分化抗原8+(cluster of differentiation 8^(+),CD8^(+))T细胞的影响。方法研究设计类型为动物体内前瞻性实验研究(随机对照设计)。往NSG小鼠左侧腋下注入子宫内膜癌细胞建立子宫内膜癌移植瘤模型。建模成功后将小鼠随机分为4组:空白处理组、PD-1单抗组、OX40单抗组、联合处理组,每组6只。通过静脉注射的方式往PD-1单抗组及联合处理组小鼠注射300μg的PD-1单抗,通过静脉注射的方式往OX40单抗组及联合处理组小鼠注射200μg的OX40单抗。于第28天剥离移植瘤组织并测量移植瘤体积和瘤重,并分析移植瘤组织的病理变化、肿瘤细胞的凋亡率、移植瘤组织中Bcl-2相关X蛋白(BAX)、B细胞淋巴瘤-2(Bcl-2)蛋白的表达以及CD4^(+)/CD8^(+)T细胞的数量变化。结果PD-1单抗组、OX40单抗组、联合处理组小鼠移植瘤的体积[(88.22±7.36)、(85.66±5.23)、(63.88±6.27)mm^(3)]、质量[(91.75±15.07)、(90.33±5.21)、(71.82±9.35)mg]、Bcl-2蛋白表达水平[(0.61±0.02)、(0.57±0.07)、(0.23±0.06)]均低于空白处理组[(113.19±5.73)mm^(3)、(121.77±11.98)mg、(0.78±0.06)],差异有统计学意义(F=63.595、21.132、101.936,均P<0.001),BAX蛋白表达水平[(0.62±0.05)、(0.59±0.07)、(0.91±0.09)]、肿瘤细胞凋亡率[(53.16±3.89)、(57.36±5.21)、(71.22±6.15)%]、CD4^(+)T细胞数量[(62.29±5.53)、(73.88±2.22)、(96.53±3.25)个/mm^(2)]、CD8^(+)T细胞数量[(112.07±13.27)、(119.55±17.91)、(173.87±15.69)个/mm^(2)]均高于空白处理组[(0.22±0.05)、(25.27±2.55)%、(26.57±6.73)个/mm^(2)、(73.27±11.28)个/mm^(2)],差异有统计学意义(F=106.711、102.884、223.900、47.326,均P<0.001)。联合处理组小鼠移植瘤的体积、质量、Bcl-2蛋白表达水平均低于PD-1单抗组、OX40单抗组,差异有统计学意义(F=26.648、6.514、88.180,均P<0.01),BAX蛋白表达水平、肿瘤细胞凋亡率、CD4^(+)T细胞数量、CD8^(+)T细胞数量均高于PD-1单抗组、OX40单抗组,差异有统计学意义(F=36.271、20.072、118.493、27.559,均P<0.001)。结论OX40单抗能够抑制小鼠子宫内膜癌移植瘤的进展,并能联合PD-1单抗协同增加CD4^(+)和CD8^(+)T细胞的数量进而促进肿瘤抑制作用。
文摘New Delhi warms to the United States in a bid to acquire nuclear technologies for energy independence and strategic influence on July 21, U.S. Secretary of State Hillary Clinton completed her five-day trip to India. The two sides reached several agree-ments during the visit. The most
文摘PRO Supporters of the NSG’s decision argue that the policy change will keep nuclear technology and materials off the black market, ultimately making the world a safer place
