Porcine deltacoronavirus(PDCoV)is a swine enteropathogenic CoV that causes severe vomiting,diarrhea and dehydration in suckling piglets,leading to economic losses in the swine industry.There is a great need for a conv...Porcine deltacoronavirus(PDCoV)is a swine enteropathogenic CoV that causes severe vomiting,diarrhea and dehydration in suckling piglets,leading to economic losses in the swine industry.There is a great need for a convenient method to detect circulating antibodies and help in accurate diagnosis and disease control.Previously,we demonstrated that a unique PDCoV accessory protein,NS6,is expressed during PDCoV infection in pigs and is incorporated into PDCoV virions;thus,we deduced that NS6 is likely an immunogenic target that can be used for the diagnosis of PDCoV infection.In this study,we first confirmed that NS6 is immunogenic in PDCoV-infected pigs by perform-ing a serum western blot.Furthermore,we developed a novel NS6-based indirect enzyme-linked immunosorbent assay(iELISA)method and compared it to an established S1-based iELISA for the survey of anti-PDCoV IgG or IgA in pigs of different ages in China.The NS6-iELISA has high specificity for the detection of IgG antibodies and no cross-reactivity with other porcine enteric CoVs(transmissible gastroenteritis coronavirus,porcine epidemic diarrhea virus,or swine acute diarrhea syndrome coronavirus).This NS6 serology-based method has great sensitivity and good repeatability,making it a new and cost-saving option for the rapid diagnosis and immunosurveillance of PDCoV,which may also be important for the prevention and control of deltacoronavirus-related infection in pigs and other animals.展开更多
Large amount of disease-specific protein(SP) accumulated in the rice plant cells infected by rice grassy stunt virus(RGSV). It was deduced that the protein was encoded by NS6 gene on genomic vRNA6 and thus referred to...Large amount of disease-specific protein(SP) accumulated in the rice plant cells infected by rice grassy stunt virus(RGSV). It was deduced that the protein was encoded by NS6 gene on genomic vRNA6 and thus referred to as NS6 protein.But its function is unknown. In an effort to prove the above deduction and to elucidate the function of NS6 protein of RGSV, we constructed a bacterial expression plasmid pGTNS6 producing a fusion protein of glutathione S-transferase (GST) and NS6 protein, and a plant expression vector pCBTNSv6 containing NS6 gene. A recombinant plasmid pTNSv 6 containing the coding region of NS6 gene and the non-coding region at its 5’ terminus, cloned by RT-PCR from purified RNAs of Shaxian isolate of RGSV, was used as the start point. Western blot analysis showed that the fusion protein reacted strongly with antisera raised against RGSV-SP, which served as evidence of the deduction.EHA105 of Agrobacterium tumefasciens containing pCBTNSv6 has been obtained and the transformation of rice is underway.展开更多
The reaction of[CpMo(CO)_2]_2 with 8,8'-diquinolyl disulphide C_9H_6NSSNC_9H_6 resulted in the cleavage of Mo≡Mo triple bond to give a new nononuclear complex CpMo(CO)_2(N,S-C_9H_6NS)which was refluxed with PPh_3...The reaction of[CpMo(CO)_2]_2 with 8,8'-diquinolyl disulphide C_9H_6NSSNC_9H_6 resulted in the cleavage of Mo≡Mo triple bond to give a new nononuclear complex CpMo(CO)_2(N,S-C_9H_6NS)which was refluxed with PPh_3 in THF to form the title complex.The crystal structure of the title complex was determined by X-ray diffraction.展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation(LZ22C180002)the Laboratory of Lingnan Modern Agriculture Project(NG2022001)the Zhejiang Provincial Key R&D Program of China(2021C02049)。
文摘Porcine deltacoronavirus(PDCoV)is a swine enteropathogenic CoV that causes severe vomiting,diarrhea and dehydration in suckling piglets,leading to economic losses in the swine industry.There is a great need for a convenient method to detect circulating antibodies and help in accurate diagnosis and disease control.Previously,we demonstrated that a unique PDCoV accessory protein,NS6,is expressed during PDCoV infection in pigs and is incorporated into PDCoV virions;thus,we deduced that NS6 is likely an immunogenic target that can be used for the diagnosis of PDCoV infection.In this study,we first confirmed that NS6 is immunogenic in PDCoV-infected pigs by perform-ing a serum western blot.Furthermore,we developed a novel NS6-based indirect enzyme-linked immunosorbent assay(iELISA)method and compared it to an established S1-based iELISA for the survey of anti-PDCoV IgG or IgA in pigs of different ages in China.The NS6-iELISA has high specificity for the detection of IgG antibodies and no cross-reactivity with other porcine enteric CoVs(transmissible gastroenteritis coronavirus,porcine epidemic diarrhea virus,or swine acute diarrhea syndrome coronavirus).This NS6 serology-based method has great sensitivity and good repeatability,making it a new and cost-saving option for the rapid diagnosis and immunosurveillance of PDCoV,which may also be important for the prevention and control of deltacoronavirus-related infection in pigs and other animals.
文摘Large amount of disease-specific protein(SP) accumulated in the rice plant cells infected by rice grassy stunt virus(RGSV). It was deduced that the protein was encoded by NS6 gene on genomic vRNA6 and thus referred to as NS6 protein.But its function is unknown. In an effort to prove the above deduction and to elucidate the function of NS6 protein of RGSV, we constructed a bacterial expression plasmid pGTNS6 producing a fusion protein of glutathione S-transferase (GST) and NS6 protein, and a plant expression vector pCBTNSv6 containing NS6 gene. A recombinant plasmid pTNSv 6 containing the coding region of NS6 gene and the non-coding region at its 5’ terminus, cloned by RT-PCR from purified RNAs of Shaxian isolate of RGSV, was used as the start point. Western blot analysis showed that the fusion protein reacted strongly with antisera raised against RGSV-SP, which served as evidence of the deduction.EHA105 of Agrobacterium tumefasciens containing pCBTNSv6 has been obtained and the transformation of rice is underway.
文摘The reaction of[CpMo(CO)_2]_2 with 8,8'-diquinolyl disulphide C_9H_6NSSNC_9H_6 resulted in the cleavage of Mo≡Mo triple bond to give a new nononuclear complex CpMo(CO)_2(N,S-C_9H_6NS)which was refluxed with PPh_3 in THF to form the title complex.The crystal structure of the title complex was determined by X-ray diffraction.
