The full-length Mlo gene was obtained by reverse transcription polymerase chain reaction （RT-PCR） and RACE. The result of sequence analysis indicated that M/o gene from Pericallis hybrida B. Nord. contained about 12...The full-length Mlo gene was obtained by reverse transcription polymerase chain reaction （RT-PCR） and RACE. The result of sequence analysis indicated that M/o gene from Pericallis hybrida B. Nord. contained about 1296bp open reading frame and encoded 431 amino acids. According to the comparison of the exogenous gene sequences by BLAST analysis and phylogenetic analysis, Mlo gene shared over 85% nucleotide homology and 98% amino acid homology. Finally, through semi-quantitative-PCR and fluorescence quantitative analysis, we found that Mlo gene showed the highest expression levels in leaves and the lowest in roots after inoculated with powdery mildew pathogen for different days.展开更多
Unanticipated sabotage of two underwater pipelines in the Baltic Sea(Nord Stream 1 and 2)happened on 26September 2022.Massive quantities of natural gas,primarily methane,were released into the atmosphere,which lasted ...Unanticipated sabotage of two underwater pipelines in the Baltic Sea(Nord Stream 1 and 2)happened on 26September 2022.Massive quantities of natural gas,primarily methane,were released into the atmosphere,which lasted for about one week.As a more powerful greenhouse gas than CO_(2),the potential climatic impact of methane is a global concern.Using multiple methods and datasets,a recent study reported a relatively accurate magnitude of the leaked methane at 0.22±0.03 million tons(Mt),which was lower than the initial estimate in the immediate aftermath of the event.Under an energy conservation framework used in IPCC AR6,we derived a negligible increase in global surface air temperature of 1.8×10^(-5)℃ in a 20-year time horizon caused by the methane leaks with an upper limit of 0.25 Mt.Although the resultant warming from this methane leak incident was minor,future carbon release from additional Earth system feedbacks,such as thawing permafrost,and its impact on the methane mitigation pathways of the Paris Agreement,warrants investigation.展开更多
Introduction: European association of urology (EAU), American Urology Association (AUA) and French Association of Urology (AFU) recommend either Extracorporeal Lithotripsy or Ureteroscopy for the surgical management o...Introduction: European association of urology (EAU), American Urology Association (AUA) and French Association of Urology (AFU) recommend either Extracorporeal Lithotripsy or Ureteroscopy for the surgical management of stones smaller than 2 cm in size. The aim of this study was to analyse the management of upper urinary tract stones by ureteroscopy in the urology department of the Nord Franche Comte Hospital. Methods: This was a prospective, descriptive, single-centre study lasting seven (07) months from 02 November 2021 to 31 May 2022 and involving 71 patients treated for lithiasis of the upper urinary tract by ureteroscopy. The preoperative, preoperative and postoperative characteristics of the patients and the disease, including the radiological control results, were studied. Results: One hundred and four stones were treated, the mean age was 55.94 ± 17.22 years, the sex ratio M/F 1.84;the circumstances of discovery were renal colic in 73.2%. The mean size of the stones was 9.12 mm;mean density 951.45 HU;the left side was most affected in 54.93% of cases. The stone was unique in 69.01% of cases. The preoperative Cytobacterioligical urine exam (CBUE) was positive in 15.5% of patients. Préstinting was performed in 84.5% of patients. We performed 45.1% rigid Ureteroscopy and 39.4% flexible Ureteroscopy. The average duration of the procedure was 46.63 min. Monobloc stone extraction was 35.2%, laser fragmentation 31%. Drainage was not performed in 50.7% of patients. The outpatient and postoperative complication rates were 87.3% and 9.86%, respectively. The stone-free rate was 90.1%. Conclusion: This initial evaluation of Ureteroscopy at Nord Franche Comte Hospital (HNFC), although based on a small sample, is satisfactory and in line with the literature.展开更多
The modeling of volatility and correlation is important in order to calculate hedge ratios, value at risk estimates, CAPM （Capital Asset Pricing Model betas）, derivate pricing and risk management in general. Recent ...The modeling of volatility and correlation is important in order to calculate hedge ratios, value at risk estimates, CAPM （Capital Asset Pricing Model betas）, derivate pricing and risk management in general. Recent access to intra-daily high-frequency data for two of the most liquid contracts at the Nord Pool exchange has made it possible to apply new and promising methods for analyzing volatility and correlation. The concepts of realized volatility and realized correlation are applied, and this study statistically describes the distribution （both distributional properties and temporal dependencies） of electricity forward data from 2005 to 2009. The main findings show that the logarithmic realized volatility is approximately normally distributed, while realized correlation seems not to be. Further, realized volatility and realized correlation have a long-memory feature. There also seems to be a high correlation between realized correlation and volatilities and positive relations between trading volume and realized volatility and between trading volume and realized correlation. These results are to a large extent consistent with earlier studies of stylized facts of other financial and commodity markets.展开更多
The American Striped Cray Orconectes limosus(Rafinesque),a nearctic crustacean new in theitalian freshwaters(Crustacea Decapoda Cambaridae).The American Striped Cray Orconectes limosus,introduced in several countries ...The American Striped Cray Orconectes limosus(Rafinesque),a nearctic crustacean new in theitalian freshwaters(Crustacea Decapoda Cambaridae).The American Striped Cray Orconectes limosus,introduced in several countries of Central Europe,is also reported for the first time for Italy.This crayfishseems to become well established in the Iseo Lake(Northern Italy).The Author underlines the risk of a possibleterritorial expansion of this species in the Po river drainage.展开更多
2022年北溪天然气管道泄漏事件引起了全世界的广泛关注。建立了北溪天然气管道的有限元分析(finite element analysis,简称FEA)模型,使用LS-DYNA求解器和ALE(arbitrary Lagrangian-Eulerian)算法模拟了水下爆炸及管道破坏的全过程,并通...2022年北溪天然气管道泄漏事件引起了全世界的广泛关注。建立了北溪天然气管道的有限元分析(finite element analysis,简称FEA)模型,使用LS-DYNA求解器和ALE(arbitrary Lagrangian-Eulerian)算法模拟了水下爆炸及管道破坏的全过程,并通过了模型验证。参考北溪管道事件中泄漏点的实地拍摄照片,通过有限元分析实现了事故反演,并从中分析推断得出造成北溪一号和北溪二号管道泄漏的可能原因。研究明晰了内部承压海底管道在水下爆炸作用下毁伤的3个阶段:爆炸冲击阶段、内压泄漏阶段和稳定阶段。引入中空夹层复合海底管道结构形式,并对其进行了水下爆炸的有限元分析。分析结果显示,新型结构相比于常用的单层管道形式,可显著提升其抗爆和可修复性能,为相关海底管道抗爆设计提供优选结构形式。展开更多
基金Supported by Postdoctoral Scientifi c Research Foundation of Heilongjiang Province(LBH-Q10144)the Natural Science Foundation of Heilongjiang Province(C201112)Northeast Agricultural University Doctoral Research Fund(200830)
文摘The full-length Mlo gene was obtained by reverse transcription polymerase chain reaction （RT-PCR） and RACE. The result of sequence analysis indicated that M/o gene from Pericallis hybrida B. Nord. contained about 1296bp open reading frame and encoded 431 amino acids. According to the comparison of the exogenous gene sequences by BLAST analysis and phylogenetic analysis, Mlo gene shared over 85% nucleotide homology and 98% amino acid homology. Finally, through semi-quantitative-PCR and fluorescence quantitative analysis, we found that Mlo gene showed the highest expression levels in leaves and the lowest in roots after inoculated with powdery mildew pathogen for different days.
基金supported by the National Key Research and Development Program(Grant No.2017YFA0603503)the National Natural Science Foundation of China(Grant No.41605057)。
文摘Unanticipated sabotage of two underwater pipelines in the Baltic Sea(Nord Stream 1 and 2)happened on 26September 2022.Massive quantities of natural gas,primarily methane,were released into the atmosphere,which lasted for about one week.As a more powerful greenhouse gas than CO_(2),the potential climatic impact of methane is a global concern.Using multiple methods and datasets,a recent study reported a relatively accurate magnitude of the leaked methane at 0.22±0.03 million tons(Mt),which was lower than the initial estimate in the immediate aftermath of the event.Under an energy conservation framework used in IPCC AR6,we derived a negligible increase in global surface air temperature of 1.8×10^(-5)℃ in a 20-year time horizon caused by the methane leaks with an upper limit of 0.25 Mt.Although the resultant warming from this methane leak incident was minor,future carbon release from additional Earth system feedbacks,such as thawing permafrost,and its impact on the methane mitigation pathways of the Paris Agreement,warrants investigation.
文摘Introduction: European association of urology (EAU), American Urology Association (AUA) and French Association of Urology (AFU) recommend either Extracorporeal Lithotripsy or Ureteroscopy for the surgical management of stones smaller than 2 cm in size. The aim of this study was to analyse the management of upper urinary tract stones by ureteroscopy in the urology department of the Nord Franche Comte Hospital. Methods: This was a prospective, descriptive, single-centre study lasting seven (07) months from 02 November 2021 to 31 May 2022 and involving 71 patients treated for lithiasis of the upper urinary tract by ureteroscopy. The preoperative, preoperative and postoperative characteristics of the patients and the disease, including the radiological control results, were studied. Results: One hundred and four stones were treated, the mean age was 55.94 ± 17.22 years, the sex ratio M/F 1.84;the circumstances of discovery were renal colic in 73.2%. The mean size of the stones was 9.12 mm;mean density 951.45 HU;the left side was most affected in 54.93% of cases. The stone was unique in 69.01% of cases. The preoperative Cytobacterioligical urine exam (CBUE) was positive in 15.5% of patients. Préstinting was performed in 84.5% of patients. We performed 45.1% rigid Ureteroscopy and 39.4% flexible Ureteroscopy. The average duration of the procedure was 46.63 min. Monobloc stone extraction was 35.2%, laser fragmentation 31%. Drainage was not performed in 50.7% of patients. The outpatient and postoperative complication rates were 87.3% and 9.86%, respectively. The stone-free rate was 90.1%. Conclusion: This initial evaluation of Ureteroscopy at Nord Franche Comte Hospital (HNFC), although based on a small sample, is satisfactory and in line with the literature.
文摘The modeling of volatility and correlation is important in order to calculate hedge ratios, value at risk estimates, CAPM （Capital Asset Pricing Model betas）, derivate pricing and risk management in general. Recent access to intra-daily high-frequency data for two of the most liquid contracts at the Nord Pool exchange has made it possible to apply new and promising methods for analyzing volatility and correlation. The concepts of realized volatility and realized correlation are applied, and this study statistically describes the distribution （both distributional properties and temporal dependencies） of electricity forward data from 2005 to 2009. The main findings show that the logarithmic realized volatility is approximately normally distributed, while realized correlation seems not to be. Further, realized volatility and realized correlation have a long-memory feature. There also seems to be a high correlation between realized correlation and volatilities and positive relations between trading volume and realized volatility and between trading volume and realized correlation. These results are to a large extent consistent with earlier studies of stylized facts of other financial and commodity markets.
文摘The American Striped Cray Orconectes limosus(Rafinesque),a nearctic crustacean new in theitalian freshwaters(Crustacea Decapoda Cambaridae).The American Striped Cray Orconectes limosus,introduced in several countries of Central Europe,is also reported for the first time for Italy.This crayfishseems to become well established in the Iseo Lake(Northern Italy).The Author underlines the risk of a possibleterritorial expansion of this species in the Po river drainage.
文摘2022年北溪天然气管道泄漏事件引起了全世界的广泛关注。建立了北溪天然气管道的有限元分析(finite element analysis,简称FEA)模型,使用LS-DYNA求解器和ALE(arbitrary Lagrangian-Eulerian)算法模拟了水下爆炸及管道破坏的全过程,并通过了模型验证。参考北溪管道事件中泄漏点的实地拍摄照片,通过有限元分析实现了事故反演,并从中分析推断得出造成北溪一号和北溪二号管道泄漏的可能原因。研究明晰了内部承压海底管道在水下爆炸作用下毁伤的3个阶段:爆炸冲击阶段、内压泄漏阶段和稳定阶段。引入中空夹层复合海底管道结构形式,并对其进行了水下爆炸的有限元分析。分析结果显示,新型结构相比于常用的单层管道形式,可显著提升其抗爆和可修复性能,为相关海底管道抗爆设计提供优选结构形式。
