Objective: To establish a method to improve the detection of disseminated tumor cells in bone marrow and peripheral blood samples of neuroblastoma patients and analysis of cytogenetic aberration. Methods: Immunofluore...Objective: To establish a method to improve the detection of disseminated tumor cells in bone marrow and peripheral blood samples of neuroblastoma patients and analysis of cytogenetic aberration. Methods: Immunofluorescent staining was performed using a cocktail of primary monoclonal neuroblastoma antibodies (14.G2a, 5.1H11). Fluorescence in situ hybridization was applied with fluorescent probes specific for Nmyc genes afterwards. A novel computer assisted scanning system for automatic search, image analysis and repositioning of these positive cells was developed. Fifty-six bone marrow and peripheral blood samples from 7 patients were evaluated by this method. Results: Fluorescence in situ hybridization can be combined with immunofluorescent staining in detecting Nmyc amplification in neuroblastoma patients. Fluorescence in situ hybridization results correlated well with data obtained by conventional cytogenetic procedures. Conclusion: The technique described allows search of tumor cells in the bone marrow as well as detection of Nmyc amplification in interphase nuclei.展开更多
Objective To investigate the mechanisms underlying elemene-induced analgesia in rats with spared nerve injury(SNI).Methods Sixty-five rats were equally divided into 5 groups using a random number table:naive group,sha...Objective To investigate the mechanisms underlying elemene-induced analgesia in rats with spared nerve injury(SNI).Methods Sixty-five rats were equally divided into 5 groups using a random number table:naive group,sham group,SNI group,SNI+elemene(40 mg·kg−1·d−1)group and naive+elemene(40 mg·kg−1·d−1)group.An SNI rat model was established and the intervention were given respectively for 14 consecutive days.Von Frey filament tests and elevated plus-maze(EPM)tests were used to evaluate the effect of elemene on the mechanical threshold and anxiety,respectively.Immunoblotting and immunostaining were used to measure the expression of glial fibrillary acidic protein(GFAP)and NMYC downstream-regulated gene 2(NDRG2)within the lumbar spinal dorsal horn(SDH).Results The SNI rat model exhibited a significant decrease in paw withdrawal threshold and exploratory behaviour in the EPM(P<0.05).Consecutive administration of elemene alleviated SNI-induced mechanical allodynia and anxiety in rats(P<0.05).Immunohistochemical data showed that elemene decreased SNI-induced upregulation of NDRG2 within the SDH(P<0.05).Double immunofluorescent staining data further showed that elemene decreased SNI-induced upregulation of the number of GFAP immunoreactive(-ir),NDRG-ir,and GFAP/NDRG2 double-labelled cells within the SDH(P<0.05).Immunoblotting data showed that elemene decreased SNI-induced upregulation of GFAP and NDRG2 within the SDH(P<0.05).Conclusion Elemene possibly alleviated neuropathic pain by downregulating the expression of NDRG2 in spinal astrocytes in a rat model of SNI.展开更多
基金This work was supported by a grant fromthe CSC scholarship of the Ministry of Education ofChina(No. 99837010)
文摘Objective: To establish a method to improve the detection of disseminated tumor cells in bone marrow and peripheral blood samples of neuroblastoma patients and analysis of cytogenetic aberration. Methods: Immunofluorescent staining was performed using a cocktail of primary monoclonal neuroblastoma antibodies (14.G2a, 5.1H11). Fluorescence in situ hybridization was applied with fluorescent probes specific for Nmyc genes afterwards. A novel computer assisted scanning system for automatic search, image analysis and repositioning of these positive cells was developed. Fifty-six bone marrow and peripheral blood samples from 7 patients were evaluated by this method. Results: Fluorescence in situ hybridization can be combined with immunofluorescent staining in detecting Nmyc amplification in neuroblastoma patients. Fluorescence in situ hybridization results correlated well with data obtained by conventional cytogenetic procedures. Conclusion: The technique described allows search of tumor cells in the bone marrow as well as detection of Nmyc amplification in interphase nuclei.
基金the Science and Technology Co-ordination Innovation Project Plan of Shaanxi Province(No.2016KTCL03-16)。
文摘Objective To investigate the mechanisms underlying elemene-induced analgesia in rats with spared nerve injury(SNI).Methods Sixty-five rats were equally divided into 5 groups using a random number table:naive group,sham group,SNI group,SNI+elemene(40 mg·kg−1·d−1)group and naive+elemene(40 mg·kg−1·d−1)group.An SNI rat model was established and the intervention were given respectively for 14 consecutive days.Von Frey filament tests and elevated plus-maze(EPM)tests were used to evaluate the effect of elemene on the mechanical threshold and anxiety,respectively.Immunoblotting and immunostaining were used to measure the expression of glial fibrillary acidic protein(GFAP)and NMYC downstream-regulated gene 2(NDRG2)within the lumbar spinal dorsal horn(SDH).Results The SNI rat model exhibited a significant decrease in paw withdrawal threshold and exploratory behaviour in the EPM(P<0.05).Consecutive administration of elemene alleviated SNI-induced mechanical allodynia and anxiety in rats(P<0.05).Immunohistochemical data showed that elemene decreased SNI-induced upregulation of NDRG2 within the SDH(P<0.05).Double immunofluorescent staining data further showed that elemene decreased SNI-induced upregulation of the number of GFAP immunoreactive(-ir),NDRG-ir,and GFAP/NDRG2 double-labelled cells within the SDH(P<0.05).Immunoblotting data showed that elemene decreased SNI-induced upregulation of GFAP and NDRG2 within the SDH(P<0.05).Conclusion Elemene possibly alleviated neuropathic pain by downregulating the expression of NDRG2 in spinal astrocytes in a rat model of SNI.
